Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5080 and Pf6N2E2_5081 are separated by 140 nucleotides Pf6N2E2_5081 and Pf6N2E2_5082 are separated by 316 nucleotides Pf6N2E2_5082 and Pf6N2E2_5083 are separated by 224 nucleotides
Pf6N2E2_5080 - Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family, at 5,857,079 to 5,858,047
_5080
Pf6N2E2_5081 - hypothetical protein, at 5,858,188 to 5,858,631
_5081
Pf6N2E2_5082 - Serine hydroxymethyltransferase (EC 2.1.2.1), at 5,858,948 to 5,860,201
_5082
Pf6N2E2_5083 - Serine hydroxymethyltransferase (EC 2.1.2.1), at 5,860,426 to 5,864,202
_5083
Position (kb)
5858
5859
5860
5861 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5857.983 kb on + strand at 5857.983 kb on + strand at 5857.983 kb on + strand at 5857.984 kb on - strand at 5857.984 kb on - strand at 5857.984 kb on - strand at 5857.984 kb on - strand at 5858.153 kb on - strand at 5858.200 kb on + strand at 5858.201 kb on - strand at 5858.243 kb on + strand, within Pf6N2E2_5081 at 5858.243 kb on + strand, within Pf6N2E2_5081 at 5858.269 kb on + strand, within Pf6N2E2_5081 at 5858.404 kb on + strand, within Pf6N2E2_5081 at 5858.450 kb on + strand, within Pf6N2E2_5081 at 5858.450 kb on + strand, within Pf6N2E2_5081 at 5858.640 kb on + strand at 5858.723 kb on + strand at 5858.752 kb on - strand at 5858.775 kb on + strand at 5858.803 kb on + strand at 5858.809 kb on + strand at 5858.893 kb on + strand at 5858.930 kb on + strand at 5860.266 kb on + strand at 5860.266 kb on + strand at 5860.267 kb on - strand at 5860.267 kb on - strand at 5860.503 kb on - strand at 5860.706 kb on + strand at 5860.707 kb on - strand at 5860.816 kb on - strand, within Pf6N2E2_5083 at 5860.872 kb on + strand, within Pf6N2E2_5083 at 5860.873 kb on - strand, within Pf6N2E2_5083 at 5861.147 kb on + strand, within Pf6N2E2_5083 at 5861.148 kb on - strand, within Pf6N2E2_5083 at 5861.150 kb on + strand, within Pf6N2E2_5083 at 5861.151 kb on - strand, within Pf6N2E2_5083
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 5,857,983 + -0.2 5,857,983 + -0.1 5,857,983 + +0.8 5,857,984 - +2.0 5,857,984 - +0.6 5,857,984 - -2.1 5,857,984 - +0.4 5,858,153 - -0.4 5,858,200 + -0.5 5,858,201 - +0.4 5,858,243 + Pf6N2E2_5081 0.12 +1.8 5,858,243 + Pf6N2E2_5081 0.12 -0.4 5,858,269 + Pf6N2E2_5081 0.18 -0.3 5,858,404 + Pf6N2E2_5081 0.49 -1.7 5,858,450 + Pf6N2E2_5081 0.59 -1.4 5,858,450 + Pf6N2E2_5081 0.59 -1.0 5,858,640 + +0.3 5,858,723 + +0.8 5,858,752 - +0.7 5,858,775 + +0.3 5,858,803 + +1.0 5,858,809 + -0.6 5,858,893 + -1.1 5,858,930 + -0.4 5,860,266 + -0.0 5,860,266 + -0.7 5,860,267 - -0.3 5,860,267 - +0.6 5,860,503 - +0.1 5,860,706 + -0.2 5,860,707 - -0.8 5,860,816 - Pf6N2E2_5083 0.10 +0.5 5,860,872 + Pf6N2E2_5083 0.12 -0.5 5,860,873 - Pf6N2E2_5083 0.12 +0.4 5,861,147 + Pf6N2E2_5083 0.19 -1.8 5,861,148 - Pf6N2E2_5083 0.19 +2.4 5,861,150 + Pf6N2E2_5083 0.19 -1.0 5,861,151 - Pf6N2E2_5083 0.19 -3.1
Or see this region's nucleotide sequence