Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4590 and Pf6N2E2_4591 are separated by 9 nucleotides Pf6N2E2_4591 and Pf6N2E2_4592 are separated by 144 nucleotides Pf6N2E2_4592 and Pf6N2E2_4593 are separated by 21 nucleotides
Pf6N2E2_4590 - Thiazole biosynthesis protein ThiG, at 5,319,098 to 5,319,892
_4590
Pf6N2E2_4591 - tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33), at 5,319,902 to 5,320,627
_4591
Pf6N2E2_4592 - Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific, at 5,320,772 to 5,321,095
_4592
Pf6N2E2_4593 - Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB, at 5,321,117 to 5,322,250
_4593
Position (kb) 5320
5321
5322 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5319.821 kb on + strand at 5319.852 kb on - strand at 5319.890 kb on + strand at 5319.890 kb on + strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.458 kb on - strand, within Pf6N2E2_4591 at 5320.458 kb on - strand, within Pf6N2E2_4591 at 5320.466 kb on + strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.626 kb on - strand at 5320.690 kb on + strand at 5320.690 kb on + strand at 5320.691 kb on - strand at 5320.691 kb on - strand at 5320.716 kb on + strand at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.881 kb on + strand, within Pf6N2E2_4592 at 5320.881 kb on + strand, within Pf6N2E2_4592 at 5320.881 kb on + strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592 at 5320.931 kb on + strand, within Pf6N2E2_4592 at 5321.115 kb on - strand at 5321.118 kb on + strand at 5321.118 kb on + strand at 5321.118 kb on + strand at 5321.119 kb on - strand at 5321.119 kb on - strand at 5321.119 kb on - strand at 5321.119 kb on - strand at 5321.119 kb on - strand at 5321.388 kb on + strand, within Pf6N2E2_4593 at 5321.388 kb on + strand, within Pf6N2E2_4593 at 5321.388 kb on + strand, within Pf6N2E2_4593 at 5321.389 kb on - strand, within Pf6N2E2_4593 at 5321.420 kb on + strand, within Pf6N2E2_4593 at 5321.421 kb on - strand, within Pf6N2E2_4593 at 5321.496 kb on + strand, within Pf6N2E2_4593 at 5321.496 kb on + strand, within Pf6N2E2_4593 at 5321.497 kb on - strand, within Pf6N2E2_4593 at 5321.506 kb on + strand, within Pf6N2E2_4593 at 5321.550 kb on + strand, within Pf6N2E2_4593
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 5,319,821 + -1.3 5,319,852 - +1.4 5,319,890 + -2.5 5,319,890 + +0.4 5,319,891 - +0.3 5,319,891 - -1.9 5,319,891 - +1.7 5,319,891 - -2.1 5,319,891 - -0.1 5,319,891 - +0.7 5,320,457 + Pf6N2E2_4591 0.76 +0.8 5,320,457 + Pf6N2E2_4591 0.76 +0.8 5,320,457 + Pf6N2E2_4591 0.76 +0.1 5,320,457 + Pf6N2E2_4591 0.76 +0.1 5,320,458 - Pf6N2E2_4591 0.77 +0.0 5,320,458 - Pf6N2E2_4591 0.77 +0.5 5,320,466 + Pf6N2E2_4591 0.78 +0.0 5,320,467 - Pf6N2E2_4591 0.78 +0.6 5,320,467 - Pf6N2E2_4591 0.78 -0.0 5,320,467 - Pf6N2E2_4591 0.78 +0.0 5,320,467 - Pf6N2E2_4591 0.78 -1.5 5,320,467 - Pf6N2E2_4591 0.78 +1.3 5,320,467 - Pf6N2E2_4591 0.78 -0.7 5,320,467 - Pf6N2E2_4591 0.78 +0.0 5,320,626 - -0.4 5,320,690 + -0.0 5,320,690 + -0.5 5,320,691 - +1.1 5,320,691 - +0.7 5,320,716 + -1.6 5,320,836 + Pf6N2E2_4592 0.20 -0.0 5,320,836 + Pf6N2E2_4592 0.20 -1.5 5,320,836 + Pf6N2E2_4592 0.20 -0.1 5,320,836 + Pf6N2E2_4592 0.20 -0.9 5,320,836 + Pf6N2E2_4592 0.20 -1.4 5,320,836 + Pf6N2E2_4592 0.20 -1.0 5,320,837 - Pf6N2E2_4592 0.20 -0.1 5,320,837 - Pf6N2E2_4592 0.20 -0.9 5,320,837 - Pf6N2E2_4592 0.20 +0.3 5,320,837 - Pf6N2E2_4592 0.20 -1.0 5,320,837 - Pf6N2E2_4592 0.20 -2.8 5,320,837 - Pf6N2E2_4592 0.20 -3.2 5,320,837 - Pf6N2E2_4592 0.20 -2.1 5,320,837 - Pf6N2E2_4592 0.20 -2.2 5,320,881 + Pf6N2E2_4592 0.34 -0.8 5,320,881 + Pf6N2E2_4592 0.34 -0.6 5,320,881 + Pf6N2E2_4592 0.34 -1.5 5,320,882 - Pf6N2E2_4592 0.34 -2.0 5,320,882 - Pf6N2E2_4592 0.34 -0.8 5,320,882 - Pf6N2E2_4592 0.34 -0.6 5,320,882 - Pf6N2E2_4592 0.34 +0.7 5,320,931 + Pf6N2E2_4592 0.49 -1.2 5,321,115 - +0.3 5,321,118 + -3.2 5,321,118 + -3.0 5,321,118 + -1.9 5,321,119 - +0.6 5,321,119 - +0.1 5,321,119 - +0.3 5,321,119 - +0.3 5,321,119 - +0.8 5,321,388 + Pf6N2E2_4593 0.24 +0.7 5,321,388 + Pf6N2E2_4593 0.24 -0.7 5,321,388 + Pf6N2E2_4593 0.24 -0.6 5,321,389 - Pf6N2E2_4593 0.24 +1.9 5,321,420 + Pf6N2E2_4593 0.27 -2.1 5,321,421 - Pf6N2E2_4593 0.27 +0.4 5,321,496 + Pf6N2E2_4593 0.33 -1.2 5,321,496 + Pf6N2E2_4593 0.33 -0.9 5,321,497 - Pf6N2E2_4593 0.34 +1.3 5,321,506 + Pf6N2E2_4593 0.34 -0.3 5,321,550 + Pf6N2E2_4593 0.38 +0.9
Or see this region's nucleotide sequence