Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4647

Experiment: Choline Lysine 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4645 and Pf6N2E2_4646 are separated by 52 nucleotidesPf6N2E2_4646 and Pf6N2E2_4647 are separated by 81 nucleotidesPf6N2E2_4647 and Pf6N2E2_4648 are separated by 148 nucleotidesPf6N2E2_4648 and Pf6N2E2_4649 are separated by 62 nucleotides Pf6N2E2_4645 - Integral membrane protein, at 5,375,546 to 5,376,379 _4645 Pf6N2E2_4646 - probable membrane protein NMA1176, at 5,376,432 to 5,376,815 _4646 Pf6N2E2_4647 - Membrane-bound lytic murein transglycosylase A precursor (EC 3.2.1.-), at 5,376,897 to 5,378,084 _4647 Pf6N2E2_4648 - Cytochrome c556, at 5,378,233 to 5,378,661 _4648 Pf6N2E2_4649 - Protein yqjC precursor, at 5,378,724 to 5,379,119 _4649 Position (kb) 5376 5377 5378 5379Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 5376.011 kb on + strand, within Pf6N2E2_4645at 5376.011 kb on + strand, within Pf6N2E2_4645at 5376.011 kb on + strand, within Pf6N2E2_4645at 5376.011 kb on + strand, within Pf6N2E2_4645at 5376.011 kb on + strand, within Pf6N2E2_4645at 5376.012 kb on - strand, within Pf6N2E2_4645at 5376.118 kb on - strand, within Pf6N2E2_4645at 5376.192 kb on + strand, within Pf6N2E2_4645at 5376.192 kb on + strand, within Pf6N2E2_4645at 5376.200 kb on + strand, within Pf6N2E2_4645at 5376.299 kb on + strandat 5376.300 kb on - strandat 5376.300 kb on - strandat 5376.300 kb on - strandat 5376.404 kb on + strandat 5376.406 kb on + strandat 5376.407 kb on - strandat 5376.453 kb on - strandat 5376.453 kb on - strandat 5376.477 kb on + strand, within Pf6N2E2_4646at 5376.477 kb on + strand, within Pf6N2E2_4646at 5376.477 kb on + strand, within Pf6N2E2_4646at 5376.477 kb on + strand, within Pf6N2E2_4646at 5376.478 kb on - strand, within Pf6N2E2_4646at 5376.514 kb on - strand, within Pf6N2E2_4646at 5376.527 kb on + strand, within Pf6N2E2_4646at 5376.582 kb on + strand, within Pf6N2E2_4646at 5376.583 kb on - strand, within Pf6N2E2_4646at 5376.583 kb on - strand, within Pf6N2E2_4646at 5376.583 kb on - strand, within Pf6N2E2_4646at 5376.717 kb on + strand, within Pf6N2E2_4646at 5376.717 kb on + strand, within Pf6N2E2_4646at 5376.717 kb on + strand, within Pf6N2E2_4646at 5376.717 kb on + strand, within Pf6N2E2_4646at 5376.717 kb on + strand, within Pf6N2E2_4646at 5376.717 kb on + strand, within Pf6N2E2_4646at 5376.717 kb on + strand, within Pf6N2E2_4646at 5376.717 kb on + strand, within Pf6N2E2_4646at 5376.717 kb on + strand, within Pf6N2E2_4646at 5376.718 kb on - strand, within Pf6N2E2_4646at 5376.718 kb on - strand, within Pf6N2E2_4646at 5376.718 kb on - strand, within Pf6N2E2_4646at 5376.718 kb on - strand, within Pf6N2E2_4646at 5376.718 kb on - strand, within Pf6N2E2_4646at 5376.718 kb on - strand, within Pf6N2E2_4646at 5376.729 kb on + strand, within Pf6N2E2_4646at 