Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_562 and Pf6N2E2_563 are separated by 379 nucleotides Pf6N2E2_563 and Pf6N2E2_564 are separated by 312 nucleotides
Pf6N2E2_562 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 635,624 to 636,577
_562
Pf6N2E2_563 - Serine transporter, at 636,957 to 638,237
_563
Pf6N2E2_564 - Aconitate hydratase 2 (EC 4.2.1.3) @ 2-methylisocitrate dehydratase (EC 4.2.1.99), at 638,550 to 641,159
_564
Position (kb) 636
637
638
639 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 636.036 kb on + strand, within Pf6N2E2_562 at 636.069 kb on + strand, within Pf6N2E2_562 at 636.070 kb on - strand, within Pf6N2E2_562 at 636.076 kb on + strand, within Pf6N2E2_562 at 636.222 kb on - strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.336 kb on - strand, within Pf6N2E2_562 at 636.336 kb on - strand, within Pf6N2E2_562 at 636.336 kb on - strand, within Pf6N2E2_562 at 636.534 kb on + strand at 636.535 kb on - strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.591 kb on - strand at 636.591 kb on - strand at 636.612 kb on + strand at 636.613 kb on - strand at 636.613 kb on - strand at 636.627 kb on + strand at 636.628 kb on - strand at 636.628 kb on - strand at 636.628 kb on - strand at 636.628 kb on - strand at 636.658 kb on + strand at 636.658 kb on + strand at 636.658 kb on + strand at 636.658 kb on + strand at 636.658 kb on + strand at 636.659 kb on - strand at 636.661 kb on + strand at 636.661 kb on + strand at 636.690 kb on + strand at 636.694 kb on + strand at 636.694 kb on + strand at 636.694 kb on + strand at 636.695 kb on - strand at 636.695 kb on - strand at 636.720 kb on + strand at 636.721 kb on - strand at 636.787 kb on + strand at 636.809 kb on + strand at 636.809 kb on + strand at 636.815 kb on + strand at 637.082 kb on + strand at 637.083 kb on - strand at 637.083 kb on - strand at 637.083 kb on - strand at 637.167 kb on + strand, within Pf6N2E2_563 at 637.167 kb on + strand, within Pf6N2E2_563 at 637.167 kb on + strand, within Pf6N2E2_563 at 637.168 kb on - strand, within Pf6N2E2_563 at 637.168 kb on - strand, within Pf6N2E2_563 at 637.168 kb on - strand, within Pf6N2E2_563 at 637.168 kb on - strand, within Pf6N2E2_563 at 637.281 kb on + strand, within Pf6N2E2_563 at 637.282 kb on - strand, within Pf6N2E2_563 at 637.282 kb on - strand, within Pf6N2E2_563 at 637.314 kb on + strand, within Pf6N2E2_563 at 637.314 kb on + strand, within Pf6N2E2_563 at 637.315 kb on - strand, within Pf6N2E2_563 at 637.315 kb on - strand, within Pf6N2E2_563 at 637.315 kb on - strand, within Pf6N2E2_563 at 637.368 kb on - strand, within Pf6N2E2_563 at 637.586 kb on + strand, within Pf6N2E2_563 at 637.586 kb on + strand, within Pf6N2E2_563 at 637.587 kb on - strand, within Pf6N2E2_563 at 637.587 kb on - strand, within Pf6N2E2_563 at 637.830 kb on + strand, within Pf6N2E2_563 at 637.921 kb on - strand, within Pf6N2E2_563 at 637.921 kb on - strand, within Pf6N2E2_563 at 637.921 kb on - strand, within Pf6N2E2_563 at 638.121 kb on + strand at 638.122 kb on - strand at 638.122 kb on - strand at 638.221 kb on - strand at 638.345 kb on + strand at 638.346 kb on - strand at 638.445 kb on + strand at 638.494 kb on + strand at 638.494 kb on + strand at 638.495 kb on - strand at 638.495 kb on - strand at 638.545 kb on - strand at 638.545 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 636,036 + Pf6N2E2_562 0.43 -0.3 636,069 + Pf6N2E2_562 0.47 +0.1 636,070 - Pf6N2E2_562 0.47 +0.2 636,076 + Pf6N2E2_562 0.47 -0.2 636,222 - Pf6N2E2_562 0.63 -0.2 636,335 + Pf6N2E2_562 0.75 +0.4 636,335 + Pf6N2E2_562 0.75 +0.9 636,335 + Pf6N2E2_562 0.75 -0.7 636,335 + Pf6N2E2_562 0.75 +0.5 636,336 - Pf6N2E2_562 0.75 -0.9 636,336 - Pf6N2E2_562 0.75 +0.2 636,336 - Pf6N2E2_562 0.75 +0.5 636,534 + +0.5 636,535 - -0.7 636,590 + -0.4 636,590 + -0.2 636,590 + +1.1 636,590 + -0.5 636,590 + -0.4 636,591 - -0.1 636,591 - +0.2 636,612 + +1.4 636,613 - -0.9 636,613 - +2.4 636,627 + +0.1 636,628 - +0.5 636,628 - -0.1 636,628 - -0.3 636,628 - +0.4 636,658 + +0.9 636,658 + -0.5 636,658 + -0.5 636,658 + -0.5 636,658 + +0.9 636,659 - -2.7 636,661 + -0.2 636,661 + -0.4 636,690 + +0.2 636,694 + -0.4 636,694 + +1.1 636,694 + +0.9 636,695 - -1.5 636,695 - -0.5 636,720 + +0.4 636,721 - -1.0 636,787 + -0.3 636,809 + -1.0 636,809 + -2.5 636,815 + -1.1 637,082 + -1.3 637,083 - +0.2 637,083 - +0.7 637,083 - -1.0 637,167 + Pf6N2E2_563 0.16 +0.3 637,167 + Pf6N2E2_563 0.16 -0.1 637,167 + Pf6N2E2_563 0.16 +0.1 637,168 - Pf6N2E2_563 0.16 +0.0 637,168 - Pf6N2E2_563 0.16 -0.4 637,168 - Pf6N2E2_563 0.16 -0.6 637,168 - Pf6N2E2_563 0.16 -0.3 637,281 + Pf6N2E2_563 0.25 -3.3 637,282 - Pf6N2E2_563 0.25 -1.5 637,282 - Pf6N2E2_563 0.25 -1.8 637,314 + Pf6N2E2_563 0.28 -0.8 637,314 + Pf6N2E2_563 0.28 -1.4 637,315 - Pf6N2E2_563 0.28 +0.3 637,315 - Pf6N2E2_563 0.28 +0.1 637,315 - Pf6N2E2_563 0.28 -0.6 637,368 - Pf6N2E2_563 0.32 -1.3 637,586 + Pf6N2E2_563 0.49 +0.4 637,586 + Pf6N2E2_563 0.49 +0.7 637,587 - Pf6N2E2_563 0.49 -1.0 637,587 - Pf6N2E2_563 0.49 -0.8 637,830 + Pf6N2E2_563 0.68 -0.1 637,921 - Pf6N2E2_563 0.75 -0.0 637,921 - Pf6N2E2_563 0.75 -0.2 637,921 - Pf6N2E2_563 0.75 -0.6 638,121 + -0.6 638,122 - -1.0 638,122 - +0.3 638,221 - -1.3 638,345 + -0.7 638,346 - +0.4 638,445 + +0.5 638,494 + -0.5 638,494 + +0.1 638,495 - -0.3 638,495 - +0.2 638,545 - -0.2 638,545 - +2.2
Or see this region's nucleotide sequence