Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3617 and Pf6N2E2_3618 are separated by 3 nucleotides Pf6N2E2_3618 and Pf6N2E2_3619 overlap by 13 nucleotides Pf6N2E2_3619 and Pf6N2E2_3620 are separated by 63 nucleotides
Pf6N2E2_3617 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 4,212,391 to 4,213,818
_3617
Pf6N2E2_3618 - TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 4,213,822 to 4,214,289
_3618
Pf6N2E2_3619 - NAD(P)HX epimerase / NAD(P)HX dehydratase, at 4,214,277 to 4,215,776
_3619
Pf6N2E2_3620 - Epoxyqueuosine (oQ) reductase QueG, at 4,215,840 to 4,216,919
_3620
Position (kb)
4214
4215
4216 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4213.804 kb on - strand at 4213.824 kb on - strand at 4214.271 kb on - strand at 4214.578 kb on + strand, within Pf6N2E2_3619 at 4214.579 kb on - strand, within Pf6N2E2_3619 at 4214.743 kb on - strand, within Pf6N2E2_3619 at 4214.743 kb on - strand, within Pf6N2E2_3619 at 4214.743 kb on - strand, within Pf6N2E2_3619 at 4214.743 kb on - strand, within Pf6N2E2_3619 at 4214.743 kb on - strand, within Pf6N2E2_3619 at 4214.916 kb on + strand, within Pf6N2E2_3619 at 4214.916 kb on + strand, within Pf6N2E2_3619 at 4215.087 kb on - strand, within Pf6N2E2_3619 at 4215.132 kb on - strand, within Pf6N2E2_3619 at 4215.132 kb on - strand, within Pf6N2E2_3619 at 4215.132 kb on - strand, within Pf6N2E2_3619 at 4215.132 kb on - strand, within Pf6N2E2_3619 at 4215.132 kb on - strand, within Pf6N2E2_3619 at 4215.132 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.350 kb on - strand, within Pf6N2E2_3619 at 4215.740 kb on - strand at 4215.740 kb on - strand at 4215.740 kb on - strand at 4215.740 kb on - strand at 4215.765 kb on - strand at 4215.765 kb on - strand at 4215.765 kb on - strand at 4215.808 kb on - strand at 4215.808 kb on - strand at 4215.808 kb on - strand at 4215.809 kb on + strand at 4215.809 kb on + strand at 4215.809 kb on + strand at 4215.810 kb on - strand at 4215.810 kb on - strand at 4215.810 kb on - strand at 4215.810 kb on - strand at 4215.810 kb on - strand at 4216.002 kb on + strand, within Pf6N2E2_3620 at 4216.002 kb on + strand, within Pf6N2E2_3620 at 4216.002 kb on + strand, within Pf6N2E2_3620 at 4216.003 kb on - strand, within Pf6N2E2_3620 at 4216.003 kb on - strand, within Pf6N2E2_3620 at 4216.003 kb on - strand, within Pf6N2E2_3620 at 4216.003 kb on - strand, within Pf6N2E2_3620 at 4216.087 kb on - strand, within Pf6N2E2_3620 at 4216.087 kb on - strand, within Pf6N2E2_3620 at 4216.087 kb on - strand, within Pf6N2E2_3620 at 4216.140 kb on + strand, within Pf6N2E2_3620 at 4216.140 kb on + strand, within Pf6N2E2_3620 at 4216.140 kb on + strand, within Pf6N2E2_3620 at 4216.141 kb on - strand, within Pf6N2E2_3620 at 4216.141 kb on - strand, within Pf6N2E2_3620 at 4216.141 kb on - strand, within Pf6N2E2_3620 at 4216.152 kb on + strand, within Pf6N2E2_3620 at 4216.170 kb on + strand, within Pf6N2E2_3620 