5376.730 kb on - strand, within Pf6N2E2_4646at 5376.917 kb on + strandat 5376.918 kb on - strandat 5376.918 kb on - strandat 5376.933 kb on + strandat 5376.933 kb on + strandat 5376.934 kb on - strandat 5376.934 kb on - strandat 5377.155 kb on + strand, within Pf6N2E2_4647at 5377.155 kb on + strand, within Pf6N2E2_4647at 5377.155 kb on + strand, within Pf6N2E2_4647at 5377.155 kb on + strand, within Pf6N2E2_4647at 5377.156 kb on - strand, within Pf6N2E2_4647at 5377.198 kb on - strand, within Pf6N2E2_4647at 5377.255 kb on + strand, within Pf6N2E2_4647at 5377.255 kb on + strand, within Pf6N2E2_4647at 5377.255 kb on + strand, within Pf6N2E2_4647at 5377.255 kb on + strand, within Pf6N2E2_4647at 5377.256 kb on - strand, within Pf6N2E2_4647at 5377.256 kb on - strand, within Pf6N2E2_4647at 5377.256 kb on - strand, within Pf6N2E2_4647at 5377.256 kb on - strand, within Pf6N2E2_4647at 5377.256 kb on - strand, within Pf6N2E2_4647at 5377.286 kb on + strand, within Pf6N2E2_4647at 5377.287 kb on - strand, within Pf6N2E2_4647at 5377.287 kb on - strand, within Pf6N2E2_4647at 5377.555 kb on + strand, within Pf6N2E2_4647at 5377.615 kb on + strand, within Pf6N2E2_4647at 5377.615 kb on + strand, within Pf6N2E2_4647at 5377.616 kb on - strand, within Pf6N2E2_4647at 5377.616 kb on - strand, within Pf6N2E2_4647at 5377.616 kb on - strand, within Pf6N2E2_4647at 5377.660 kb on + strand, within Pf6N2E2_4647at 5377.660 kb on + strand, within Pf6N2E2_4647at 5377.660 kb on + strand, within Pf6N2E2_4647at 5377.660 kb on + strand, within Pf6N2E2_4647at 5377.661 kb on - strand, within Pf6N2E2_4647at 5377.717 kb on + strand, within Pf6N2E2_4647at 5377.717 kb on + strand, within Pf6N2E2_4647at 5377.717 kb on + strand, within Pf6N2E2_4647at 5377.718 kb on - strand, within Pf6N2E2_4647at 5377.768 kb on + strand, within Pf6N2E2_4647at 5377.769 kb on - strand, within Pf6N2E2_4647at 5377.769 kb on - strand, within Pf6N2E2_4647at 5377.800 kb on + strand, within Pf6N2E2_4647at 5377.800 kb on + strand, within Pf6N2E2_4647at 5377.801 kb on - strand, within Pf6N2E2_4647at 5377.801 kb on - strand, within Pf6N2E2_4647at 5377.801 kb on - strand, within Pf6N2E2_4647at 5377.801 kb on - strand, within Pf6N2E2_4647at 5377.960 kb on + strand, within Pf6N2E2_4647at 5377.960 kb on + strand, within Pf6N2E2_4647at 5377.960 kb on + strand, within Pf6N2E2_4647at 5377.960 kb on + strand, within Pf6N2E2_4647at 5377.960 kb on + strand, within Pf6N2E2_4647at 5377.961 kb on - strand, within Pf6N2E2_4647at 5377.961 kb on - strand, within Pf6N2E2_4647at 5377.961 kb on - strand, within Pf6N2E2_4647at 5377.961 kb on - strand, within Pf6N2E2_4647at 5377.961 kb on - strand, within Pf6N2E2_4647at 5377.961 kb on - strand, within Pf6N2E2_4647at 5377.961 kb on - strand, within Pf6N2E2_4647at 5377.961 kb on - strand, within Pf6N2E2_4647at 5378.038 kb on - strandat 5378.147 