Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5294 and Pf6N2E2_5295 are separated by 259 nucleotides Pf6N2E2_5295 and Pf6N2E2_5296 are separated by 11 nucleotides
Pf6N2E2_5294 - Leucine dehydrogenase (EC 1.4.1.9), at 6,087,348 to 6,088,367
_5294
Pf6N2E2_5295 - Serine hydroxymethyltransferase (EC 2.1.2.1), at 6,088,627 to 6,089,865
_5295
Pf6N2E2_5296 - Threonine synthase (EC 4.2.3.1), at 6,089,877 to 6,091,130
_5296
Position (kb)
6088
6089
6090 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6087.647 kb on + strand, within Pf6N2E2_5294 at 6087.647 kb on + strand, within Pf6N2E2_5294 at 6087.647 kb on + strand, within Pf6N2E2_5294 at 6087.647 kb on + strand, within Pf6N2E2_5294 at 6087.647 kb on + strand, within Pf6N2E2_5294 at 6087.648 kb on - strand, within Pf6N2E2_5294 at 6087.648 kb on - strand, within Pf6N2E2_5294 at 6087.648 kb on - strand, within Pf6N2E2_5294 at 6087.648 kb on - strand, within Pf6N2E2_5294 at 6087.669 kb on + strand, within Pf6N2E2_5294 at 6087.669 kb on + strand, within Pf6N2E2_5294 at 6087.669 kb on + strand, within Pf6N2E2_5294 at 6087.670 kb on - strand, within Pf6N2E2_5294 at 6087.670 kb on - strand, within Pf6N2E2_5294 at 6087.670 kb on - strand, within Pf6N2E2_5294 at 6087.671 kb on + strand, within Pf6N2E2_5294 at 6087.671 kb on + strand, within Pf6N2E2_5294 at 6087.672 kb on - strand, within Pf6N2E2_5294 at 6087.672 kb on - strand, within Pf6N2E2_5294 at 6087.672 kb on - strand, within Pf6N2E2_5294 at 6087.672 kb on - strand, within Pf6N2E2_5294 at 6088.112 kb on + strand, within Pf6N2E2_5294 at 6088.113 kb on - strand, within Pf6N2E2_5294 at 6088.168 kb on - strand, within Pf6N2E2_5294 at 6088.168 kb on - strand, within Pf6N2E2_5294 at 6088.168 kb on - strand, within Pf6N2E2_5294 at 6088.179 kb on + strand, within Pf6N2E2_5294 at 6088.179 kb on + strand, within Pf6N2E2_5294 at 6088.179 kb on + strand, within Pf6N2E2_5294 at 6088.179 kb on + strand, within Pf6N2E2_5294 at 6088.179 kb on + strand, within Pf6N2E2_5294 at 6088.179 kb on + strand, within Pf6N2E2_5294 at 6088.180 kb on - strand, within Pf6N2E2_5294 at 6088.180 kb on - strand, within Pf6N2E2_5294 at 6088.180 kb on - strand, within Pf6N2E2_5294 at 6088.180 kb on - strand, within Pf6N2E2_5294 at 6088.180 kb on - strand, within Pf6N2E2_5294 at 6088.180 kb on - strand, within Pf6N2E2_5294 at 6088.206 kb on + strand, within Pf6N2E2_5294 at 6088.206 kb on + strand, within Pf6N2E2_5294 at 6088.207 kb on - strand, within Pf6N2E2_5294 at 6088.207 kb on - strand, within Pf6N2E2_5294 at 6088.207 kb on - strand, within Pf6N2E2_5294 at 6088.207 kb on - strand, within Pf6N2E2_5294 at 6088.207 kb on - strand, within Pf6N2E2_5294 at 6088.281 kb on - strand at 6088.359 kb on + strand at 6088.360 kb on - strand at 6088.423 kb on + strand at 6088.423 kb on + strand at 6088.491 kb on + strand at 6088.491 kb on + strand at 6088.596 kb on - strand at 6088.629 kb on - strand at 6088.629 kb on - strand at 6088.640 kb on + strand at 6088.640 kb on + strand at 6088.640 kb on + strand at 6088.641 kb on - strand at 6088.903 kb on + strand, within Pf6N2E2_5295 at 6088.904 kb on - strand, within Pf6N2E2_5295 at 6089.028 kb on - strand, within Pf6N2E2_5295 at 6089.268 kb on - strand, within Pf6N2E2_5295 at 6089.291 kb on + strand, within Pf6N2E2_5295 at 6089.462 kb on + strand, within Pf6N2E2_5295 at 6089.462 kb on + strand, within Pf6N2E2_5295 at 6089.554 kb on + strand, within Pf6N2E2_5295 at 6089.612 kb on + strand, within Pf6N2E2_5295 at 6089.612 kb on + strand, within Pf6N2E2_5295 at 6089.613 kb on - strand, within Pf6N2E2_5295 at 6089.688 kb on - strand, within Pf6N2E2_5295 at 6089.688 kb on - strand, within Pf6N2E2_5295 at 6089.691 kb on - strand, within Pf6N2E2_5295 at 6089.717 kb on + strand, within Pf6N2E2_5295 at 6089.717 kb on + strand, within Pf6N2E2_5295 at 6089.718 kb on - strand, within Pf6N2E2_5295 at 6089.768 kb on + strand at 6089.768 kb on + strand at 6089.768 kb on + strand at 6089.768 kb on + strand at 6089.768 kb on + strand at 6089.769 kb on - strand at 6089.769 kb on - strand at 6089.878 kb on + strand at 6089.878 kb on + strand at 6089.882 kb on + strand at 6089.882 kb on + strand at 6089.882 kb on + strand at 6089.882 kb on + strand at 6089.882 kb on + strand at 6089.882 kb on + strand at 6089.882 kb on + strand at 6089.882 kb on + strand at 6089.883 kb on - strand at 6089.883 kb on - strand at 6089.883 kb on - strand at 6089.883 kb on - strand at 6089.883 kb on - strand at 6090.024 kb on + strand, within Pf6N2E2_5296 at 6090.024 kb on + strand, within Pf6N2E2_5296 at 6090.025 kb on - strand, within Pf6N2E2_5296 at 6090.025 kb on - strand, within Pf6N2E2_5296 at 6090.025 kb on - strand, within Pf6N2E2_5296 at 6090.034 kb on + strand, within Pf6N2E2_5296 at 6090.283 kb on + strand, within Pf6N2E2_5296 at 6090.283 kb on + strand, within Pf6N2E2_5296 at 6090.284 kb on - strand, within Pf6N2E2_5296 at 6090.373 kb on + strand, within Pf6N2E2_5296 at 6090.373 kb on + strand, within Pf6N2E2_5296 at 6090.373 kb on + strand, within Pf6N2E2_5296 at 6090.487 kb on + strand, within Pf6N2E2_5296 at 6090.487 kb on + strand, within Pf6N2E2_5296 at 6090.487 kb on + strand, within Pf6N2E2_5296 at 6090.487 kb on + strand, within Pf6N2E2_5296 at 6090.487 kb on + strand, within Pf6N2E2_5296 at 6090.487 kb on + strand, within Pf6N2E2_5296 at 6090.487 kb on + strand, within Pf6N2E2_5296 