Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2771 and Pf6N2E2_2772 are separated by 244 nucleotides Pf6N2E2_2772 and Pf6N2E2_2773 are separated by 131 nucleotides
Pf6N2E2_2771 - Ammonia monooxygenase, at 3,259,231 to 3,260,271
_2771
Pf6N2E2_2772 - Uracil-DNA glycosylase, family 1, at 3,260,516 to 3,261,208
_2772
Pf6N2E2_2773 - Enoyl-CoA hydratase (EC 4.2.1.17), at 3,261,340 to 3,262,452
_2773
Position (kb)
3260
3261
3262 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3259.647 kb on + strand, within Pf6N2E2_2771 at 3259.648 kb on - strand, within Pf6N2E2_2771 at 3259.648 kb on - strand, within Pf6N2E2_2771 at 3259.688 kb on + strand, within Pf6N2E2_2771 at 3259.689 kb on - strand, within Pf6N2E2_2771 at 3259.689 kb on - strand, within Pf6N2E2_2771 at 3259.717 kb on + strand, within Pf6N2E2_2771 at 3259.718 kb on - strand, within Pf6N2E2_2771 at 3259.718 kb on - strand, within Pf6N2E2_2771 at 3259.866 kb on - strand, within Pf6N2E2_2771 at 3259.866 kb on - strand, within Pf6N2E2_2771 at 3259.866 kb on - strand, within Pf6N2E2_2771 at 3259.866 kb on - strand, within Pf6N2E2_2771 at 3260.060 kb on + strand, within Pf6N2E2_2771 at 3260.090 kb on + strand, within Pf6N2E2_2771 at 3260.090 kb on + strand, within Pf6N2E2_2771 at 3260.091 kb on - strand, within Pf6N2E2_2771 at 3260.091 kb on - strand, within Pf6N2E2_2771 at 3260.091 kb on - strand, within Pf6N2E2_2771 at 3260.091 kb on - strand, within Pf6N2E2_2771 at 3260.091 kb on - strand, within Pf6N2E2_2771 at 3260.091 kb on - strand, within Pf6N2E2_2771 at 3260.109 kb on + strand, within Pf6N2E2_2771 at 3260.110 kb on - strand, within Pf6N2E2_2771 at 3260.267 kb on + strand at 3260.267 kb on + strand at 3260.267 kb on + strand at 3260.267 kb on + strand at 3260.268 kb on - strand at 3260.268 kb on - strand at 3260.269 kb on + strand at 3260.269 kb on + strand at 3260.270 kb on - strand at 3260.517 kb on + strand at 3260.517 kb on + strand at 3260.517 kb on + strand at 3260.517 kb on + strand at 3260.518 kb on - strand at 3260.705 kb on + strand, within Pf6N2E2_2772 at 3260.821 kb on + strand, within Pf6N2E2_2772 at 3260.821 kb on + strand, within Pf6N2E2_2772 at 3260.821 kb on + strand, within Pf6N2E2_2772 at 3260.822 kb on - strand, within Pf6N2E2_2772 at 3260.822 kb on - strand, within Pf6N2E2_2772 at 3260.913 kb on + strand, within Pf6N2E2_2772 at 3260.913 kb on + strand, within Pf6N2E2_2772 at 3260.913 kb on + strand, within Pf6N2E2_2772 at 3260.913 kb on + strand, within Pf6N2E2_2772 at 3260.914 kb on - strand, within Pf6N2E2_2772 at 3260.914 kb on - strand, within Pf6N2E2_2772 at 3260.914 kb on - strand, within Pf6N2E2_2772 at 3260.914 kb on - strand, within Pf6N2E2_2772 at 3260.917 kb on + strand, within