Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3695 and Pf6N2E2_3696 are separated by 159 nucleotides Pf6N2E2_3696 and Pf6N2E2_3697 are separated by 133 nucleotides Pf6N2E2_3697 and Pf6N2E2_3698 are separated by 246 nucleotides Pf6N2E2_3698 and Pf6N2E2_3699 are separated by 113 nucleotides
Pf6N2E2_3695 - Helix-turn-helix motif, at 4,311,737 to 4,312,138
_3695
Pf6N2E2_3696 - hypothetical protein, at 4,312,298 to 4,312,576
_3696
Pf6N2E2_3697 - Transcriptional regulator, LysR family, at 4,312,710 to 4,313,576
_3697
Pf6N2E2_3698 - Nicotinamidase family protein YcaC, at 4,313,823 to 4,314,449
_3698
Pf6N2E2_3699 - Thioredoxin, at 4,314,563 to 4,314,985
_3699
Position (kb)
4312
4313
4314 Strain fitness (log2 ratio)
-2
-1
0
1 at 4311.726 kb on - strand at 4311.821 kb on + strand, within Pf6N2E2_3695 at 4312.056 kb on + strand, within Pf6N2E2_3695 at 4312.056 kb on + strand, within Pf6N2E2_3695 at 4312.056 kb on + strand, within Pf6N2E2_3695 at 4312.056 kb on + strand, within Pf6N2E2_3695 at 4312.057 kb on - strand, within Pf6N2E2_3695 at 4312.169 kb on + strand at 4312.170 kb on - strand at 4312.175 kb on + strand at 4312.175 kb on + strand at 4312.175 kb on + strand at 4312.175 kb on + strand at 4312.175 kb on + strand at 4312.175 kb on + strand at 4312.175 kb on + strand at 4312.175 kb on + strand at 4312.175 kb on + strand at 4312.176 kb on - strand at 4312.176 kb on - strand at 4312.176 kb on - strand at 4312.204 kb on + strand at 4312.238 kb on + strand at 4312.239 kb on - strand at 4312.259 kb on - strand at 4312.309 kb on + strand at 4312.310 kb on - strand at 4312.464 kb on + strand, within Pf6N2E2_3696 at 4312.600 kb on + strand at 4312.600 kb on + strand at 4312.600 kb on + strand at 4312.600 kb on + strand at 4312.600 kb on + strand at 4312.601 kb on - strand at 4312.601 kb on - strand at 4312.601 kb on - strand at 4312.601 kb on - strand at 4312.761 kb on - strand at 4313.015 kb on - strand, within Pf6N2E2_3697 at 4313.015 kb on - strand, within Pf6N2E2_3697 at 4313.085 kb on + strand, within Pf6N2E2_3697 at 4313.099 kb on - strand, within Pf6N2E2_3697 at 4313.129 kb on + strand, within Pf6N2E2_3697 at 4313.129 kb on + strand, within Pf6N2E2_3697 at 4313.129 kb on + strand, within Pf6N2E2_3697 at 4313.130 kb on - strand, within Pf6N2E2_3697 at 4313.130 kb on - strand, within Pf6N2E2_3697 at 4313.303 kb on + strand, within Pf6N2E2_3697 at 4313.303 kb on + strand, within Pf6N2E2_3697 at 4313.303 kb on + strand, within Pf6N2E2_3697 at 4313.304 kb on - strand, within Pf6N2E2_3697 at 4313.304 kb on - strand, within Pf6N2E2_3697 at 4313.419 kb on + strand, within Pf6N2E2_3697 at 4313.422 kb on + strand, within Pf6N2E2_3697 at 4313.423 kb on - strand, within Pf6N2E2_3697 at 4313.423 kb on - strand, within Pf6N2E2_3697 at 4313.590 kb on + strand at 4313.590 kb on + strand at 4313.591 kb on - strand at 4313.591 kb on - strand at 4313.593 kb on + strand at 4313.594 kb on - strand at 4313.594 kb on - strand at 4313.594 kb on - strand at 4313.807 kb on + strand at 4313.808 kb on - strand at 4313.808 kb on - strand at 4313.817 kb on + strand at 4313.817 kb on + strand at 4313.835 kb on + strand at 4313.835 kb on + strand at 4313.835 kb on + strand at 4313.836 kb on - strand at 4313.856 kb on - strand at 4313.856 kb on - strand at 4313.856 kb on - strand at 4313.968 kb on - strand, within Pf6N2E2_3698 at 4313.968 kb on - strand, within Pf6N2E2_3698 at 4314.063 kb on + strand, within Pf6N2E2_3698 at 4314.063 kb on + strand, within Pf6N2E2_3698 at 4314.063 kb on + strand, within Pf6N2E2_3698 at 4314.064 kb on - strand, within Pf6N2E2_3698 at 4314.423 kb on + strand at 4314.424 kb on - strand at 4314.517 kb on + strand at 4314.517 kb on + strand at 4314.517 kb on + strand at 4314.517 kb on + strand at 4314.517 kb on + strand at 4314.518 kb on - strand at 4314.518 kb on - strand at 4314.518 kb on - strand at 4314.518 kb on - strand at 4314.518 kb on - strand at 4314.518 kb on - strand at 4314.518 kb on - strand at 4314.518 kb on - strand at 4314.519 kb on + strand at 4314.519 kb on + strand at 4314.520 kb on - strand at 4314.520 kb on - strand at 4314.520 kb on - strand at 4314.521 kb on + strand at 4314.521 kb on + strand at 4314.521 kb on + strand at 4314.521 kb on + strand at 4314.521 kb on + strand at 4314.521 kb on + strand at 4314.521 kb on + strand at 4314.521 kb on + strand at 4314.521 kb on + strand at 4314.521 kb on + strand at 4314.521 kb on + strand at 4314.521 kb on + strand at 4314.521 kb on + strand at 4314.522 kb on - strand at 4314.522 kb on - strand at 4314.522 kb on - strand at 4314.522 kb on - strand at 4314.522 kb on - strand at 4314.522 kb on - strand at 4314.522 kb on - strand at 4314.522 kb on - strand at 4314.522 kb on - strand at 4314.522 kb on - strand at 4314.522 kb on - strand at 4314.522 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 4,311,726 - +0.8 4,311,821 + Pf6N2E2_3695 0.21 +0.4 4,312,056 + Pf6N2E2_3695 0.79 +0.1 4,312,056 + Pf6N2E2_3695 0.79 +0.1 4,312,056 + Pf6N2E2_3695 0.79 -0.4 4,312,056 + Pf6N2E2_3695 0.79 +0.7 4,312,057 - Pf6N2E2_3695 0.80 -1.5 4,312,169 + -0.4 4,312,170 - +0.8 4,312,175 + -1.4 4,312,175 + -0.5 4,312,175 + +0.5 4,312,175 + +0.5 4,312,175 + -1.0 4,312,175 + +0.3 4,312,175 + +1.4 4,312,175 + +0.4 4,312,175 + +0.5 4,312,176 - -0.1 4,312,176 - +0.0 4,312,176 - +0.7 4,312,204 + -0.5 4,312,238 + +0.1 4,312,239 - -0.8 4,312,259 - +0.1 4,312,309 + -0.7 4,312,310 - +1.2 4,312,464 + Pf6N2E2_3696 0.59 -0.2 4,312,600 + +0.5 4,312,600 + +0.1 4,312,600 + -0.8 4,312,600 + +0.1 4,312,600 + -0.3 4,312,601 - -0.1 4,312,601 - -0.0 4,312,601 - +0.2 4,312,601 - -0.0 4,312,761 - +0.9 4,313,015 - Pf6N2E2_3697 0.35 +0.8 4,313,015 - Pf6N2E2_3697 0.35 +0.4 4,313,085 + Pf6N2E2_3697 0.43 +0.7 4,313,099 - Pf6N2E2_3697 0.45 -0.9 4,313,129 + Pf6N2E2_3697 0.48 -0.1 4,313,129 + Pf6N2E2_3697 0.48 +0.3 4,313,129 + Pf6N2E2_3697 0.48 -0.5 4,313,130 - Pf6N2E2_3697 0.48 +0.8 4,313,130 - Pf6N2E2_3697 0.48 +0.8 4,313,303 + Pf6N2E2_3697 0.68 -1.1 4,313,303 + Pf6N2E2_3697 0.68 -0.8 4,313,303 + Pf6N2E2_3697 0.68 +0.5 4,313,304 - Pf6N2E2_3697 0.69 +0.4 4,313,304 - Pf6N2E2_3697 0.69 +0.8 4,313,419 + Pf6N2E2_3697 0.82 +0.4 4,313,422 + Pf6N2E2_3697 0.82 -1.3 4,313,423 - Pf6N2E2_3697 0.82 -1.8 4,313,423 - Pf6N2E2_3697 0.82 +0.3 4,313,590 + +0.1 4,313,590 + +1.4 4,313,591 - +0.3 4,313,591 - -1.2 4,313,593 + +1.1 4,313,594 - +0.3 4,313,594 - +0.3 4,313,594 - -0.3 4,313,807 + -0.0 4,313,808 - -0.7 4,313,808 - +0.8 4,313,817 + +0.6 4,313,817 + -0.1 4,313,835 + +0.1 4,313,835 + +0.7 4,313,835 + -0.3 4,313,836 - +0.1 4,313,856 - -0.3 4,313,856 - -0.5 4,313,856 - +0.7 4,313,968 - Pf6N2E2_3698 0.23 -0.2 4,313,968 - Pf6N2E2_3698 0.23 +0.6 4,314,063 + Pf6N2E2_3698 0.38 +0.0 4,314,063 + Pf6N2E2_3698 0.38 -0.1 4,314,063 + Pf6N2E2_3698 0.38 -1.0 4,314,064 - Pf6N2E2_3698 0.38 -0.7 4,314,423 + -1.0 4,314,424 - +0.6 4,314,517 + +0.3 4,314,517 + -0.5 4,314,517 + -0.4 4,314,517 + +0.2 4,314,517 + +0.4 4,314,518 - +0.4 4,314,518 - +0.4 4,314,518 - +0.0 4,314,518 - -0.3 4,314,518 - -0.3 4,314,518 - +0.6 4,314,518 - -0.8 4,314,518 - -1.1 4,314,519 + -1.2 4,314,519 + -0.5 4,314,520 - +0.3 4,314,520 - +0.1 4,314,520 - -0.1 4,314,521 + +0.7 4,314,521 + +0.1 4,314,521 + +0.8 4,314,521 + +1.0 4,314,521 + -2.2 4,314,521 + +1.3 4,314,521 + +0.4 4,314,521 + +1.2 4,314,521 + +0.0 4,314,521 + +0.1 4,314,521 + +0.7 4,314,521 + +0.7 4,314,521 + -0.6 4,314,522 - +0.4 4,314,522 - -0.7 4,314,522 - +0.7 4,314,522 - +0.4 4,314,522 - +0.5 4,314,522 - +0.5 4,314,522 - +0.0 4,314,522 - -1.1 4,314,522 - -0.1 4,314,522 - +0.1 4,314,522 - +1.0 4,314,522 - +0.1
Or see this region's nucleotide sequence