Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5529 and Pf6N2E2_5530 are separated by 302 nucleotides Pf6N2E2_5530 and Pf6N2E2_5531 are separated by 3 nucleotides Pf6N2E2_5531 and Pf6N2E2_5532 are separated by 21 nucleotides Pf6N2E2_5532 and Pf6N2E2_5533 are separated by 75 nucleotides
Pf6N2E2_5529 - Non-specific DNA-binding protein Dps / Iron-binding ferritin-like antioxidant protein / Ferroxidase (EC 1.16.3.1), at 6,347,486 to 6,347,956
_5529
Pf6N2E2_5530 - FIG00955846: hypothetical protein, at 6,348,259 to 6,348,603
_5530
Pf6N2E2_5531 - hypothetical protein, at 6,348,607 to 6,348,771
_5531
Pf6N2E2_5532 - Glycerate kinase (EC 2.7.1.31), at 6,348,793 to 6,348,972
_5532
Pf6N2E2_5533 - Aspartyl-tRNA synthetase (EC 6.1.1.12) @ Aspartyl-tRNA(Asn) synthetase (EC 6.1.1.23), at 6,349,048 to 6,350,823
_5533
Position (kb)
6348
6349 Strain fitness (log2 ratio)
-1
0
1 at 6347.830 kb on + strand, within Pf6N2E2_5529 at 6347.854 kb on - strand, within Pf6N2E2_5529 at 6347.954 kb on + strand at 6347.954 kb on + strand at 6347.954 kb on + strand at 6347.955 kb on - strand at 6347.958 kb on - strand at 6347.958 kb on - strand at 6347.971 kb on + strand at 6347.971 kb on + strand at 6347.971 kb on + strand at 6347.971 kb on + strand at 6347.971 kb on + strand at 6347.971 kb on + strand at 6347.971 kb on + strand at 6347.971 kb on + strand at 6347.971 kb on + strand at 6347.972 kb on - strand at 6347.972 kb on - strand at 6347.972 kb on - strand at 6347.972 kb on - strand at 6347.972 kb on - strand at 6347.972 kb on - strand at 6347.972 kb on - strand at 6348.105 kb on + strand at 6348.105 kb on + strand at 6348.105 kb on + strand at 6348.106 kb on - strand at 6348.115 kb on + strand at 6348.115 kb on + strand at 6348.115 kb on + strand at 6348.116 kb on - strand at 6348.147 kb on + strand at 6348.147 kb on + strand at 6348.161 kb on + strand at 6348.162 kb on - strand at 6348.236 kb on + strand at 6348.236 kb on + strand at 6348.258 kb on + strand at 6348.258 kb on + strand at 6348.418 kb on + strand, within Pf6N2E2_5530 at 6348.418 kb on + strand, within Pf6N2E2_5530 at 6348.418 kb on + strand, within Pf6N2E2_5530 at 6348.418 kb on + strand, within Pf6N2E2_5530 at 6348.419 kb on - strand, within Pf6N2E2_5530 at 6348.469 kb on - strand, within Pf6N2E2_5530 at 6348.469 kb on - strand, within Pf6N2E2_5530 at 6348.469 kb on - strand, within Pf6N2E2_5530 at 6348.469 kb on - strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.548 kb on + strand, within Pf6N2E2_5530 at 6348.549 kb on - strand, within Pf6N2E2_5530 at 6348.549 kb on - strand, within Pf6N2E2_5530 at 6348.549 kb on - strand, within Pf6N2E2_5530 at 6348.549 kb on - strand, within Pf6N2E2_5530 at 6348.549 kb on - strand, within Pf6N2E2_5530 at 6348.549 kb on - strand, within Pf6N2E2_5530 at 6348.549 kb on - strand, within Pf6N2E2_5530 at 6348.549 kb on - strand, within Pf6N2E2_5530 at 6348.608 kb on + strand at 6348.609 kb on - strand at 6348.658 