Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5266 and Pf6N2E2_5267 are separated by 40 nucleotides Pf6N2E2_5267 and Pf6N2E2_5268 are separated by 310 nucleotides
Pf6N2E2_5266 - Glycosyltransferase, at 6,060,149 to 6,061,348
_5266
Pf6N2E2_5267 - Sulfate transporter, CysZ-type, at 6,061,389 to 6,062,144
_5267
Pf6N2E2_5268 - Thioredoxin reductase (EC 1.8.1.9), at 6,062,455 to 6,063,417
_5268
Position (kb)
6061
6062
6063 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 6060.405 kb on + strand, within Pf6N2E2_5266 at 6060.405 kb on + strand, within Pf6N2E2_5266 at 6060.406 kb on - strand, within Pf6N2E2_5266 at 6060.406 kb on - strand, within Pf6N2E2_5266 at 6060.501 kb on + strand, within Pf6N2E2_5266 at 6060.501 kb on + strand, within Pf6N2E2_5266 at 6060.894 kb on + strand, within Pf6N2E2_5266 at 6060.894 kb on + strand, within Pf6N2E2_5266 at 6060.895 kb on - strand, within Pf6N2E2_5266 at 6060.895 kb on - strand, within Pf6N2E2_5266 at 6060.895 kb on - strand, within Pf6N2E2_5266 at 6060.906 kb on - strand, within Pf6N2E2_5266 at 6060.906 kb on - strand, within Pf6N2E2_5266 at 6060.942 kb on + strand, within Pf6N2E2_5266 at 6060.942 kb on + strand, within Pf6N2E2_5266 at 6060.943 kb on - strand, within Pf6N2E2_5266 at 6060.943 kb on - strand, within Pf6N2E2_5266 at 6060.943 kb on - strand, within Pf6N2E2_5266 at 6060.982 kb on + strand, within Pf6N2E2_5266 at 6061.041 kb on + strand, within Pf6N2E2_5266 at 6061.041 kb on + strand, within Pf6N2E2_5266 at 6061.042 kb on - strand, within Pf6N2E2_5266 at 6061.042 kb on - strand, within Pf6N2E2_5266 at 6061.042 kb on - strand, within Pf6N2E2_5266 at 6061.199 kb on + strand, within Pf6N2E2_5266 at 6061.303 kb on + strand at 6061.304 kb on - strand at 6061.609 kb on + strand, within Pf6N2E2_5267 at 6061.610 kb on - strand, within Pf6N2E2_5267 at 6061.610 kb on - strand, within Pf6N2E2_5267 at 6061.610 kb on - strand, within Pf6N2E2_5267 at 6061.610 kb on - strand, within Pf6N2E2_5267 at 6061.720 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6062.014 kb on + strand, within Pf6N2E2_5267 at 6062.015 kb on - strand, within Pf6N2E2_5267 at 6062.015 kb on - strand, within Pf6N2E2_5267 at 6062.061 kb on + strand, within Pf6N2E2_5267 at 6062.061 kb on + strand, within Pf6N2E2_5267 at 6062.062 kb on - strand, within Pf6N2E2_5267 at 6062.062 kb on - strand, within Pf6N2E2_5267 at 6062.062 kb on - strand, within Pf6N2E2_5267 at 6062.114 kb on - strand at 6062.144 kb on + strand at 6062.144 kb on + strand at 6062.144 kb on + strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.220 kb on + strand at 6062.220 kb on + strand at 6062.220 kb on + strand at 6062.220 kb on + strand at 6062.220 kb on + strand at 6062.220 kb on + strand at 6062.220 kb on + strand at 