Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3527 and Pf6N2E2_3528 are separated by 131 nucleotides Pf6N2E2_3528 and Pf6N2E2_3529 are separated by 133 nucleotides Pf6N2E2_3529 and Pf6N2E2_3530 are separated by 10 nucleotides
Pf6N2E2_3527 - hypothetical protein, at 4,112,749 to 4,113,399
_3527
Pf6N2E2_3528 - hypothetical protein, at 4,113,531 to 4,114,073
_3528
Pf6N2E2_3529 - Toxin A, at 4,114,207 to 4,114,848
_3529
Pf6N2E2_3530 - Toxin TcdB, at 4,114,859 to 4,120,618
_3530
Position (kb)
4114
4115 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4113.225 kb on - strand, within Pf6N2E2_3527 at 4113.337 kb on - strand at 4113.376 kb on - strand at 4113.408 kb on + strand at 4113.408 kb on + strand at 4113.409 kb on - strand at 4113.409 kb on - strand at 4113.497 kb on + strand at 4113.501 kb on + strand at 4113.502 kb on - strand at 4113.502 kb on - strand at 4113.559 kb on + strand at 4113.559 kb on + strand at 4113.560 kb on - strand at 4113.646 kb on + strand, within Pf6N2E2_3528 at 4113.646 kb on + strand, within Pf6N2E2_3528 at 4113.646 kb on + strand, within Pf6N2E2_3528 at 4113.647 kb on - strand, within Pf6N2E2_3528 at 4113.647 kb on - strand, within Pf6N2E2_3528 at 4113.647 kb on - strand, within Pf6N2E2_3528 at 4113.706 kb on + strand, within Pf6N2E2_3528 at 4113.706 kb on + strand, within Pf6N2E2_3528 at 4113.706 kb on + strand, within Pf6N2E2_3528 at 4113.707 kb on - strand, within Pf6N2E2_3528 at 4113.707 kb on - strand, within Pf6N2E2_3528 at 4113.707 kb on - strand, within Pf6N2E2_3528 at 4113.784 kb on - strand, within Pf6N2E2_3528 at 4113.784 kb on - strand, within Pf6N2E2_3528 at 4113.784 kb on - strand, within Pf6N2E2_3528 at 4113.784 kb on - strand, within Pf6N2E2_3528 at 4114.033 kb on + strand at 4114.033 kb on + strand at 4114.033 kb on + strand at 4114.033 kb on + strand at 4114.033 kb on + strand at 4114.033 kb on + strand at 4114.033 kb on + strand at 4114.033 kb on + strand at 4114.033 kb on + strand at 4114.034 kb on - strand at 4114.034 kb on - strand at 4114.034 kb on - strand at 4114.034 kb on - strand at 4114.074 kb on + strand at 4114.075 kb on - strand at 4114.131 kb on + strand at 4114.132 kb on - strand at 4114.195 kb on + strand at 4114.195 kb on + strand at 4114.195 kb on + strand at 4114.195 kb on + strand at 4114.195 kb on + strand at 4114.196 kb on - strand at 4114.196 kb on - strand at 4114.196 kb on - strand at 4114.196 kb on - strand at 4114.196 kb on - strand at 4114.196 kb on - strand at 4114.196 kb on - strand at 4114.647 kb on - strand, within Pf6N2E2_3529 at 4114.647 kb on - strand, within Pf6N2E2_3529 at 4114.820 kb on + strand at 4114.821 kb on - strand at 4114.929 kb on - strand at 4114.974 kb on + strand at 4114.974 kb on + strand at 4114.974 kb on + strand at 4114.974 kb on + strand at 4114.974 kb on + strand at 4114.975 kb on - strand at 4114.975 kb on - strand at 4114.975 kb on - strand at 4114.975 kb on - strand at 4114.975 kb on - strand at 4114.975 kb on - strand at 4114.985 kb on + strand at 4114.985 kb on + strand at 4114.985 kb on + strand at 4114.985 kb on + strand at 4114.985 kb on + strand at 4114.986 kb on - strand at 4114.986 kb on - strand at 4114.986 kb on - strand at 4114.986 kb on - strand at 4114.986 kb on - strand at 4115.210 kb on - strand at 4115.240 kb on + strand at 4115.241 kb on - strand at 4115.349 kb on + strand at 4115.349 kb on + strand at 4115.350 kb on - strand at 4115.350 kb on - strand at 4115.459 kb on - strand, within Pf6N2E2_3530 at 4115.459 kb on - strand, within Pf6N2E2_3530 at 4115.536 kb on - strand, within Pf6N2E2_3530 at 4115.541 kb on + strand, within Pf6N2E2_3530 