Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6018 and Pf6N2E2_6019 are separated by 21 nucleotides Pf6N2E2_6019 and Pf6N2E2_6020 are separated by 194 nucleotides Pf6N2E2_6020 and Pf6N2E2_6021 are separated by 144 nucleotides Pf6N2E2_6021 and Pf6N2E2_6022 overlap by 11 nucleotides Pf6N2E2_6022 and Pf6N2E2_6023 are separated by 4 nucleotides
Pf6N2E2_6018 - Fumarylacetoacetate hydrolase family protein, at 6,838,446 to 6,839,144
_6018
Pf6N2E2_6019 - putative oxidoreductase, at 6,839,166 to 6,839,924
_6019
Pf6N2E2_6020 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 6,840,119 to 6,841,075
_6020
Pf6N2E2_6021 - RelB/StbD replicon stabilization protein (antitoxin to RelE/StbE), at 6,841,220 to 6,841,507
_6021
Pf6N2E2_6022 - StbE replicon stabilization toxin, at 6,841,497 to 6,841,808
_6022
Pf6N2E2_6023 - Putative cytoplasmic protein, at 6,841,813 to 6,842,451
_6023
Position (kb)
6840
6841
6842 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6839.139 kb on + strand at 6839.139 kb on + strand at 6839.253 kb on + strand, within Pf6N2E2_6019 at 6839.353 kb on + strand, within Pf6N2E2_6019 at 6839.354 kb on - strand, within Pf6N2E2_6019 at 6839.354 kb on - strand, within Pf6N2E2_6019 at 6839.354 kb on - strand, within Pf6N2E2_6019 at 6839.403 kb on + strand, within Pf6N2E2_6019 at 6839.404 kb on - strand, within Pf6N2E2_6019 at 6839.697 kb on + strand, within Pf6N2E2_6019 at 6839.697 kb on + strand, within Pf6N2E2_6019 at 6839.697 kb on + strand, within Pf6N2E2_6019 at 6839.698 kb on - strand, within Pf6N2E2_6019 at 6839.698 kb on - strand, within Pf6N2E2_6019 at 6839.698 kb on - strand, within Pf6N2E2_6019 at 6839.698 kb on - strand, within Pf6N2E2_6019 at 6839.770 kb on + strand, within Pf6N2E2_6019 at 6839.770 kb on + strand, within Pf6N2E2_6019 at 6839.771 kb on - strand, within Pf6N2E2_6019 at 6839.842 kb on - strand, within Pf6N2E2_6019 at 6840.107 kb on + strand at 6840.230 kb on + strand, within Pf6N2E2_6020 at 6840.230 kb on + strand, within Pf6N2E2_6020 at 6840.230 kb on + strand, within Pf6N2E2_6020 at 6840.231 kb on - strand, within Pf6N2E2_6020 at 6840.231 kb on - strand, within Pf6N2E2_6020 at 6840.231 kb on - strand, within Pf6N2E2_6020 at 6840.231 kb on - strand, within Pf6N2E2_6020 at 6840.231 kb on - strand, within Pf6N2E2_6020 at 6840.450 kb on + strand, within Pf6N2E2_6020 at 6840.451 kb on - strand, within Pf6N2E2_6020 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.807 kb on - strand, within Pf6N2E2_6020 at 6841.033 kb on + strand at 6841.034 kb on - strand at 6841.034 kb on - strand at 6841.034 