Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4002 and Pf6N2E2_4003 are separated by 3 nucleotides Pf6N2E2_4003 and Pf6N2E2_4004 are separated by 12 nucleotides Pf6N2E2_4004 and Pf6N2E2_4005 overlap by 12 nucleotides Pf6N2E2_4005 and Pf6N2E2_4006 are separated by 50 nucleotides
Pf6N2E2_4002 - Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25), at 4,673,181 to 4,674,170
_4002
Pf6N2E2_4003 - Sulfate transport system permease protein CysW, at 4,674,174 to 4,675,043
_4003
Pf6N2E2_4004 - Sulfate transport system permease protein CysT, at 4,675,056 to 4,675,775
_4004
Pf6N2E2_4005 - hypothetical protein, at 4,675,764 to 4,676,054
_4005
Pf6N2E2_4006 - Sulfate and thiosulfate binding protein CysP, at 4,676,105 to 4,677,118
_4006
Position (kb) 4675
4676 Strain fitness (log2 ratio) -1
0
1
2 at 4674.152 kb on + strand at 4674.175 kb on + strand at 4674.175 kb on + strand at 4674.175 kb on + strand at 4674.175 kb on + strand at 4674.176 kb on - strand at 4674.176 kb on - strand at 4674.176 kb on - strand at 4674.176 kb on - strand at 4674.176 kb on - strand at 4674.176 kb on - strand at 4674.176 kb on - strand at 4674.298 kb on + strand, within Pf6N2E2_4003 at 4674.299 kb on - strand, within Pf6N2E2_4003 at 4674.307 kb on + strand, within Pf6N2E2_4003 at 4674.308 kb on - strand, within Pf6N2E2_4003 at 4674.308 kb on - strand, within Pf6N2E2_4003 at 4674.404 kb on + strand, within Pf6N2E2_4003 at 4674.421 kb on + strand, within Pf6N2E2_4003 at 4674.422 kb on - strand, within Pf6N2E2_4003 at 4674.422 kb on - strand, within Pf6N2E2_4003 at 4674.673 kb on + strand, within Pf6N2E2_4003 at 4674.673 kb on + strand, within Pf6N2E2_4003 at 4674.674 kb on - strand, within Pf6N2E2_4003 at 4674.674 kb on - strand, within Pf6N2E2_4003 at 4674.674 kb on - strand, within Pf6N2E2_4003 at 4674.754 kb on + strand, within Pf6N2E2_4003 at 4674.754 kb on + strand, within Pf6N2E2_4003 at 4674.754 kb on + strand, within Pf6N2E2_4003 at 4674.755 kb on - strand, within Pf6N2E2_4003 at 4674.755 kb on - strand, within Pf6N2E2_4003 at 4674.755 kb on - strand, within Pf6N2E2_4003 at 4674.755 kb on - strand, within Pf6N2E2_4003 at 4674.755 kb on - strand, within Pf6N2E2_4003 at 4674.755 kb on - strand, within Pf6N2E2_4003 at 4674.755 kb on - strand, within Pf6N2E2_4003 at 4675.028 kb on + strand at 4675.154 kb on - strand, within Pf6N2E2_4004 at 4675.159 kb on + strand, within Pf6N2E2_4004 at 4675.160 kb on - strand, within Pf6N2E2_4004 at 4675.160 kb on - strand, within Pf6N2E2_4004 at 4675.243 kb on + strand, within Pf6N2E2_4004 at 4675.244 kb on - strand, within Pf6N2E2_4004 at 4675.465 kb on + strand, within Pf6N2E2_4004 at 4675.465 kb on + strand, within Pf6N2E2_4004 at 4675.465 kb on + strand, within Pf6N2E2_4004 at 4675.466 kb on - strand, within Pf6N2E2_4004 at 4675.522 kb on + strand, within Pf6N2E2_4004 at 4675.522 kb on + strand, within Pf6N2E2_4004 at 4675.541 kb on + strand, within Pf6N2E2_4004 at 4675.541 kb on + strand, within Pf6N2E2_4004 at 4675.542 kb on - strand, within Pf6N2E2_4004 at 4675.615 kb on + strand, within Pf6N2E2_4004 at 4675.615 kb on + strand, within Pf6N2E2_4004 at 4675.615 kb on + strand, within