Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2521

Experiment: Choline Lysine 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2520 and Pf6N2E2_2521 overlap by 1 nucleotidesPf6N2E2_2521 and Pf6N2E2_2522 are separated by 298 nucleotides Pf6N2E2_2520 - Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51), at 2,974,253 to 2,975,347 _2520 Pf6N2E2_2521 - Phosphoserine aminotransferase (EC 2.6.1.52), at 2,975,347 to 2,976,438 _2521 Pf6N2E2_2522 - DNA gyrase subunit A (EC 5.99.1.3), at 2,976,737 to 2,979,400 _2522 Position (kb) 2975 2976 2977Strain fitness (log2 ratio) -1 0 1at 2976.464 kb on - strandat 2976.493 kb on - strandat 2976.493 kb on - strandat 2976.493 kb on - strandat 2976.493 kb on - strandat 2976.493 kb on - strandat 2976.493 kb on - strandat 2976.507 kb on + strandat 2976.508 kb on - strandat 2976.508 kb on - strandat 2976.508 kb on - strandat 2976.508 kb on - strandat 2976.572 kb on + strandat 2976.573 kb on - strandat 2976.573 kb on - strandat 2976.739 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline Lysine 20 mM
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2,976,464 - +0.0
2,976,493 - +0.3
2,976,493 - +0.5
2,976,493 - -1.3
2,976,493 - -0.6
2,976,493 - -0.3
2,976,493 - -0.1
2,976,507 + +0.1
2,976,508 - -0.3
2,976,508 - -0.7
2,976,508 - -1.0
2,976,508 - -0.3
2,976,572 + +0.2
2,976,573 - +0.3
2,976,573 - +0.4
2,976,739 - -0.9

Or see this region's nucleotide sequence