kb on - strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.136 kb on - strand at 6841.198 kb on + strand at 6841.198 kb on + strand at 6841.509 kb on + strand at 6841.509 kb on + strand at 6841.509 kb on + strand at 6841.509 kb on + strand at 6841.509 kb on + strand at 6841.653 kb on + strand, within Pf6N2E2_6022 at 6841.653 kb on + strand, within Pf6N2E2_6022 at 6841.692 kb on + strand, within Pf6N2E2_6022 at 6841.787 kb on + strand at 6841.788 kb on - strand at 6841.865 kb on + strand at 6841.866 kb on - strand at 6841.866 kb on - strand at 6841.866 kb on - strand at 6841.866 kb on - strand at 6841.928 kb on - strand, within Pf6N2E2_6023
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 6,839,139 + -1.5 6,839,139 + -0.4 6,839,253 + Pf6N2E2_6019 0.11 +0.2 6,839,353 + Pf6N2E2_6019 0.25 +0.5 6,839,354 - Pf6N2E2_6019 0.25 +0.0 6,839,354 - Pf6N2E2_6019 0.25 +0.4 6,839,354 - Pf6N2E2_6019 0.25 +0.7 6,839,403 + Pf6N2E2_6019 0.31 -0.2 6,839,404 - Pf6N2E2_6019 0.31 -0.2 6,839,697 + Pf6N2E2_6019 0.70 -0.3 6,839,697 + Pf6N2E2_6019 0.70 +0.8 6,839,697 + Pf6N2E2_6019 0.70 +0.1 6,839,698 - Pf6N2E2_6019 0.70 -0.8 6,839,698 - Pf6N2E2_6019 0.70 +0.0 6,839,698 - Pf6N2E2_6019 0.70 +1.0 6,839,698 - Pf6N2E2_6019 0.70 -0.4 6,839,770 + Pf6N2E2_6019 0.80 -0.5 6,839,770 + Pf6N2E2_6019 0.80 -0.5 6,839,771 - Pf6N2E2_6019 0.80 -0.1 6,839,842 - Pf6N2E2_6019 0.89 +1.0 6,840,107 + +0.5 6,840,230 + Pf6N2E2_6020 0.12 -0.1 6,840,230 + Pf6N2E2_6020 0.12 -1.5 6,840,230 + Pf6N2E2_6020 0.12 +0.5 6,840,231 - Pf6N2E2_6020 0.12 +1.0 6,840,231 - Pf6N2E2_6020 0.12 -0.1 6,840,231 - Pf6N2E2_6020 0.12 +0.6 6,840,231 - Pf6N2E2_6020 0.12 -0.1 6,840,231 - Pf6N2E2_6020 0.12 -0.0 6,840,450 + Pf6N2E2_6020 0.35 +0.5 6,840,451 - Pf6N2E2_6020 0.35 -1.4 6,840,647 + Pf6N2E2_6020 0.55 +1.6 6,840,647 + Pf6N2E2_6020 0.55 +0.9 6,840,647 + Pf6N2E2_6020 0.55 +1.3 6,840,647 + Pf6N2E2_6020 0.55 -0.6 6,840,647 + Pf6N2E2_6020 0.55 -0.5 6,840,807 - Pf6N2E2_6020 0.72 +0.3 6,841,033 + +0.4 6,841,034 - +1.1 6,841,034 - -0.0 6,841,034 - +1.3 6,841,135 + -0.0 6,841,135 + -0.4 6,841,135 + -0.3 6,841,135 + +0.5 6,841,135 + +0.2 6,841,135 + +1.2 6,841,135 + -0.3 6,841,136 - +0.9 6,841,198 + -0.3 6,841,198 + +0.4 6,841,509 + -0.8 6,841,509 + -0.3 6,841,509 + -0.1 6,841,509 + -0.0 6,841,509 + -0.8 6,841,653 + Pf6N2E2_6022 0.50 -0.4 6,841,653 + Pf6N2E2_6022 0.50 +0.0 6,841,692 + Pf6N2E2_6022 0.62 -0.0 6,841,787 + +1.8 6,841,788 - +1.3 6,841,865 + +1.0 6,841,866 - +0.9 6,841,866 - -1.4 6,841,866 - +2.9 6,841,866 - -1.8 6,841,928 - Pf6N2E2_6023 0.18 +0.2
Or see this region's nucleotide sequence