at 4115.607 kb on + strand, within Pf6N2E2_3530 at 4115.607 kb on + strand, within Pf6N2E2_3530 at 4115.608 kb on - strand, within Pf6N2E2_3530 at 4115.608 kb on - strand, within Pf6N2E2_3530 at 4115.634 kb on + strand, within Pf6N2E2_3530 at 4115.635 kb on - strand, within Pf6N2E2_3530 at 4115.635 kb on - strand, within Pf6N2E2_3530 at 4115.650 kb on - strand, within Pf6N2E2_3530 at 4115.703 kb on + strand, within Pf6N2E2_3530 at 4115.703 kb on + strand, within Pf6N2E2_3530 at 4115.703 kb on + strand, within Pf6N2E2_3530 at 4115.704 kb on - strand, within Pf6N2E2_3530 at 4115.704 kb on - strand, within Pf6N2E2_3530 at 4115.832 kb on - strand, within Pf6N2E2_3530 at 4115.832 kb on - strand, within Pf6N2E2_3530 at 4115.832 kb on - strand, within Pf6N2E2_3530 at 4115.832 kb on - strand, within Pf6N2E2_3530 at 4115.832 kb on - strand, within Pf6N2E2_3530
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 4,113,225 - Pf6N2E2_3527 0.73 +0.5 4,113,337 - +0.6 4,113,376 - -0.8 4,113,408 + -0.4 4,113,408 + +0.5 4,113,409 - +1.9 4,113,409 - +0.7 4,113,497 + -0.6 4,113,501 + -0.1 4,113,502 - +0.4 4,113,502 - +0.3 4,113,559 + +2.3 4,113,559 + -0.1 4,113,560 - -1.2 4,113,646 + Pf6N2E2_3528 0.21 -0.4 4,113,646 + Pf6N2E2_3528 0.21 +0.3 4,113,646 + Pf6N2E2_3528 0.21 +0.2 4,113,647 - Pf6N2E2_3528 0.21 +0.6 4,113,647 - Pf6N2E2_3528 0.21 -0.3 4,113,647 - Pf6N2E2_3528 0.21 -0.6 4,113,706 + Pf6N2E2_3528 0.32 +0.3 4,113,706 + Pf6N2E2_3528 0.32 -1.0 4,113,706 + Pf6N2E2_3528 0.32 +1.0 4,113,707 - Pf6N2E2_3528 0.32 -0.2 4,113,707 - Pf6N2E2_3528 0.32 -0.1 4,113,707 - Pf6N2E2_3528 0.32 +1.1 4,113,784 - Pf6N2E2_3528 0.47 -1.2 4,113,784 - Pf6N2E2_3528 0.47 +0.8 4,113,784 - Pf6N2E2_3528 0.47 +0.7 4,113,784 - Pf6N2E2_3528 0.47 -0.2 4,114,033 + -0.0 4,114,033 + +0.0 4,114,033 + +0.5 4,114,033 + +0.8 4,114,033 + +0.4 4,114,033 + +0.8 4,114,033 + -0.7 4,114,033 + -1.1 4,114,033 + +0.4 4,114,034 - -0.9 4,114,034 - +0.4 4,114,034 - +1.4 4,114,034 - +0.6 4,114,074 + +0.3 4,114,075 - -0.7 4,114,131 + -0.1 4,114,132 - +0.8 4,114,195 + +1.1 4,114,195 + -0.3 4,114,195 + -0.2 4,114,195 + +0.3 4,114,195 + -0.9 4,114,196 - +0.2 4,114,196 - +1.7 4,114,196 - +0.2 4,114,196 - -0.5 4,114,196 - -1.1 4,114,196 - +0.1 4,114,196 - -0.5 4,114,647 - Pf6N2E2_3529 0.69 -1.3 4,114,647 - Pf6N2E2_3529 0.69 -0.7 4,114,820 + +1.3 4,114,821 - +0.1 4,114,929 - -0.1 4,114,974 + -1.3 4,114,974 + -0.5 4,114,974 + +0.6 4,114,974 + -2.3 4,114,974 + -0.7 4,114,975 - -0.8 4,114,975 - +1.4 4,114,975 - +0.6 4,114,975 - -0.5 4,114,975 - -0.8 4,114,975 - +0.2 4,114,985 + -0.0 4,114,985 + +0.4 4,114,985 + -0.7 4,114,985 + -0.6 4,114,985 + -2.4 4,114,986 - +0.4 4,114,986 - -0.8 4,114,986 - +0.1 4,114,986 - +0.5 4,114,986 - -0.3 4,115,210 - -0.5 4,115,240 + +0.0 4,115,241 - -0.1 4,115,349 + -0.6 4,115,349 + +0.2 4,115,350 - -1.0 4,115,350 - +0.3 4,115,459 - Pf6N2E2_3530 0.10 -0.6 4,115,459 - Pf6N2E2_3530 0.10 +2.1 4,115,536 - Pf6N2E2_3530 0.12 -1.8 4,115,541 + Pf6N2E2_3530 0.12 +0.7 4,115,607 + Pf6N2E2_3530 0.13 -0.9 4,115,607 + Pf6N2E2_3530 0.13 -2.0 4,115,608 - Pf6N2E2_3530 0.13 -1.7 4,115,608 - Pf6N2E2_3530 0.13 +0.6 4,115,634 + Pf6N2E2_3530 0.13 +0.4 4,115,635 - Pf6N2E2_3530 0.13 -0.1 4,115,635 - Pf6N2E2_3530 0.13 +0.4 4,115,650 - Pf6N2E2_3530 0.14 +1.0 4,115,703 + Pf6N2E2_3530 0.15 +0.4 4,115,703 + Pf6N2E2_3530 0.15 -0.5 4,115,703 + Pf6N2E2_3530 0.15 +0.6 4,115,704 - Pf6N2E2_3530 0.15 +0.7 4,115,704 - Pf6N2E2_3530 0.15 -2.6 4,115,832 - Pf6N2E2_3530 0.17 +0.2 4,115,832 - Pf6N2E2_3530 0.17 -0.1 4,115,832 - Pf6N2E2_3530 0.17 -0.1 4,115,832 - Pf6N2E2_3530 0.17 +0.6 4,115,832 - Pf6N2E2_3530 0.17 +0.2
Or see this region's nucleotide sequence