Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5701 and Pf6N2E2_5702 are separated by 11 nucleotides Pf6N2E2_5702 and Pf6N2E2_5703 are separated by 147 nucleotides Pf6N2E2_5703 and Pf6N2E2_5704 are separated by 104 nucleotides Pf6N2E2_5704 and Pf6N2E2_5705 overlap by 4 nucleotides
Pf6N2E2_5701 - 4'-phosphopantetheinyl transferase entD (EC 2.7.8.-); Holo-[acyl-carrier protein] synthase, alternative (EC 2.7.8.7), at 6,517,125 to 6,518,060
_5701
Pf6N2E2_5702 - Dienelactone hydrolase family, at 6,518,072 to 6,518,863
_5702
Pf6N2E2_5703 - outer membrane protein H1, at 6,519,011 to 6,519,610
_5703
Pf6N2E2_5704 - Transcriptional regulatory protein PhoP, at 6,519,715 to 6,520,392
_5704
Pf6N2E2_5705 - Sensor protein PhoQ (EC 2.7.13.3), at 6,520,389 to 6,521,735
_5705
Position (kb)
6519
6520 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 6518.104 kb on + strand at 6518.139 kb on + strand at 6518.139 kb on + strand at 6518.140 kb on - strand at 6518.234 kb on - strand, within Pf6N2E2_5702 at 6518.234 kb on - strand, within Pf6N2E2_5702 at 6518.234 kb on - strand, within Pf6N2E2_5702 at 6518.236 kb on - strand, within Pf6N2E2_5702 at 6518.430 kb on - strand, within Pf6N2E2_5702 at 6518.501 kb on + strand, within Pf6N2E2_5702 at 6518.502 kb on - strand, within Pf6N2E2_5702 at 6518.610 kb on + strand, within Pf6N2E2_5702 at 6518.610 kb on + strand, within Pf6N2E2_5702 at 6518.610 kb on + strand, within Pf6N2E2_5702 at 6518.610 kb on + strand, within Pf6N2E2_5702 at 6518.610 kb on + strand, within Pf6N2E2_5702 at 6518.610 kb on + strand, within Pf6N2E2_5702 at 6518.610 kb on + strand, within Pf6N2E2_5702 at 6518.611 kb on - strand, within Pf6N2E2_5702 at 6518.611 kb on - strand, within Pf6N2E2_5702 at 6518.611 kb on - strand, within Pf6N2E2_5702 at 6518.635 kb on - strand, within Pf6N2E2_5702 at 6518.635 kb on - strand, within Pf6N2E2_5702 at 6518.674 kb on - strand, within Pf6N2E2_5702 at 6518.742 kb on + strand, within Pf6N2E2_5702 at 6518.742 kb on + strand, within Pf6N2E2_5702 at 6518.748 kb on + strand, within Pf6N2E2_5702 at 6518.748 kb on + strand, within Pf6N2E2_5702 at 6518.748 kb on + strand, within Pf6N2E2_5702 at 6518.748 kb on + strand, within Pf6N2E2_5702 at 6518.749 kb on - strand, within Pf6N2E2_5702 at 6518.749 kb on - strand, within Pf6N2E2_5702 at 6518.751 kb on + strand, within Pf6N2E2_5702 at 6518.784 kb on + strand at 6518.840 kb on + strand at 6518.840 kb on + strand at 6518.841 kb on - strand at 6518.841 kb on - strand at 6518.841 kb on - strand at 6518.841 kb on - strand at 6518.841 kb on - strand at 6518.902 kb on - strand at 6518.902 kb on - strand at 6519.197 kb on + strand, within Pf6N2E2_5703 at 6519.198 kb on - strand, within Pf6N2E2_5703 at 6519.236 kb on + strand, within Pf6N2E2_5703 at 6519.249 kb on - strand, within Pf6N2E2_5703 at 6519.311 kb on + strand, within Pf6N2E2_5703 at 6519.311 kb on + strand, within Pf6N2E2_5703 at 6519.311 kb on + strand, within