Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4128 and Pf6N2E2_4129 are separated by 9 nucleotides Pf6N2E2_4129 and Pf6N2E2_4130 are separated by 13 nucleotides Pf6N2E2_4130 and Pf6N2E2_4131 are separated by 115 nucleotides
Pf6N2E2_4128 - hypothetical protein, at 4,807,293 to 4,807,577
_4128
Pf6N2E2_4129 - Coproporphyrinogen III oxidase, aerobic (EC 1.3.3.3), at 4,807,587 to 4,808,501
_4129
Pf6N2E2_4130 - Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25), at 4,808,515 to 4,809,336
_4130
Pf6N2E2_4131 - Sulfate permease, at 4,809,452 to 4,811,020
_4131
Position (kb)
4808
4809
4810 Strain fitness (log2 ratio)
-2
-1
0
1 at 4807.518 kb on + strand, within Pf6N2E2_4128 at 4807.518 kb on + strand, within Pf6N2E2_4128 at 4807.565 kb on + strand at 4808.524 kb on + strand at 4809.385 kb on - strand at 4809.389 kb on + strand at 4809.389 kb on + strand at 4809.453 kb on + strand at 4809.601 kb on - strand at 4809.675 kb on + strand, within Pf6N2E2_4131 at 4809.675 kb on + strand, within Pf6N2E2_4131 at 4809.676 kb on - strand, within Pf6N2E2_4131 at 4809.676 kb on - strand, within Pf6N2E2_4131 at 4809.775 kb on - strand, within Pf6N2E2_4131 at 4809.804 kb on + strand, within Pf6N2E2_4131 at 4809.804 kb on + strand, within Pf6N2E2_4131 at 4809.804 kb on + strand, within Pf6N2E2_4131 at 4809.804 kb on + strand, within Pf6N2E2_4131 at 4809.804 kb on + strand, within Pf6N2E2_4131 at 4809.804 kb on + strand, within Pf6N2E2_4131 at 4809.804 kb on + strand, within Pf6N2E2_4131 at 4809.804 kb on + strand, within Pf6N2E2_4131 at 4809.804 kb on + strand, within Pf6N2E2_4131 at 4809.805 kb on - strand, within Pf6N2E2_4131 at 4809.805 kb on - strand, within Pf6N2E2_4131 at 4809.805 kb on - strand, within Pf6N2E2_4131 at 4809.805 kb on - strand, within Pf6N2E2_4131 at 4809.805 kb on - strand, within Pf6N2E2_4131 at 4809.805 kb on - strand, within Pf6N2E2_4131 at 4809.805 kb on - strand, within Pf6N2E2_4131 at 4809.805 kb on - strand, within Pf6N2E2_4131 at 4809.805 kb on - strand, within Pf6N2E2_4131 at 4809.857 kb on + strand, within Pf6N2E2_4131 at 4809.885 kb on + strand, within Pf6N2E2_4131 at 4809.886 kb on - strand, within Pf6N2E2_4131 at 4810.056 kb on + strand, within Pf6N2E2_4131 at 4810.056 kb on + strand, within Pf6N2E2_4131 at 4810.056 kb on + strand, within Pf6N2E2_4131 at 4810.057 kb on - strand, within Pf6N2E2_4131
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 4,807,518 + Pf6N2E2_4128 0.79 +1.3 4,807,518 + Pf6N2E2_4128 0.79 +0.4 4,807,565 + +0.1 4,808,524 + -0.1 4,809,385 - -0.1 4,809,389 + -0.0 4,809,389 + -0.3 4,809,453 + +0.7 4,809,601 - -0.3 4,809,675 + Pf6N2E2_4131 0.14 +1.3 4,809,675 + Pf6N2E2_4131 0.14 -1.1 4,809,676 - Pf6N2E2_4131 0.14 -1.5 4,809,676 - Pf6N2E2_4131 0.14 +1.3 4,809,775 - Pf6N2E2_4131 0.21 -1.2 4,809,804 + Pf6N2E2_4131 0.22 +0.9 4,809,804 + Pf6N2E2_4131 0.22 +1.0 4,809,804 + Pf6N2E2_4131 0.22 -0.5 4,809,804 + Pf6N2E2_4131 0.22 +0.9 4,809,804 + Pf6N2E2_4131 0.22 -1.0 4,809,804 + Pf6N2E2_4131 0.22 -0.1 4,809,804 + Pf6N2E2_4131 0.22 +0.4 4,809,804 + Pf6N2E2_4131 0.22 -1.8 4,809,804 + Pf6N2E2_4131 0.22 +0.0 4,809,805 - Pf6N2E2_4131 0.22 -0.5 4,809,805 - Pf6N2E2_4131 0.22 +0.6 4,809,805 - Pf6N2E2_4131 0.22 +0.3 4,809,805 - Pf6N2E2_4131 0.22 -0.6 4,809,805 - Pf6N2E2_4131 0.22 -2.5 4,809,805 - Pf6N2E2_4131 0.22 +0.2 4,809,805 - Pf6N2E2_4131 0.22 +0.4 4,809,805 - Pf6N2E2_4131 0.22 +1.6 4,809,805 - Pf6N2E2_4131 0.22 -0.0 4,809,857 + Pf6N2E2_4131 0.26 +1.4 4,809,885 + Pf6N2E2_4131 0.28 +0.9 4,809,886 - Pf6N2E2_4131 0.28 +1.4 4,810,056 + Pf6N2E2_4131 0.38 -0.2 4,810,056 + Pf6N2E2_4131 0.38 +0.1 4,810,056 + Pf6N2E2_4131 0.38 -0.2 4,810,057 - Pf6N2E2_4131 0.39 -0.4
Or see this region's nucleotide sequence