Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_462 and Pf6N2E2_463 are separated by 167 nucleotides Pf6N2E2_463 and Pf6N2E2_464 are separated by 4 nucleotides Pf6N2E2_464 and Pf6N2E2_465 are separated by 68 nucleotides Pf6N2E2_465 and Pf6N2E2_466 are separated by 41 nucleotides
Pf6N2E2_462 - Aspartate aminotransferase (EC 2.6.1.1), at 527,302 to 528,486
_462
Pf6N2E2_463 - Transcriptional regulatory protein RstA, at 528,654 to 529,355
_463
Pf6N2E2_464 - Sensory histidine kinase QseC, at 529,360 to 530,661
_464
Pf6N2E2_465 - Probable transmembrane protein, at 530,730 to 531,371
_465
Pf6N2E2_466 - Nicotinamidase (EC 3.5.1.19), at 531,413 to 532,066
_466
Position (kb) 529
530
531 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 528.391 kb on - strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.546 kb on + strand at 528.548 kb on + strand at 528.711 kb on + strand at 528.712 kb on - strand at 528.948 kb on + strand, within Pf6N2E2_463 at 528.948 kb on + strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.949 kb on - strand, within Pf6N2E2_463 at 528.995 kb on + strand, within Pf6N2E2_463 at 529.131 kb on + strand, within Pf6N2E2_463 at 529.132 kb on - strand, within Pf6N2E2_463 at 529.132 kb on - strand, within Pf6N2E2_463 at 529.171 kb on + strand, within Pf6N2E2_463 at 529.171 kb on + strand, within Pf6N2E2_463 at 529.172 kb on - strand, within Pf6N2E2_463 at 529.172 kb on - strand, within Pf6N2E2_463 at 529.172 kb on - strand, within Pf6N2E2_463 at 529.406 kb on - strand at 529.420 kb on + strand at 529.421 kb on - strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.471 kb on + strand at 529.472 kb on - strand at 529.484 kb on - strand at 529.774 kb on + strand, within Pf6N2E2_464 at 529.775 kb on - strand, within Pf6N2E2_464 at 529.775 kb on - strand, within Pf6N2E2_464 at 530.732 kb on - strand at 530.754 kb on - strand at 530.890 kb on + strand, within Pf6N2E2_465 at 530.905 kb on + strand, within Pf6N2E2_465 at 530.905 kb on + strand, within Pf6N2E2_465 at 530.905 kb on + strand, within Pf6N2E2_465 at 530.906 kb on - strand, within Pf6N2E2_465 at 530.906 kb on - strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.965 kb on + strand, within Pf6N2E2_465 at 530.966 kb on - strand, within Pf6N2E2_465 at 530.968 kb on + strand, within Pf6N2E2_465 at 530.968 kb on + strand, within Pf6N2E2_465 at 530.969 kb on - strand, within Pf6N2E2_465 at 530.969 kb on - strand, within Pf6N2E2_465 at 531.239 kb on - strand, within Pf6N2E2_465 at 531.239 kb on - strand, within Pf6N2E2_465 at 531.239 kb on - strand, within Pf6N2E2_465 at 531.401 kb on + strand at 531.401 kb on + strand at 531.401 kb on + strand at 531.401 kb on + strand at 531.401 kb on + strand at 531.404 kb on + strand at 531.404 kb on + strand at 531.405 kb on - strand at 531.405 kb on - strand at 531.434 kb on + strand at 531.435 kb on - strand at 531.435 kb on - strand at 531.615 kb on + strand, within Pf6N2E2_466 at 531.615 kb on + strand, within Pf6N2E2_466 at 531.616 kb on - strand, within Pf6N2E2_466
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 528,391 - +0.6 528,546 + +0.8 528,546 + +0.9 528,546 + +0.2 528,546 + +1.2 528,546 + +0.8 528,548 + +0.1 528,711 + -1.5 528,712 - -1.5 528,948 + Pf6N2E2_463 0.42 +0.8 528,948 + Pf6N2E2_463 0.42 -4.2 528,949 - Pf6N2E2_463 0.42 +1.1 528,949 - Pf6N2E2_463 0.42 -0.0 528,949 - Pf6N2E2_463 0.42 +0.4 528,949 - Pf6N2E2_463 0.42 +1.0 528,949 - Pf6N2E2_463 0.42 -1.2 528,995 + Pf6N2E2_463 0.49 -1.5 529,131 + Pf6N2E2_463 0.68 +0.9 529,132 - Pf6N2E2_463 0.68 -0.7 529,132 - Pf6N2E2_463 0.68 +1.0 529,171 + Pf6N2E2_463 0.74 +0.2 529,171 + Pf6N2E2_463 0.74 -0.9 529,172 - Pf6N2E2_463 0.74 -1.7 529,172 - Pf6N2E2_463 0.74 -0.7 529,172 - Pf6N2E2_463 0.74 -1.3 529,406 - -2.2 529,420 + +0.0 529,421 - +0.9 529,471 + -0.6 529,471 + -1.2 529,471 + -2.2 529,471 + -0.6 529,471 + +0.0 529,471 + -0.6 529,472 - +2.0 529,484 - -1.1 529,774 + Pf6N2E2_464 0.32 -1.5 529,775 - Pf6N2E2_464 0.32 -0.4 529,775 - Pf6N2E2_464 0.32 -2.6 530,732 - +0.4 530,754 - -0.0 530,890 + Pf6N2E2_465 0.25 +0.5 530,905 + Pf6N2E2_465 0.27 -1.3 530,905 + Pf6N2E2_465 0.27 -0.3 530,905 + Pf6N2E2_465 0.27 +0.5 530,906 - Pf6N2E2_465 0.27 +0.3 530,906 - Pf6N2E2_465 0.27 +0.7 530,965 + Pf6N2E2_465 0.37 -0.6 530,965 + Pf6N2E2_465 0.37 -0.4 530,965 + Pf6N2E2_465 0.37 +1.1 530,965 + Pf6N2E2_465 0.37 +0.2 530,966 - Pf6N2E2_465 0.37 -0.7 530,968 + Pf6N2E2_465 0.37 -0.5 530,968 + Pf6N2E2_465 0.37 -0.2 530,969 - Pf6N2E2_465 0.37 -2.8 530,969 - Pf6N2E2_465 0.37 -1.0 531,239 - Pf6N2E2_465 0.79 -1.0 531,239 - Pf6N2E2_465 0.79 +0.5 531,239 - Pf6N2E2_465 0.79 -0.1 531,401 + +0.6 531,401 + -0.4 531,401 + +1.2 531,401 + +0.0 531,401 + -0.7 531,404 + +1.1 531,404 + +0.5 531,405 - -0.9 531,405 - +0.5 531,434 + -0.3 531,435 - +0.1 531,435 - -0.2 531,615 + Pf6N2E2_466 0.31 +0.4 531,615 + Pf6N2E2_466 0.31 +0.4 531,616 - Pf6N2E2_466 0.31 +0.8
Or see this region's nucleotide sequence