Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5895 and Pf6N2E2_5896 are separated by 62 nucleotides Pf6N2E2_5896 and Pf6N2E2_5897 overlap by 4 nucleotides
Pf6N2E2_5895 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 6,715,293 to 6,716,549
_5895
Pf6N2E2_5896 - Cystathionine gamma-lyase (EC 4.4.1.1), at 6,716,612 to 6,717,757
_5896
Pf6N2E2_5897 - COG1180: Radical SAM, Pyruvate-formate lyase-activating enzyme like, at 6,717,754 to 6,719,064
_5897
Position (kb)
6716
6717
6718 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6715.648 kb on + strand, within Pf6N2E2_5895 at 6715.780 kb on + strand, within Pf6N2E2_5895 at 6715.780 kb on + strand, within Pf6N2E2_5895 at 6715.780 kb on + strand, within Pf6N2E2_5895 at 6715.780 kb on + strand, within Pf6N2E2_5895 at 6715.780 kb on + strand, within Pf6N2E2_5895 at 6715.882 kb on + strand, within Pf6N2E2_5895 at 6715.883 kb on - strand, within Pf6N2E2_5895 at 6715.891 kb on + strand, within Pf6N2E2_5895 at 6715.892 kb on - strand, within Pf6N2E2_5895 at 6715.892 kb on - strand, within Pf6N2E2_5895 at 6715.892 kb on - strand, within Pf6N2E2_5895 at 6715.985 kb on + strand, within Pf6N2E2_5895 at 6715.985 kb on + strand, within Pf6N2E2_5895 at 6715.986 kb on - strand, within Pf6N2E2_5895 at 6715.994 kb on + strand, within Pf6N2E2_5895 at 6716.163 kb on + strand, within Pf6N2E2_5895 at 6716.164 kb on - strand, within Pf6N2E2_5895 at 6716.169 kb on + strand, within Pf6N2E2_5895 at 6716.169 kb on + strand, within Pf6N2E2_5895 at 6716.169 kb on + strand, within Pf6N2E2_5895 at 6716.169 kb on + strand, within Pf6N2E2_5895 at 6716.169 kb on + strand, within Pf6N2E2_5895 at 6716.169 kb on + strand, within Pf6N2E2_5895 at 6716.170 kb on - strand, within Pf6N2E2_5895 at 6716.170 kb on - strand, within Pf6N2E2_5895 at 6716.170 kb on - strand, within Pf6N2E2_5895 at 6716.383 kb on + strand, within Pf6N2E2_5895 at 6716.487 kb on + strand at 6716.487 kb on + strand at 6716.488 kb on - strand at 6716.488 kb on - strand at 6716.490 kb on + strand at 6716.564 kb on - strand at 6716.871 kb on + strand, within Pf6N2E2_5896 at 6716.871 kb on + strand, within Pf6N2E2_5896 at 6716.872 kb on - strand, within Pf6N2E2_5896 at 6716.872 kb on - strand, within Pf6N2E2_5896 at 6716.896 kb on + strand, within Pf6N2E2_5896 at 6716.955 kb on + strand, within Pf6N2E2_5896 at 6716.955 kb on + strand, within Pf6N2E2_5896 at 6716.956 kb on - strand, within Pf6N2E2_5896 at 6716.956 kb on - strand, within Pf6N2E2_5896 at 6716.956 kb on - strand, within Pf6N2E2_5896 at 6716.956 kb on - strand, within Pf6N2E2_5896 at 6716.956 kb on - strand, within Pf6N2E2_5896 at 6716.956 kb on - strand, within Pf6N2E2_5896 at 6716.988 kb