at 4216.317 kb on - strand, within Pf6N2E2_3620 at 4216.353 kb on + strand, within Pf6N2E2_3620 at 4216.353 kb on + strand, within Pf6N2E2_3620 at 4216.353 kb on + strand, within Pf6N2E2_3620 at 4216.354 kb on - strand, within Pf6N2E2_3620 at 4216.457 kb on + strand, within Pf6N2E2_3620 at 4216.457 kb on + strand, within Pf6N2E2_3620 at 4216.457 kb on + strand, within Pf6N2E2_3620 at 4216.458 kb on - strand, within Pf6N2E2_3620 at 4216.479 kb on + strand, within Pf6N2E2_3620 at 4216.479 kb on + strand, within Pf6N2E2_3620 at 4216.480 kb on - strand, within Pf6N2E2_3620
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 4,213,804 - -1.0 4,213,824 - -2.8 4,214,271 - -2.0 4,214,578 + Pf6N2E2_3619 0.20 +0.1 4,214,579 - Pf6N2E2_3619 0.20 +2.1 4,214,743 - Pf6N2E2_3619 0.31 +1.4 4,214,743 - Pf6N2E2_3619 0.31 +0.9 4,214,743 - Pf6N2E2_3619 0.31 +1.5 4,214,743 - Pf6N2E2_3619 0.31 +1.7 4,214,743 - Pf6N2E2_3619 0.31 -0.6 4,214,916 + Pf6N2E2_3619 0.43 +0.9 4,214,916 + Pf6N2E2_3619 0.43 -1.7 4,215,087 - Pf6N2E2_3619 0.54 +1.8 4,215,132 - Pf6N2E2_3619 0.57 +0.9 4,215,132 - Pf6N2E2_3619 0.57 +1.5 4,215,132 - Pf6N2E2_3619 0.57 +0.1 4,215,132 - Pf6N2E2_3619 0.57 +2.2 4,215,132 - Pf6N2E2_3619 0.57 +1.8 4,215,132 - Pf6N2E2_3619 0.57 +1.6 4,215,350 - Pf6N2E2_3619 0.72 +1.5 4,215,350 - Pf6N2E2_3619 0.72 +0.7 4,215,350 - Pf6N2E2_3619 0.72 +1.4 4,215,350 - Pf6N2E2_3619 0.72 +1.2 4,215,350 - Pf6N2E2_3619 0.72 +1.0 4,215,350 - Pf6N2E2_3619 0.72 +1.9 4,215,350 - Pf6N2E2_3619 0.72 +1.4 4,215,350 - Pf6N2E2_3619 0.72 +1.6 4,215,740 - +1.2 4,215,740 - -0.7 4,215,740 - +0.1 4,215,740 - +0.7 4,215,765 - -0.2 4,215,765 - +0.8 4,215,765 - +0.2 4,215,808 - +1.8 4,215,808 - +2.4 4,215,808 - +2.6 4,215,809 + -0.2 4,215,809 + +0.1 4,215,809 + +0.8 4,215,810 - +1.9 4,215,810 - +1.6 4,215,810 - +2.2 4,215,810 - +2.9 4,215,810 - +2.0 4,216,002 + Pf6N2E2_3620 0.15 -0.7 4,216,002 + Pf6N2E2_3620 0.15 -1.5 4,216,002 + Pf6N2E2_3620 0.15 +0.5 4,216,003 - Pf6N2E2_3620 0.15 +2.1 4,216,003 - Pf6N2E2_3620 0.15 +1.3 4,216,003 - Pf6N2E2_3620 0.15 +1.4 4,216,003 - Pf6N2E2_3620 0.15 +1.2 4,216,087 - Pf6N2E2_3620 0.23 +1.5 4,216,087 - Pf6N2E2_3620 0.23 +1.6 4,216,087 - Pf6N2E2_3620 0.23 +1.7 4,216,140 + Pf6N2E2_3620 0.28 +0.5 4,216,140 + Pf6N2E2_3620 0.28 +0.3 4,216,140 + Pf6N2E2_3620 0.28 +0.0 4,216,141 - Pf6N2E2_3620 0.28 +2.9 4,216,141 - Pf6N2E2_3620 0.28 +2.0 4,216,141 - Pf6N2E2_3620 0.28 +0.6 4,216,152 + Pf6N2E2_3620 0.29 +0.7 4,216,170 + Pf6N2E2_3620 0.31 +0.3 4,216,317 - Pf6N2E2_3620 0.44 +2.0 4,216,353 + Pf6N2E2_3620 0.47 +0.2 4,216,353 + Pf6N2E2_3620 0.47 -0.5 4,216,353 + Pf6N2E2_3620 0.47 -0.7 4,216,354 - Pf6N2E2_3620 0.48 +0.0 4,216,457 + Pf6N2E2_3620 0.57 +0.4 4,216,457 + Pf6N2E2_3620 0.57 +0.5 4,216,457 + Pf6N2E2_3620 0.57 +0.5 4,216,458 - Pf6N2E2_3620 0.57 +1.6 4,216,479 + Pf6N2E2_3620 0.59 -0.1 4,216,479 + Pf6N2E2_3620 0.59 +1.4 4,216,480 - Pf6N2E2_3620 0.59 +2.2
Or see this region's nucleotide sequence