Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2723 and Pf6N2E2_2724 are separated by 160 nucleotides Pf6N2E2_2724 and Pf6N2E2_2725 are separated by 234 nucleotides Pf6N2E2_2725 and Pf6N2E2_2726 are separated by 126 nucleotides Pf6N2E2_2726 and Pf6N2E2_2727 overlap by 4 nucleotides
Pf6N2E2_2723 - YcgN (Fragment), at 3,215,338 to 3,215,787
_2723
Pf6N2E2_2724 - Nitroreductase family protein, at 3,215,948 to 3,216,541
_2724
Pf6N2E2_2725 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 3,216,776 to 3,217,708
_2725
Pf6N2E2_2726 - Protein YcgL, at 3,217,835 to 3,218,128
_2726
Pf6N2E2_2727 - Ribonuclease D (EC 3.1.26.3), at 3,218,125 to 3,219,258
_2727
Position (kb)
3216
3217
3218 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3215.830 kb on - strand at 3215.830 kb on - strand at 3215.905 kb on + strand at 3215.905 kb on + strand at 3215.905 kb on + strand at 3215.906 kb on - strand at 3215.906 kb on - strand at 3215.906 kb on - strand at 3215.906 kb on - strand at 3215.906 kb on - strand at 3215.941 kb on + strand at 3215.941 kb on + strand at 3215.942 kb on - strand at 3216.092 kb on + strand, within Pf6N2E2_2724 at 3216.119 kb on + strand, within Pf6N2E2_2724 at 3216.119 kb on + strand, within Pf6N2E2_2724 at 3216.404 kb on + strand, within Pf6N2E2_2724 at 3216.405 kb on - strand, within Pf6N2E2_2724 at 3216.464 kb on + strand, within Pf6N2E2_2724 at 3216.464 kb on + strand, within Pf6N2E2_2724 at 3216.540 kb on - strand at 3216.566 kb on + strand at 3216.566 kb on + strand at 3216.566 kb on + strand at 3216.567 kb on - strand at 3216.567 kb on - strand at 3216.567 kb on - strand at 3216.567 kb on - strand at 3216.574 kb on + strand at 3216.574 kb on + strand at 3216.574 kb on + strand at 3216.574 kb on + strand at 3216.575 kb on - strand at 3216.575 kb on - strand at 3216.671 kb on + strand at 3216.671 kb on + strand at 3216.672 kb on - strand at 3216.743 kb on - strand at 3216.743 kb on - strand at 3216.743 kb on - strand at 3216.743 kb on - strand at 3216.743 kb on - strand at 3217.092 kb on + strand, within Pf6N2E2_2725 at 3217.092 kb on + strand, within Pf6N2E2_2725 at 3217.092 kb on + strand, within Pf6N2E2_2725 at 3217.092 kb on + strand, within Pf6N2E2_2725 at 3217.092 kb on + strand, within Pf6N2E2_2725 at 3217.093 kb on - strand, within Pf6N2E2_2725 at 3217.137 kb on + strand, within Pf6N2E2_2725 at 3217.137 kb on + strand, within Pf6N2E2_2725 at 3217.138 kb on - strand, within Pf6N2E2_2725 at 3217.138 kb on - strand, within Pf6N2E2_2725 at 3217.138 kb on - strand, within Pf6N2E2_2725 at 3217.228 kb on - strand, within Pf6N2E2_2725 at 3217.228 kb on - strand, within Pf6N2E2_2725 at 3217.228 kb on - strand, within Pf6N2E2_2725 at 3217.228 kb on - strand, within Pf6N2E2_2725 at 3217.373 kb on + strand, within Pf6N2E2_2725 at 3217.374 kb on - strand, within Pf6N2E2_2725 at 3217.374 kb on - strand, within Pf6N2E2_2725 at 3217.398 kb on + strand, within Pf6N2E2_2725 at 3217.398 kb on + strand, within Pf6N2E2_2725 at 3217.398 kb on + strand, within Pf6N2E2_2725 at 3217.398 kb on + strand, within Pf6N2E2_2725 at 3217.398 kb on + strand, within Pf6N2E2_2725 at 3217.398 kb on + strand, within Pf6N2E2_2725 at 3217.398 kb on + strand, within Pf6N2E2_2725 at 3217.399 kb on - strand, within Pf6N2E2_2725 at 3217.399 kb on - strand, within Pf6N2E2_2725 at 3217.399 kb on - strand, within Pf6N2E2_2725 at 3217.399 kb on - strand, within Pf6N2E2_2725 at 3217.399 kb on - strand, within Pf6N2E2_2725 at 3217.399 kb on - strand, within Pf6N2E2_2725 at 3217.399 kb on - strand, within Pf6N2E2_2725 at 3217.399 kb on - strand, within Pf6N2E2_2725 at 3217.399 kb on - strand, within Pf6N2E2_2725 at 3217.399 kb on - strand, within Pf6N2E2_2725 at 3217.399 kb on - strand, within Pf6N2E2_2725 at 3217.410 kb on + strand, within Pf6N2E2_2725 at 3217.411 kb on - strand, within Pf6N2E2_2725 at 3217.411 kb on - strand, within Pf6N2E2_2725 at 3217.411 kb on - strand, within Pf6N2E2_2725 at 3217.459 kb on - strand, within Pf6N2E2_2725 at 3217.528 kb on + strand, within Pf6N2E2_2725 at 3217.528 kb on + strand, within Pf6N2E2_2725 at 3217.528 kb on + strand, within Pf6N2E2_2725 at 3217.528 kb on + strand, within Pf6N2E2_2725 at 3217.528 kb on + strand, within Pf6N2E2_2725 at 3217.747 kb on + strand at 3217.747 kb on + strand at 3217.766 kb on + strand at 3217.766 kb on + strand at 3217.884 kb on + strand, within Pf6N2E2_2726 at 3217.885 kb on - strand, within Pf6N2E2_2726 at 3217.885 kb on - strand, within Pf6N2E2_2726 at 3217.885 kb on - strand, within Pf6N2E2_2726 at 3218.073 kb on + strand, within Pf6N2E2_2726 at 3218.073 kb on + strand, within Pf6N2E2_2726 at 3218.073 kb on + strand, within Pf6N2E2_2726 at 3218.073 kb on + strand, within Pf6N2E2_2726 at 3218.073 kb on + strand, within Pf6N2E2_2726 at 3218.073 kb on + strand, within Pf6N2E2_2726 at 3218.073 kb on + strand, within Pf6N2E2_2726 at 3218.073 kb on + strand, within Pf6N2E2_2726 at 3218.073 kb on + strand, within Pf6N2E2_2726 at 3218.074 kb on - strand, within Pf6N2E2_2726 at 3218.074 kb on - strand, within Pf6N2E2_2726 at 3218.074 kb on - strand, within Pf6N2E2_2726 at 3218.074 kb on - strand, within