Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5631 and Pf6N2E2_5632 are separated by 745 nucleotides Pf6N2E2_5632 and Pf6N2E2_5633 are separated by 34 nucleotides Pf6N2E2_5633 and Pf6N2E2_5634 are separated by 338 nucleotides Pf6N2E2_5634 and Pf6N2E2_5635 overlap by 4 nucleotides
Pf6N2E2_5631 - hypothetical protein, at 6,446,846 to 6,446,983
_5631
Pf6N2E2_5632 - Aromatic amino acid transport protein AroP, at 6,447,729 to 6,447,887
_5632
Pf6N2E2_5633 - Aromatic amino acid transport protein AroP, at 6,447,922 to 6,449,340
_5633
Pf6N2E2_5634 - Hypothetical protein YaeJ with similarity to translation release factor, at 6,449,679 to 6,450,092
_5634
Pf6N2E2_5635 - Xanthine transporter,putative, at 6,450,089 to 6,451,288
_5635
Position (kb)
6447
6448
6449
6450 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 6446.984 kb on - strand at 6446.984 kb on - strand at 6446.995 kb on + strand at 6446.995 kb on + strand at 6446.995 kb on + strand at 6446.996 kb on - strand at 6446.996 kb on - strand at 6447.014 kb on + strand at 6447.103 kb on - strand at 6447.103 kb on - strand at 6447.103 kb on - strand at 6447.183 kb on + strand at 6447.184 kb on - strand at 6447.221 kb on + strand at 6447.225 kb on + strand at 6447.225 kb on + strand at 6447.225 kb on + strand at 6447.225 kb on + strand at 6447.226 kb on - strand at 6447.226 kb on - strand at 6447.226 kb on - strand at 6447.229 kb on + strand at 6447.229 kb on + strand at 6447.230 kb on - strand at 6447.230 kb on - strand at 6447.356 kb on + strand at 6447.469 kb on - strand at 6447.469 kb on - strand at 6447.469 kb on - strand at 6447.667 kb on + strand at 6447.667 kb on + strand at 6447.668 kb on - strand at 6447.668 kb on - strand at 6447.668 kb on - strand at 6447.780 kb on - strand, within Pf6N2E2_5632 at 6447.780 kb on - strand, within Pf6N2E2_5632 at 6447.780 kb on - strand, within Pf6N2E2_5632 at 6447.780 kb on - strand, within Pf6N2E2_5632 at 6447.809 kb on + strand, within Pf6N2E2_5632 at 6447.809 kb on + strand, within Pf6N2E2_5632 at 6447.809 kb on + strand, within Pf6N2E2_5632 at 6448.177 kb on + strand, within Pf6N2E2_5633 at 6448.177 kb on + strand, within Pf6N2E2_5633 at 6448.177 kb on + strand, within Pf6N2E2_5633 at 6448.178 kb on - strand, within Pf6N2E2_5633 at 6448.178 kb on - strand, within Pf6N2E2_5633 at 6448.228 kb on + strand, within Pf6N2E2_5633 at 6448.228 kb on + strand, within Pf6N2E2_5633 at 6448.228 kb on + strand, within Pf6N2E2_5633 at 6448.229 kb on - strand, within Pf6N2E2_5633 at 6448.229 kb on - strand, within Pf6N2E2_5633 at 6448.270 kb on + strand, within Pf6N2E2_5633 at 6448.270 kb on + strand, within Pf6N2E2_5633 at 6448.270 kb on + strand, within Pf6N2E2_5633 at 6448.270 kb on + strand, within Pf6N2E2_5633 at 6448.271 kb on - strand, within Pf6N2E2_5633 at 6448.271 kb on - strand, within Pf6N2E2_5633 at 6448.271 kb on - strand, within Pf6N2E2_5633 at 6448.271 kb on - strand, within Pf6N2E2_5633 at 6448.271 kb on - strand, within Pf6N2E2_5633 at 6448.271 kb on - strand, within Pf6N2E2_5633 at 6448.271 kb on - strand, within Pf6N2E2_5633 at 6448.271 kb on - strand, within Pf6N2E2_5633 at 6448.279 kb on + strand, within Pf6N2E2_5633 at 6448.280 kb on - strand, within Pf6N2E2_5633 at 6448.438 kb on + strand, within Pf6N2E2_5633 at 6448.438 kb on + strand, within Pf6N2E2_5633 at 6448.438 kb on + strand, within Pf6N2E2_5633 at 6448.438 kb on + strand, within Pf6N2E2_5633 at 6448.439 kb on - strand, within Pf6N2E2_5633 at 6448.439 kb on - strand, within Pf6N2E2_5633 at 6448.439 kb on - strand, within Pf6N2E2_5633 at 6448.439 kb on - strand, within Pf6N2E2_5633 at 6448.648 kb on + strand, within Pf6N2E2_5633 at 6448.649 kb on - strand, within Pf6N2E2_5633 at 6448.667 kb on - strand, within Pf6N2E2_5633 at 6448.747 kb on + strand, within Pf6N2E2_5633 at 6448.748 kb on - strand, within Pf6N2E2_5633 at 6448.748 kb on - strand, within Pf6N2E2_5633 at 6448.806 kb on + strand, within Pf6N2E2_5633 at 6448.807 kb on - strand, within Pf6N2E2_5633 at 6448.846 kb on + strand, within Pf6N2E2_5633 at 6448.846 kb on + strand, within Pf6N2E2_5633 at 6448.847 kb on - strand, within Pf6N2E2_5633 at 6448.861 kb on + strand, within Pf6N2E2_5633 at 6448.861 kb on + strand, within Pf6N2E2_5633 at 6448.861 kb on + strand, within Pf6N2E2_5633 at 6448.861 kb on + strand, within Pf6N2E2_5633 at 6448.861 kb on + strand, within Pf6N2E2_5633 at 6448.861 kb on + strand, within Pf6N2E2_5633 at 6448.861 kb on + strand, within Pf6N2E2_5633 at 6448.862 kb on - strand, within Pf6N2E2_5633 at 6448.862 kb on - strand, within Pf6N2E2_5633 at 6448.862 kb on - strand, within Pf6N2E2_5633 at 6448.862 kb on - strand, within Pf6N2E2_5633 at 6448.875 kb on + strand, within Pf6N2E2_5633 at 6448.875 kb on + strand, within Pf6N2E2_5633 at 6448.875 kb on + strand, within Pf6N2E2_5633 at 6448.875 kb on + strand, within Pf6N2E2_5633 at 6448.876 kb on - strand, within Pf6N2E2_5633 at 6448.876 kb on - strand, within Pf6N2E2_5633 at 6448.876 