Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3245 and Pf6N2E2_3246 are separated by 299 nucleotides Pf6N2E2_3246 and Pf6N2E2_3247 are separated by 45 nucleotides
Pf6N2E2_3245 - L-lysine permease, at 3,754,134 to 3,754,763
_3245
Pf6N2E2_3246 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 3,755,063 to 3,756,028
_3246
Pf6N2E2_3247 - Ribosomal RNA small subunit methyltransferase C (EC 2.1.1.52), at 3,756,074 to 3,757,072
_3247
Position (kb)
3755
3756
3757 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3754.297 kb on + strand, within Pf6N2E2_3245 at 3754.297 kb on + strand, within Pf6N2E2_3245 at 3754.298 kb on - strand, within Pf6N2E2_3245 at 3754.298 kb on - strand, within Pf6N2E2_3245 at 3754.434 kb on - strand, within Pf6N2E2_3245 at 3754.434 kb on - strand, within Pf6N2E2_3245 at 3754.510 kb on + strand, within Pf6N2E2_3245 at 3754.510 kb on + strand, within Pf6N2E2_3245 at 3754.510 kb on + strand, within Pf6N2E2_3245 at 3754.510 kb on + strand, within Pf6N2E2_3245 at 3754.510 kb on + strand, within Pf6N2E2_3245 at 3754.510 kb on + strand, within Pf6N2E2_3245 at 3754.511 kb on - strand, within Pf6N2E2_3245 at 3754.511 kb on - strand, within Pf6N2E2_3245 at 3754.511 kb on - strand, within Pf6N2E2_3245 at 3754.511 kb on - strand, within Pf6N2E2_3245 at 3754.511 kb on - strand, within Pf6N2E2_3245 at 3754.511 kb on - strand, within Pf6N2E2_3245 at 3754.519 kb on + strand, within Pf6N2E2_3245 at 3754.520 kb on - strand, within Pf6N2E2_3245 at 3754.759 kb on + strand at 3754.759 kb on + strand at 3754.760 kb on - strand at 3754.760 kb on - strand at 3754.823 kb on - strand at 3754.823 kb on - strand at 3754.869 kb on + strand at 3754.869 kb on + strand at 3754.870 kb on - strand at 3754.870 kb on - strand at 3754.870 kb on - strand at 3754.870 kb on - strand at 3754.870 kb on - strand at 3754.870 kb on - strand at 3754.877 kb on + strand at 3754.878 kb on - strand at 3754.942 kb on + strand at 3755.000 kb on + strand at 3755.000 kb on + strand at 3755.000 kb on + strand at 3755.000 kb on + strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.001 kb on - strand at 3755.002 kb on + strand at 3755.028 kb on + strand at 3755.028 kb on + strand at 3755.028 kb on + strand at 3755.029 kb on - strand at 3755.055 kb on + strand at 3755.056 kb on - strand at 3755.056 kb on - strand at 3755.056 kb on - strand at 3755.056 kb on - strand at 3756.005 kb on - strand at 3756.029 kb on + strand at 3756.030 kb on - strand at 3756.058 kb on + strand at 3756.059 kb on - strand at 3756.059 kb on - strand at 3756.059 kb on - strand at 3756.059 kb on - strand at 3756.094 kb on - strand at 3756.094 kb on - strand at 3756.094 kb on - strand at 3756.115 kb on - strand at 3756.323 kb on + strand, within Pf6N2E2_3247 at 3756.323 kb on + strand, within