Pf6N2E2_2772 at 3261.100 kb on - strand, within Pf6N2E2_2772 at 3261.100 kb on - strand, within Pf6N2E2_2772 at 3261.111 kb on - strand, within Pf6N2E2_2772 at 3261.135 kb on + strand, within Pf6N2E2_2772 at 3261.135 kb on + strand, within Pf6N2E2_2772 at 3261.135 kb on + strand, within Pf6N2E2_2772 at 3261.135 kb on + strand, within Pf6N2E2_2772 at 3261.135 kb on + strand, within Pf6N2E2_2772 at 3261.136 kb on - strand, within Pf6N2E2_2772 at 3261.136 kb on - strand, within Pf6N2E2_2772 at 3261.136 kb on - strand, within Pf6N2E2_2772 at 3261.136 kb on - strand, within Pf6N2E2_2772 at 3261.191 kb on - strand at 3261.251 kb on + strand at 3261.251 kb on + strand at 3261.251 kb on + strand at 3261.252 kb on - strand at 3261.252 kb on - strand at 3261.252 kb on - strand at 3261.252 kb on - strand at 3261.252 kb on - strand at 3261.307 kb on + strand at 3261.308 kb on - strand at 3261.314 kb on + strand at 3261.314 kb on + strand at 3261.315 kb on - strand at 3261.315 kb on - strand at 3261.315 kb on - strand at 3261.503 kb on + strand, within Pf6N2E2_2773 at 3261.503 kb on + strand, within Pf6N2E2_2773 at 3261.503 kb on + strand, within Pf6N2E2_2773 at 3261.561 kb on - strand, within Pf6N2E2_2773 at 3261.590 kb on + strand, within Pf6N2E2_2773 at 3261.625 kb on + strand, within Pf6N2E2_2773 at 3261.652 kb on + strand, within Pf6N2E2_2773 at 3261.653 kb on - strand, within Pf6N2E2_2773 at 3261.685 kb on + strand, within Pf6N2E2_2773 at 3261.685 kb on + strand, within Pf6N2E2_2773 at 3261.685 kb on + strand, within Pf6N2E2_2773 at 3261.685 kb on + strand, within Pf6N2E2_2773 at 3261.685 kb on + strand, within Pf6N2E2_2773 at 3261.685 kb on + strand, within Pf6N2E2_2773 at 3261.686 kb on - strand, within Pf6N2E2_2773 at 3261.686 kb on - strand, within Pf6N2E2_2773 at 3261.686 kb on - strand, within Pf6N2E2_2773 at 3261.689 kb on + strand at 3261.689 kb on + strand, within Pf6N2E2_2773 at 3261.689 kb on + strand, within Pf6N2E2_2773 at 3261.690 kb on - strand, within Pf6N2E2_2773 at 3261.690 kb on - strand, within Pf6N2E2_2773 at 3261.690 kb on - strand, within Pf6N2E2_2773 at 3261.727 kb on - strand, within Pf6N2E2_2773 at 3262.196 kb on - strand, within Pf6N2E2_2773
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 3,259,647 + Pf6N2E2_2771 0.40 +0.0 3,259,648 - Pf6N2E2_2771 0.40 -0.2 3,259,648 - Pf6N2E2_2771 0.40 +1.0 3,259,688 + Pf6N2E2_2771 0.44 -0.3 3,259,689 - Pf6N2E2_2771 0.44 +0.1 3,259,689 - Pf6N2E2_2771 0.44 -0.8 3,259,717 + Pf6N2E2_2771 0.47 +0.2 3,259,718 - Pf6N2E2_2771 0.47 -0.2 3,259,718 - Pf6N2E2_2771 0.47 +1.5 3,259,866 - Pf6N2E2_2771 0.61 -2.4 3,259,866 - Pf6N2E2_2771 0.61 -0.0 3,259,866 - Pf6N2E2_2771 0.61 +0.2 3,259,866 - Pf6N2E2_2771 0.61 -0.1 3,260,060 + Pf6N2E2_2771 0.80 +0.8 3,260,090 + Pf6N2E2_2771 0.83 +0.5 3,260,090 + Pf6N2E2_2771 0.83 -0.8 3,260,091 - Pf6N2E2_2771 0.83 -0.4 3,260,091 - Pf6N2E2_2771 0.83 +0.3 3,260,091 - Pf6N2E2_2771 0.83 -0.7 3,260,091 - Pf6N2E2_2771 0.83 +0.2 3,260,091 - Pf6N2E2_2771 0.83 +0.6 3,260,091 - Pf6N2E2_2771 0.83 -0.0 3,260,109 + Pf6N2E2_2771 0.84 +1.3 3,260,110 - Pf6N2E2_2771 0.84 -1.3 3,260,267 + -1.0 3,260,267 + +0.6 3,260,267 + +1.5 3,260,267 + -0.9 3,260,268 - +0.5 3,260,268 - +1.1 3,260,269 + +0.3 3,260,269 + +0.1 3,260,270 - -1.2 3,260,517 + -0.2 3,260,517 + -0.1 3,260,517 + +1.4 3,260,517 + +0.9 3,260,518 - +0.3 3,260,705 + Pf6N2E2_2772 0.27 -0.5 3,260,821 + Pf6N2E2_2772 0.44 -0.3 3,260,821 + Pf6N2E2_2772 0.44 -0.8 3,260,821 + Pf6N2E2_2772 0.44 -0.2 3,260,822 - Pf6N2E2_2772 0.44 +0.7 3,260,822 - Pf6N2E2_2772 0.44 -0.6 3,260,913 + Pf6N2E2_2772 0.57 +0.6 3,260,913 + Pf6N2E2_2772 0.57 -1.2 3,260,913 + Pf6N2E2_2772 0.57 +1.1 3,260,913 + Pf6N2E2_2772 0.57 +0.3 3,260,914 - Pf6N2E2_2772 0.57 +0.7 3,260,914 - Pf6N2E2_2772 0.57 -0.2 3,260,914 - Pf6N2E2_2772 0.57 -0.6 3,260,914 - Pf6N2E2_2772 0.57 +0.8 3,260,917 + Pf6N2E2_2772 0.58 -0.2 3,261,100 - Pf6N2E2_2772 0.84 +0.4 3,261,100 - Pf6N2E2_2772 0.84 +0.3 3,261,111 - Pf6N2E2_2772 0.86 +0.8 3,261,135 + Pf6N2E2_2772 0.89 +1.4 3,261,135 + Pf6N2E2_2772 0.89 +0.6 3,261,135 + Pf6N2E2_2772 0.89 -0.4 3,261,135 + Pf6N2E2_2772 0.89 +0.2 3,261,135 + Pf6N2E2_2772 0.89 -1.0 3,261,136 - Pf6N2E2_2772 0.89 -0.3 3,261,136 - Pf6N2E2_2772 0.89 +0.2 3,261,136 - Pf6N2E2_2772 0.89 +0.2 3,261,136 - Pf6N2E2_2772 0.89 +0.8 3,261,191 - +0.4 3,261,251 + +0.3 3,261,251 + +0.3 3,261,251 + +0.1 3,261,252 - +1.2 3,261,252 - -0.9 3,261,252 - -0.6 3,261,252 - +0.9 3,261,252 - -0.5 3,261,307 + +0.8 3,261,308 - +0.3 3,261,314 + +0.6 3,261,314 + +0.3 3,261,315 - -0.2 3,261,315 - -1.6 3,261,315 - +0.0 3,261,503 + Pf6N2E2_2773 0.15 -0.2 3,261,503 + Pf6N2E2_2773 0.15 +0.4 3,261,503 + Pf6N2E2_2773 0.15 -0.0 3,261,561 - Pf6N2E2_2773 0.20 -0.7 3,261,590 + Pf6N2E2_2773 0.22 +0.5 3,261,625 + Pf6N2E2_2773 0.26 -0.5 3,261,652 + Pf6N2E2_2773 0.28 +0.2 3,261,653 - Pf6N2E2_2773 0.28 +1.0 3,261,685 + Pf6N2E2_2773 0.31 -1.1 3,261,685 + Pf6N2E2_2773 0.31 +2.2 3,261,685 + Pf6N2E2_2773 0.31 -0.6 3,261,685 + Pf6N2E2_2773 0.31 +1.8 3,261,685 + Pf6N2E2_2773 0.31 +0.6 3,261,685 + Pf6N2E2_2773 0.31 +0.1 3,261,686 - Pf6N2E2_2773 0.31 -0.9 3,261,686 - Pf6N2E2_2773 0.31 -0.5 3,261,686 - Pf6N2E2_2773 0.31 -1.3 3,261,689 + +0.1 3,261,689 + Pf6N2E2_2773 0.31 -0.8 3,261,689 + Pf6N2E2_2773 0.31 +0.8 3,261,690 - Pf6N2E2_2773 0.31 -0.1 3,261,690 - Pf6N2E2_2773 0.31 +0.6 3,261,690 - Pf6N2E2_2773 0.31 -0.4 3,261,727 - Pf6N2E2_2773 0.35 -0.3 3,262,196 - Pf6N2E2_2773 0.77 +0.1
Or see this region's nucleotide sequence