Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_568 and Pf6N2E2_569 are separated by 197 nucleotides Pf6N2E2_569 and Pf6N2E2_570 are separated by 127 nucleotides Pf6N2E2_570 and Pf6N2E2_571 are separated by 23 nucleotides
Pf6N2E2_568 - tRNA-(ms[2]io[6]A)-hydroxylase (EC 1.-.-.-), at 643,266 to 643,871
_568
Pf6N2E2_569 - FIG00793915: hypothetical protein, at 644,069 to 644,557
_569
Pf6N2E2_570 - DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 644,685 to 645,302
_570
Pf6N2E2_571 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 645,326 to 646,369
_571
Position (kb) 644
645
646 Strain fitness (log2 ratio) -1
0
1
2 at 643.693 kb on - strand, within Pf6N2E2_568 at 643.734 kb on + strand, within Pf6N2E2_568 at 643.734 kb on + strand, within Pf6N2E2_568 at 643.870 kb on - strand at 643.882 kb on + strand at 643.883 kb on - strand at 643.895 kb on + strand at 643.921 kb on + strand at 643.921 kb on + strand at 643.921 kb on + strand at 643.921 kb on + strand at 643.922 kb on - strand at 643.947 kb on + strand at 643.947 kb on + strand at 644.053 kb on - strand at 644.070 kb on + strand at 644.070 kb on + strand at 644.070 kb on + strand at 644.581 kb on - strand at 644.581 kb on - strand at 644.711 kb on - strand at 644.711 kb on - strand at 644.864 kb on - strand, within Pf6N2E2_570 at 644.864 kb on - strand, within Pf6N2E2_570 at 644.871 kb on + strand, within Pf6N2E2_570 at 645.157 kb on + strand, within Pf6N2E2_570 at 645.158 kb on - strand, within Pf6N2E2_570 at 645.307 kb on + strand at 645.308 kb on - strand at 645.374 kb on + strand at 645.721 kb on - strand, within Pf6N2E2_571 at 645.725 kb on + strand, within Pf6N2E2_571 at 645.726 kb on - strand, within Pf6N2E2_571 at 645.726 kb on - strand, within Pf6N2E2_571 at 645.726 kb on - strand, within Pf6N2E2_571 at 645.726 kb on - strand, within Pf6N2E2_571 at 645.726 kb on - strand, within Pf6N2E2_571 at 646.015 kb on - strand, within Pf6N2E2_571 at 646.015 kb on - strand, within Pf6N2E2_571 at 646.015 kb on - strand, within Pf6N2E2_571 at 646.298 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 643,693 - Pf6N2E2_568 0.70 +0.2 643,734 + Pf6N2E2_568 0.77 -0.4 643,734 + Pf6N2E2_568 0.77 -0.7 643,870 - +0.1 643,882 + +0.5 643,883 - -0.2 643,895 + +1.0 643,921 + -0.9 643,921 + -0.5 643,921 + +1.0 643,921 + +0.1 643,922 - +0.2 643,947 + -0.3 643,947 + +0.2 644,053 - -1.0 644,070 + -0.9 644,070 + +0.6 644,070 + -0.2 644,581 - +1.3 644,581 - +0.4 644,711 - +0.9 644,711 - +0.8 644,864 - Pf6N2E2_570 0.29 -0.8 644,864 - Pf6N2E2_570 0.29 +0.2 644,871 + Pf6N2E2_570 0.30 +0.8 645,157 + Pf6N2E2_570 0.76 +1.5 645,158 - Pf6N2E2_570 0.77 -0.5 645,307 + +2.1 645,308 - -0.1 645,374 + +0.1 645,721 - Pf6N2E2_571 0.38 +1.3 645,725 + Pf6N2E2_571 0.38 -0.8 645,726 - Pf6N2E2_571 0.38 +0.7 645,726 - Pf6N2E2_571 0.38 +1.5 645,726 - Pf6N2E2_571 0.38 +0.3 645,726 - Pf6N2E2_571 0.38 -0.6 645,726 - Pf6N2E2_571 0.38 +1.3 646,015 - Pf6N2E2_571 0.66 -0.7 646,015 - Pf6N2E2_571 0.66 +0.4 646,015 - Pf6N2E2_571 0.66 +1.6 646,298 - +0.3
Or see this region's nucleotide sequence