Pf6N2E2_3247 at 3756.324 kb on - strand, within Pf6N2E2_3247 at 3756.324 kb on - strand, within Pf6N2E2_3247 at 3756.324 kb on - strand, within Pf6N2E2_3247 at 3756.368 kb on + strand, within Pf6N2E2_3247 at 3756.369 kb on - strand, within Pf6N2E2_3247 at 3756.369 kb on - strand, within Pf6N2E2_3247 at 3756.369 kb on - strand, within Pf6N2E2_3247 at 3756.369 kb on - strand, within Pf6N2E2_3247 at 3756.369 kb on - strand, within Pf6N2E2_3247 at 3756.369 kb on - strand, within Pf6N2E2_3247 at 3756.531 kb on - strand, within Pf6N2E2_3247 at 3756.894 kb on - strand, within Pf6N2E2_3247
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 3,754,297 + Pf6N2E2_3245 0.26 +0.7 3,754,297 + Pf6N2E2_3245 0.26 +0.2 3,754,298 - Pf6N2E2_3245 0.26 +0.7 3,754,298 - Pf6N2E2_3245 0.26 -0.6 3,754,434 - Pf6N2E2_3245 0.48 -1.0 3,754,434 - Pf6N2E2_3245 0.48 +0.0 3,754,510 + Pf6N2E2_3245 0.60 +0.4 3,754,510 + Pf6N2E2_3245 0.60 -1.0 3,754,510 + Pf6N2E2_3245 0.60 +0.2 3,754,510 + Pf6N2E2_3245 0.60 -0.3 3,754,510 + Pf6N2E2_3245 0.60 -1.2 3,754,510 + Pf6N2E2_3245 0.60 +0.8 3,754,511 - Pf6N2E2_3245 0.60 +0.6 3,754,511 - Pf6N2E2_3245 0.60 -1.1 3,754,511 - Pf6N2E2_3245 0.60 +0.3 3,754,511 - Pf6N2E2_3245 0.60 +0.1 3,754,511 - Pf6N2E2_3245 0.60 -0.2 3,754,511 - Pf6N2E2_3245 0.60 -0.7 3,754,519 + Pf6N2E2_3245 0.61 +0.2 3,754,520 - Pf6N2E2_3245 0.61 -1.1 3,754,759 + +0.7 3,754,759 + -0.3 3,754,760 - -0.2 3,754,760 - +1.2 3,754,823 - +0.0 3,754,823 - -0.9 3,754,869 + +0.7 3,754,869 + +0.1 3,754,870 - +0.5 3,754,870 - +0.6 3,754,870 - -0.2 3,754,870 - +0.7 3,754,870 - +1.6 3,754,870 - -2.7 3,754,877 + +0.2 3,754,878 - +0.1 3,754,942 + +0.7 3,755,000 + +0.2 3,755,000 + -0.1 3,755,000 + +0.5 3,755,000 + -0.7 3,755,001 - -1.6 3,755,001 - +1.1 3,755,001 - +2.9 3,755,001 - -0.7 3,755,001 - +0.1 3,755,001 - +0.1 3,755,001 - -0.1 3,755,001 - +0.6 3,755,001 - -0.9 3,755,001 - +1.0 3,755,001 - +0.7 3,755,002 + +0.7 3,755,028 + +0.2 3,755,028 + -0.1 3,755,028 + +0.5 3,755,029 - -0.1 3,755,055 + +0.8 3,755,056 - +0.5 3,755,056 - +1.1 3,755,056 - -0.7 3,755,056 - -0.6 3,756,005 - +0.2 3,756,029 + -0.9 3,756,030 - -1.0 3,756,058 + -0.9 3,756,059 - -0.6 3,756,059 - -0.9 3,756,059 - +0.0 3,756,059 - +0.0 3,756,094 - +1.3 3,756,094 - -0.4 3,756,094 - -0.2 3,756,115 - +0.7 3,756,323 + Pf6N2E2_3247 0.25 +0.9 3,756,323 + Pf6N2E2_3247 0.25 -0.7 3,756,324 - Pf6N2E2_3247 0.25 -0.9 3,756,324 - Pf6N2E2_3247 0.25 -1.6 3,756,324 - Pf6N2E2_3247 0.25 -0.3 3,756,368 + Pf6N2E2_3247 0.29 +0.1 3,756,369 - Pf6N2E2_3247 0.30 -0.7 3,756,369 - Pf6N2E2_3247 0.30 -0.6 3,756,369 - Pf6N2E2_3247 0.30 -1.2 3,756,369 - Pf6N2E2_3247 0.30 +0.2 3,756,369 - Pf6N2E2_3247 0.30 +0.2 3,756,369 - Pf6N2E2_3247 0.30 +0.4 3,756,531 - Pf6N2E2_3247 0.46 -1.2 3,756,894 - Pf6N2E2_3247 0.82 -1.0
Or see this region's nucleotide sequence