kb on + strandat 5378.180 kb on + strandat 5378.180 kb on + strandat 5378.181 kb on - strandat 5378.181 kb on - strandat 5378.183 kb on + strandat 5378.184 kb on - strandat 5378.219 kb on + strandat 5378.578 kb on + strand, within Pf6N2E2_4648at 5378.578 kb on + strand, within Pf6N2E2_4648at 5378.578 kb on + strand, within Pf6N2E2_4648at 5378.579 kb on - strand, within Pf6N2E2_4648at 5378.579 kb on - strand, within Pf6N2E2_4648at 5378.579 kb on - strand, within Pf6N2E2_4648at 5378.590 kb on + strand, within Pf6N2E2_4648at 5378.590 kb on + strand, within Pf6N2E2_4648at 5378.591 kb on - strand, within Pf6N2E2_4648at 5378.591 kb on - strand, within Pf6N2E2_4648at 5378.591 kb on - strand, within Pf6N2E2_4648at 5378.591 kb on - strand, within Pf6N2E2_4648at 5378.591 kb on - strand, within Pf6N2E2_4648at 5378.640 kb on + strandat 5378.640 kb on + strandat 5378.641 kb on - strandat 5378.641 kb on - strandat 5378.725 kb on + strandat 5378.725 kb on + strandat 5378.726 kb on - strandat 5379.084 kb on + strandat 5379.084 kb on + strandat 5379.084 kb on + strandat 5379.084 kb on + strandat 5379.084 kb on + strandat 5379.084 kb on + strandat 5379.084 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline Lysine 20 mM
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5,376,011 + Pf6N2E2_4645 0.56 +0.3
5,376,011 + Pf6N2E2_4645 0.56 +0.0
5,376,011 + Pf6N2E2_4645 0.56 -0.7
5,376,011 + Pf6N2E2_4645 0.56 +0.5
5,376,011 + Pf6N2E2_4645 0.56 -1.0
5,376,012 - Pf6N2E2_4645 0.56 -0.6
5,376,118 - Pf6N2E2_4645 0.69 +0.2
5,376,192 + Pf6N2E2_4645 0.77 -0.4
5,376,192 + Pf6N2E2_4645 0.77 +0.5
5,376,200 + Pf6N2E2_4645 0.78 +0.8
5,376,299 + +1.4
5,376,300 - -0.1
5,376,300 - -0.5
5,376,300 - -0.2
5,376,404 + +0.2
5,376,406 + +0.6
5,376,407 - +1.0
5,376,453 - -0.6
5,376,453 - +1.0
5,376,477 + Pf6N2E2_4646 0.12 +0.7
5,376,477 + Pf6N2E2_4646 0.12 -0.2
5,376,477 + Pf6N2E2_4646 0.12 +0.1
5,376,477 + Pf6N2E2_4646 0.12 -0.0
5,376,478 - Pf6N2E2_4646 0.12 +0.1
5,376,514 - Pf6N2E2_4646 0.21 +2.4
5,376,527 + Pf6N2E2_4646 0.25 +0.4
5,376,582 + Pf6N2E2_4646 0.39 -0.5
5,376,583 - Pf6N2E2_4646 0.39 -0.7
5,376,583 - Pf6N2E2_4646 0.39 -1.4
5,376,583 - Pf6N2E2_4646 0.39 -0.5
5,376,717 + Pf6N2E2_4646 0.74 +0.2
5,376,717 + Pf6N2E2_4646 0.74 +0.3
5,376,717 + Pf6N2E2_4646 0.74 -2.7
5,376,717 + Pf6N2E2_4646 0.74 +1.1
5,376,717 + Pf6N2E2_4646 0.74 +0.1
5,376,717 + Pf6N2E2_4646 0.74 -0.5
5,376,717 + Pf6N2E2_4646 0.74 -0.4
5,376,717 + Pf6N2E2_4646 0.74 +0.4
5,376,717 + Pf6N2E2_4646 0.74 +0.3
5,376,718 - Pf6N2E2_4646 0.74 +0.4
5,376,718 - Pf6N2E2_4646 0.74 +0.5
5,376,718 - Pf6N2E2_4646 0.74 +0.2
5,376,718 - Pf6N2E2_4646 0.74 +0.6
5,376,718 - Pf6N2E2_4646 0.74 +0.3
5,376,718 - Pf6N2E2_4646 0.74 +0.6
5,376,729 + Pf6N2E2_4646 0.77 +1.0
5,376,730 - Pf6N2E2_4646 0.78 +1.1
5,376,917 + -1.2
5,376,918 - -0.1
5,376,918 - +0.1
5,376,933 + -1.7
5,376,933 + -0.6
