Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2939 and Pf6N2E2_2940 are separated by 24 nucleotides Pf6N2E2_2940 and Pf6N2E2_2941 are separated by 116 nucleotides
Pf6N2E2_2939 - Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5), at 3,430,977 to 3,431,624
_2939
Pf6N2E2_2940 - OmpA family protein, at 3,431,649 to 3,432,761
_2940
Pf6N2E2_2941 - Esterase A, at 3,432,878 to 3,434,023
_2941
Position (kb)
3431
3432
3433 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3430.700 kb on + strand at 3430.752 kb on - strand at 3430.752 kb on - strand at 3430.787 kb on + strand at 3430.787 kb on + strand at 3430.788 kb on - strand at 3430.788 kb on - strand at 3430.788 kb on - strand at 3430.805 kb on + strand at 3430.805 kb on + strand at 3430.805 kb on + strand at 3430.805 kb on + strand at 3430.806 kb on - strand at 3430.880 kb on + strand at 3430.880 kb on + strand at 3430.880 kb on + strand at 3430.880 kb on + strand at 3430.880 kb on + strand at 3430.880 kb on + strand at 3430.880 kb on + strand at 3430.880 kb on + strand at 3430.880 kb on + strand at 3430.881 kb on - strand at 3430.881 kb on - strand at 3430.881 kb on - strand at 3430.881 kb on - strand at 3430.978 kb on + strand at 3431.902 kb on + strand, within Pf6N2E2_2940 at 3431.902 kb on + strand, within Pf6N2E2_2940 at 3431.902 kb on + strand, within Pf6N2E2_2940 at 3431.902 kb on + strand, within Pf6N2E2_2940 at 3431.902 kb on + strand, within Pf6N2E2_2940 at 3431.902 kb on + strand, within Pf6N2E2_2940 at 3431.903 kb on - strand, within Pf6N2E2_2940 at 3431.903 kb on - strand, within Pf6N2E2_2940 at 3431.903 kb on - strand, within Pf6N2E2_2940 at 3431.903 kb on - strand, within Pf6N2E2_2940 at 3432.341 kb on - strand, within Pf6N2E2_2940 at 3432.369 kb on - strand, within Pf6N2E2_2940 at 3432.750 kb on - strand at 3432.782 kb on + strand at 3432.782 kb on + strand at 3432.782 kb on + strand at 3432.783 kb on - strand at 3432.783 kb on - strand at 3432.783 kb on - strand at 3432.783 kb on - strand at 3432.794 kb on - strand at 3432.794 kb on - strand at 3432.879 kb on + strand at 3432.880 kb on - strand at 3432.881 kb on + strand at 3432.881 kb on + strand at 3432.881 kb on + strand at 3432.881 kb on + strand at 3432.882 kb on - strand at 3432.882 kb on - strand at 3432.882 kb on - strand at 3432.932 kb on + strand at 3432.932 kb on + strand at 3432.932 kb on + strand at 3432.932 kb on + strand at 3432.932 kb on + strand at 3432.932 kb on + strand at 3432.933 kb on - strand at 3432.933 kb on - strand at 3432.933 kb on - strand at 3432.933 kb on - strand at 3432.933 kb on - strand at 3432.933 kb on - strand at 3432.933 kb on - strand at 3432.933 kb on - strand at 3432.933 kb on - strand at 3432.936 kb on + strand at 3432.936 kb on + strand