Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_46 and Pf6N2E2_47 overlap by 4 nucleotides Pf6N2E2_47 and Pf6N2E2_48 are separated by 89 nucleotides Pf6N2E2_48 and Pf6N2E2_6095 are separated by 251 nucleotides Pf6N2E2_6095 and Pf6N2E2_49 are separated by 235 nucleotides
Pf6N2E2_46 - membrane protein, putative, at 86,264 to 87,325
_46
Pf6N2E2_47 - FIG00961567: hypothetical protein, at 87,322 to 87,504
_47
Pf6N2E2_48 - FIG00955661: hypothetical protein, at 87,594 to 87,788
_48
Pf6N2E2_6095 - tRNA-Asn-GTT, at 88,040 to 88,112
_6095
Pf6N2E2_49 - FIG00967200: hypothetical protein, at 88,348 to 88,575
_49
Position (kb) 87
88 Strain fitness (log2 ratio) -2
-1
0
1 at 86.506 kb on + strand, within Pf6N2E2_46 at 86.506 kb on + strand, within Pf6N2E2_46 at 86.537 kb on + strand, within Pf6N2E2_46 at 86.537 kb on + strand, within Pf6N2E2_46 at 86.538 kb on - strand, within Pf6N2E2_46 at 86.538 kb on - strand, within Pf6N2E2_46 at 86.771 kb on + strand, within Pf6N2E2_46 at 86.771 kb on + strand, within Pf6N2E2_46 at 86.772 kb on - strand, within Pf6N2E2_46 at 86.772 kb on - strand, within Pf6N2E2_46 at 86.816 kb on - strand, within Pf6N2E2_46 at 87.059 kb on + strand, within Pf6N2E2_46 at 87.060 kb on - strand, within Pf6N2E2_46 at 87.185 kb on + strand, within Pf6N2E2_46 at 87.185 kb on + strand, within Pf6N2E2_46 at 87.185 kb on + strand, within Pf6N2E2_46 at 87.185 kb on + strand, within Pf6N2E2_46 at 87.185 kb on + strand, within Pf6N2E2_46 at 87.186 kb on - strand, within Pf6N2E2_46 at 87.186 kb on - strand, within Pf6N2E2_46 at 87.186 kb on - strand, within Pf6N2E2_46 at 87.323 kb on + strand at 87.338 kb on + strand at 87.339 kb on - strand at 87.531 kb on + strand at 87.531 kb on + strand at 87.536 kb on + strand at 87.536 kb on + strand at 87.536 kb on + strand at 87.575 kb on + strand at 87.575 kb on + strand at 87.575 kb on + strand at 87.576 kb on - strand at 87.578 kb on + strand at 87.579 kb on - strand at 87.733 kb on + strand, within Pf6N2E2_48 at 87.734 kb on - strand, within Pf6N2E2_48 at 87.734 kb on - strand, within Pf6N2E2_48 at 87.788 kb on + strand at 87.788 kb on + strand at 87.806 kb on + strand at 87.806 kb on + strand at 87.807 kb on - strand at 87.906 kb on + strand at 87.928 kb on + strand at 87.929 kb on - strand at 87.975 kb on + strand at 88.034 kb on - strand at 88.077 kb on - strand, within Pf6N2E2_6095 at 88.077 kb on - strand, within Pf6N2E2_6095 at 88.093 kb on - strand, within Pf6N2E2_6095 at 88.093 kb on - strand, within Pf6N2E2_6095 at 88.126 kb on + strand at 88.233 kb on + strand at 88.234 kb on - strand at 88.285 kb on + strand at 88.286 kb on - strand at 88.286 kb on - strand at 88.286 kb on - strand at 88.286 kb on - strand at 88.291 kb on - strand at 88.291 kb on - strand at 88.311 kb on + strand at 88.312 kb on - strand at 88.405 kb on + strand, within Pf6N2E2_49 at 88.406 kb on - strand, within Pf6N2E2_49 at 88.406 kb on - strand, within Pf6N2E2_49 at 88.491 kb on + strand, within Pf6N2E2_49
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 86,506 + Pf6N2E2_46 0.23 +0.3 86,506 + Pf6N2E2_46 0.23 -0.1 86,537 + Pf6N2E2_46 0.26 -1.4 86,537 + Pf6N2E2_46 0.26 -1.3 86,538 - Pf6N2E2_46 0.26 +0.3 86,538 - Pf6N2E2_46 0.26 +0.2 86,771 + Pf6N2E2_46 0.48 +1.0 86,771 + Pf6N2E2_46 0.48 +0.1 86,772 - Pf6N2E2_46 0.48 +0.8 86,772 - Pf6N2E2_46 0.48 +0.5 86,816 - Pf6N2E2_46 0.52 -1.7 87,059 + Pf6N2E2_46 0.75 -0.0 87,060 - Pf6N2E2_46 0.75 +0.6 87,185 + Pf6N2E2_46 0.87 -0.1 87,185 + Pf6N2E2_46 0.87 -1.2 87,185 + Pf6N2E2_46 0.87 +0.8 87,185 + Pf6N2E2_46 0.87 +0.9 87,185 + Pf6N2E2_46 0.87 +1.2 87,186 - Pf6N2E2_46 0.87 +0.8 87,186 - Pf6N2E2_46 0.87 -1.2 87,186 - Pf6N2E2_46 0.87 +1.2 87,323 + +1.1 87,338 + +0.9 87,339 - +0.7 87,531 + -0.5 87,531 + +0.9 87,536 + -1.1 87,536 + +1.4 87,536 + -0.6 87,575 + -0.9 87,575 + +0.4 87,575 + +0.2 87,576 - -0.3 87,578 + +0.4 87,579 - +0.7 87,733 + Pf6N2E2_48 0.71 +0.1 87,734 - Pf6N2E2_48 0.72 -1.4 87,734 - Pf6N2E2_48 0.72 +0.1 87,788 + +0.2 87,788 + +0.1 87,806 + -1.0 87,806 + -0.9 87,807 - -1.0 87,906 + -0.4 87,928 + -0.2 87,929 - +1.2 87,975 + +0.2 88,034 - +0.5 88,077 - Pf6N2E2_6095 0.51 +0.3 88,077 - Pf6N2E2_6095 0.51 +0.5 88,093 - Pf6N2E2_6095 0.73 +0.8 88,093 - Pf6N2E2_6095 0.73 -0.6 88,126 + -0.5 88,233 + +0.1 88,234 - -2.0 88,285 + +0.9 88,286 - -0.6 88,286 - +0.6 88,286 - +1.2 88,286 - +0.2 88,291 - -0.8 88,291 - -0.6 88,311 + -0.1 88,312 - -0.4 88,405 + Pf6N2E2_49 0.25 +0.4 88,406 - Pf6N2E2_49 0.25 +0.4 88,406 - Pf6N2E2_49 0.25 +1.3 88,491 + Pf6N2E2_49 0.63 +0.1
Or see this region's nucleotide sequence