at 6090.487 kb on + strand, within Pf6N2E2_5296 at 6090.488 kb on - strand, within Pf6N2E2_5296 at 6090.488 kb on - strand, within Pf6N2E2_5296 at 6090.488 kb on - strand, within Pf6N2E2_5296 at 6090.488 kb on - strand, within Pf6N2E2_5296 at 6090.488 kb on - strand, within Pf6N2E2_5296 at 6090.514 kb on + strand, within Pf6N2E2_5296 at 6090.515 kb on - strand, within Pf6N2E2_5296 at 6090.515 kb on - strand, within Pf6N2E2_5296 at 6090.565 kb on + strand, within Pf6N2E2_5296 at 6090.565 kb on + strand, within Pf6N2E2_5296 at 6090.565 kb on + strand, within Pf6N2E2_5296 at 6090.566 kb on - strand, within Pf6N2E2_5296 at 6090.599 kb on - strand, within Pf6N2E2_5296 at 6090.646 kb on + strand, within Pf6N2E2_5296 at 6090.646 kb on + strand, within Pf6N2E2_5296 at 6090.646 kb on + strand, within Pf6N2E2_5296 at 6090.646 kb on + strand, within Pf6N2E2_5296 at 6090.647 kb on - strand, within Pf6N2E2_5296 at 6090.647 kb on - strand, within Pf6N2E2_5296 at 6090.865 kb on + strand, within Pf6N2E2_5296 at 6090.865 kb on + strand, within Pf6N2E2_5296
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 6,087,647 + Pf6N2E2_5294 0.29 +1.0 6,087,647 + Pf6N2E2_5294 0.29 +0.3 6,087,647 + Pf6N2E2_5294 0.29 -0.4 6,087,647 + Pf6N2E2_5294 0.29 -0.1 6,087,647 + Pf6N2E2_5294 0.29 +0.2 6,087,648 - Pf6N2E2_5294 0.29 +0.4 6,087,648 - Pf6N2E2_5294 0.29 +0.2 6,087,648 - Pf6N2E2_5294 0.29 +0.2 6,087,648 - Pf6N2E2_5294 0.29 +0.2 6,087,669 + Pf6N2E2_5294 0.31 -0.7 6,087,669 + Pf6N2E2_5294 0.31 +0.8 6,087,669 + Pf6N2E2_5294 0.31 -0.1 6,087,670 - Pf6N2E2_5294 0.32 -2.1 6,087,670 - Pf6N2E2_5294 0.32 +0.7 6,087,670 - Pf6N2E2_5294 0.32 +0.6 6,087,671 + Pf6N2E2_5294 0.32 +1.1 6,087,671 + Pf6N2E2_5294 0.32 +0.6 6,087,672 - Pf6N2E2_5294 0.32 -1.9 6,087,672 - Pf6N2E2_5294 0.32 +0.7 6,087,672 - Pf6N2E2_5294 0.32 +0.2 6,087,672 - Pf6N2E2_5294 0.32 -1.9 6,088,112 + Pf6N2E2_5294 0.75 +0.5 6,088,113 - Pf6N2E2_5294 0.75 +0.0 6,088,168 - Pf6N2E2_5294 0.80 +1.0 6,088,168 - Pf6N2E2_5294 0.80 +0.7 6,088,168 - Pf6N2E2_5294 0.80 +0.7 6,088,179 + Pf6N2E2_5294 0.81 +1.1 6,088,179 + Pf6N2E2_5294 0.81 +0.2 6,088,179 + Pf6N2E2_5294 0.81 -0.6 6,088,179 + Pf6N2E2_5294 0.81 -0.5 6,088,179 + Pf6N2E2_5294 0.81 -1.0 6,088,179 + Pf6N2E2_5294 0.81 +0.8 6,088,180 - Pf6N2E2_5294 0.82 -0.2 6,088,180 - Pf6N2E2_5294 0.82 -2.4 6,088,180 - Pf6N2E2_5294 0.82 +0.6 6,088,180 - Pf6N2E2_5294 0.82 +0.0 6,088,180 - Pf6N2E2_5294 0.82 -0.2 6,088,180 - Pf6N2E2_5294 0.82 +0.2 6,088,206 + Pf6N2E2_5294 0.84 -0.1 6,088,206 + Pf6N2E2_5294 0.84 -1.7 6,088,207 - Pf6N2E2_5294 0.84 +0.6 6,088,207 - Pf6N2E2_5294 0.84 -0.9 6,088,207 - Pf6N2E2_5294 0.84 -0.6 6,088,207 - Pf6N2E2_5294 0.84 +0.6 6,088,207 - Pf6N2E2_5294 0.84 -0.9 