kb on + strand, within Pf6N2E2_5531 at 6348.679 kb on + strand, within Pf6N2E2_5531 at 6348.679 kb on + strand, within Pf6N2E2_5531 at 6348.686 kb on + strand, within Pf6N2E2_5531 at 6348.686 kb on + strand, within Pf6N2E2_5531 at 6348.688 kb on + strand, within Pf6N2E2_5531 at 6348.688 kb on + strand, within Pf6N2E2_5531 at 6348.728 kb on + strand, within Pf6N2E2_5531 at 6348.760 kb on + strand at 6348.760 kb on + strand at 6348.760 kb on + strand at 6348.760 kb on + strand at 6348.760 kb on + strand at 6348.760 kb on + strand at 6348.760 kb on + strand at 6348.760 kb on + strand at 6348.760 kb on + strand at 6348.766 kb on + strand at 6348.766 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 6,347,830 + Pf6N2E2_5529 0.73 +0.3 6,347,854 - Pf6N2E2_5529 0.78 +1.7 6,347,954 + +0.2 6,347,954 + +0.2 6,347,954 + +0.4 6,347,955 - +0.8 6,347,958 - +0.1 6,347,958 - -0.1 6,347,971 + -0.4 6,347,971 + +0.2 6,347,971 + +1.4 6,347,971 + -0.4 6,347,971 + -1.1 6,347,971 + +1.1 6,347,971 + +1.4 6,347,971 + +1.3 6,347,971 + -0.3 6,347,972 - +0.4 6,347,972 - -1.6 6,347,972 - -0.4 6,347,972 - -0.7 6,347,972 - +0.3 6,347,972 - +1.2 6,347,972 - -1.5 6,348,105 + +1.1 6,348,105 + -0.4 6,348,105 + +0.5 6,348,106 - +0.8 6,348,115 + +0.7 6,348,115 + -1.5 6,348,115 + +0.8 6,348,116 - +0.3 6,348,147 + +0.9 6,348,147 + -0.2 6,348,161 + -0.6 6,348,162 - +0.2 6,348,236 + -0.6 6,348,236 + -0.7 6,348,258 + +0.4 6,348,258 + +0.6 6,348,418 + Pf6N2E2_5530 0.46 -0.4 6,348,418 + Pf6N2E2_5530 0.46 +0.5 6,348,418 + Pf6N2E2_5530 0.46 +1.1 6,348,418 + Pf6N2E2_5530 0.46 -0.1 6,348,419 - Pf6N2E2_5530 0.46 +0.8 6,348,469 - Pf6N2E2_5530 0.61 +0.9 6,348,469 - Pf6N2E2_5530 0.61 +0.5 6,348,469 - Pf6N2E2_5530 0.61 +1.7 6,348,469 - Pf6N2E2_5530 0.61 +1.3 6,348,548 + Pf6N2E2_5530 0.84 +1.3 6,348,548 + Pf6N2E2_5530 0.84 +1.3 6,348,548 + Pf6N2E2_5530 0.84 +0.3 6,348,548 + Pf6N2E2_5530 0.84 +0.3 6,348,548 + Pf6N2E2_5530 0.84 -0.2 6,348,548 + Pf6N2E2_5530 0.84 +0.5 6,348,548 + Pf6N2E2_5530 0.84 -0.7 6,348,548 + Pf6N2E2_5530 0.84 +0.8 6,348,548 + Pf6N2E2_5530 0.84 +0.7 6,348,548 + Pf6N2E2_5530 0.84 +0.2 6,348,548 + Pf6N2E2_5530 0.84 +0.6 6,348,549 - Pf6N2E2_5530 0.84 -0.6 6,348,549 - Pf6N2E2_5530 0.84 -0.6 6,348,549 - Pf6N2E2_5530 0.84 +0.5 6,348,549 - Pf6N2E2_5530 0.84 +0.5 6,348,549 - Pf6N2E2_5530 0.84 -0.3 6,348,549 - Pf6N2E2_5530 0.84 +0.8 6,348,549 - Pf6N2E2_5530 0.84 +0.8 6,348,549 - Pf6N2E2_5530 0.84 +1.0 6,348,608 + -0.9 6,348,609 - +0.6 6,348,658 + Pf6N2E2_5531 0.31 -0.3 6,348,679 + Pf6N2E2_5531 0.44 +0.4 6,348,679 + Pf6N2E2_5531 0.44 -0.5 6,348,686 + Pf6N2E2_5531 0.48 +0.0 6,348,686 + Pf6N2E2_5531 0.48 -0.3 6,348,688 + Pf6N2E2_5531 0.49 +0.1 6,348,688 + Pf6N2E2_5531 0.49 +0.9 6,348,728 + Pf6N2E2_5531 0.73 +0.8 6,348,760 + +0.9 6,348,760 + -0.0 6,348,760 + -0.7 6,348,760 + -0.0 6,348,760 + -0.3 6,348,760 + -0.9 6,348,760 + -0.1 6,348,760 + -0.5 6,348,760 + +0.6 6,348,766 + +0.0 6,348,766 + +0.2
Or see this region's nucleotide sequence