kb on - strand, within Pf6N2E2_5633 at 6448.876 kb on - strand, within Pf6N2E2_5633 at 6448.876 kb on - strand, within Pf6N2E2_5633 at 6448.876 kb on - strand, within Pf6N2E2_5633 at 6448.876 kb on - strand, within Pf6N2E2_5633 at 6448.876 kb on - strand, within Pf6N2E2_5633 at 6448.876 kb on - strand, within Pf6N2E2_5633 at 6449.002 kb on + strand, within Pf6N2E2_5633 at 6449.002 kb on + strand, within Pf6N2E2_5633 at 6449.002 kb on + strand, within Pf6N2E2_5633 at 6449.002 kb on + strand, within Pf6N2E2_5633 at 6449.002 kb on + strand, within Pf6N2E2_5633 at 6449.083 kb on + strand, within Pf6N2E2_5633 at 6449.083 kb on + strand, within Pf6N2E2_5633 at 6449.167 kb on + strand, within Pf6N2E2_5633 at 6449.167 kb on + strand, within Pf6N2E2_5633 at 6449.167 kb on + strand, within Pf6N2E2_5633 at 6449.167 kb on + strand, within Pf6N2E2_5633 at 6449.167 kb on + strand, within Pf6N2E2_5633 at 6449.167 kb on + strand, within Pf6N2E2_5633 at 6449.167 kb on + strand, within Pf6N2E2_5633 at 6449.167 kb on + strand, within Pf6N2E2_5633 at 6449.168 kb on - strand, within Pf6N2E2_5633 at 6449.168 kb on - strand, within Pf6N2E2_5633 at 6449.168 kb on - strand, within Pf6N2E2_5633 at 6449.168 kb on - strand, within Pf6N2E2_5633 at 6449.168 kb on - strand, within Pf6N2E2_5633 at 6449.338 kb on + strand at 6449.355 kb on - strand at 6449.355 kb on - strand at 6449.378 kb on + strand at 6449.482 kb on + strand at 6449.483 kb on - strand at 6449.483 kb on - strand at 6449.574 kb on - strand at 6449.832 kb on + strand, within Pf6N2E2_5634 at 6449.832 kb on + strand, within Pf6N2E2_5634 at 6449.833 kb on - strand, within Pf6N2E2_5634 at 6449.833 kb on - strand, within Pf6N2E2_5634 at 6449.833 kb on - strand, within Pf6N2E2_5634 at 6449.833 kb on - strand, within Pf6N2E2_5634 at 6449.871 kb on - strand, within Pf6N2E2_5634 at 6449.871 kb on - strand, within Pf6N2E2_5634 at 6449.884 kb on + strand, within Pf6N2E2_5634 at 6449.884 kb on + strand, within Pf6N2E2_5634 at 6449.885 kb on - strand, within Pf6N2E2_5634 at 6449.885 kb on - strand, within Pf6N2E2_5634 at 6449.885 kb on - strand, within Pf6N2E2_5634 at 6449.885 kb on - strand, within Pf6N2E2_5634 at 6450.091 kb on - strand at 6450.091 kb on - strand at 6450.091 kb on - strand at 6450.133 kb on - strand at 6450.138 kb on + strand at 6450.138 kb on + strand at 6450.138 kb on + strand at 6450.138 kb on + strand at 6450.138 kb on + strand at 6450.138 kb on + strand at 6450.138 kb on + strand at 6450.139 kb on - strand at 6450.270 kb on + strand, within Pf6N2E2_5635 at 6450.271 kb on - strand, within Pf6N2E2_5635