6,088,281 - -0.4 6,088,359 + -0.2 6,088,360 - +0.7 6,088,423 + +0.8 6,088,423 + +0.2 6,088,491 + +0.3 6,088,491 + -1.8 6,088,596 - -0.2 6,088,629 - +0.5 6,088,629 - +0.4 6,088,640 + +0.3 6,088,640 + +0.1 6,088,640 + +0.7 6,088,641 - +0.3 6,088,903 + Pf6N2E2_5295 0.22 -1.0 6,088,904 - Pf6N2E2_5295 0.22 +0.2 6,089,028 - Pf6N2E2_5295 0.32 -0.0 6,089,268 - Pf6N2E2_5295 0.52 -0.3 6,089,291 + Pf6N2E2_5295 0.54 -0.4 6,089,462 + Pf6N2E2_5295 0.67 -0.5 6,089,462 + Pf6N2E2_5295 0.67 -1.7 6,089,554 + Pf6N2E2_5295 0.75 +0.8 6,089,612 + Pf6N2E2_5295 0.79 -0.5 6,089,612 + Pf6N2E2_5295 0.79 +0.8 6,089,613 - Pf6N2E2_5295 0.80 +0.4 6,089,688 - Pf6N2E2_5295 0.86 +0.9 6,089,688 - Pf6N2E2_5295 0.86 +0.9 6,089,691 - Pf6N2E2_5295 0.86 +0.4 6,089,717 + Pf6N2E2_5295 0.88 -0.1 6,089,717 + Pf6N2E2_5295 0.88 +0.0 6,089,718 - Pf6N2E2_5295 0.88 -3.3 6,089,768 + +1.1 6,089,768 + +0.0 6,089,768 + -1.3 6,089,768 + +0.2 6,089,768 + -0.1 6,089,769 - -1.1 6,089,769 - +1.3 6,089,878 + +0.3 6,089,878 + -0.1 6,089,882 + +0.7 6,089,882 + -0.1 6,089,882 + -0.9 6,089,882 + +0.1 6,089,882 + -1.5 6,089,882 + +0.4 6,089,882 + +0.3 6,089,882 + +0.2 6,089,883 - +1.2 6,089,883 - -0.0 6,089,883 - -0.0 6,089,883 - -0.2 6,089,883 - -0.6 6,090,024 + Pf6N2E2_5296 0.12 +1.1 6,090,024 + Pf6N2E2_5296 0.12 +0.5 6,090,025 - Pf6N2E2_5296 0.12 -0.1 6,090,025 - Pf6N2E2_5296 0.12 +0.9 6,090,025 - Pf6N2E2_5296 0.12 +1.7 6,090,034 + Pf6N2E2_5296 0.13 -1.0 6,090,283 + Pf6N2E2_5296 0.32 -0.9 6,090,283 + Pf6N2E2_5296 0.32 -0.2 6,090,284 - Pf6N2E2_5296 0.32 +0.9 6,090,373 + Pf6N2E2_5296 0.40 +0.6 6,090,373 + Pf6N2E2_5296 0.40 +1.3 6,090,373 + Pf6N2E2_5296 0.40 +0.3 6,090,487 + Pf6N2E2_5296 0.49 +1.3 6,090,487 + Pf6N2E2_5296 0.49 -0.1 6,090,487 + Pf6N2E2_5296 0.49 +1.4 6,090,487 + Pf6N2E2_5296 0.49 -0.9 6,090,487 + Pf6N2E2_5296 0.49 +0.4 6,090,487 + Pf6N2E2_5296 0.49 +0.1 6,090,487 + Pf6N2E2_5296 0.49 +0.1 6,090,487 + Pf6N2E2_5296 0.49 +1.2 6,090,488 - Pf6N2E2_5296 0.49 +0.6 6,090,488 - Pf6N2E2_5296 0.49 +0.6 6,090,488 - Pf6N2E2_5296 0.49 +0.2 6,090,488 - Pf6N2E2_5296 0.49 -0.4 6,090,488 - Pf6N2E2_5296 0.49 -0.9 6,090,514 + Pf6N2E2_5296 0.51 -0.4 6,090,515 - Pf6N2E2_5296 0.51 -0.2 6,090,515 - Pf6N2E2_5296 0.51 -1.3 6,090,565 + Pf6N2E2_5296 0.55 +0.1 6,090,565 + Pf6N2E2_5296 0.55 +0.7 6,090,565 + Pf6N2E2_5296 0.55 +0.5 6,090,566 - Pf6N2E2_5296 0.55 -0.1 6,090,599 - Pf6N2E2_5296 0.58 +0.5 6,090,646 + Pf6N2E2_5296 0.61 +0.5 6,090,646 + Pf6N2E2_5296 0.61 +2.0 6,090,646 + Pf6N2E2_5296 0.61 +0.3 6,090,646 + Pf6N2E2_5296 0.61 -0.4 6,090,647 - Pf6N2E2_5296 0.61 +0.7 6,090,647 - Pf6N2E2_5296 0.61 +2.3 6,090,865 + Pf6N2E2_5296 0.79 -0.7 6,090,865 + Pf6N2E2_5296 0.79 -0.2
Or see this region's nucleotide sequence