Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3619

Experiment: Choline Lysine 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3617 and Pf6N2E2_3618 are separated by 3 nucleotidesPf6N2E2_3618 and Pf6N2E2_3619 overlap by 13 nucleotidesPf6N2E2_3619 and Pf6N2E2_3620 are separated by 63 nucleotides Pf6N2E2_3617 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 4,212,391 to 4,213,818 _3617 Pf6N2E2_3618 - TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 4,213,822 to 4,214,289 _3618 Pf6N2E2_3619 - NAD(P)HX epimerase / NAD(P)HX dehydratase, at 4,214,277 to 4,215,776 _3619 Pf6N2E2_3620 - Epoxyqueuosine (oQ) reductase QueG, at 4,215,840 to 4,216,919 _3620 Position (kb) 4214 4215 4216Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4213.804 kb on - strandat 4213.824 kb on - strandat 4214.271 kb on - strandat 4214.578 kb on + strand, within Pf6N2E2_3619at 4214.579 kb on - strand, within Pf6N2E2_3619at 4214.743 kb on - strand, within Pf6N2E2_3619at 4214.743 kb on - strand, within Pf6N2E2_3619at 4214.743 kb on - strand, within Pf6N2E2_3619at 4214.743 kb on - strand, within Pf6N2E2_3619at 4214.743 kb on - strand, within Pf6N2E2_3619at 4214.916 kb on + strand, within Pf6N2E2_3619at 4214.916 kb on + strand, within Pf6N2E2_3619at 4215.087 kb on - strand, within Pf6N2E2_3619at 4215.132 kb on - strand, within Pf6N2E2_3619at 4215.132 kb on - strand, within Pf6N2E2_3619at 4215.132 kb on - strand, within Pf6N2E2_3619at 4215.132 kb on - strand, within Pf6N2E2_3619at 4215.132 kb on - strand, within Pf6N2E2_3619at 4215.132 kb on - strand, within Pf6N2E2_3619at 4215.350 kb on - strand, within Pf6N2E2_3619at 4215.350 kb on - strand, within Pf6N2E2_3619at 4215.350 kb on - strand, within Pf6N2E2_3619at 4215.350 kb on - strand, within Pf6N2E2_3619at 4215.350 kb on - strand, within Pf6N2E2_3619at 4215.350 kb on - strand, within Pf6N2E2_3619at 4215.350 kb on - strand, within Pf6N2E2_3619at 4215.350 kb on - strand, within Pf6N2E2_3619at 4215.740 kb on - strandat 4215.740 kb on - strandat 4215.740 kb on - strandat 4215.740 kb on - strandat 4215.765 kb on - strandat 4215.765 kb on - strandat 4215.765 kb on - strandat 4215.808 kb on - strandat 4215.808 kb on - strandat 4215.808 kb on - strandat 4215.809 kb on + strandat 4215.809 kb on + strandat 4215.809 kb on + strandat 4215.810 kb on - strandat 4215.810 kb on - strandat 4215.810 kb on - strandat 4215.810 kb on - strandat 4215.810 kb on - strandat 4216.002 kb on + strand, within Pf6N2E2_3620at 4216.002 kb on + strand, within Pf6N2E2_3620at 4216.002 kb on + strand, within Pf6N2E2_3620at 4216.003 kb on - strand, within Pf6N2E2_3620at 4216.003 kb on - strand, within Pf6N2E2_3620at 4216.003 kb on - strand, within Pf6N2E2_3620at 4216.003 kb on - strand, within Pf6N2E2_3620at 4216.087 kb on - strand, within Pf6N2E2_3620at 4216.087 kb on - strand, within Pf6N2E2_3620at 4216.087 kb on - strand, within Pf6N2E2_3620at 4216.140 kb on + strand, within Pf6N2E2_3620at 4216.140 kb on + strand, within Pf6N2E2_3620at 4216.140 kb on + strand, within Pf6N2E2_3620at 4216.141 kb on - strand, within Pf6N2E2_3620at 4216.141 kb on - strand, within Pf6N2E2_3620at 4216.141 kb on - strand, within Pf6N2E2_3620at 4216.152 kb on + strand, within Pf6N2E2_3620at 4216.170 kb on + strand, within Pf6N2E2_3620at 4216.317 kb on - strand, within Pf6N2E2_3620at 4216.353 kb on + strand, within Pf6N2E2_3620at 4216.353 kb on + strand, within Pf6N2E2_3620at 4216.353 kb on + strand, within Pf6N2E2_3620at 4216.354 kb on - strand, within Pf6N2E2_3620at 4216.457 kb on + strand, within Pf6N2E2_3620at 4216.457 kb on + strand, within Pf6N2E2_3620at 4216.457 kb on + strand, within Pf6N2E2_3620at 4216.458 kb on - strand, within Pf6N2E2_3620at 4216.479 kb on + strand, within Pf6N2E2_3620at 4216.479 kb on + strand, within Pf6N2E2_3620at 4216.480 kb on - strand, within Pf6N2E2_3620

