Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_6020

Experiment: Choline Lysine 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_6018 and Pf6N2E2_6019 are separated by 21 nucleotidesPf6N2E2_6019 and Pf6N2E2_6020 are separated by 194 nucleotidesPf6N2E2_6020 and Pf6N2E2_6021 are separated by 144 nucleotidesPf6N2E2_6021 and Pf6N2E2_6022 overlap by 11 nucleotidesPf6N2E2_6022 and Pf6N2E2_6023 are separated by 4 nucleotides Pf6N2E2_6018 - Fumarylacetoacetate hydrolase family protein, at 6,838,446 to 6,839,144 _6018 Pf6N2E2_6019 - putative oxidoreductase, at 6,839,166 to 6,839,924 _6019 Pf6N2E2_6020 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 6,840,119 to 6,841,075 _6020 Pf6N2E2_6021 - RelB/StbD replicon stabilization protein (antitoxin to RelE/StbE), at 6,841,220 to 6,841,507 _6021 Pf6N2E2_6022 - StbE replicon stabilization toxin, at 6,841,497 to 6,841,808 _6022 Pf6N2E2_6023 - Putative cytoplasmic protein, at 6,841,813 to 6,842,451 _6023 Position (kb) 6840 6841 6842Strain fitness (log2 ratio) -2 -1 0 1 2 3at 6839.139 kb on + strandat 6839.139 kb on + strandat 6839.253 kb on + strand, within Pf6N2E2_6019at 6839.353 kb on + strand, within Pf6N2E2_6019at 6839.354 kb on - strand, within Pf6N2E2_6019at 6839.354 kb on - strand, within Pf6N2E2_6019at 6839.354 kb on - strand, within Pf6N2E2_6019at 6839.403 kb on + strand, within Pf6N2E2_6019at 6839.404 kb on - strand, within Pf6N2E2_6019at 6839.697 kb on + strand, within Pf6N2E2_6019at 6839.697 kb on + strand, within Pf6N2E2_6019at 6839.697 kb on + strand, within Pf6N2E2_6019at 6839.698 kb on - strand, within Pf6N2E2_6019at 6839.698 kb on - strand, within Pf6N2E2_6019at 6839.698 kb on - strand, within Pf6N2E2_6019at 6839.698 kb on - strand, within Pf6N2E2_6019at 6839.770 kb on + strand, within Pf6N2E2_6019at 6839.770 kb on + strand, within Pf6N2E2_6019at 6839.771 kb on - strand, within Pf6N2E2_6019at 6839.842 kb on - strand, within Pf6N2E2_6019at 6840.107 kb on + strandat 6840.230 kb on + strand, within Pf6N2E2_6020at 6840.230 kb on + strand, within Pf6N2E2_6020at 6840.230 kb on + strand, within Pf6N2E2_6020at 6840.231 kb on - strand, within Pf6N2E2_6020at 6840.231 kb on - strand, within Pf6N2E2_6020at 6840.231 kb on - strand, within Pf6N2E2_6020at 6840.231 kb on - strand, within Pf6N2E2_6020at 6840.231 kb on - strand, within Pf6N2E2_6020at 6840.450 kb on + strand, within Pf6N2E2_6020at 6840.451 kb on - strand, within Pf6N2E2_6020at 6840.647 kb on + strand, within Pf6N2E2_6020at 6840.647 kb on + strand, within Pf6N2E2_6020at 6840.647 kb on + strand, within Pf6N2E2_6020at 6840.647 kb on + strand, within Pf6N2E2_6020at 6840.647 kb on + strand, within Pf6N2E2_6020at 6840.807 kb on - strand, within Pf6N2E2_6020at 6841.033 kb on + strandat 6841.034 kb on - strandat 6841.034 kb on - strandat 6841.034 kb on - strandat 6841.135 kb on + strandat 6841.135 kb on + strandat 6841.135 kb on + strandat 6841.135 kb on + strandat 6841.135 kb on + strandat 6841.135 kb on + strandat 6841.135 kb on + strandat 6841.136 kb on - strandat 6841.198 kb on + strandat 6841.198 kb on + strandat 6841.509 kb on + strandat 6841.509 kb on + strandat 6841.509 kb on + strandat 6841.509 kb on + strandat 6841.509 kb on + strandat 6841.653 kb on + strand, within Pf6N2E2_6022at 6841.653 kb on + strand, within Pf6N2E2_6022at 6841.692 kb on + strand, within Pf6N2E2_6022at 6841.787 kb on + strandat 6841.788 kb on - strandat 6841.865 kb on + strandat 6841.866 kb on - strandat 6841.866 kb on - strandat 6841.866 kb on - strandat 6841.866 kb on - strandat 6841.928 kb on - strand, within Pf6N2E2_6023