Pf6N2E2_2726 at 3218.074 kb on - strand, within Pf6N2E2_2726 at 3218.074 kb on - strand, within Pf6N2E2_2726 at 3218.074 kb on - strand, within Pf6N2E2_2726 at 3218.074 kb on - strand, within Pf6N2E2_2726 at 3218.074 kb on - strand, within Pf6N2E2_2726 at 3218.074 kb on - strand, within Pf6N2E2_2726 at 3218.074 kb on - strand, within Pf6N2E2_2726 at 3218.079 kb on + strand, within Pf6N2E2_2726 at 3218.079 kb on + strand, within Pf6N2E2_2726 at 3218.079 kb on + strand, within Pf6N2E2_2726 at 3218.080 kb on - strand, within Pf6N2E2_2726 at 3218.080 kb on - strand, within Pf6N2E2_2726 at 3218.080 kb on - strand, within Pf6N2E2_2726 at 3218.080 kb on - strand, within Pf6N2E2_2726 at 3218.080 kb on - strand, within Pf6N2E2_2726 at 3218.106 kb on + strand at 3218.106 kb on + strand at 3218.119 kb on + strand at 3218.119 kb on + strand at 3218.119 kb on + strand at 3218.119 kb on + strand at 3218.119 kb on + strand at 3218.120 kb on - strand at 3218.497 kb on - strand, within Pf6N2E2_2727 at 3218.657 kb on + strand, within Pf6N2E2_2727 at 3218.658 kb on - strand, within Pf6N2E2_2727 at 3218.666 kb on + strand, within Pf6N2E2_2727
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 3,215,830 - -1.0 3,215,830 - +0.2 3,215,905 + -0.5 3,215,905 + +0.1 3,215,905 + -0.5 3,215,906 - -0.4 3,215,906 - +0.7 3,215,906 - +0.2 3,215,906 - -0.5 3,215,906 - +0.2 3,215,941 + -0.4 3,215,941 + +1.2 3,215,942 - +0.3 3,216,092 + Pf6N2E2_2724 0.24 +0.2 3,216,119 + Pf6N2E2_2724 0.29 -0.2 3,216,119 + Pf6N2E2_2724 0.29 -2.1 3,216,404 + Pf6N2E2_2724 0.77 +0.7 3,216,405 - Pf6N2E2_2724 0.77 -0.4 3,216,464 + Pf6N2E2_2724 0.87 -0.4 3,216,464 + Pf6N2E2_2724 0.87 +1.4 3,216,540 - +0.3 3,216,566 + +0.2 3,216,566 + -1.0 3,216,566 + +0.3 3,216,567 - -0.0 3,216,567 - +1.1 3,216,567 - -0.1 3,216,567 - -0.7 3,216,574 + -0.2 3,216,574 + -0.2 3,216,574 + -0.4 3,216,574 + -2.6 3,216,575 - -0.6 3,216,575 - +1.4 3,216,671 + -0.4 3,216,671 + -0.4 3,216,672 - -0.0 3,216,743 - -1.6 3,216,743 - -0.3 3,216,743 - -0.4 3,216,743 - -0.5 3,216,743 - +0.8 3,217,092 + Pf6N2E2_2725 0.34 -1.7 3,217,092 + Pf6N2E2_2725 0.34 -1.3 3,217,092 + Pf6N2E2_2725 0.34 +0.6 3,217,092 + Pf6N2E2_2725 0.34 -1.2 3,217,092 + Pf6N2E2_2725 0.34 +2.4 3,217,093 - Pf6N2E2_2725 0.34 -1.8 3,217,137 + Pf6N2E2_2725 0.39 +0.1 3,217,137 + Pf6N2E2_2725 0.39 +0.4 3,217,138 - Pf6N2E2_2725 0.39 -1.6 3,217,138 - Pf6N2E2_2725 0.39 -0.9 3,217,138 - Pf6N2E2_2725 0.39 -0.6 3,217,228 - Pf6N2E2_2725 0.48 -0.9 3,217,228 - Pf6N2E2_2725 0.48 -0.8 3,217,228 - Pf6N2E2_2725 0.48 -0.2 3,217,228 - Pf6N2E2_2725 0.48 -0.5 3,217,373 + Pf6N2E2_2725 0.64 -2.0 3,217,374 - Pf6N2E2_2725 0.64 -0.3 3,217,374 - Pf6N2E2_2725 0.64 -1.8 3,217,398 + Pf6N2E2_2725 0.67 -0.3 3,217,398 + Pf6N2E2_2725 0.67 -0.8 3,217,398 + Pf6N2E2_2725 0.67 -1.9 3,217,398 + Pf6N2E2_2725 0.67 -1.4 3,217,398 + Pf6N2E2_2725 0.67 -1.3 3,217,398 + Pf6N2E2_2725 0.67 -1.4 3,217,398 + Pf6N2E2_2725 0.67 -0.9 3,217,399 - Pf6N2E2_2725 0.67 -0.8 3,217,399 - Pf6N2E2_2725 0.67 -1.3 3,217,399 - Pf6N2E2_2725 0.67 -2.3 3,217,399 - Pf6N2E2_2725 0.67 -0.5 3,217,399 - Pf6N2E2_2725 0.67 -2.2 3,217,399 - Pf6N2E2_2725 0.67 -1.2 3,217,399 - Pf6N2E2_2725 0.67 -1.5 3,217,399 - Pf6N2E2_2725 0.67 -2.1 3,217,399 - Pf6N2E2_2725 0.67 -1.0 3,217,399 - Pf6N2E2_2725 0.67 -0.3 3,217,399 - Pf6N2E2_2725 0.67 -0.1 3,217,410 + Pf6N2E2_2725 0.68 -1.3 3,217,411 - Pf6N2E2_2725 0.68 -0.6 3,217,411 - Pf6N2E2_2725 0.68 +0.3 3,217,411 - Pf6N2E2_2725 0.68 -1.8 3,217,459 - Pf6N2E2_2725 0.73 -0.7 3,217,528 + Pf6N2E2_2725 0.81 -1.5 3,217,528 + Pf6N2E2_2725 0.81 -2.3 3,217,528 + Pf6N2E2_2725 0.81 -0.2 3,217,528 + Pf6N2E2_2725 0.81 -2.0 3,217,528 + Pf6N2E2_2725 0.81 -0.7 3,217,747 + -0.4 3,217,747 + -1.2 3,217,766 + -0.5 3,217,766 + -2.3 3,217,884 + Pf6N2E2_2726 0.17 +0.8 3,217,885 - Pf6N2E2_2726 0.17 +0.8 3,217,885 - Pf6N2E2_2726 0.17 -0.3 3,217,885 - Pf6N2E2_2726 0.17 +1.3 3,218,073 + Pf6N2E2_2726 0.81 -0.3 3,218,073 + Pf6N2E2_2726 0.81 -2.2 3,218,073 + Pf6N2E2_2726 0.81 -0.3 3,218,073 + Pf6N2E2_2726 0.81 +0.2 3,218,073 + Pf6N2E2_2726 0.81 -0.4 3,218,073 + Pf6N2E2_2726 0.81 +0.2 3,218,073 + Pf6N2E2_2726 0.81 -2.7 3,218,073 + Pf6N2E2_2726 0.81 +1.2 3,218,073 + Pf6N2E2_2726 0.81 -0.2 3,218,074 - Pf6N2E2_2726 0.81 +0.7 3,218,074 - Pf6N2E2_2726 0.81 +1.4 3,218,074 - Pf6N2E2_2726 0.81 +1.3 3,218,074 - Pf6N2E2_2726 0.81 +2.1 3,218,074 - Pf6N2E2_2726 0.81 +0.9 3,218,074 - Pf6N2E2_2726 0.81 +1.7 3,218,074 - Pf6N2E2_2726 0.81 -0.8 3,218,074 - Pf6N2E2_2726 0.81 +0.3 3,218,074 - Pf6N2E2_2726 0.81 +0.7 3,218,074 - Pf6N2E2_2726 0.81 +1.2 3,218,074 - Pf6N2E2_2726 0.81 +1.0 3,218,079 + Pf6N2E2_2726 0.83 -0.9 3,218,079 + Pf6N2E2_2726 0.83 -0.4 3,218,079 + Pf6N2E2_2726 0.83 -1.4 3,218,080 - Pf6N2E2_2726 0.83 +0.7 3,218,080 - Pf6N2E2_2726 0.83 +1.2 3,218,080 - Pf6N2E2_2726 0.83 +1.3 3,218,080 - Pf6N2E2_2726 0.83 +0.4 3,218,080 - Pf6N2E2_2726 0.83 +1.1 3,218,106 + -1.0 3,218,106 + -1.3 3,218,119 + +0.2 3,218,119 + -0.4 3,218,119 + -1.1 3,218,119 + -2.5 3,218,119 + -0.3 3,218,120 - +1.2 3,218,497 - Pf6N2E2_2727 0.33 +1.3 3,218,657 + Pf6N2E2_2727 0.47 +0.2 3,218,658 - Pf6N2E2_2727 0.47 +1.2 3,218,666 + Pf6N2E2_2727 0.48 -2.6
Or see this region's nucleotide sequence