on + strand, within Pf6N2E2_5896 at 6716.988 kb on + strand, within Pf6N2E2_5896 at 6716.989 kb on - strand, within Pf6N2E2_5896 at 6716.989 kb on - strand, within Pf6N2E2_5896 at 6716.989 kb on - strand, within Pf6N2E2_5896 at 6717.086 kb on + strand, within Pf6N2E2_5896 at 6717.099 kb on + strand, within Pf6N2E2_5896 at 6717.100 kb on - strand, within Pf6N2E2_5896 at 6717.186 kb on + strand, within Pf6N2E2_5896 at 6717.186 kb on + strand, within Pf6N2E2_5896 at 6717.187 kb on - strand, within Pf6N2E2_5896 at 6717.201 kb on + strand, within Pf6N2E2_5896 at 6717.202 kb on - strand, within Pf6N2E2_5896 at 6717.259 kb on + strand, within Pf6N2E2_5896 at 6717.260 kb on - strand, within Pf6N2E2_5896 at 6717.470 kb on - strand, within Pf6N2E2_5896 at 6717.470 kb on - strand, within Pf6N2E2_5896 at 6717.470 kb on - strand, within Pf6N2E2_5896 at 6717.471 kb on + strand, within Pf6N2E2_5896 at 6717.483 kb on + strand, within Pf6N2E2_5896 at 6717.484 kb on - strand, within Pf6N2E2_5896 at 6717.484 kb on - strand, within Pf6N2E2_5896 at 6717.484 kb on - strand, within Pf6N2E2_5896 at 6717.490 kb on - strand, within Pf6N2E2_5896 at 6717.540 kb on + strand, within Pf6N2E2_5896 at 6717.540 kb on + strand, within Pf6N2E2_5896 at 6717.540 kb on + strand, within Pf6N2E2_5896 at 6717.540 kb on + strand, within Pf6N2E2_5896 at 6717.540 kb on + strand, within Pf6N2E2_5896 at 6717.540 kb on + strand, within Pf6N2E2_5896 at 6717.540 kb on + strand, within Pf6N2E2_5896 at 6717.541 kb on - strand, within Pf6N2E2_5896 at 6717.541 kb on - strand, within Pf6N2E2_5896 at 6717.541 kb on - strand, within Pf6N2E2_5896 at 6717.564 kb on + strand, within Pf6N2E2_5896 at 6717.565 kb on - strand, within Pf6N2E2_5896 at 6717.645 kb on + strand at 6717.646 kb on - strand at 6717.684 kb on + strand at 6717.688 kb on + strand at 6717.689 kb on - strand at 6717.689 kb on - strand at 6717.730 kb on + strand at 6717.730 kb on + strand at 6717.731 kb on - strand at 6717.731 kb on - strand at 6717.738 kb on - strand at 6717.746 kb on - strand at 6717.788 kb on + strand at 6717.788 kb on + strand at 6717.788 kb on + strand at 6717.788 kb on + strand at 6717.789 kb on - strand at 6717.789 kb on - strand at 6717.789 kb on - strand at 6717.977 kb on + strand, within Pf6N2E2_5897 at 6718.033 kb on - strand, within Pf6N2E2_5897 at 6718.033 kb on - strand, within Pf6N2E2_5897 at 6718.033 kb on - strand, within Pf6N2E2_5897 at 6718.034 kb on + strand, within Pf6N2E2_5897 at 6718.034 kb on + strand, within Pf6N2E2_5897 at 6718.034 kb on + strand, within Pf6N2E2_5897 at 6718.034 kb on + strand, within Pf6N2E2_5897 at 6718.034 kb on + strand at 6718.035 kb on - strand, within Pf6N2E2_5897 