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline Lysine 20 mM
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4,213,804 - -1.0
4,213,824 - -2.8
4,214,271 - -2.0
4,214,578 + Pf6N2E2_3619 0.20 +0.1
4,214,579 - Pf6N2E2_3619 0.20 +2.1
4,214,743 - Pf6N2E2_3619 0.31 +1.4
4,214,743 - Pf6N2E2_3619 0.31 +0.9
4,214,743 - Pf6N2E2_3619 0.31 +1.5
4,214,743 - Pf6N2E2_3619 0.31 +1.7
4,214,743 - Pf6N2E2_3619 0.31 -0.6
4,214,916 + Pf6N2E2_3619 0.43 +0.9
4,214,916 + Pf6N2E2_3619 0.43 -1.7
4,215,087 - Pf6N2E2_3619 0.54 +1.8
4,215,132 - Pf6N2E2_3619 0.57 +0.9
4,215,132 - Pf6N2E2_3619 0.57 +1.5
4,215,132 - Pf6N2E2_3619 0.57 +0.1
4,215,132 - Pf6N2E2_3619 0.57 +2.2
4,215,132 - Pf6N2E2_3619 0.57 +1.8
4,215,132 - Pf6N2E2_3619 0.57 +1.6
4,215,350 - Pf6N2E2_3619 0.72 +1.5
4,215,350 - Pf6N2E2_3619 0.72 +0.7
4,215,350 - Pf6N2E2_3619 0.72 +1.4
4,215,350 - Pf6N2E2_3619 0.72 +1.2
4,215,350 - Pf6N2E2_3619 0.72 +1.0
4,215,350 - Pf6N2E2_3619 0.72 +1.9
4,215,350 - Pf6N2E2_3619 0.72 +1.4
4,215,350 - Pf6N2E2_3619 0.72 +1.6
4,215,740 - +1.2
4,215,740 - -0.7
4,215,740 - +0.1
4,215,740 - +0.7
4,215,765 - -0.2
4,215,765 - +0.8
4,215,765 - +0.2
4,215,808 - +1.8
4,215,808 - +2.4
4,215,808 - +2.6
4,215,809 + -0.2
4,215,809 + +0.1
4,215,809 + +0.8
4,215,810 - +1.9
4,215,810 - +1.6
4,215,810 - +2.2
4,215,810 - +2.9
4,215,810 - +2.0
4,216,002 + Pf6N2E2_3620 0.15 -0.7
4,216,002 + Pf6N2E2_3620 0.15 -1.5
4,216,002 + Pf6N2E2_3620 0.15 +0.5
4,216,003 - Pf6N2E2_3620 0.15 +2.1
4,216,003 - Pf6N2E2_3620 0.15 +1.3
4,216,003 - Pf6N2E2_3620 0.15 +1.4
4,216,003 - Pf6N2E2_3620 0.15 +1.2
4,216,087 - Pf6N2E2_3620 0.23 +1.5
4,216,087 - Pf6N2E2_3620 0.23 +1.6
4,216,087 - Pf6N2E2_3620 0.23 +1.7
4,216,140 + Pf6N2E2_3620 0.28 +0.5
4,216,140 + Pf6N2E2_3620 0.28 +0.3
4,216,140 + Pf6N2E2_3620 0.28 +0.0
4,216,141 - Pf6N2E2_3620 0.28 +2.9
4,216,141 - Pf6N2E2_3620 0.28 +2.0
4,216,141 - Pf6N2E2_3620 0.28 +0.6
4,216,152 + Pf6N2E2_3620 0.29 +0.7
4,216,170 + Pf6N2E2_3620 0.31 +0.3
4,216,317 - Pf6N2E2_3620 0.44 +2.0
4,216,353 + Pf6N2E2_3620 0.47 +0.2
4,216,353 + Pf6N2E2_3620 0.47 -0.5
4,216,353 + Pf6N2E2_3620 0.47 -0.7
4,216,354 - Pf6N2E2_3620 0.48 +0.0
4,216,457 + Pf6N2E2_3620 0.57 +0.4
4,216,457 + Pf6N2E2_3620 0.57 +0.5
4,216,457 + Pf6N2E2_3620 0.57 +0.5
4,216,458 - Pf6N2E2_3620 0.57 +1.6
4,216,479 + Pf6N2E2_3620 0.59 -0.1
4,216,479 + Pf6N2E2_3620 0.59 +1.4
4,216,480 - Pf6N2E2_3620 0.59 +2.2

Or see this region's nucleotide sequence