Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2797 and Pf6N2E2_2798 overlap by 11 nucleotides Pf6N2E2_2798 and Pf6N2E2_2799 are separated by 29 nucleotides Pf6N2E2_2799 and Pf6N2E2_2800 are separated by 257 nucleotides Pf6N2E2_2800 and Pf6N2E2_2801 are separated by 13 nucleotides
Pf6N2E2_2797 - Prevent host death protein, Phd antitoxin, at 3,284,585 to 3,284,824
_2797
Pf6N2E2_2798 - Death on curing protein, Doc toxin, at 3,284,814 to 3,285,098
_2798
Pf6N2E2_2799 - Fosfomycin resistance protein FosA, at 3,285,128 to 3,285,541
_2799
Pf6N2E2_2800 - 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29), at 3,285,799 to 3,287,034
_2800
Pf6N2E2_2801 - Bicyclomycin resistance protein, at 3,287,048 to 3,288,211
_2801
Position (kb)
3285
3286
3287
3288 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3284.869 kb on - strand, within Pf6N2E2_2798 at 3284.869 kb on - strand, within Pf6N2E2_2798 at 3284.869 kb on - strand, within Pf6N2E2_2798 at 3284.869 kb on - strand, within Pf6N2E2_2798 at 3284.869 kb on - strand, within Pf6N2E2_2798 at 3284.871 kb on - strand, within Pf6N2E2_2798 at 3284.871 kb on - strand, within Pf6N2E2_2798 at 3284.871 kb on - strand, within Pf6N2E2_2798 at 3285.137 kb on - strand at 3285.137 kb on - strand at 3285.137 kb on - strand at 3285.156 kb on + strand at 3285.156 kb on + strand at 3285.206 kb on + strand, within Pf6N2E2_2799 at 3285.206 kb on + strand, within Pf6N2E2_2799 at 3285.206 kb on + strand, within Pf6N2E2_2799 at 3285.207 kb on - strand, within Pf6N2E2_2799 at 3285.207 kb on - strand, within Pf6N2E2_2799 at 3285.207 kb on - strand, within Pf6N2E2_2799 at 3285.207 kb on - strand, within Pf6N2E2_2799 at 3285.207 kb on - strand, within Pf6N2E2_2799 at 3285.207 kb on - strand, within Pf6N2E2_2799 at 3285.207 kb on - strand, within Pf6N2E2_2799 at 3285.207 kb on - strand, within Pf6N2E2_2799 at 3285.345 kb on + strand, within Pf6N2E2_2799 at 3285.354 kb on + strand, within Pf6N2E2_2799 at 3285.354 kb on + strand, within Pf6N2E2_2799 at 3285.355 kb on - strand, within Pf6N2E2_2799 at 3285.426 kb on + strand, within Pf6N2E2_2799 at 3285.427 kb on - strand, within Pf6N2E2_2799 at 3285.427 kb on - strand, within Pf6N2E2_2799 at 3285.464 kb on + strand, within Pf6N2E2_2799 at 3285.465 kb on - strand, within Pf6N2E2_2799 at 3285.465 kb on - strand, within Pf6N2E2_2799 at 3285.467 kb on + strand, within Pf6N2E2_2799 at 3285.467 kb on + strand, within Pf6N2E2_2799 at 3285.467 kb on + strand, within Pf6N2E2_2799 at 3285.468 kb on - strand, within Pf6N2E2_2799 at 3285.954 kb on + strand, within Pf6N2E2_2800 at 3285.972 kb on + strand, within Pf6N2E2_2800 at 3285.983 kb on - strand, within Pf6N2E2_2800 at 3285.997 kb on + strand, within Pf6N2E2_2800 at 3285.998 kb on - strand, within Pf6N2E2_2800 at 3286.102 kb on + strand, within Pf6N2E2_2800 at 3286.252 kb on + strand, within Pf6N2E2_2800 at 3286.366 kb on + strand, within Pf6N2E2_2800 at 3286.366 kb on + strand, within Pf6N2E2_2800 at 3286.366 kb on + strand, within Pf6N2E2_2800 at 3286.367 kb on - strand, within Pf6N2E2_2800 at 3286.385 kb on - strand, within Pf6N2E2_2800 at 3286.423 kb on + strand, within Pf6N2E2_2800 at 3286.423 kb on + strand, within Pf6N2E2_2800 at 3286.423 kb on + strand, within Pf6N2E2_2800 at 3286.423 kb on + strand, within Pf6N2E2_2800 at 3286.424 kb on - strand, within Pf6N2E2_2800 at 3286.424 kb on - strand, within Pf6N2E2_2800 at 3286.424 kb on - strand, within Pf6N2E2_2800 at 3286.424 kb on - strand, within Pf6N2E2_2800 at 3286.424 kb on - strand, within Pf6N2E2_2800 at 3286.700 kb on + strand, within Pf6N2E2_2800 at 3286.752 kb on + strand, within Pf6N2E2_2800 at 3286.752 kb on + strand, within Pf6N2E2_2800 at 3286.752 kb on + strand, within Pf6N2E2_2800 at 3286.752 kb on + strand, within Pf6N2E2_2800 at 3286.752 kb on + strand, within Pf6N2E2_2800 at 3286.752 kb on + strand, within Pf6N2E2_2800 at 3286.753 kb on - strand, within Pf6N2E2_2800 at 3286.753 kb on - strand, within Pf6N2E2_2800 at 3286.753 kb on - strand, within Pf6N2E2_2800 at 3286.753 kb on - strand, within Pf6N2E2_2800 at 3286.753 kb on - strand, within Pf6N2E2_2800 at 3286.753 kb on - strand, within Pf6N2E2_2800 at 3286.753 kb on - strand, within Pf6N2E2_2800 at 3286.879 kb on + strand, within Pf6N2E2_2800 at 3286.879 kb on + strand, within Pf6N2E2_2800 at 3287.059 kb on + strand at 3287.059 kb on + strand at 3287.059 kb on + strand at 3287.059 kb on + strand at 3287.060 kb on - strand at 3287.060 kb on - strand at 3287.060 kb on - strand at 3287.084 kb on - strand at 3287.084 kb on - strand at 3287.117 kb on + strand at 3287.118 kb on - strand at 3287.118 kb on - strand at 3287.118 kb on - strand at 3287.192 kb on + strand, within Pf6N2E2_2801 at 3287.192 kb on + strand, within Pf6N2E2_2801 at 3287.193 kb on - strand, within Pf6N2E2_2801 at 3287.285 kb on + strand, within Pf6N2E2_2801 at 3287.285 kb on + strand, within Pf6N2E2_2801 at 3287.286 kb on - strand, within Pf6N2E2_2801 at 3287.486 kb on + strand, within Pf6N2E2_2801 at 3287.486 kb on + strand, within Pf6N2E2_2801 at 3287.487 kb on - strand, within Pf6N2E2_2801 at 3287.636 kb on + strand, within Pf6N2E2_2801 at 3287.637 kb on - strand, within Pf6N2E2_2801 at 3287.637 kb on - strand, within Pf6N2E2_2801 at 3287.771 kb on + strand, within Pf6N2E2_2801 at 3287.771 kb on + strand, within Pf6N2E2_2801 at 3287.772 kb on - strand, within Pf6N2E2_2801 at 3287.804 kb on + strand, within Pf6N2E2_2801 at 3287.804 kb on + strand, within Pf6N2E2_2801 at 3287.804 kb on + strand, within Pf6N2E2_2801 at 3287.804 kb on + strand, within Pf6N2E2_2801 at 3287.805 kb on - strand, within Pf6N2E2_2801 at 3287.838 kb on + strand, within Pf6N2E2_2801 at 3287.839 kb on - strand, within Pf6N2E2_2801 at 3287.993 kb on + strand, within Pf6N2E2_2801 at 3287.993 kb on + strand, within Pf6N2E2_2801 at 3287.993 kb on + strand, within Pf6N2E2_2801 at 3287.994 kb on - strand, within Pf6N2E2_2801
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 3,284,869 - Pf6N2E2_2798 0.19 +0.1 3,284,869 - Pf6N2E2_2798 0.19 -0.1 3,284,869 - Pf6N2E2_2798 0.19 -0.9 3,284,869 - Pf6N2E2_2798 0.19 +0.0 3,284,869 - Pf6N2E2_2798 0.19 +1.7 3,284,871 - Pf6N2E2_2798 0.20 -0.4 3,284,871 - Pf6N2E2_2798 0.20 +0.7 3,284,871 - Pf6N2E2_2798 0.20 +0.1 3,285,137 - -1.8 3,285,137 - -1.2 3,285,137 - -0.9 3,285,156 + +1.0 3,285,156 + -0.1 3,285,206 + Pf6N2E2_2799 0.19 +0.5 3,285,206 + Pf6N2E2_2799 0.19 +0.3 3,285,206 + Pf6N2E2_2799 0.19 +0.4 3,285,207 - Pf6N2E2_2799 0.19 +0.4 3,285,207 - Pf6N2E2_2799 0.19 +0.4 3,285,207 - Pf6N2E2_2799 0.19 -0.5 3,285,207 - Pf6N2E2_2799 0.19 -0.3 3,285,207 - Pf6N2E2_2799 0.19 +1.1 3,285,207 - Pf6N2E2_2799 0.19 +0.5 3,285,207 - Pf6N2E2_2799 0.19 +0.7 3,285,207 - Pf6N2E2_2799 0.19 +1.3 3,285,345 + Pf6N2E2_2799 0.52 +0.2 3,285,354 + Pf6N2E2_2799 0.55 -0.2 3,285,354 + Pf6N2E2_2799 0.55 -0.7 3,285,355 - Pf6N2E2_2799 0.55 +0.4 3,285,426 + Pf6N2E2_2799 0.72 -0.2 3,285,427 - Pf6N2E2_2799 0.72 +1.1 3,285,427 - Pf6N2E2_2799 0.72 +0.6 3,285,464 + Pf6N2E2_2799 0.81 +1.2 3,285,465 - Pf6N2E2_2799 0.81 +0.5 3,285,465 - Pf6N2E2_2799 0.81 -0.7 3,285,467 + Pf6N2E2_2799 0.82 -0.0 3,285,467 + Pf6N2E2_2799 0.82 +0.8 3,285,467 + Pf6N2E2_2799 0.82 +1.3 3,285,468 - Pf6N2E2_2799 0.82 +0.0 3,285,954 + Pf6N2E2_2800 0.13 +1.7 3,285,972 + Pf6N2E2_2800 0.14 -0.4 3,285,983 - Pf6N2E2_2800 0.15 +0.2 3,285,997 + Pf6N2E2_2800 0.16 +0.6 3,285,998 - Pf6N2E2_2800 0.16 +0.5 3,286,102 + Pf6N2E2_2800 0.25 -0.1 3,286,252 + Pf6N2E2_2800 0.37 +1.6 3,286,366 + Pf6N2E2_2800 0.46 -0.2 3,286,366 + Pf6N2E2_2800 0.46 +0.4 3,286,366 + Pf6N2E2_2800 0.46 -2.0 3,286,367 - Pf6N2E2_2800 0.46 +0.2 3,286,385 - Pf6N2E2_2800 0.47 +0.1 3,286,423 + Pf6N2E2_2800 0.50 +1.4 3,286,423 + Pf6N2E2_2800 0.50 -0.6 3,286,423 + Pf6N2E2_2800 0.50 +2.2 3,286,423 + Pf6N2E2_2800 0.50 +1.0 3,286,424 - Pf6N2E2_2800 0.51 -0.5 3,286,424 - Pf6N2E2_2800 0.51 +1.3 3,286,424 - Pf6N2E2_2800 0.51 +0.2 3,286,424 - Pf6N2E2_2800 0.51 -0.2 3,286,424 - Pf6N2E2_2800 0.51 +0.7 3,286,700 + Pf6N2E2_2800 0.73 -0.2 3,286,752 + Pf6N2E2_2800 0.77 +0.7 3,286,752 + Pf6N2E2_2800 0.77 -0.8 3,286,752 + Pf6N2E2_2800 0.77 -0.4 3,286,752 + Pf6N2E2_2800 0.77 +1.2 3,286,752 + Pf6N2E2_2800 0.77 -0.2 3,286,752 + Pf6N2E2_2800 0.77 +0.1 3,286,753 - Pf6N2E2_2800 0.77 +0.2 3,286,753 - Pf6N2E2_2800 0.77 -0.4 3,286,753 - Pf6N2E2_2800 0.77 +0.4 3,286,753 - Pf6N2E2_2800 0.77 -0.2 3,286,753 - Pf6N2E2_2800 0.77 -0.4 3,286,753 - Pf6N2E2_2800 0.77 -0.6 3,286,753 - Pf6N2E2_2800 0.77 -0.3 3,286,879 + Pf6N2E2_2800 0.87 -0.0 3,286,879 + Pf6N2E2_2800 0.87 -1.1 3,287,059 + -0.6 3,287,059 + -1.8 3,287,059 + -0.2 3,287,059 + +0.4 3,287,060 - -0.6 3,287,060 - +0.9 3,287,060 - -0.4 3,287,084 - +0.1 3,287,084 - +0.1 3,287,117 + -0.6 3,287,118 - -0.9 3,287,118 - +0.6 3,287,118 - +0.5 3,287,192 + Pf6N2E2_2801 0.12 -0.1 3,287,192 + Pf6N2E2_2801 0.12 +1.1 3,287,193 - Pf6N2E2_2801 0.12 +0.1 3,287,285 + Pf6N2E2_2801 0.20 +0.8 3,287,285 + Pf6N2E2_2801 0.20 +0.1 3,287,286 - Pf6N2E2_2801 0.20 +0.0 3,287,486 + Pf6N2E2_2801 0.38 -0.5 3,287,486 + Pf6N2E2_2801 0.38 -0.6 3,287,487 - Pf6N2E2_2801 0.38 -0.4 3,287,636 + Pf6N2E2_2801 0.51 +0.8 3,287,637 - Pf6N2E2_2801 0.51 -0.6 3,287,637 - Pf6N2E2_2801 0.51 -0.0 3,287,771 + Pf6N2E2_2801 0.62 +1.5 3,287,771 + Pf6N2E2_2801 0.62 +0.7 3,287,772 - Pf6N2E2_2801 0.62 -0.4 3,287,804 + Pf6N2E2_2801 0.65 -0.1 3,287,804 + Pf6N2E2_2801 0.65 +1.2 3,287,804 + Pf6N2E2_2801 0.65 +0.3 3,287,804 + Pf6N2E2_2801 0.65 -0.5 3,287,805 - Pf6N2E2_2801 0.65 +0.1 3,287,838 + Pf6N2E2_2801 0.68 +0.1 3,287,839 - Pf6N2E2_2801 0.68 +0.9 3,287,993 + Pf6N2E2_2801 0.81 +0.2 3,287,993 + Pf6N2E2_2801 0.81 -0.6 3,287,993 + Pf6N2E2_2801 0.81 +0.8 3,287,994 - Pf6N2E2_2801 0.81 +0.9
Or see this region's nucleotide sequence