6062.221 kb on - strand at 6062.221 kb on - strand at 6062.249 kb on - strand at 6062.269 kb on + strand at 6062.280 kb on + strand at 6062.295 kb on + strand at 6062.295 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 6,060,405 + Pf6N2E2_5266 0.21 +0.4 6,060,405 + Pf6N2E2_5266 0.21 -0.3 6,060,406 - Pf6N2E2_5266 0.21 -0.2 6,060,406 - Pf6N2E2_5266 0.21 -0.6 6,060,501 + Pf6N2E2_5266 0.29 +0.6 6,060,501 + Pf6N2E2_5266 0.29 -0.3 6,060,894 + Pf6N2E2_5266 0.62 +0.7 6,060,894 + Pf6N2E2_5266 0.62 +0.3 6,060,895 - Pf6N2E2_5266 0.62 +0.0 6,060,895 - Pf6N2E2_5266 0.62 -0.2 6,060,895 - Pf6N2E2_5266 0.62 +0.5 6,060,906 - Pf6N2E2_5266 0.63 +1.7 6,060,906 - Pf6N2E2_5266 0.63 +0.2 6,060,942 + Pf6N2E2_5266 0.66 +0.4 6,060,942 + Pf6N2E2_5266 0.66 +0.1 6,060,943 - Pf6N2E2_5266 0.66 +0.2 6,060,943 - Pf6N2E2_5266 0.66 +0.6 6,060,943 - Pf6N2E2_5266 0.66 -1.9 6,060,982 + Pf6N2E2_5266 0.69 -0.6 6,061,041 + Pf6N2E2_5266 0.74 +0.5 6,061,041 + Pf6N2E2_5266 0.74 +0.8 6,061,042 - Pf6N2E2_5266 0.74 -0.5 6,061,042 - Pf6N2E2_5266 0.74 +0.5 6,061,042 - Pf6N2E2_5266 0.74 -0.7 6,061,199 + Pf6N2E2_5266 0.88 +0.7 6,061,303 + +0.7 6,061,304 - +0.6 6,061,609 + Pf6N2E2_5267 0.29 -3.6 6,061,610 - Pf6N2E2_5267 0.29 -1.1 6,061,610 - Pf6N2E2_5267 0.29 -0.8 6,061,610 - Pf6N2E2_5267 0.29 -0.2 6,061,610 - Pf6N2E2_5267 0.29 -3.4 6,061,720 + Pf6N2E2_5267 0.44 +0.4 6,061,936 + Pf6N2E2_5267 0.72 -2.0 6,061,936 + Pf6N2E2_5267 0.72 -2.8 6,061,936 + Pf6N2E2_5267 0.72 -2.5 6,061,936 + Pf6N2E2_5267 0.72 -1.4 6,061,936 + Pf6N2E2_5267 0.72 -1.9 6,061,936 + Pf6N2E2_5267 0.72 -0.4 6,061,936 + Pf6N2E2_5267 0.72 -2.3 6,061,936 + Pf6N2E2_5267 0.72 -0.3 6,061,936 + Pf6N2E2_5267 0.72 -0.2 6,061,937 - Pf6N2E2_5267 0.72 -1.5 6,061,937 - Pf6N2E2_5267 0.72 -1.3 6,061,937 - Pf6N2E2_5267 0.72 -1.2 6,061,937 - Pf6N2E2_5267 0.72 -0.6 6,061,937 - Pf6N2E2_5267 0.72 -1.8 6,061,937 - Pf6N2E2_5267 0.72 -2.0 6,061,937 - Pf6N2E2_5267 0.72 -2.8 6,061,937 - Pf6N2E2_5267 0.72 -2.5 6,061,937 - Pf6N2E2_5267 0.72 -2.0 6,062,014 + Pf6N2E2_5267 0.83 -0.2 6,062,015 - Pf6N2E2_5267 0.83 -3.0 6,062,015 - Pf6N2E2_5267 0.83 -1.2 6,062,061 + Pf6N2E2_5267 0.89 -2.3 6,062,061 + Pf6N2E2_5267 0.89 -1.7 6,062,062 - Pf6N2E2_5267 0.89 -1.7 6,062,062 - Pf6N2E2_5267 0.89 -1.7 6,062,062 - Pf6N2E2_5267 0.89 -1.4 6,062,114 - -3.4 6,062,144 + -2.6 6,062,144 + +1.3 6,062,144 + -0.3 6,062,145 - -0.2 6,062,145 - -2.0 6,062,145 - -1.6 6,062,145 - -1.9 6,062,145 - -2.1 6,062,145 - -1.3 6,062,145 - -1.6 6,062,145 - -1.7 6,062,145 - -0.4 6,062,145 - -0.8 6,062,220 + -0.5 6,062,220 + +1.3 6,062,220 + +0.3 6,062,220 + -1.5 6,062,220 + +0.5 6,062,220 + -0.4 6,062,220 + -0.8 6,062,221 - +1.4 6,062,221 - +1.4 6,062,249 - -0.0 6,062,269 + -1.2 6,062,280 + +0.2 6,062,295 + -1.4 6,062,295 + -0.2
Or see this region's nucleotide sequence