Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5556 and Pf6N2E2_5557 are separated by 39 nucleotides Pf6N2E2_5557 and Pf6N2E2_5558 are separated by 176 nucleotides
Pf6N2E2_5556 - Aminomethyltransferase (EC 2.1.2.10) (from data) , at 6,371,537 to 6,372,661
_5556
Pf6N2E2_5557 - L-serine ammonia-lyase (EC 4.3.1.17) (from data) , at 6,372,701 to 6,374,077
_5557
Pf6N2E2_5558 - Glycine dehydrogenase (aminomethyl-transferring) (EC 1.4.4.2) (from data) , at 6,374,254 to 6,377,115
_5558
Position (kb)
6372
6373
6374
6375 Strain fitness (log2 ratio)
-1
0
1 at 6371.764 kb on + strand, within Pf6N2E2_5556 at 6371.764 kb on + strand, within Pf6N2E2_5556 at 6371.764 kb on + strand, within Pf6N2E2_5556 at 6371.764 kb on + strand, within Pf6N2E2_5556 at 6371.765 kb on - strand, within Pf6N2E2_5556 at 6371.765 kb on - strand, within Pf6N2E2_5556 at 6371.876 kb on + strand, within Pf6N2E2_5556 at 6371.943 kb on + strand, within Pf6N2E2_5556 at 6372.105 kb on + strand, within Pf6N2E2_5556 at 6372.105 kb on + strand, within Pf6N2E2_5556 at 6372.105 kb on + strand, within Pf6N2E2_5556 at 6372.105 kb on + strand, within Pf6N2E2_5556 at 6372.106 kb on - strand, within Pf6N2E2_5556 at 6372.106 kb on - strand, within Pf6N2E2_5556 at 6372.106 kb on - strand, within Pf6N2E2_5556 at 6372.106 kb on - strand, within Pf6N2E2_5556 at 6372.580 kb on - strand at 6372.598 kb on + strand at 6372.599 kb on - strand at 6372.661 kb on + strand at 6372.661 kb on + strand at 6372.661 kb on + strand at 6372.661 kb on + strand at 6372.661 kb on + strand at 6372.662 kb on - strand at 6372.662 kb on - strand at 6372.662 kb on - strand at 6372.662 kb on - strand at 6372.662 kb on - strand at 6372.662 kb on - strand at 6372.662 kb on - strand at 6372.750 kb on + strand at 6372.750 kb on + strand at 6372.750 kb on + strand at 6372.751 kb on - strand at 6372.751 kb on - strand at 6373.089 kb on + strand, within Pf6N2E2_5557 at 6373.089 kb on + strand, within Pf6N2E2_5557 at 6373.089 kb on + strand, within Pf6N2E2_5557 at 6373.090 kb on - strand, within Pf6N2E2_5557 at 6373.090 kb on - strand, within Pf6N2E2_5557 at 6373.090 kb on - strand, within Pf6N2E2_5557 at 6373.090 kb on - strand, within Pf6N2E2_5557 at 6373.090 kb on - strand, within Pf6N2E2_5557 at 6373.167 kb on + strand, within Pf6N2E2_5557 at 6373.167 kb on + strand, within Pf6N2E2_5557 at 6373.167 kb on + strand, within Pf6N2E2_5557 at 6373.167 kb on + strand, within Pf6N2E2_5557 at 6373.167 kb on + strand, within Pf6N2E2_5557 at 6373.167 kb on + strand, within Pf6N2E2_5557 at 6373.167 kb on + strand, within Pf6N2E2_5557 at 6373.167 kb on + strand, within Pf6N2E2_5557 at 6373.168 kb on - strand, within Pf6N2E2_5557 at 6373.168 kb on - strand, within Pf6N2E2_5557 at 