Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5006 and Pf6N2E2_5007 are separated by 112 nucleotides Pf6N2E2_5007 and Pf6N2E2_5008 are separated by 72 nucleotides Pf6N2E2_5008 and Pf6N2E2_5009 are separated by 276 nucleotides
Pf6N2E2_5006 - Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13), at 5,771,651 to 5,772,595
_5006
Pf6N2E2_5007 - Membrane-bound lytic murein transglycosylase B precursor (EC 3.2.1.-), at 5,772,708 to 5,774,030
_5007
Pf6N2E2_5008 - FIG00957593: hypothetical protein, at 5,774,103 to 5,774,267
_5008
Pf6N2E2_5009 - DNA polymerase III delta subunit (EC 2.7.7.7), at 5,774,544 to 5,775,581
_5009
Position (kb)
5772
5773
5774
5775 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5771.831 kb on + strand, within Pf6N2E2_5006 at 5771.831 kb on + strand, within Pf6N2E2_5006 at 5771.831 kb on + strand, within Pf6N2E2_5006 at 5771.832 kb on - strand, within Pf6N2E2_5006 at 5771.832 kb on - strand, within Pf6N2E2_5006 at 5771.832 kb on - strand, within Pf6N2E2_5006 at 5771.853 kb on + strand, within Pf6N2E2_5006 at 5771.854 kb on - strand, within Pf6N2E2_5006 at 5771.854 kb on - strand, within Pf6N2E2_5006 at 5771.991 kb on + strand, within Pf6N2E2_5006 at 5771.991 kb on + strand, within Pf6N2E2_5006 at 5771.996 kb on + strand, within Pf6N2E2_5006 at 5771.997 kb on - strand, within Pf6N2E2_5006 at 5772.159 kb on + strand, within Pf6N2E2_5006 at 5772.159 kb on + strand, within Pf6N2E2_5006 at 5772.160 kb on - strand, within Pf6N2E2_5006 at 5772.160 kb on - strand, within Pf6N2E2_5006 at 5772.160 kb on - strand, within Pf6N2E2_5006 at 5772.176 kb on + strand, within Pf6N2E2_5006 at 5772.176 kb on + strand, within Pf6N2E2_5006 at 5772.177 kb on - strand, within Pf6N2E2_5006 at 5772.323 kb on + strand, within Pf6N2E2_5006 at 5772.378 kb on + strand, within Pf6N2E2_5006 at 5772.594 kb on - strand at 5772.601 kb on + strand at 5772.602 kb on - strand at 5772.602 kb on - strand at 5772.700 kb on - strand at 5772.922 kb on + strand, within Pf6N2E2_5007 at 5772.923 kb on - strand, within Pf6N2E2_5007 at 5772.958 kb on - strand, within Pf6N2E2_5007 at 5772.976 kb on + strand, within Pf6N2E2_5007 at 5772.977 kb on - strand, within Pf6N2E2_5007 at 5772.994 kb on + strand, within Pf6N2E2_5007 at 5772.994 kb on + strand, within Pf6N2E2_5007 at 5772.994 kb on + strand, within Pf6N2E2_5007 at 5772.994 kb on + strand, within Pf6N2E2_5007 at 5772.994 kb on + strand, within Pf6N2E2_5007 at 5772.994 kb on + strand, within Pf6N2E2_5007 at 5772.995 kb on - strand, within Pf6N2E2_5007 at 5773.111 kb on + strand, within Pf6N2E2_5007 at 5773.112 kb on - strand, within Pf6N2E2_5007 at 5773.177 kb on + strand, within Pf6N2E2_5007 at 5773.250 kb on - strand, within Pf6N2E2_5007 at 5773.250 kb on - strand, within Pf6N2E2_5007 at 5773.336 kb on + strand, within Pf6N2E2_5007 at 5773.336 kb on + strand, within Pf6N2E2_5007 at 5773.336 kb on + strand, within Pf6N2E2_5007 at 5773.336 kb on + strand, within Pf6N2E2_5007 at 5773.336 kb on + strand, within Pf6N2E2_5007 at 5773.337 kb on - strand, within Pf6N2E2_5007 at 5773.337 kb on - strand, within Pf6N2E2_5007 at 5773.337 kb on - strand, within Pf6N2E2_5007 at 5773.514 kb on + strand, within Pf6N2E2_5007 at 5773.514 kb on + strand, within Pf6N2E2_5007 at 5773.596 kb on - strand, within