Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_299 and Pf6N2E2_300 overlap by 4 nucleotides Pf6N2E2_300 and Pf6N2E2_301 are separated by 12 nucleotides Pf6N2E2_301 and Pf6N2E2_302 are separated by 0 nucleotides
Pf6N2E2_299 - FIG065221: Holliday junction DNA helicase, at 369,402 to 370,727
_299
Pf6N2E2_300 - CrcB protein, at 370,724 to 371,101
_300
Pf6N2E2_301 - Seryl-tRNA synthetase (EC 6.1.1.11), at 371,114 to 372,394
_301
Pf6N2E2_302 - Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 372,395 to 373,789
_302
Position (kb) 371
372
373 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 370.207 kb on - strand, within Pf6N2E2_299 at 370.248 kb on + strand, within Pf6N2E2_299 at 370.248 kb on + strand, within Pf6N2E2_299 at 370.249 kb on - strand, within Pf6N2E2_299 at 370.395 kb on + strand, within Pf6N2E2_299 at 370.395 kb on + strand, within Pf6N2E2_299 at 370.395 kb on + strand, within Pf6N2E2_299 at 370.396 kb on - strand, within Pf6N2E2_299 at 370.396 kb on - strand, within Pf6N2E2_299 at 370.428 kb on + strand, within Pf6N2E2_299 at 370.429 kb on - strand, within Pf6N2E2_299 at 370.429 kb on - strand, within Pf6N2E2_299 at 370.552 kb on - strand, within Pf6N2E2_299 at 370.575 kb on + strand, within Pf6N2E2_299 at 370.575 kb on + strand, within Pf6N2E2_299 at 370.575 kb on + strand, within Pf6N2E2_299 at 370.593 kb on + strand, within Pf6N2E2_299 at 370.594 kb on - strand, within Pf6N2E2_299 at 370.626 kb on + strand at 370.627 kb on - strand at 370.826 kb on + strand, within Pf6N2E2_300 at 370.826 kb on + strand, within Pf6N2E2_300 at 370.826 kb on + strand, within Pf6N2E2_300 at 370.826 kb on + strand, within Pf6N2E2_300 at 370.846 kb on + strand, within Pf6N2E2_300 at 370.849 kb on + strand, within Pf6N2E2_300 at 370.849 kb on + strand, within Pf6N2E2_300 at 370.849 kb on + strand, within Pf6N2E2_300 at 371.099 kb on + strand at 372.401 kb on + strand at 372.587 kb on + strand, within Pf6N2E2_302 at 372.588 kb on - strand, within Pf6N2E2_302 at 373.049 kb on + strand, within Pf6N2E2_302 at 373.193 kb on + strand, within Pf6N2E2_302 at 373.193 kb on + strand, within Pf6N2E2_302
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 370,207 - Pf6N2E2_299 0.61 -0.0 370,248 + Pf6N2E2_299 0.64 +2.1 370,248 + Pf6N2E2_299 0.64 -1.0 370,249 - Pf6N2E2_299 0.64 +1.3 370,395 + Pf6N2E2_299 0.75 +0.5 370,395 + Pf6N2E2_299 0.75 +0.5 370,395 + Pf6N2E2_299 0.75 -0.8 370,396 - Pf6N2E2_299 0.75 +0.1 370,396 - Pf6N2E2_299 0.75 +0.7 370,428 + Pf6N2E2_299 0.77 -0.5 370,429 - Pf6N2E2_299 0.77 +0.7 370,429 - Pf6N2E2_299 0.77 -3.6 370,552 - Pf6N2E2_299 0.87 +0.1 370,575 + Pf6N2E2_299 0.88 +0.3 370,575 + Pf6N2E2_299 0.88 -0.1 370,575 + Pf6N2E2_299 0.88 -2.7 370,593 + Pf6N2E2_299 0.90 -0.9 370,594 - Pf6N2E2_299 0.90 -0.2 370,626 + +0.9 370,627 - -1.1 370,826 + Pf6N2E2_300 0.27 +0.8 370,826 + Pf6N2E2_300 0.27 +1.8 370,826 + Pf6N2E2_300 0.27 -0.3 370,826 + Pf6N2E2_300 0.27 +0.8 370,846 + Pf6N2E2_300 0.32 -0.1 370,849 + Pf6N2E2_300 0.33 +1.5 370,849 + Pf6N2E2_300 0.33 +0.7 370,849 + Pf6N2E2_300 0.33 +0.2 371,099 + -0.0 372,401 + -3.5 372,587 + Pf6N2E2_302 0.14 -1.3 372,588 - Pf6N2E2_302 0.14 +0.2 373,049 + Pf6N2E2_302 0.47 -1.6 373,193 + Pf6N2E2_302 0.57 +0.8 373,193 + Pf6N2E2_302 0.57 -0.0
Or see this region's nucleotide sequence