Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2680 and Pf6N2E2_2681 are separated by 150 nucleotides Pf6N2E2_2681 and Pf6N2E2_2682 are separated by 50 nucleotides Pf6N2E2_2682 and Pf6N2E2_2683 are separated by 14 nucleotides
Pf6N2E2_2680 - FMN-dependent NADH-azoreductase, at 3,169,506 to 3,170,105
_2680
Pf6N2E2_2681 - 3-phosphoglycerate kinase, at 3,170,256 to 3,170,570
_2681
Pf6N2E2_2682 - Transcriptional regulator, LysR family, at 3,170,621 to 3,171,544
_2682
Pf6N2E2_2683 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 3,171,559 to 3,172,362
_2683
Position (kb)
3170
3171 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3169.296 kb on + strand at 3169.345 kb on + strand at 3169.345 kb on + strand at 3169.346 kb on - strand at 3169.352 kb on - strand at 3169.352 kb on - strand at 3169.473 kb on - strand at 3169.473 kb on - strand at 3169.639 kb on + strand, within Pf6N2E2_2680 at 3169.640 kb on - strand, within Pf6N2E2_2680 at 3169.640 kb on - strand, within Pf6N2E2_2680 at 3169.744 kb on + strand, within Pf6N2E2_2680 at 3169.745 kb on - strand, within Pf6N2E2_2680 at 3169.745 kb on - strand, within Pf6N2E2_2680 at 3169.745 kb on - strand, within Pf6N2E2_2680 at 3169.790 kb on + strand, within Pf6N2E2_2680 at 3169.790 kb on + strand, within Pf6N2E2_2680 at 3169.791 kb on - strand, within Pf6N2E2_2680 at 3169.791 kb on - strand, within Pf6N2E2_2680 at 3169.816 kb on + strand, within Pf6N2E2_2680 at 3169.816 kb on + strand, within Pf6N2E2_2680 at 3170.031 kb on - strand, within Pf6N2E2_2680 at 3170.112 kb on + strand at 3170.112 kb on + strand at 3170.112 kb on + strand at 3170.112 kb on + strand at 3170.112 kb on + strand at 3170.112 kb on + strand at 3170.113 kb on - strand at 3170.396 kb on + strand, within Pf6N2E2_2681 at 3170.396 kb on + strand, within Pf6N2E2_2681 at 3170.419 kb on + strand, within Pf6N2E2_2681 at 3170.461 kb on + strand, within Pf6N2E2_2681 at 3170.461 kb on + strand, within Pf6N2E2_2681 at 3170.462 kb on - strand, within Pf6N2E2_2681 at 3170.601 kb on + strand at 3170.601 kb on + strand at 3170.602 kb on - strand at 3170.602 kb on - strand at 3170.602 kb on - strand at 3170.735 kb on + strand, within Pf6N2E2_2682 at 3170.736 kb on - strand, within Pf6N2E2_2682 at 3170.736 kb on - strand, within Pf6N2E2_2682 at 3170.770 kb on - strand, within Pf6N2E2_2682 at 3170.937 kb on - strand, within Pf6N2E2_2682 at 3171.565 kb on + strand at 3171.566 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 3,169,296 + -1.0 3,169,345 + -2.4 3,169,345 + -2.0 3,169,346 - -0.5 3,169,352 - +1.4 3,169,352 - +1.5 3,169,473 - +0.5 3,169,473 - -1.0 3,169,639 + Pf6N2E2_2680 0.22 +0.8 3,169,640 - Pf6N2E2_2680 0.22 +0.4 3,169,640 - Pf6N2E2_2680 0.22 +0.8 3,169,744 + Pf6N2E2_2680 0.40 -1.3 3,169,745 - Pf6N2E2_2680 0.40 -0.5 3,169,745 - Pf6N2E2_2680 0.40 -0.2 3,169,745 - Pf6N2E2_2680 0.40 +0.5 3,169,790 + Pf6N2E2_2680 0.47 +0.6 3,169,790 + Pf6N2E2_2680 0.47 +0.9 3,169,791 - Pf6N2E2_2680 0.47 -0.4 3,169,791 - Pf6N2E2_2680 0.47 -0.1 3,169,816 + Pf6N2E2_2680 0.52 -0.9 3,169,816 + Pf6N2E2_2680 0.52 -0.4 3,170,031 - Pf6N2E2_2680 0.88 +0.1 3,170,112 + -2.1 3,170,112 + -0.3 3,170,112 + -0.5 3,170,112 + -3.4 3,170,112 + -2.7 3,170,112 + -0.7 3,170,113 - -0.0 3,170,396 + Pf6N2E2_2681 0.44 -2.2 3,170,396 + Pf6N2E2_2681 0.44 -1.0 3,170,419 + Pf6N2E2_2681 0.52 -0.6 3,170,461 + Pf6N2E2_2681 0.65 -2.4 3,170,461 + Pf6N2E2_2681 0.65 -2.3 3,170,462 - Pf6N2E2_2681 0.65 +2.2 3,170,601 + +0.9 3,170,601 + +1.4 3,170,602 - +0.8 3,170,602 - -1.8 3,170,602 - +0.3 3,170,735 + Pf6N2E2_2682 0.12 +0.1 3,170,736 - Pf6N2E2_2682 0.12 +1.3 3,170,736 - Pf6N2E2_2682 0.12 +0.4 3,170,770 - Pf6N2E2_2682 0.16 -1.3 3,170,937 - Pf6N2E2_2682 0.34 -0.2 3,171,565 + +1.6 3,171,566 - -0.5
Or see this region's nucleotide sequence