Pf6N2E2_5703 at 6519.311 kb on + strand, within Pf6N2E2_5703 at 6519.312 kb on - strand, within Pf6N2E2_5703 at 6519.491 kb on + strand at 6519.584 kb on + strand at 6519.584 kb on + strand at 6519.584 kb on + strand at 6519.600 kb on - strand at 6519.629 kb on - strand at 6520.006 kb on + strand, within Pf6N2E2_5704 at 6520.006 kb on + strand, within Pf6N2E2_5704 at 6520.006 kb on + strand, within Pf6N2E2_5704 at 6520.006 kb on + strand, within Pf6N2E2_5704 at 6520.007 kb on - strand, within Pf6N2E2_5704 at 6520.007 kb on - strand, within Pf6N2E2_5704 at 6520.007 kb on - strand, within Pf6N2E2_5704 at 6520.081 kb on - strand, within Pf6N2E2_5704 at 6520.172 kb on - strand, within Pf6N2E2_5704 at 6520.177 kb on + strand, within Pf6N2E2_5704 at 6520.193 kb on - strand, within Pf6N2E2_5704
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 6,518,104 + +0.7 6,518,139 + -0.2 6,518,139 + +0.4 6,518,140 - +0.0 6,518,234 - Pf6N2E2_5702 0.20 +0.0 6,518,234 - Pf6N2E2_5702 0.20 -0.8 6,518,234 - Pf6N2E2_5702 0.20 +2.1 6,518,236 - Pf6N2E2_5702 0.21 +0.4 6,518,430 - Pf6N2E2_5702 0.45 +0.4 6,518,501 + Pf6N2E2_5702 0.54 -0.7 6,518,502 - Pf6N2E2_5702 0.54 -0.8 6,518,610 + Pf6N2E2_5702 0.68 +0.0 6,518,610 + Pf6N2E2_5702 0.68 -0.4 6,518,610 + Pf6N2E2_5702 0.68 +0.4 6,518,610 + Pf6N2E2_5702 0.68 +0.5 6,518,610 + Pf6N2E2_5702 0.68 +0.0 6,518,610 + Pf6N2E2_5702 0.68 +0.4 6,518,610 + Pf6N2E2_5702 0.68 +0.6 6,518,611 - Pf6N2E2_5702 0.68 +0.3 6,518,611 - Pf6N2E2_5702 0.68 +0.2 6,518,611 - Pf6N2E2_5702 0.68 -0.5 6,518,635 - Pf6N2E2_5702 0.71 +0.7 6,518,635 - Pf6N2E2_5702 0.71 +0.1 6,518,674 - Pf6N2E2_5702 0.76 -1.1 6,518,742 + Pf6N2E2_5702 0.85 -0.6 6,518,742 + Pf6N2E2_5702 0.85 +0.6 6,518,748 + Pf6N2E2_5702 0.85 -0.1 6,518,748 + Pf6N2E2_5702 0.85 -0.0 6,518,748 + Pf6N2E2_5702 0.85 -0.1 6,518,748 + Pf6N2E2_5702 0.85 +1.1 6,518,749 - Pf6N2E2_5702 0.85 +0.3 6,518,749 - Pf6N2E2_5702 0.85 +0.3 6,518,751 + Pf6N2E2_5702 0.86 -1.4 6,518,784 + +0.2 6,518,840 + -0.8 6,518,840 + -0.5 6,518,841 - +1.0 6,518,841 - +0.8 6,518,841 - -2.1 6,518,841 - +0.5 6,518,841 - +0.6 6,518,902 - +1.1 6,518,902 - +0.5 6,519,197 + Pf6N2E2_5703 0.31 -2.4 6,519,198 - Pf6N2E2_5703 0.31 -3.5 6,519,236 + Pf6N2E2_5703 0.38 -0.5 6,519,249 - Pf6N2E2_5703 0.40 -2.8 6,519,311 + Pf6N2E2_5703 0.50 -1.8 6,519,311 + Pf6N2E2_5703 0.50 -1.9 6,519,311 + Pf6N2E2_5703 0.50 -2.1 6,519,311 + Pf6N2E2_5703 0.50 -2.3 6,519,312 - Pf6N2E2_5703 0.50 -2.3 6,519,491 + +0.3 6,519,584 + -3.0 6,519,584 + -2.4 6,519,584 + -1.4 6,519,600 - -1.1 6,519,629 - -1.3 6,520,006 + Pf6N2E2_5704 0.43 -1.7 6,520,006 + Pf6N2E2_5704 0.43 -2.0 6,520,006 + Pf6N2E2_5704 0.43 -3.0 6,520,006 + Pf6N2E2_5704 0.43 -3.6 6,520,007 - Pf6N2E2_5704 0.43 -1.4 6,520,007 - Pf6N2E2_5704 0.43 -4.0 6,520,007 - Pf6N2E2_5704 0.43 -3.3 6,520,081 - Pf6N2E2_5704 0.54 -3.8 6,520,172 - Pf6N2E2_5704 0.67 -0.8 6,520,177 + Pf6N2E2_5704 0.68 -4.1 6,520,193 - Pf6N2E2_5704 0.71 -0.7
Or see this region's nucleotide sequence