at 3432.937 kb on - strand at 3432.937 kb on - strand at 3432.937 kb on - strand at 3432.937 kb on - strand at 3433.048 kb on - strand, within Pf6N2E2_2941 at 3433.048 kb on - strand, within Pf6N2E2_2941 at 3433.062 kb on - strand, within Pf6N2E2_2941 at 3433.200 kb on + strand, within Pf6N2E2_2941 at 3433.542 kb on + strand, within Pf6N2E2_2941 at 3433.543 kb on - strand, within Pf6N2E2_2941 at 3433.557 kb on + strand, within Pf6N2E2_2941 at 3433.558 kb on - strand, within Pf6N2E2_2941 at 3433.629 kb on + strand, within Pf6N2E2_2941 at 3433.629 kb on + strand, within Pf6N2E2_2941 at 3433.629 kb on + strand, within Pf6N2E2_2941 at 3433.629 kb on + strand, within Pf6N2E2_2941
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 3,430,700 + -2.1 3,430,752 - -0.4 3,430,752 - -0.4 3,430,787 + +0.3 3,430,787 + +0.7 3,430,788 - +0.3 3,430,788 - +0.9 3,430,788 - -2.7 3,430,805 + -1.0 3,430,805 + +0.5 3,430,805 + -0.5 3,430,805 + -0.2 3,430,806 - +1.2 3,430,880 + -0.1 3,430,880 + +0.0 3,430,880 + -1.1 3,430,880 + -2.7 3,430,880 + +0.2 3,430,880 + +0.9 3,430,880 + -0.4 3,430,880 + -1.0 3,430,880 + +0.7 3,430,881 - -1.0 3,430,881 - -0.2 3,430,881 - -0.8 3,430,881 - -0.2 3,430,978 + -0.1 3,431,902 + Pf6N2E2_2940 0.23 -2.1 3,431,902 + Pf6N2E2_2940 0.23 -2.4 3,431,902 + Pf6N2E2_2940 0.23 -1.2 3,431,902 + Pf6N2E2_2940 0.23 -1.9 3,431,902 + Pf6N2E2_2940 0.23 -3.3 3,431,902 + Pf6N2E2_2940 0.23 -2.2 3,431,903 - Pf6N2E2_2940 0.23 -1.3 3,431,903 - Pf6N2E2_2940 0.23 -0.4 3,431,903 - Pf6N2E2_2940 0.23 -2.3 3,431,903 - Pf6N2E2_2940 0.23 -2.7 3,432,341 - Pf6N2E2_2940 0.62 -0.9 3,432,369 - Pf6N2E2_2940 0.65 -3.2 3,432,750 - -1.6 3,432,782 + -1.3 3,432,782 + -0.4 3,432,782 + -2.6 3,432,783 - +1.1 3,432,783 - +2.6 3,432,783 - -0.9 3,432,783 - -1.1 3,432,794 - +0.5 3,432,794 - +0.1 3,432,879 + -1.3 3,432,880 - -0.7 3,432,881 + -1.3 3,432,881 + -2.1 3,432,881 + -2.1 3,432,881 + -2.4 3,432,882 - +1.0 3,432,882 - -0.7 3,432,882 - +0.7 3,432,932 + -1.3 3,432,932 + -1.2 3,432,932 + -0.5 3,432,932 + +0.2 3,432,932 + -1.5 3,432,932 + -1.0 3,432,933 - -0.5 3,432,933 - -1.5 3,432,933 - +0.9 3,432,933 - -2.4 3,432,933 - +0.2 3,432,933 - +0.1 3,432,933 - -0.9 3,432,933 - +0.4 3,432,933 - -0.9 3,432,936 + -1.5 3,432,936 + -0.5 3,432,937 - -1.0 3,432,937 - -0.1 3,432,937 - +0.1 3,432,937 - +0.8 3,433,048 - Pf6N2E2_2941 0.15 -0.2 3,433,048 - Pf6N2E2_2941 0.15 +0.5 3,433,062 - Pf6N2E2_2941 0.16 +1.0 3,433,200 + Pf6N2E2_2941 0.28 -0.1 3,433,542 + Pf6N2E2_2941 0.58 -0.6 3,433,543 - Pf6N2E2_2941 0.58 -0.7 3,433,557 + Pf6N2E2_2941 0.59 -0.3 3,433,558 - Pf6N2E2_2941 0.59 -0.9 3,433,629 + Pf6N2E2_2941 0.66 -1.0 3,433,629 + Pf6N2E2_2941 0.66 +0.2 3,433,629 + Pf6N2E2_2941 0.66 -0.5 3,433,629 + Pf6N2E2_2941 0.66 +0.7
Or see this region's nucleotide sequence