6373.168 kb on - strand, within Pf6N2E2_5557 at 6373.168 kb on - strand, within Pf6N2E2_5557 at 6373.168 kb on - strand, within Pf6N2E2_5557 at 6373.168 kb on - strand, within Pf6N2E2_5557 at 6373.168 kb on - strand, within Pf6N2E2_5557 at 6373.170 kb on + strand, within Pf6N2E2_5557 at 6373.171 kb on - strand, within Pf6N2E2_5557 at 6373.171 kb on - strand, within Pf6N2E2_5557 at 6373.171 kb on - strand, within Pf6N2E2_5557 at 6373.171 kb on - strand, within Pf6N2E2_5557 at 6373.171 kb on - strand, within Pf6N2E2_5557 at 6373.171 kb on - strand, within Pf6N2E2_5557 at 6373.171 kb on - strand, within Pf6N2E2_5557 at 6373.210 kb on + strand, within Pf6N2E2_5557 at 6373.266 kb on + strand, within Pf6N2E2_5557 at 6373.267 kb on - strand, within Pf6N2E2_5557 at 6373.267 kb on - strand, within Pf6N2E2_5557 at 6373.267 kb on - strand, within Pf6N2E2_5557 at 6373.518 kb on + strand, within Pf6N2E2_5557 at 6373.518 kb on + strand, within Pf6N2E2_5557 at 6373.518 kb on + strand, within Pf6N2E2_5557 at 6373.518 kb on + strand, within Pf6N2E2_5557 at 6373.518 kb on + strand, within Pf6N2E2_5557 at 6373.518 kb on + strand, within Pf6N2E2_5557 at 6373.518 kb on + strand, within Pf6N2E2_5557 at 6373.519 kb on - strand, within Pf6N2E2_5557 at 6373.644 kb on + strand, within Pf6N2E2_5557 at 6373.644 kb on + strand, within Pf6N2E2_5557 at 6373.647 kb on + strand, within Pf6N2E2_5557 at 6373.976 kb on + strand at 6373.977 kb on - strand at 6374.021 kb on - strand at 6374.100 kb on - strand at 6374.249 kb on - strand at 6374.261 kb on + strand at 6374.261 kb on + strand at 6374.262 kb on - strand at 6374.262 kb on - strand at 6374.339 kb on + strand at 6374.339 kb on + strand at 6374.339 kb on + strand at 6374.340 kb on - strand at 6374.340 kb on - strand at 6374.390 kb on + strand at 6374.391 kb on - strand at 6374.762 kb on + strand, within Pf6N2E2_5558 at 6374.763 kb on - strand, within Pf6N2E2_5558 at 6374.763 kb on - strand, within Pf6N2E2_5558 at 6374.828 kb on + strand, within Pf6N2E2_5558 at 6374.828 kb on + strand, within Pf6N2E2_5558 at 6374.829 kb on - strand, within Pf6N2E2_5558 at 6374.829 kb on - strand, within Pf6N2E2_5558 at 6374.829 kb on - strand, within Pf6N2E2_5558 at 6374.829 kb on - strand, within Pf6N2E2_5558
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 6,371,764 + Pf6N2E2_5556 0.20 -0.0 6,371,764 + Pf6N2E2_5556 0.20 -0.5 6,371,764 + Pf6N2E2_5556 0.20 +0.5 6,371,764 + Pf6N2E2_5556 0.20 +1.6 6,371,765 - Pf6N2E2_5556 0.20 +0.1 6,371,765 - Pf6N2E2_5556 0.20 +0.6 6,371,876 + Pf6N2E2_5556 0.30 -0.3 6,371,943 + Pf6N2E2_5556 0.36 +0.0 6,372,105 + Pf6N2E2_5556 0.50 +0.9 6,372,105 + Pf6N2E2_5556 0.50 +0.0 6,372,105 + Pf6N2E2_5556 0.50 +0.4 6,372,105 + Pf6N2E2_5556 0.50 +1.2 6,372,106 - Pf6N2E2_5556 0.51 +0.6 6,372,106 - Pf6N2E2_5556 0.51 -0.1 6,372,106 - Pf6N2E2_5556 0.51 +0.9 6,372,106 - Pf6N2E2_5556 0.51 -0.5 6,372,580 - +0.3 6,372,598 + -0.4 6,372,599 - -0.2 6,372,661 + +0.5 6,372,661 + +0.1 6,372,661 + +1.2 6,372,661 + +0.3 6,372,661 + +1.1 6,372,662 - +0.6 6,372,662 - +0.8 6,372,662 - +0.1 6,372,662 - -1.3 6,372,662 - +1.1 6,372,662 - -0.9 6,372,662 - -0.3 6,372,750 + +0.7 6,372,750 + +0.4 6,372,750 + +0.2 6,372,751 - -0.1 6,372,751 - +0.3 6,373,089 + Pf6N2E2_5557 0.28 +0.3 6,373,089 + Pf6N2E2_5557 0.28 -0.4 6,373,089 + Pf6N2E2_5557 0.28 +0.2 6,373,090 - Pf6N2E2_5557 0.28 +0.8 6,373,090 - Pf6N2E2_5557 0.28 +0.7 6,373,090 - Pf6N2E2_5557 0.28 -0.1 6,373,090 - Pf6N2E2_5557 0.28 -0.1 6,373,090 - Pf6N2E2_5557 0.28 -0.3 6,373,167 + Pf6N2E2_5557 0.34 -0.9 6,373,167 + Pf6N2E2_5557 0.34 +1.0 6,373,167 + Pf6N2E2_5557 0.34 +1.2 6,373,167 + Pf6N2E2_5557 0.34 -0.9 6,373,167 + Pf6N2E2_5557 0.34 +0.8 6,373,167 + Pf6N2E2_5557 0.34 +1.2 6,373,167 + Pf6N2E2_5557 0.34 -0.3 6,373,167 + Pf6N2E2_5557 0.34 +0.2 6,373,168 - Pf6N2E2_5557 0.34 +1.2 6,373,168 - Pf6N2E2_5557 0.34 +0.3 6,373,168 - Pf6N2E2_5557 0.34 -0.0 6,373,168 - Pf6N2E2_5557 0.34 -0.5 6,373,168 - Pf6N2E2_5557 0.34 -0.3 6,373,168 - Pf6N2E2_5557 0.34 -0.1 6,373,168 - Pf6N2E2_5557 0.34 +0.2 6,373,170 + Pf6N2E2_5557 0.34 -0.5 6,373,171 - Pf6N2E2_5557 0.34 +1.2 6,373,171 - Pf6N2E2_5557 0.34 +1.0 6,373,171 - Pf6N2E2_5557 0.34 +0.5 6,373,171 - Pf6N2E2_5557 0.34 -0.0 6,373,171 - Pf6N2E2_5557 0.34 +0.4 6,373,171 - Pf6N2E2_5557 0.34 +0.0 6,373,171 - Pf6N2E2_5557 0.34 -1.3 6,373,210 + Pf6N2E2_5557 0.37 +0.9 6,373,266 + Pf6N2E2_5557 0.41 -0.4 6,373,267 - Pf6N2E2_5557 0.41 +0.1 6,373,267 - Pf6N2E2_5557 0.41 +0.9 6,373,267 - Pf6N2E2_5557 0.41 +1.1 6,373,518 + Pf6N2E2_5557 0.59 +0.3 6,373,518 + Pf6N2E2_5557 0.59 +0.3 6,373,518 + Pf6N2E2_5557 0.59 -0.8 6,373,518 + Pf6N2E2_5557 0.59 -1.7 6,373,518 + Pf6N2E2_5557 0.59 +0.7 6,373,518 + Pf6N2E2_5557 0.59 -0.7 6,373,518 + Pf6N2E2_5557 0.59 +0.4 6,373,519 - Pf6N2E2_5557 0.59 -0.3 6,373,644 + Pf6N2E2_5557 0.68 -0.5 6,373,644 + Pf6N2E2_5557 0.68 +0.3 6,373,647 + Pf6N2E2_5557 0.69 -0.4 6,373,976 + +0.4 6,373,977 - -0.2 6,374,021 - -0.0 6,374,100 - +0.1 6,374,249 - -1.3 6,374,261 + +0.7 6,374,261 + +0.0 6,374,262 - +0.6 6,374,262 - +0.8 6,374,339 + -0.2 6,374,339 + +1.2 6,374,339 + -1.1 6,374,340 - -0.6 6,374,340 - -0.6 6,374,390 + -0.9 6,374,391 - +0.4 6,374,762 + Pf6N2E2_5558 0.18 +0.5 6,374,763 - Pf6N2E2_5558 0.18 -0.7 6,374,763 - Pf6N2E2_5558 0.18 +1.0 6,374,828 + Pf6N2E2_5558 0.20 +0.7 6,374,828 + Pf6N2E2_5558 0.20 +1.6 6,374,829 - Pf6N2E2_5558 0.20 +0.3 6,374,829 - Pf6N2E2_5558 0.20 +0.1 6,374,829 - Pf6N2E2_5558 0.20 -1.3 6,374,829 - Pf6N2E2_5558 0.20 -0.7
Or see this region's nucleotide sequence