Pf6N2E2_4004 at 4675.620 kb on + strand, within Pf6N2E2_4004 at 4675.621 kb on - strand, within Pf6N2E2_4004 at 4675.672 kb on + strand, within Pf6N2E2_4004 at 4675.673 kb on - strand, within Pf6N2E2_4004 at 4675.788 kb on + strand at 4675.788 kb on + strand at 4675.810 kb on + strand, within Pf6N2E2_4005 at 4675.810 kb on + strand, within Pf6N2E2_4005 at 4675.874 kb on + strand, within Pf6N2E2_4005 at 4675.874 kb on + strand, within Pf6N2E2_4005 at 4675.874 kb on + strand, within Pf6N2E2_4005 at 4675.874 kb on + strand, within Pf6N2E2_4005 at 4675.874 kb on + strand, within Pf6N2E2_4005 at 4675.875 kb on - strand, within Pf6N2E2_4005 at 4675.875 kb on - strand, within Pf6N2E2_4005 at 4675.875 kb on - strand, within Pf6N2E2_4005 at 4675.875 kb on - strand, within Pf6N2E2_4005 at 4675.875 kb on - strand, within Pf6N2E2_4005 at 4676.057 kb on - strand at 4676.057 kb on - strand at 4676.091 kb on - strand at 4676.107 kb on - strand at 4676.107 kb on - strand at 4676.118 kb on + strand at 4676.118 kb on + strand at 4676.118 kb on + strand at 4676.118 kb on + strand at 4676.119 kb on + strand at 4676.119 kb on - strand at 4676.119 kb on - strand at 4676.229 kb on + strand, within Pf6N2E2_4006 at 4676.229 kb on + strand, within Pf6N2E2_4006 at 4676.229 kb on + strand, within Pf6N2E2_4006 at 4676.263 kb on - strand, within Pf6N2E2_4006 at 4676.301 kb on + strand, within Pf6N2E2_4006 at 4676.301 kb on + strand, within Pf6N2E2_4006 at 4676.301 kb on + strand, within Pf6N2E2_4006 at 4676.301 kb on + strand, within Pf6N2E2_4006 at 4676.302 kb on - strand, within Pf6N2E2_4006 at 4676.302 kb on - strand, within Pf6N2E2_4006 at 4676.316 kb on + strand, within Pf6N2E2_4006 at 4676.317 kb on - strand, within Pf6N2E2_4006 at 4676.509 kb on + strand, within Pf6N2E2_4006 at 4676.510 kb on - strand, within Pf6N2E2_4006 at 4676.510 kb on - strand, within Pf6N2E2_4006 at 4676.571 kb on + strand, within Pf6N2E2_4006 at 4676.572 kb on - strand, within Pf6N2E2_4006 at 4676.616 kb on + strand, within Pf6N2E2_4006 at 4676.766 kb on + strand, within Pf6N2E2_4006 at 4676.766 kb on + strand, within Pf6N2E2_4006 at 4676.766 kb on + strand, within Pf6N2E2_4006 at 4676.767 kb on - strand, within Pf6N2E2_4006 at 4676.767 kb on - strand, within Pf6N2E2_4006 at 4676.767 kb on - strand, within Pf6N2E2_4006 at 4676.767 kb on - strand, within Pf6N2E2_4006 at 4676.773 kb on + strand, within Pf6N2E2_4006 at 4676.773 kb on + strand, within Pf6N2E2_4006 at 4676.773 kb on + strand, within Pf6N2E2_4006 at 4676.773 kb on + strand, within Pf6N2E2_4006 at 4676.773 kb on + strand, within Pf6N2E2_4006 at 4676.774 kb on - strand, within Pf6N2E2_4006
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 4,674,152 + -0.8 4,674,175 + -0.4 4,674,175 + -0.3 4,674,175 + +0.6 4,674,175 + -0.2 4,674,176 - +0.7 4,674,176 - -1.7 4,674,176 - +0.7 4,674,176 - -0.2 4,674,176 - -0.2 4,674,176 - +0.2 4,674,176 - -0.3 4,674,298 + Pf6N2E2_4003 0.14 -0.5 4,674,299 - Pf6N2E2_4003 0.14 +0.7 4,674,307 + Pf6N2E2_4003 0.15 -0.4 4,674,308 - Pf6N2E2_4003 0.15 +0.4 4,674,308 - Pf6N2E2_4003 0.15 +0.6 4,674,404 + Pf6N2E2_4003 0.26 +0.7 4,674,421 + Pf6N2E2_4003 0.28 +0.3 4,674,422 - Pf6N2E2_4003 0.29 -0.2 4,674,422 - Pf6N2E2_4003 0.29 +0.3 4,674,673 + Pf6N2E2_4003 0.57 -0.1 4,674,673 + Pf6N2E2_4003 0.57 +0.5 4,674,674 - Pf6N2E2_4003 0.57 +1.0 4,674,674 - Pf6N2E2_4003 0.57 -0.8 4,674,674 - Pf6N2E2_4003 0.57 +0.6 4,674,754 + Pf6N2E2_4003 0.67 +1.3 4,674,754 + Pf6N2E2_4003 0.67 +1.7 4,674,754 + Pf6N2E2_4003 0.67 +0.2 4,674,755 - Pf6N2E2_4003 0.67 -0.3 4,674,755 - Pf6N2E2_4003 0.67 -0.8 4,674,755 - Pf6N2E2_4003 0.67 +0.3 4,674,755 - Pf6N2E2_4003 0.67 -0.2 4,674,755 - Pf6N2E2_4003 0.67 +0.9 4,674,755 - Pf6N2E2_4003 0.67 -0.9 4,674,755 - Pf6N2E2_4003 0.67 -0.5 4,675,028 + -0.4 4,675,154 - Pf6N2E2_4004 0.14 +0.2 4,675,159 + Pf6N2E2_4004 0.14 +0.9 4,675,160 - Pf6N2E2_4004 0.14 +1.7 4,675,160 - Pf6N2E2_4004 0.14 -0.0 4,675,243 + Pf6N2E2_4004 0.26 +0.2 4,675,244 - Pf6N2E2_4004 0.26 -0.3 4,675,465 + Pf6N2E2_4004 0.57 +0.9 4,675,465 + Pf6N2E2_4004 0.57 +0.2 4,675,465 + Pf6N2E2_4004 0.57 +0.6 4,675,466 - Pf6N2E2_4004 0.57 +1.1 4,675,522 + Pf6N2E2_4004 0.65 +0.1 4,675,522 + Pf6N2E2_4004 0.65 +1.3 4,675,541 + Pf6N2E2_4004 0.67 -0.8 4,675,541 + Pf6N2E2_4004 0.67 +0.0 4,675,542 - Pf6N2E2_4004 0.68 +0.8 4,675,615 + Pf6N2E2_4004 0.78 +1.3 4,675,615 + Pf6N2E2_4004 0.78 -0.4 4,675,615 + Pf6N2E2_4004 0.78 +0.8 4,675,620 + Pf6N2E2_4004 0.78 +0.4 4,675,621 - Pf6N2E2_4004 0.78 +0.6 4,675,672 + Pf6N2E2_4004 0.86 -0.7 4,675,673 - Pf6N2E2_4004 0.86 -0.1 4,675,788 + +0.3 4,675,788 + -0.7 4,675,810 + Pf6N2E2_4005 0.16 -0.7 4,675,810 + Pf6N2E2_4005 0.16 +0.0 4,675,874 + Pf6N2E2_4005 0.38 +0.3 4,675,874 + Pf6N2E2_4005 0.38 +0.7 4,675,874 + Pf6N2E2_4005 0.38 -0.5 4,675,874 + Pf6N2E2_4005 0.38 -0.2 4,675,874 + Pf6N2E2_4005 0.38 -0.5 4,675,875 - Pf6N2E2_4005 0.38 +1.2 4,675,875 - Pf6N2E2_4005 0.38 +0.4 4,675,875 - Pf6N2E2_4005 0.38 +0.4 4,675,875 - Pf6N2E2_4005 0.38 -0.4 4,675,875 - Pf6N2E2_4005 0.38 +0.6 4,676,057 - +0.4 4,676,057 - +0.0 4,676,091 - +0.1 4,676,107 - -1.6 4,676,107 - -0.3 4,676,118 + -0.9 4,676,118 + -0.9 4,676,118 + -0.8 4,676,118 + -0.7 4,676,119 + +0.0 4,676,119 - -0.4 4,676,119 - +1.9 4,676,229 + Pf6N2E2_4006 0.12 +0.2 4,676,229 + Pf6N2E2_4006 0.12 +1.0 4,676,229 + Pf6N2E2_4006 0.12 +0.2 4,676,263 - Pf6N2E2_4006 0.16 -1.0 4,676,301 + Pf6N2E2_4006 0.19 +0.4 4,676,301 + Pf6N2E2_4006 0.19 -0.1 4,676,301 + Pf6N2E2_4006 0.19 +0.7 4,676,301 + Pf6N2E2_4006 0.19 +0.3 4,676,302 - Pf6N2E2_4006 0.19 +0.8 4,676,302 - Pf6N2E2_4006 0.19 +0.4 4,676,316 + Pf6N2E2_4006 0.21 -0.1 4,676,317 - Pf6N2E2_4006 0.21 +0.9 4,676,509 + Pf6N2E2_4006 0.40 +0.1 4,676,510 - Pf6N2E2_4006 0.40 -0.4 4,676,510 - Pf6N2E2_4006 0.40 -0.4 4,676,571 + Pf6N2E2_4006 0.46 +0.0 4,676,572 - Pf6N2E2_4006 0.46 +0.2 4,676,616 + Pf6N2E2_4006 0.50 -0.0 4,676,766 + Pf6N2E2_4006 0.65 +0.2 4,676,766 + Pf6N2E2_4006 0.65 -0.6 4,676,766 + Pf6N2E2_4006 0.65 +0.1 4,676,767 - Pf6N2E2_4006 0.65 -0.1 4,676,767 - Pf6N2E2_4006 0.65 +0.8 4,676,767 - Pf6N2E2_4006 0.65 -0.3 4,676,767 - Pf6N2E2_4006 0.65 +0.3 4,676,773 + Pf6N2E2_4006 0.66 +0.1 4,676,773 + Pf6N2E2_4006 0.66 +1.2 4,676,773 + Pf6N2E2_4006 0.66 +0.5 4,676,773 + Pf6N2E2_4006 0.66 -0.2 4,676,773 + Pf6N2E2_4006 0.66 -1.3 4,676,774 - Pf6N2E2_4006 0.66 -1.7
Or see this region's nucleotide sequence