at 6718.035 kb on - strand, within Pf6N2E2_5897 at 6718.035 kb on - strand, within Pf6N2E2_5897 at 6718.049 kb on + strand, within Pf6N2E2_5897 at 6718.137 kb on - strand, within Pf6N2E2_5897 at 6718.296 kb on + strand, within Pf6N2E2_5897 at 6718.297 kb on - strand, within Pf6N2E2_5897 at 6718.349 kb on + strand, within Pf6N2E2_5897 at 6718.349 kb on + strand, within Pf6N2E2_5897 at 6718.350 kb on - strand, within Pf6N2E2_5897 at 6718.350 kb on - strand, within Pf6N2E2_5897 at 6718.568 kb on + strand, within Pf6N2E2_5897 at 6718.568 kb on + strand, within Pf6N2E2_5897 at 6718.569 kb on - strand, within Pf6N2E2_5897 at 6718.643 kb on + strand, within Pf6N2E2_5897 at 6718.644 kb on - strand, within Pf6N2E2_5897 at 6718.657 kb on - strand, within Pf6N2E2_5897 at 6718.657 kb on - strand, within Pf6N2E2_5897 at 6718.681 kb on - strand, within Pf6N2E2_5897 at 6718.741 kb on + strand, within Pf6N2E2_5897 at 6718.742 kb on - strand, within Pf6N2E2_5897 at 6718.742 kb on - strand, within Pf6N2E2_5897 at 6718.742 kb on - strand, within Pf6N2E2_5897
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 6,715,648 + Pf6N2E2_5895 0.28 -0.1 6,715,780 + Pf6N2E2_5895 0.39 +0.2 6,715,780 + Pf6N2E2_5895 0.39 +0.2 6,715,780 + Pf6N2E2_5895 0.39 +0.2 6,715,780 + Pf6N2E2_5895 0.39 -0.2 6,715,780 + Pf6N2E2_5895 0.39 +1.2 6,715,882 + Pf6N2E2_5895 0.47 -0.5 6,715,883 - Pf6N2E2_5895 0.47 -2.6 6,715,891 + Pf6N2E2_5895 0.48 +0.3 6,715,892 - Pf6N2E2_5895 0.48 -0.9 6,715,892 - Pf6N2E2_5895 0.48 -1.3 6,715,892 - Pf6N2E2_5895 0.48 -0.8 6,715,985 + Pf6N2E2_5895 0.55 -0.6 6,715,985 + Pf6N2E2_5895 0.55 -0.2 6,715,986 - Pf6N2E2_5895 0.55 -1.0 6,715,994 + Pf6N2E2_5895 0.56 +0.0 6,716,163 + Pf6N2E2_5895 0.69 +0.3 6,716,164 - Pf6N2E2_5895 0.69 +1.9 6,716,169 + Pf6N2E2_5895 0.70 +1.0 6,716,169 + Pf6N2E2_5895 0.70 -3.0 6,716,169 + Pf6N2E2_5895 0.70 +0.2 6,716,169 + Pf6N2E2_5895 0.70 -1.3 6,716,169 + Pf6N2E2_5895 0.70 -0.1 6,716,169 + Pf6N2E2_5895 0.70 +0.4 6,716,170 - Pf6N2E2_5895 0.70 +0.3 6,716,170 - Pf6N2E2_5895 0.70 +0.1 6,716,170 - Pf6N2E2_5895 0.70 -1.1 6,716,383 + Pf6N2E2_5895 0.87 -0.3 6,716,487 + -1.4 6,716,487 + -0.4 6,716,488 - -0.7 6,716,488 - -0.1 6,716,490 + +0.4 6,716,564 - -0.6 6,716,871 + Pf6N2E2_5896 0.23 -0.1 6,716,871 + Pf6N2E2_5896 0.23 +1.0 6,716,872 - Pf6N2E2_5896 0.23 +0.6 6,716,872 - Pf6N2E2_5896 0.23 +0.5 6,716,896 + Pf6N2E2_5896 0.25 +0.5 6,716,955 + Pf6N2E2_5896 0.30 +0.0 6,716,955 + Pf6N2E2_5896 0.30 -0.5 6,716,956 - Pf6N2E2_5896 0.30 +0.1 6,716,956 - Pf6N2E2_5896 0.30 +1.0 6,716,956 - Pf6N2E2_5896 0.30 +1.6 6,716,956 - Pf6N2E2_5896 0.30 +0.7 6,716,956 - Pf6N2E2_5896 0.30 -0.3 6,716,956 - Pf6N2E2_5896 0.30 +0.0 6,716,988 + Pf6N2E2_5896 0.33 -1.4 