Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3515 and Pf6N2E2_3516 are separated by 13 nucleotides Pf6N2E2_3516 and Pf6N2E2_3517 are separated by 147 nucleotides Pf6N2E2_3517 and Pf6N2E2_3518 are separated by 38 nucleotides Pf6N2E2_3518 and Pf6N2E2_3519 are separated by 83 nucleotides
Pf6N2E2_3515 - Probable transmembrane protein, at 4,099,860 to 4,100,942
_3515
Pf6N2E2_3516 - Probable transmembrane protein, at 4,100,956 to 4,101,090
_3516
Pf6N2E2_3517 - tRNA dihydrouridine synthase B (EC 1.-.-.-), at 4,101,238 to 4,102,245
_3517
Pf6N2E2_3518 - DNA-binding protein Fis, at 4,102,284 to 4,102,562
_3518
Pf6N2E2_3519 - IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3), at 4,102,646 to 4,104,253
_3519
Position (kb)
4101
4102
4103 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4100.256 kb on + strand, within Pf6N2E2_3515 at 4100.256 kb on + strand, within Pf6N2E2_3515 at 4100.603 kb on - strand, within Pf6N2E2_3515 at 4100.603 kb on - strand, within Pf6N2E2_3515 at 4100.635 kb on + strand, within Pf6N2E2_3515 at 4100.635 kb on + strand, within Pf6N2E2_3515 at 4100.635 kb on + strand, within Pf6N2E2_3515 at 4100.635 kb on + strand, within Pf6N2E2_3515 at 4100.636 kb on - strand, within Pf6N2E2_3515 at 4100.636 kb on - strand, within Pf6N2E2_3515 at 4100.636 kb on - strand, within Pf6N2E2_3515 at 4100.636 kb on - strand, within Pf6N2E2_3515 at 4100.723 kb on + strand, within Pf6N2E2_3515 at 4100.723 kb on + strand, within Pf6N2E2_3515 at 4100.821 kb on + strand, within Pf6N2E2_3515 at 4100.821 kb on + strand, within Pf6N2E2_3515 at 4100.821 kb on + strand, within Pf6N2E2_3515 at 4100.821 kb on + strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.923 kb on + strand at 4101.022 kb on + strand, within Pf6N2E2_3516 at 4101.022 kb on + strand, within Pf6N2E2_3516 at 4101.023 kb on - strand, within Pf6N2E2_3516 at 4101.129 kb on + strand at 4101.129 kb on + strand at 4101.130 kb on - strand at 4101.237 kb on + strand at 4101.237 kb on + strand at 4101.237 kb on + strand at 4101.237 kb on + strand at 4101.237 kb on + strand at 4101.262 kb on + strand at 4101.262 kb on + strand at 4101.262 kb on + strand at 4101.262 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.775 kb on + strand, within Pf6N2E2_3517 at 4101.775 kb on + strand, within Pf6N2E2_3517 at 4102.017 kb on + strand, within Pf6N2E2_3517 at 4102.017 kb on + strand, within Pf6N2E2_3517 at 4102.017 kb on + strand, within Pf6N2E2_3517 at 4102.017 kb on + strand, within Pf6N2E2_3517 at 4102.017 kb on + strand, within Pf6N2E2_3517 at 4102.017 