5,376,934 - -2.2
5,376,934 - -1.1
5,377,155 + Pf6N2E2_4647 0.22 -2.1
5,377,155 + Pf6N2E2_4647 0.22 -2.5
5,377,155 + Pf6N2E2_4647 0.22 -2.7
5,377,155 + Pf6N2E2_4647 0.22 -4.5
5,377,156 - Pf6N2E2_4647 0.22 -2.0
5,377,198 - Pf6N2E2_4647 0.25 -3.2
5,377,255 + Pf6N2E2_4647 0.30 -4.0
5,377,255 + Pf6N2E2_4647 0.30 -2.0
5,377,255 + Pf6N2E2_4647 0.30 -2.7
5,377,255 + Pf6N2E2_4647 0.30 -2.2
5,377,256 - Pf6N2E2_4647 0.30 -2.2
5,377,256 - Pf6N2E2_4647 0.30 -2.0
5,377,256 - Pf6N2E2_4647 0.30 -3.0
5,377,256 - Pf6N2E2_4647 0.30 -1.8
5,377,256 - Pf6N2E2_4647 0.30 -1.1
5,377,286 + Pf6N2E2_4647 0.33 -5.0
5,377,287 - Pf6N2E2_4647 0.33 -2.2
5,377,287 - Pf6N2E2_4647 0.33 -2.7
5,377,555 + Pf6N2E2_4647 0.55 -2.2
5,377,615 + Pf6N2E2_4647 0.60 -2.9
5,377,615 + Pf6N2E2_4647 0.60 -4.2
5,377,616 - Pf6N2E2_4647 0.61 -2.2
5,377,616 - Pf6N2E2_4647 0.61 -1.5
5,377,616 - Pf6N2E2_4647 0.61 +0.6
5,377,660 + Pf6N2E2_4647 0.64 -2.5
5,377,660 + Pf6N2E2_4647 0.64 -4.0
5,377,660 + Pf6N2E2_4647 0.64 -3.1
5,377,660 + Pf6N2E2_4647 0.64 -0.2
5,377,661 - Pf6N2E2_4647 0.64 -2.7
5,377,717 + Pf6N2E2_4647 0.69 -0.7
5,377,717 + Pf6N2E2_4647 0.69 -2.8
5,377,717 + Pf6N2E2_4647 0.69 -2.3
5,377,718 - Pf6N2E2_4647 0.69 -4.0
5,377,768 + Pf6N2E2_4647 0.73 -3.5
5,377,769 - Pf6N2E2_4647 0.73 -2.6
5,377,769 - Pf6N2E2_4647 0.73 -2.6
5,377,800 + Pf6N2E2_4647 0.76 -1.5
5,377,800 + Pf6N2E2_4647 0.76 -2.0
5,377,801 - Pf6N2E2_4647 0.76 -3.4
5,377,801 - Pf6N2E2_4647 0.76 -3.7
5,377,801 - Pf6N2E2_4647 0.76 -2.9
5,377,801 - Pf6N2E2_4647 0.76 -3.0
5,377,960 + Pf6N2E2_4647 0.89 -2.1
5,377,960 + Pf6N2E2_4647 0.89 -3.2
5,377,960 + Pf6N2E2_4647 0.89 -4.3
5,377,960 + Pf6N2E2_4647 0.89 -0.1
5,377,960 + Pf6N2E2_4647 0.89 -3.1
5,377,961 - Pf6N2E2_4647 0.90 -0.9
5,377,961 - Pf6N2E2_4647 0.90 -1.7
5,377,961 - Pf6N2E2_4647 0.90 -4.1
5,377,961 - Pf6N2E2_4647 0.90 -1.8
5,377,961 - Pf6N2E2_4647 0.90 -2.1
5,377,961 - Pf6N2E2_4647 0.90 -2.1
5,377,961 - Pf6N2E2_4647 0.90 -3.9
5,377,961 - Pf6N2E2_4647 0.90 -4.1
5,378,038 - -2.4
5,378,147 + -3.4
5,378,180 + -0.6
5,378,180 + -1.4
5,378,181 - +1.3
5,378,181 - +0.1
5,378,183 + -0.7
5,378,184 - +0.4
5,378,219 + -2.8
5,378,578 + Pf6N2E2_4648 0.80 -0.7
5,378,578 + Pf6N2E2_4648 0.80 -0.4
5,378,578 + Pf6N2E2_4648 0.80 +1.1
5,378,579 - Pf6N2E2_4648 0.81 -0.1
5,378,579 - Pf6N2E2_4648 0.81 +0.4
5,378,579 - Pf6N2E2_4648 0.81 -0.2
5,378,590 + Pf6N2E2_4648 0.83 -0.3
5,378,590 + Pf6N2E2_4648 0.83 -0.4
5,378,591 - Pf6N2E2_4648 0.83 +0.5
5,378,591 - Pf6N2E2_4648 0.83 +1.6
5,378,591 - Pf6N2E2_4648 0.83 +1.7
5,378,591 - Pf6N2E2_4648 0.83 -1.1
5,378,591 - Pf6N2E2_4648 0.83 +0.6
5,378,640 + -0.4
5,378,640 + -0.6
5,378,641 - +0.8
5,378,641 - +0.2
5,378,725 + +0.1
5,378,725 + -0.6
5,378,726 - -1.3
5,379,084 + +0.6
5,379,084 + +0.1
5,379,084 + -0.1
5,379,084 + +0.8
5,379,084 + +0.1
5,379,084 + -0.6
5,379,084 + -1.2

Or see this region's nucleotide sequence