Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3283 and Pf6N2E2_3284 are separated by 56 nucleotides Pf6N2E2_3284 and Pf6N2E2_3285 are separated by 183 nucleotides Pf6N2E2_3285 and Pf6N2E2_3286 overlap by 4 nucleotides
Pf6N2E2_3283 - Cytoplasmic axial filament protein CafA and Ribonuclease G (EC 3.1.4.-), at 3,789,546 to 3,791,003
_3283
Pf6N2E2_3284 - Septum formation protein Maf, at 3,791,060 to 3,791,656
_3284
Pf6N2E2_3285 - Rod shape-determining protein MreD, at 3,791,840 to 3,792,328
_3285
Pf6N2E2_3286 - Rod shape-determining protein MreC, at 3,792,325 to 3,793,446
_3286
Position (kb)
3791
3792
3793 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3790.878 kb on - strand at 3790.880 kb on - strand at 3790.880 kb on - strand at 3790.880 kb on - strand at 3790.880 kb on - strand at 3790.884 kb on - strand at 3790.884 kb on - strand at 3790.884 kb on - strand at 3791.091 kb on - strand at 3791.091 kb on - strand at 3791.109 kb on - strand at 3791.109 kb on - strand at 3791.109 kb on - strand at 3791.109 kb on - strand at 3791.152 kb on - strand, within Pf6N2E2_3284 at 3791.152 kb on - strand, within Pf6N2E2_3284 at 3791.152 kb on - strand, within Pf6N2E2_3284 at 3791.198 kb on - strand, within Pf6N2E2_3284 at 3791.198 kb on - strand, within Pf6N2E2_3284 at 3791.198 kb on - strand, within Pf6N2E2_3284 at 3791.342 kb on + strand, within Pf6N2E2_3284 at 3791.343 kb on - strand, within Pf6N2E2_3284 at 3791.343 kb on - strand, within Pf6N2E2_3284 at 3791.343 kb on - strand, within Pf6N2E2_3284 at 3791.343 kb on - strand, within Pf6N2E2_3284 at 3791.343 kb on - strand, within Pf6N2E2_3284 at 3791.527 kb on - strand, within Pf6N2E2_3284 at 3791.644 kb on - strand at 3791.644 kb on - strand at 3791.644 kb on - strand at 3791.755 kb on - strand at 3791.804 kb on + strand at 3791.804 kb on + strand at 3791.805 kb on - strand at 3791.805 kb on - strand at 3791.805 kb on - strand at 3791.805 kb on - strand at 3791.805 kb on - strand at 3791.805 kb on - strand at 3791.805 kb on - strand at 3791.805 kb on - strand at 3791.805 kb on - strand at 3791.805 kb on - strand at 3791.805 kb on - strand at 3791.805 kb on - strand at 3791.805 kb on - strand at 3791.805 kb on - strand at 3791.805 kb on - strand at 3791.805 kb on - strand at 3791.805 kb on - strand at 3791.805 kb on - strand at 3791.805 kb on - strand at 3791.823 kb on + strand at 3791.824 kb on - strand at 3791.824 kb on - strand at 3791.828 kb on - strand at 3792.032 kb on - strand, within Pf6N2E2_3285 at 3792.032 kb on - strand, within Pf6N2E2_3285 at 3792.032 kb on - strand, within Pf6N2E2_3285 at 3792.032 kb on - strand, within Pf6N2E2_3285 at 3792.032 kb on - strand, within Pf6N2E2_3285 at 3792.032 kb on - strand, within Pf6N2E2_3285 at 3792.671 kb on - strand, within Pf6N2E2_3286
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 3,790,878 - -0.8 3,790,880 - +0.2 3,790,880 - +1.6 3,790,880 - +1.0 3,790,880 - +0.5 3,790,884 - +0.9 3,790,884 - +1.2 3,790,884 - -0.3 3,791,091 - -0.1 3,791,091 - +0.1 3,791,109 - -0.7 3,791,109 - +0.3 3,791,109 - -0.1 3,791,109 - +0.0 3,791,152 - Pf6N2E2_3284 0.15 -0.4 3,791,152 - Pf6N2E2_3284 0.15 +0.3 3,791,152 - Pf6N2E2_3284 0.15 -0.2 3,791,198 - Pf6N2E2_3284 0.23 -0.8 3,791,198 - Pf6N2E2_3284 0.23 -2.3 3,791,198 - Pf6N2E2_3284 0.23 -1.0 3,791,342 + Pf6N2E2_3284 0.47 +1.9 3,791,343 - Pf6N2E2_3284 0.47 -0.5 3,791,343 - Pf6N2E2_3284 0.47 -0.2 3,791,343 - Pf6N2E2_3284 0.47 +0.4 3,791,343 - Pf6N2E2_3284 0.47 -0.7 3,791,343 - Pf6N2E2_3284 0.47 -0.4 3,791,527 - Pf6N2E2_3284 0.78 -0.4 3,791,644 - -0.3 3,791,644 - +1.0 3,791,644 - -0.9 3,791,755 - -0.5 3,791,804 + +1.0 3,791,804 + +0.3 3,791,805 - -0.2 3,791,805 - -0.5 3,791,805 - -1.5 3,791,805 - -1.2 3,791,805 - -0.7 3,791,805 - -1.3 3,791,805 - -0.3 3,791,805 - -0.6 3,791,805 - +0.2 3,791,805 - -0.8 3,791,805 - -0.8 3,791,805 - -2.2 3,791,805 - -0.6 3,791,805 - -1.7 3,791,805 - -0.5 3,791,805 - -0.2 3,791,805 - -0.2 3,791,805 - -0.2 3,791,805 - -0.2 3,791,823 + +1.0 3,791,824 - -0.1 3,791,824 - -0.9 3,791,828 - -1.0 3,792,032 - Pf6N2E2_3285 0.39 -3.1 3,792,032 - Pf6N2E2_3285 0.39 -1.3 3,792,032 - Pf6N2E2_3285 0.39 -3.9 3,792,032 - Pf6N2E2_3285 0.39 -2.3 3,792,032 - Pf6N2E2_3285 0.39 -2.8 3,792,032 - Pf6N2E2_3285 0.39 -4.5 3,792,671 - Pf6N2E2_3286 0.31 -1.5
Or see this region's nucleotide sequence