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 6,446,984 - +0.3 6,446,984 - -0.8 6,446,995 + -2.5 6,446,995 + -2.6 6,446,995 + -0.5 6,446,996 - +0.8 6,446,996 - -0.4 6,447,014 + -1.0 6,447,103 - +0.7 6,447,103 - -0.0 6,447,103 - +0.3 6,447,183 + +0.1 6,447,184 - +0.6 6,447,221 + -0.8 6,447,225 + +1.2 6,447,225 + -0.4 6,447,225 + +0.1 6,447,225 + -0.5 6,447,226 - +0.3 6,447,226 - -1.0 6,447,226 - +0.4 6,447,229 + +1.9 6,447,229 + +0.7 6,447,230 - +1.7 6,447,230 - -0.9 6,447,356 + -1.1 6,447,469 - +0.8 6,447,469 - +0.3 6,447,469 - +0.8 6,447,667 + +0.1 6,447,667 + +1.2 6,447,668 - +1.7 6,447,668 - -0.8 6,447,668 - -0.9 6,447,780 - Pf6N2E2_5632 0.32 -1.7 6,447,780 - Pf6N2E2_5632 0.32 -1.3 6,447,780 - Pf6N2E2_5632 0.32 -3.4 6,447,780 - Pf6N2E2_5632 0.32 -3.9 6,447,809 + Pf6N2E2_5632 0.50 -0.5 6,447,809 + Pf6N2E2_5632 0.50 -0.0 6,447,809 + Pf6N2E2_5632 0.50 -0.1 6,448,177 + Pf6N2E2_5633 0.18 -3.2 6,448,177 + Pf6N2E2_5633 0.18 -2.8 6,448,177 + Pf6N2E2_5633 0.18 -2.8 6,448,178 - Pf6N2E2_5633 0.18 -0.8 6,448,178 - Pf6N2E2_5633 0.18 -1.3 6,448,228 + Pf6N2E2_5633 0.22 -4.0 6,448,228 + Pf6N2E2_5633 0.22 -3.0 6,448,228 + Pf6N2E2_5633 0.22 -2.9 6,448,229 - Pf6N2E2_5633 0.22 -1.0 6,448,229 - Pf6N2E2_5633 0.22 -1.0 6,448,270 + Pf6N2E2_5633 0.25 -2.8 6,448,270 + Pf6N2E2_5633 0.25 -1.3 6,448,270 + Pf6N2E2_5633 0.25 -0.9 6,448,270 + Pf6N2E2_5633 0.25 -3.2 6,448,271 - Pf6N2E2_5633 0.25 -0.0 6,448,271 - Pf6N2E2_5633 0.25 -1.0 6,448,271 - Pf6N2E2_5633 0.25 -2.2 6,448,271 - Pf6N2E2_5633 0.25 -2.3 6,448,271 - Pf6N2E2_5633 0.25 -1.7 6,448,271 - Pf6N2E2_5633 0.25 -1.7 6,448,271 - Pf6N2E2_5633 0.25 -1.2 6,448,271 - Pf6N2E2_5633 0.25 -3.8 6,448,279 + Pf6N2E2_5633 0.25 -1.2 6,448,280 - Pf6N2E2_5633 0.25 +0.2 6,448,438 + Pf6N2E2_5633 0.36 -0.2 6,448,438 + Pf6N2E2_5633 0.36 -1.2 6,448,438 + Pf6N2E2_5633 0.36 -1.3 6,448,438 + Pf6N2E2_5633 0.36 -3.7 6,448,439 - Pf6N2E2_5633 0.36 -0.3 6,448,439 - Pf6N2E2_5633 0.36 -2.1 6,448,439 - Pf6N2E2_5633 0.36 -2.8 6,448,439 - Pf6N2E2_5633 0.36 -2.2 6,448,648 + Pf6N2E2_5633 0.51 -1.5 6,448,649 - Pf6N2E2_5633 0.51 -2.0 6,448,667 - Pf6N2E2_5633 0.53 -0.1 6,448,747 + Pf6N2E2_5633 0.58 -0.6 6,448,748 - Pf6N2E2_5633 0.58 -3.1 6,448,748 - Pf6N2E2_5633 0.58 -0.6 6,448,806 + Pf6N2E2_5633 0.62 -2.1 6,448,807 - Pf6N2E2_5633 0.62 -2.3 6,448,846 + Pf6N2E2_5633 0.65 -1.3 6,448,846 + Pf6N2E2_5633 0.65 -3.8 6,448,847 - Pf6N2E2_5633 0.65 -3.0 6,448,861 + Pf6N2E2_5633 0.66 -1.3 6,448,861 + Pf6N2E2_5633 0.66 -2.2 6,448,861 + Pf6N2E2_5633 0.66 -1.7 6,448,861 + Pf6N2E2_5633 0.66 -2.2 6,448,861 + Pf6N2E2_5633 0.66 -3.6 6,448,861 + Pf6N2E2_5633 0.66 -0.8 6,448,861 + Pf6N2E2_5633 0.66 -0.9 6,448,862 - Pf6N2E2_5633 0.66 -1.2 6,448,862 - Pf6N2E2_5633 0.66 -2.0 6,448,862 - Pf6N2E2_5633 0.66 -2.3 6,448,862 - Pf6N2E2_5633 0.66 -1.4 6,448,875 + Pf6N2E2_5633 0.67 -2.3 6,448,875 + Pf6N2E2_5633 0.67 -2.6 6,448,875 + Pf6N2E2_5633 0.67 -2.5 6,448,875 + Pf6N2E2_5633 0.67 -1.7 6,448,876 - Pf6N2E2_5633 0.67 -2.1 6,448,876 - Pf6N2E2_5633 0.67 -2.2 6,448,876 - Pf6N2E2_5633 0.67 +0.1 6,448,876 - Pf6N2E2_5633 0.67 -2.2 6,448,876 - Pf6N2E2_5633 0.67 -1.5 6,448,876 - Pf6N2E2_5633 0.67 -0.8 6,448,876 - Pf6N2E2_5633 0.67 -2.6 6,448,876 - Pf6N2E2_5633 0.67 -2.2 6,448,876 - Pf6N2E2_5633 0.67 -1.0 6,449,002 + Pf6N2E2_5633 0.76 -1.5 6,449,002 + Pf6N2E2_5633 0.76 -1.5 6,449,002 + Pf6N2E2_5633 0.76 -0.3 6,449,002 + Pf6N2E2_5633 0.76 -3.9 6,449,002 + Pf6N2E2_5633 0.76 -1.8 6,449,083 + Pf6N2E2_5633 0.82 +0.2 6,449,083 + Pf6N2E2_5633 0.82 -1.6 6,449,167 + Pf6N2E2_5633 0.88 -1.9 6,449,167 + Pf6N2E2_5633 0.88 -2.0 6,449,167 + Pf6N2E2_5633 0.88 -1.7 6,449,167 + Pf6N2E2_5633 0.88 -1.1 6,449,167 + Pf6N2E2_5633 0.88 -1.5 6,449,167 + Pf6N2E2_5633 0.88 -2.1 6,449,167 + Pf6N2E2_5633 0.88 -1.7 6,449,167 + Pf6N2E2_5633 0.88 -3.4 6,449,168 - Pf6N2E2_5633 0.88 -1.3 6,449,168 - Pf6N2E2_5633 0.88 -2.4 6,449,168 - Pf6N2E2_5633 0.88 +1.1 6,449,168 - Pf6N2E2_5633 0.88 -2.1 6,449,168 - Pf6N2E2_5633 0.88 +1.0 6,449,338 + -0.9 6,449,355 - -1.5 6,449,355 - -0.2 6,449,378 + +0.4 6,449,482 + +0.2 6,449,483 - -0.6 6,449,483 - -1.7 6,449,574 - -0.4 6,449,832 + Pf6N2E2_5634 0.37 +0.8 6,449,832 + Pf6N2E2_5634 0.37 +0.3 6,449,833 - Pf6N2E2_5634 0.37 -0.1 6,449,833 - Pf6N2E2_5634 0.37 +0.3 6,449,833 - Pf6N2E2_5634 0.37 -0.5 6,449,833 - Pf6N2E2_5634 0.37 -0.6 6,449,871 - Pf6N2E2_5634 0.46 +0.3 6,449,871 - Pf6N2E2_5634 0.46 -1.0 6,449,884 + Pf6N2E2_5634 0.50 -2.4 6,449,884 + Pf6N2E2_5634 0.50 -1.2 6,449,885 - Pf6N2E2_5634 0.50 +0.0 6,449,885 - Pf6N2E2_5634 0.50 -0.6 6,449,885 - Pf6N2E2_5634 0.50 -0.5 6,449,885 - Pf6N2E2_5634 0.50 -1.1 6,450,091 - +0.1 6,450,091 - +0.5 6,450,091 - -0.9 6,450,133 - +0.1 6,450,138 + +0.3 6,450,138 + -0.4 6,450,138 + +0.3 6,450,138 + -1.2 6,450,138 + +0.2 6,450,138 + +0.4 6,450,138 + +0.5 6,450,139 - -1.4 6,450,270 + Pf6N2E2_5635 0.15 -0.8 6,450,271 - Pf6N2E2_5635 0.15 +0.3
Or see this region's nucleotide sequence