6,716,988 + Pf6N2E2_5896 0.33 -1.1 6,716,989 - Pf6N2E2_5896 0.33 +0.2 6,716,989 - Pf6N2E2_5896 0.33 +0.4 6,716,989 - Pf6N2E2_5896 0.33 +0.3 6,717,086 + Pf6N2E2_5896 0.41 +0.4 6,717,099 + Pf6N2E2_5896 0.42 +0.5 6,717,100 - Pf6N2E2_5896 0.43 -1.2 6,717,186 + Pf6N2E2_5896 0.50 +0.3 6,717,186 + Pf6N2E2_5896 0.50 +0.1 6,717,187 - Pf6N2E2_5896 0.50 +0.8 6,717,201 + Pf6N2E2_5896 0.51 +0.2 6,717,202 - Pf6N2E2_5896 0.51 -1.3 6,717,259 + Pf6N2E2_5896 0.56 -1.8 6,717,260 - Pf6N2E2_5896 0.57 +0.3 6,717,470 - Pf6N2E2_5896 0.75 -2.0 6,717,470 - Pf6N2E2_5896 0.75 +1.1 6,717,470 - Pf6N2E2_5896 0.75 -0.0 6,717,471 + Pf6N2E2_5896 0.75 -1.6 6,717,483 + Pf6N2E2_5896 0.76 -0.6 6,717,484 - Pf6N2E2_5896 0.76 -0.1 6,717,484 - Pf6N2E2_5896 0.76 +0.6 6,717,484 - Pf6N2E2_5896 0.76 +1.0 6,717,490 - Pf6N2E2_5896 0.77 -0.1 6,717,540 + Pf6N2E2_5896 0.81 +0.8 6,717,540 + Pf6N2E2_5896 0.81 -0.1 6,717,540 + Pf6N2E2_5896 0.81 -1.8 6,717,540 + Pf6N2E2_5896 0.81 +0.4 6,717,540 + Pf6N2E2_5896 0.81 +1.4 6,717,540 + Pf6N2E2_5896 0.81 +0.0 6,717,540 + Pf6N2E2_5896 0.81 -0.6 6,717,541 - Pf6N2E2_5896 0.81 +0.7 6,717,541 - Pf6N2E2_5896 0.81 -1.8 6,717,541 - Pf6N2E2_5896 0.81 -0.3 6,717,564 + Pf6N2E2_5896 0.83 +0.1 6,717,565 - Pf6N2E2_5896 0.83 +0.2 6,717,645 + +0.4 6,717,646 - +0.6 6,717,684 + -0.3 6,717,688 + -1.3 6,717,689 - -0.1 6,717,689 - +0.5 6,717,730 + -0.6 6,717,730 + -0.7 6,717,731 - +0.8 6,717,731 - +0.6 6,717,738 - -1.4 6,717,746 - -0.8 6,717,788 + -0.0 6,717,788 + -0.7 6,717,788 + +2.5 6,717,788 + +0.7 6,717,789 - -1.2 6,717,789 - -0.3 6,717,789 - -1.9 6,717,977 + Pf6N2E2_5897 0.17 +0.9 6,718,033 - Pf6N2E2_5897 0.21 -0.7 6,718,033 - Pf6N2E2_5897 0.21 -0.4 6,718,033 - Pf6N2E2_5897 0.21 +0.9 6,718,034 + Pf6N2E2_5897 0.21 -0.0 6,718,034 + Pf6N2E2_5897 0.21 -0.5 6,718,034 + Pf6N2E2_5897 0.21 -0.3 6,718,034 + Pf6N2E2_5897 0.21 -0.3 6,718,034 + +0.1 6,718,035 - Pf6N2E2_5897 0.21 +0.3 6,718,035 - Pf6N2E2_5897 0.21 +0.5 6,718,035 - Pf6N2E2_5897 0.21 -0.5 6,718,049 + Pf6N2E2_5897 0.23 -0.5 6,718,137 - Pf6N2E2_5897 0.29 +0.5 6,718,296 + Pf6N2E2_5897 0.41 +1.1 6,718,297 - Pf6N2E2_5897 0.41 +0.2 6,718,349 + Pf6N2E2_5897 0.45 -1.0 6,718,349 + Pf6N2E2_5897 0.45 +0.8 6,718,350 - Pf6N2E2_5897 0.45 -1.2 6,718,350 - Pf6N2E2_5897 0.45 -0.6 6,718,568 + Pf6N2E2_5897 0.62 +0.2 6,718,568 + Pf6N2E2_5897 0.62 +0.5 6,718,569 - Pf6N2E2_5897 0.62 +0.2 6,718,643 + Pf6N2E2_5897 0.68 +0.4 6,718,644 - Pf6N2E2_5897 0.68 -0.1 6,718,657 - Pf6N2E2_5897 0.69 -1.2 6,718,657 - Pf6N2E2_5897 0.69 -0.1 6,718,681 - Pf6N2E2_5897 0.71 -0.7 6,718,741 + Pf6N2E2_5897 0.75 +0.8 6,718,742 - Pf6N2E2_5897 0.75 +0.5 6,718,742 - Pf6N2E2_5897 0.75 -0.3 6,718,742 - Pf6N2E2_5897 0.75 -1.5
Or see this region's nucleotide sequence