Pf6N2E2_5007 at 5773.596 kb on - strand, within Pf6N2E2_5007 at 5773.596 kb on - strand, within Pf6N2E2_5007 at 5773.596 kb on - strand, within Pf6N2E2_5007 at 5773.675 kb on + strand, within Pf6N2E2_5007 at 5773.676 kb on - strand, within Pf6N2E2_5007 at 5773.676 kb on - strand, within Pf6N2E2_5007 at 5773.985 kb on + strand at 5773.985 kb on + strand at 5774.067 kb on - strand at 5774.085 kb on - strand at 5774.381 kb on - strand at 5774.391 kb on + strand at 5774.391 kb on + strand at 5774.391 kb on + strand at 5774.391 kb on + strand at 5774.392 kb on - strand at 5774.392 kb on - strand at 5774.411 kb on - strand at 5774.443 kb on + strand at 5774.474 kb on - strand at 5774.519 kb on + strand at 5774.519 kb on + strand at 5774.519 kb on + strand at 5774.519 kb on + strand at 5774.519 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 5,771,831 + Pf6N2E2_5006 0.19 -2.5 5,771,831 + Pf6N2E2_5006 0.19 -2.6 5,771,831 + Pf6N2E2_5006 0.19 -3.3 5,771,832 - Pf6N2E2_5006 0.19 -4.0 5,771,832 - Pf6N2E2_5006 0.19 -3.7 5,771,832 - Pf6N2E2_5006 0.19 -2.0 5,771,853 + Pf6N2E2_5006 0.21 -3.9 5,771,854 - Pf6N2E2_5006 0.21 -0.2 5,771,854 - Pf6N2E2_5006 0.21 -2.6 5,771,991 + Pf6N2E2_5006 0.36 -3.0 5,771,991 + Pf6N2E2_5006 0.36 -1.6 5,771,996 + Pf6N2E2_5006 0.37 -2.4 5,771,997 - Pf6N2E2_5006 0.37 -4.1 5,772,159 + Pf6N2E2_5006 0.54 -3.1 5,772,159 + Pf6N2E2_5006 0.54 -2.3 5,772,160 - Pf6N2E2_5006 0.54 -2.7 5,772,160 - Pf6N2E2_5006 0.54 -3.6 5,772,160 - Pf6N2E2_5006 0.54 -3.6 5,772,176 + Pf6N2E2_5006 0.56 -4.1 5,772,176 + Pf6N2E2_5006 0.56 -2.7 5,772,177 - Pf6N2E2_5006 0.56 -3.6 5,772,323 + Pf6N2E2_5006 0.71 -2.9 5,772,378 + Pf6N2E2_5006 0.77 -0.9 5,772,594 - +0.3 5,772,601 + +0.3 5,772,602 - +1.0 5,772,602 - +0.9 5,772,700 - +0.4 5,772,922 + Pf6N2E2_5007 0.16 -1.2 5,772,923 - Pf6N2E2_5007 0.16 -1.8 5,772,958 - Pf6N2E2_5007 0.19 -1.6 5,772,976 + Pf6N2E2_5007 0.20 -0.4 5,772,977 - Pf6N2E2_5007 0.20 -0.7 5,772,994 + Pf6N2E2_5007 0.22 -0.9 5,772,994 + Pf6N2E2_5007 0.22 -1.1 5,772,994 + Pf6N2E2_5007 0.22 +0.6 5,772,994 + Pf6N2E2_5007 0.22 -1.6 5,772,994 + Pf6N2E2_5007 0.22 -1.1 5,772,994 + Pf6N2E2_5007 0.22 -1.6 5,772,995 - Pf6N2E2_5007 0.22 -0.9 5,773,111 + Pf6N2E2_5007 0.30 -0.9 5,773,112 - Pf6N2E2_5007 0.31 -2.0 5,773,177 + Pf6N2E2_5007 0.35 -1.5 5,773,250 - Pf6N2E2_5007 0.41 -1.8 5,773,250 - Pf6N2E2_5007 0.41 -1.0 5,773,336 + Pf6N2E2_5007 0.47 -3.5 5,773,336 + Pf6N2E2_5007 0.47 -1.5 5,773,336 + Pf6N2E2_5007 0.47 -0.4 5,773,336 + Pf6N2E2_5007 0.47 +0.7 5,773,336 + Pf6N2E2_5007 0.47 -1.0 5,773,337 - Pf6N2E2_5007 0.48 -2.2 5,773,337 - Pf6N2E2_5007 0.48 -0.5 5,773,337 - Pf6N2E2_5007 0.48 -1.0 5,773,514 + Pf6N2E2_5007 0.61 -1.2 5,773,514 + Pf6N2E2_5007 0.61 -0.4 5,773,596 - Pf6N2E2_5007 0.67 -0.9 5,773,596 - Pf6N2E2_5007 0.67 -2.5 5,773,596 - Pf6N2E2_5007 0.67 -1.3 5,773,596 - Pf6N2E2_5007 0.67 -1.8 5,773,675 + Pf6N2E2_5007 0.73 +0.3 5,773,676 - Pf6N2E2_5007 0.73 -1.2 5,773,676 - Pf6N2E2_5007 0.73 -1.4 5,773,985 + +0.2 5,773,985 + -0.6 5,774,067 - -0.2 5,774,085 - -1.5 5,774,381 - +0.4 5,774,391 + +1.0 5,774,391 + -2.0 5,774,391 + +0.8 5,774,391 + +0.2 5,774,392 - -0.9 5,774,392 - +0.9 5,774,411 - +0.0 5,774,443 + +0.6 5,774,474 - -1.1 5,774,519 + -0.8 5,774,519 + -0.6 5,774,519 + -0.1 5,774,519 + +0.4 5,774,519 + +0.3
Or see this region's nucleotide sequence