Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4683 and Pf6N2E2_4684 are separated by 121 nucleotides Pf6N2E2_4684 and Pf6N2E2_4685 are separated by 75 nucleotides Pf6N2E2_4685 and Pf6N2E2_4686 are separated by 574 nucleotides
Pf6N2E2_4683 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 5,416,991 to 5,417,938
_4683
Pf6N2E2_4684 - hypothetical protein, at 5,418,060 to 5,418,287
_4684
Pf6N2E2_4685 - L-serine dehydratase (EC 4.3.1.17), at 5,418,363 to 5,419,739
_4685
Pf6N2E2_4686 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 5,420,314 to 5,421,417
_4686
Position (kb) 5418
5419
5420 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5417.506 kb on - strand, within Pf6N2E2_4683 at 5417.571 kb on + strand, within Pf6N2E2_4683 at 5417.571 kb on + strand, within Pf6N2E2_4683 at 5417.572 kb on - strand, within Pf6N2E2_4683 at 5417.599 kb on - strand, within Pf6N2E2_4683 at 5417.599 kb on - strand, within Pf6N2E2_4683 at 5417.599 kb on - strand, within Pf6N2E2_4683 at 5417.599 kb on - strand, within Pf6N2E2_4683 at 5417.699 kb on + strand, within Pf6N2E2_4683 at 5417.699 kb on + strand, within Pf6N2E2_4683 at 5417.699 kb on + strand, within Pf6N2E2_4683 at 5417.699 kb on + strand, within Pf6N2E2_4683 at 5417.699 kb on + strand, within Pf6N2E2_4683 at 5417.699 kb on + strand, within Pf6N2E2_4683 at 5417.700 kb on - strand, within Pf6N2E2_4683 at 5417.733 kb on + strand, within Pf6N2E2_4683 at 5417.733 kb on + strand, within Pf6N2E2_4683 at 5417.733 kb on + strand, within Pf6N2E2_4683 at 5417.733 kb on + strand, within Pf6N2E2_4683 at 5417.733 kb on + strand, within Pf6N2E2_4683 at 5417.733 kb on + strand, within Pf6N2E2_4683 at 5417.733 kb on + strand, within Pf6N2E2_4683 at 5417.733 kb on + strand, within Pf6N2E2_4683 at 5417.734 kb on - strand, within Pf6N2E2_4683 at 5417.734 kb on - strand, within Pf6N2E2_4683 at 5417.734 kb on - strand, within Pf6N2E2_4683 at 5417.991 kb on - strand at 5418.065 kb on + strand at 5418.065 kb on + strand at 5418.065 kb on + strand at 5418.065 kb on + strand at 5418.065 kb on + strand at 5418.065 kb on + strand at 5418.065 kb on + strand at 5418.065 kb on + strand at 5418.065 kb on + strand at 5418.065 kb on + strand at 5418.065 kb on + strand at 5418.065 kb on + strand at 5418.066 kb on - strand at 5418.066 kb on - strand at 5418.066 kb on - strand at 5418.066 kb on - strand at 5418.066 kb on - strand at 5418.066 kb on - strand at 5418.066 kb on - strand at 5418.066 kb on - strand at 5418.086 kb on + strand, within Pf6N2E2_4684 at 5418.087 kb on - strand, within Pf6N2E2_4684 at 5418.087 kb on - strand, within Pf6N2E2_4684 at 5418.087 kb on - strand, within Pf6N2E2_4684 at 5418.107 kb on + strand, within Pf6N2E2_4684 at 5418.144 kb on + strand, within Pf6N2E2_4684 at 5418.145 kb on - strand, within Pf6N2E2_4684 at 5418.177 kb on + strand, within Pf6N2E2_4684 at 5418.178 kb on - strand, within Pf6N2E2_4684 at 5418.302 kb on + strand at 5418.303 kb on - strand at 5418.686 kb on - strand, within Pf6N2E2_4685 at 5418.686 kb on - strand, within Pf6N2E2_4685 at 5418.686 kb on - strand, within Pf6N2E2_4685 at 5418.686 kb on - strand, within Pf6N2E2_4685 at 5418.686 kb on - strand, within Pf6N2E2_4685 at 5418.686 kb on - strand, within Pf6N2E2_4685 at 5418.789 kb on + strand, within Pf6N2E2_4685 at 5418.789 kb on + strand, within Pf6N2E2_4685 at 5418.789 kb on + strand, within Pf6N2E2_4685 at 5418.789 kb on + strand, within Pf6N2E2_4685 at 5418.789 kb on + strand, within Pf6N2E2_4685 at 5418.789 kb on + strand, within Pf6N2E2_4685 at 5418.789 kb on + strand, within Pf6N2E2_4685 at 5418.789 kb on + strand, within Pf6N2E2_4685 at 5418.789 kb on + strand, within Pf6N2E2_4685 at 5418.789 kb on + strand, within Pf6N2E2_4685 at 5418.790 kb on - strand, within Pf6N2E2_4685 at 5418.790 kb on - strand, within Pf6N2E2_4685 at 5418.790 kb on - strand, within Pf6N2E2_4685 at 5418.790 kb on - strand, within Pf6N2E2_4685 at 5418.790 kb on - strand, within Pf6N2E2_4685 at 5418.790 kb on - strand, within Pf6N2E2_4685 at 5418.790 kb on - strand, within Pf6N2E2_4685 at 5418.790 kb on - strand, within Pf6N2E2_4685 at 5418.790 kb on - strand, within Pf6N2E2_4685 at 5418.790 kb on - strand, within Pf6N2E2_4685 at 5418.792 kb on + strand, within Pf6N2E2_4685 at 5418.792 kb on + strand, within Pf6N2E2_4685 at 5418.792 kb on + strand, within Pf6N2E2_4685 at 5418.792 kb on + strand, within Pf6N2E2_4685 at 5418.793 kb on - strand, within Pf6N2E2_4685 at 5418.873 kb on + strand, within Pf6N2E2_4685 at 5418.873 kb on + strand, within Pf6N2E2_4685 at 5418.873 kb on + strand, within Pf6N2E2_4685 at 5418.873 kb on + strand, within Pf6N2E2_4685 at 5418.874 kb on - strand, within Pf6N2E2_4685 at 5419.269 kb on + strand, within Pf6N2E2_4685 at 5419.269 kb on + strand, within Pf6N2E2_4685 at 5419.270 kb on - strand, within Pf6N2E2_4685 at 5419.270 kb on - strand, within Pf6N2E2_4685 at 5419.317 kb on + strand, within Pf6N2E2_4685 at 5419.318 kb on - strand, within Pf6N2E2_4685 at 5419.318 kb on - strand, within Pf6N2E2_4685 at 5419.689 kb on + strand at 5419.689 kb on + strand at 5419.689 kb on + strand at 5419.689 kb on + strand at 5419.689 kb on + strand at 5419.690 kb on - strand at 5419.690 kb on - strand at 5419.691 kb on + strand at 5419.691 kb on + strand at 5419.691 kb on + strand at 5419.691 kb on + strand at 5419.691 kb on + strand at 5419.692 kb on - strand at 5419.887 kb on - strand at 5419.887 kb on - strand at 5419.898 kb on + strand at 5419.899 kb on - strand at 5419.899 kb on - strand at 5419.933 kb on + strand at 5419.934 kb on - strand at 5420.017 kb on + strand at 5420.017 kb on + strand at 5420.017 kb on + strand at 5420.018 kb on - strand at 5420.018 kb on - strand at 5420.059 kb on - strand at 5420.130 kb on + strand at 