kb on + strand, within Pf6N2E2_3517 at 4102.043 kb on + strand, within Pf6N2E2_3517 at 4102.043 kb on + strand, within Pf6N2E2_3517 at 4102.043 kb on + strand, within Pf6N2E2_3517 at 4102.043 kb on + strand, within Pf6N2E2_3517 at 4102.073 kb on + strand, within Pf6N2E2_3517 at 4102.161 kb on + strand at 4102.207 kb on + strand at 4102.207 kb on + strand at 4102.207 kb on + strand at 4102.264 kb on + strand at 4102.560 kb on + strand at 4102.560 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 4,100,256 + Pf6N2E2_3515 0.37 -2.4 4,100,256 + Pf6N2E2_3515 0.37 -3.3 4,100,603 - Pf6N2E2_3515 0.69 -0.8 4,100,603 - Pf6N2E2_3515 0.69 -1.0 4,100,635 + Pf6N2E2_3515 0.72 +0.0 4,100,635 + Pf6N2E2_3515 0.72 -1.4 4,100,635 + Pf6N2E2_3515 0.72 +1.0 4,100,635 + Pf6N2E2_3515 0.72 +0.4 4,100,636 - Pf6N2E2_3515 0.72 -0.9 4,100,636 - Pf6N2E2_3515 0.72 -0.7 4,100,636 - Pf6N2E2_3515 0.72 -0.4 4,100,636 - Pf6N2E2_3515 0.72 -0.5 4,100,723 + Pf6N2E2_3515 0.80 -0.2 4,100,723 + Pf6N2E2_3515 0.80 -0.0 4,100,821 + Pf6N2E2_3515 0.89 -2.1 4,100,821 + Pf6N2E2_3515 0.89 -0.8 4,100,821 + Pf6N2E2_3515 0.89 +0.7 4,100,821 + Pf6N2E2_3515 0.89 -0.6 4,100,822 - Pf6N2E2_3515 0.89 -0.0 4,100,822 - Pf6N2E2_3515 0.89 -1.6 4,100,822 - Pf6N2E2_3515 0.89 +0.0 4,100,822 - Pf6N2E2_3515 0.89 +0.1 4,100,822 - Pf6N2E2_3515 0.89 -1.0 4,100,923 + -0.8 4,101,022 + Pf6N2E2_3516 0.49 -1.2 4,101,022 + Pf6N2E2_3516 0.49 -2.6 4,101,023 - Pf6N2E2_3516 0.50 +0.2 4,101,129 + -3.5 4,101,129 + -1.8 4,101,130 - +2.0 4,101,237 + +0.8 4,101,237 + +0.8 4,101,237 + +0.1 4,101,237 + -0.1 4,101,237 + -3.2 4,101,262 + +1.2 4,101,262 + +0.5 4,101,262 + +0.2 4,101,262 + +0.3 4,101,264 + +1.2 4,101,264 + +0.5 4,101,264 + -0.1 4,101,264 + -0.0 4,101,264 + -0.0 4,101,264 + +0.4 4,101,264 + -1.3 4,101,264 + -0.3 4,101,264 + +1.2 4,101,264 + +1.3 4,101,264 + -1.0 4,101,264 + +0.4 4,101,264 + +0.5 4,101,264 + -1.7 4,101,264 + -0.2 4,101,264 + +0.0 4,101,264 + +0.9 4,101,264 + +0.7 4,101,775 + Pf6N2E2_3517 0.53 -0.8 4,101,775 + Pf6N2E2_3517 0.53 -0.8 4,102,017 + Pf6N2E2_3517 0.77 -2.6 4,102,017 + Pf6N2E2_3517 0.77 -2.7 4,102,017 + Pf6N2E2_3517 0.77 -1.5 4,102,017 + Pf6N2E2_3517 0.77 -3.7 4,102,017 + Pf6N2E2_3517 0.77 -3.3 4,102,017 + Pf6N2E2_3517 0.77 -2.3 4,102,043 + Pf6N2E2_3517 0.80 -4.1 4,102,043 + Pf6N2E2_3517 0.80 -3.6 4,102,043 + Pf6N2E2_3517 0.80 -1.0 4,102,043 + Pf6N2E2_3517 0.80 -2.7 4,102,073 + Pf6N2E2_3517 0.83 -2.2 4,102,161 + -0.5 4,102,207 + -2.1 4,102,207 + -2.6 4,102,207 + -1.3 4,102,264 + +0.9 4,102,560 + +1.4 4,102,560 + +0.2
Or see this region's nucleotide sequence