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline Lysine 20 mM
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6,839,139 + -1.5
6,839,139 + -0.4
6,839,253 + Pf6N2E2_6019 0.11 +0.2
6,839,353 + Pf6N2E2_6019 0.25 +0.5
6,839,354 - Pf6N2E2_6019 0.25 +0.0
6,839,354 - Pf6N2E2_6019 0.25 +0.4
6,839,354 - Pf6N2E2_6019 0.25 +0.7
6,839,403 + Pf6N2E2_6019 0.31 -0.2
6,839,404 - Pf6N2E2_6019 0.31 -0.2
6,839,697 + Pf6N2E2_6019 0.70 -0.3
6,839,697 + Pf6N2E2_6019 0.70 +0.8
6,839,697 + Pf6N2E2_6019 0.70 +0.1
6,839,698 - Pf6N2E2_6019 0.70 -0.8
6,839,698 - Pf6N2E2_6019 0.70 +0.0
6,839,698 - Pf6N2E2_6019 0.70 +1.0
6,839,698 - Pf6N2E2_6019 0.70 -0.4
6,839,770 + Pf6N2E2_6019 0.80 -0.5
6,839,770 + Pf6N2E2_6019 0.80 -0.5
6,839,771 - Pf6N2E2_6019 0.80 -0.1
6,839,842 - Pf6N2E2_6019 0.89 +1.0
6,840,107 + +0.5
6,840,230 + Pf6N2E2_6020 0.12 -0.1
6,840,230 + Pf6N2E2_6020 0.12 -1.5
6,840,230 + Pf6N2E2_6020 0.12 +0.5
6,840,231 - Pf6N2E2_6020 0.12 +1.0
6,840,231 - Pf6N2E2_6020 0.12 -0.1
6,840,231 - Pf6N2E2_6020 0.12 +0.6
6,840,231 - Pf6N2E2_6020 0.12 -0.1
6,840,231 - Pf6N2E2_6020 0.12 -0.0
6,840,450 + Pf6N2E2_6020 0.35 +0.5
6,840,451 - Pf6N2E2_6020 0.35 -1.4
6,840,647 + Pf6N2E2_6020 0.55 +1.6
6,840,647 + Pf6N2E2_6020 0.55 +0.9
6,840,647 + Pf6N2E2_6020 0.55 +1.3
6,840,647 + Pf6N2E2_6020 0.55 -0.6
6,840,647 + Pf6N2E2_6020 0.55 -0.5
6,840,807 - Pf6N2E2_6020 0.72 +0.3
6,841,033 + +0.4
6,841,034 - +1.1
6,841,034 - -0.0
6,841,034 - +1.3
6,841,135 + -0.0
6,841,135 + -0.4
6,841,135 + -0.3
6,841,135 + +0.5
6,841,135 + +0.2
6,841,135 + +1.2
6,841,135 + -0.3
6,841,136 - +0.9
6,841,198 + -0.3
6,841,198 + +0.4
6,841,509 + -0.8
6,841,509 + -0.3
6,841,509 + -0.1
6,841,509 + -0.0
6,841,509 + -0.8
6,841,653 + Pf6N2E2_6022 0.50 -0.4
6,841,653 + Pf6N2E2_6022 0.50 +0.0
6,841,692 + Pf6N2E2_6022 0.62 -0.0
6,841,787 + +1.8
6,841,788 - +1.3
6,841,865 + +1.0
6,841,866 - +0.9
6,841,866 - -1.4
6,841,866 - +2.9
6,841,866 - -1.8
6,841,928 - Pf6N2E2_6023 0.18 +0.2

Or see this region's nucleotide sequence