5420.130 kb on + strand at 5420.131 kb on - strand at 5420.131 kb on - strand at 5420.131 kb on - strand at 5420.131 kb on - strand at 5420.131 kb on - strand at 5420.141 kb on + strand at 5420.142 kb on - strand at 5420.171 kb on + strand at 5420.172 kb on - strand at 5420.172 kb on - strand at 5420.172 kb on - strand at 5420.217 kb on + strand at 5420.217 kb on + strand at 5420.217 kb on + strand at 5420.218 kb on - strand at 5420.218 kb on - strand at 5420.218 kb on - strand at 5420.218 kb on - strand at 5420.252 kb on + strand at 5420.307 kb on + strand at 5420.307 kb on + strand at 5420.417 kb on + strand at 5420.426 kb on + strand, within Pf6N2E2_4686 at 5420.459 kb on + strand, within Pf6N2E2_4686 at 5420.459 kb on + strand, within Pf6N2E2_4686 at 5420.459 kb on + strand, within Pf6N2E2_4686 at 5420.459 kb on + strand, within Pf6N2E2_4686 at 5420.460 kb on - strand, within Pf6N2E2_4686 at 5420.460 kb on - strand, within Pf6N2E2_4686 at 5420.460 kb on - strand, within Pf6N2E2_4686 at 5420.460 kb on - strand, within Pf6N2E2_4686 at 5420.461 kb on + strand, within Pf6N2E2_4686 at 5420.462 kb on - strand, within Pf6N2E2_4686 at 5420.562 kb on + strand, within Pf6N2E2_4686 at 5420.599 kb on - strand, within Pf6N2E2_4686
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 5,417,506 - Pf6N2E2_4683 0.54 -0.1 5,417,571 + Pf6N2E2_4683 0.61 +0.9 5,417,571 + Pf6N2E2_4683 0.61 -1.0 5,417,572 - Pf6N2E2_4683 0.61 -0.0 5,417,599 - Pf6N2E2_4683 0.64 -0.1 5,417,599 - Pf6N2E2_4683 0.64 +0.4 5,417,599 - Pf6N2E2_4683 0.64 -0.3 5,417,599 - Pf6N2E2_4683 0.64 -0.5 5,417,699 + Pf6N2E2_4683 0.75 -0.8 5,417,699 + Pf6N2E2_4683 0.75 -1.3 5,417,699 + Pf6N2E2_4683 0.75 -0.4 5,417,699 + Pf6N2E2_4683 0.75 +0.5 5,417,699 + Pf6N2E2_4683 0.75 +0.0 5,417,699 + Pf6N2E2_4683 0.75 +0.4 5,417,700 - Pf6N2E2_4683 0.75 -2.0 5,417,733 + Pf6N2E2_4683 0.78 +0.1 5,417,733 + Pf6N2E2_4683 0.78 +0.6 5,417,733 + Pf6N2E2_4683 0.78 +1.1 5,417,733 + Pf6N2E2_4683 0.78 -0.5 5,417,733 + Pf6N2E2_4683 0.78 -0.2 5,417,733 + Pf6N2E2_4683 0.78 +0.5 5,417,733 + Pf6N2E2_4683 0.78 -0.4 5,417,733 + Pf6N2E2_4683 0.78 -0.8 5,417,734 - Pf6N2E2_4683 0.78 -0.0 5,417,734 - Pf6N2E2_4683 0.78 -0.9 5,417,734 - Pf6N2E2_4683 0.78 -0.5 5,417,991 - -0.6 5,418,065 + -1.2 5,418,065 + +0.8 5,418,065 + -1.0 5,418,065 + -0.1 5,418,065 + +0.1 5,418,065 + +0.3 5,418,065 + -0.2 5,418,065 + -1.0 5,418,065 + -0.4 5,418,065 + -1.0 5,418,065 + +0.9 5,418,065 + -0.6 5,418,066 - -0.5 5,418,066 - -0.6 5,418,066 - -0.5 5,418,066 - -1.3 5,418,066 - +0.8 5,418,066 - +0.4 5,418,066 - +0.4 5,418,066 - -0.6 5,418,086 + Pf6N2E2_4684 0.11 -0.1 5,418,087 - Pf6N2E2_4684 0.12 -0.3 5,418,087 - Pf6N2E2_4684 0.12 +1.4 5,418,087 - Pf6N2E2_4684 0.12 +0.4 5,418,107 + Pf6N2E2_4684 0.21 -1.5 5,418,144 + Pf6N2E2_4684 0.37 -0.4 5,418,145 - Pf6N2E2_4684 0.37 -0.2 5,418,177 + Pf6N2E2_4684 0.51 -0.0 5,418,178 - Pf6N2E2_4684 0.52 +0.7 5,418,302 + -0.7 5,418,303 - +0.2 5,418,686 - Pf6N2E2_4685 0.23 -0.5 5,418,686 - Pf6N2E2_4685 0.23 -1.8 5,418,686 - Pf6N2E2_4685 0.23 +0.1 5,418,686 - Pf6N2E2_4685 0.23 -0.2 5,418,686 - Pf6N2E2_4685 0.23 -0.9 5,418,686 - Pf6N2E2_4685 0.23 +0.0 5,418,789 + Pf6N2E2_4685 0.31 +1.9 5,418,789 + Pf6N2E2_4685 0.31 -0.5 5,418,789 + Pf6N2E2_4685 0.31 -1.3 5,418,789 + Pf6N2E2_4685 0.31 +0.4 5,418,789 + Pf6N2E2_4685 0.31 +0.2 5,418,789 + Pf6N2E2_4685 0.31 -0.1 5,418,789 + Pf6N2E2_4685 0.31 +0.1 5,418,789 + Pf6N2E2_4685 0.31 -0.2 5,418,789 + Pf6N2E2_4685 0.31 -0.1 5,418,789 + Pf6N2E2_4685 0.31 -0.4 5,418,790 - Pf6N2E2_4685 0.31 +0.1 5,418,790 - Pf6N2E2_4685 0.31 +1.2 5,418,790 - Pf6N2E2_4685 0.31 -0.1 5,418,790 - Pf6N2E2_4685 0.31 +0.4 5,418,790 - Pf6N2E2_4685 0.31 -0.2 5,418,790 - Pf6N2E2_4685 0.31 +0.4 5,418,790 - Pf6N2E2_4685 0.31 -0.2 5,418,790 - Pf6N2E2_4685 0.31 +1.4 5,418,790 - Pf6N2E2_4685 0.31 +0.3 5,418,790 - Pf6N2E2_4685 0.31 +0.7 5,418,792 + Pf6N2E2_4685 0.31 -0.0 5,418,792 + Pf6N2E2_4685 0.31 +0.9 5,418,792 + Pf6N2E2_4685 0.31 -0.0 5,418,792 + Pf6N2E2_4685 0.31 +0.7 5,418,793 - Pf6N2E2_4685 0.31 +0.2 5,418,873 + Pf6N2E2_4685 0.37 -0.3 5,418,873 + Pf6N2E2_4685 0.37 -1.2 5,418,873 + Pf6N2E2_4685 0.37 -0.1 5,418,873 + Pf6N2E2_4685 0.37 -0.2 5,418,874 - Pf6N2E2_4685 0.37 -1.2 5,419,269 + Pf6N2E2_4685 0.66 -0.4 5,419,269 + Pf6N2E2_4685 0.66 -1.6 5,419,270 - Pf6N2E2_4685 0.66 +0.5 5,419,270 - Pf6N2E2_4685 0.66 -0.8 5,419,317 + Pf6N2E2_4685 0.69 -0.2 5,419,318 - Pf6N2E2_4685 0.69 -0.1 5,419,318 - Pf6N2E2_4685 0.69 +0.4 5,419,689 + +0.7 5,419,689 + -0.3 5,419,689 + +0.1 5,419,689 + +0.5 5,419,689 + -0.8 5,419,690 - -1.3 5,419,690 - -1.1 5,419,691 + +0.1 5,419,691 + -1.2 5,419,691 + -0.5 5,419,691 + -0.8 5,419,691 + -0.9 5,419,692 - +0.1 5,419,887 - -0.4 5,419,887 - -0.3 5,419,898 + +0.9 5,419,899 - +0.6 5,419,899 - -0.3 5,419,933 + +0.1 5,419,934 - +0.4 5,420,017 + -0.9 5,420,017 + -0.2 5,420,017 + -0.4 5,420,018 - +0.3 5,420,018 - +0.4 5,420,059 - -0.0 5,420,130 + -0.3 5,420,130 + -0.4 5,420,131 - -1.0 5,420,131 - +0.3 5,420,131 - -0.3 5,420,131 - -3.3 5,420,131 - +1.1 5,420,141 + -0.7 5,420,142 - +0.2 5,420,171 + -1.7 5,420,172 - -0.6 5,420,172 - -1.3 5,420,172 - +1.2 5,420,217 + -0.2 5,420,217 + -0.4 5,420,217 + -1.5 5,420,218 - -0.9 5,420,218 - +2.2 5,420,218 - +0.6 5,420,218 - +0.3 5,420,252 + -1.8 5,420,307 + -0.0 5,420,307 + -0.4 5,420,417 + +0.1 5,420,426 + Pf6N2E2_4686 0.10 +0.5 5,420,459 + Pf6N2E2_4686 0.13 +0.2 5,420,459 + Pf6N2E2_4686 0.13 +0.3 5,420,459 + Pf6N2E2_4686 0.13 -1.7 5,420,459 + Pf6N2E2_4686 0.13 +1.5 5,420,460 - Pf6N2E2_4686 0.13 +0.2 5,420,460 - Pf6N2E2_4686 0.13 +0.5 5,420,460 - Pf6N2E2_4686 0.13 -1.0 5,420,460 - Pf6N2E2_4686 0.13 +1.2 5,420,461 + Pf6N2E2_4686 0.13 +1.1 5,420,462 - Pf6N2E2_4686 0.13 -0.8 5,420,562 + Pf6N2E2_4686 0.22 +0.4 5,420,599 - Pf6N2E2_4686 0.26 -0.2
Or see this region's nucleotide sequence