Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2513 and Pf6N2E2_2514 are separated by 168 nucleotides Pf6N2E2_2514 and Pf6N2E2_2515 are separated by 430 nucleotides
Pf6N2E2_2513 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 2,964,520 to 2,965,803
_2513
Pf6N2E2_2514 - regulator of length of O-antigen component of lipopolysaccharide chains, at 2,965,972 to 2,967,021
_2514
Pf6N2E2_2515 - Integration host factor beta subunit, at 2,967,452 to 2,967,748
_2515
Position (kb)
2965
2966
2967
2968 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2964.996 kb on - strand, within Pf6N2E2_2513 at 2965.015 kb on - strand, within Pf6N2E2_2513 at 2965.196 kb on - strand, within Pf6N2E2_2513 at 2965.713 kb on - strand at 2965.862 kb on - strand at 2965.865 kb on - strand at 2965.865 kb on - strand at 2965.885 kb on - strand at 2965.885 kb on - strand at 2965.939 kb on - strand at 2965.944 kb on - strand at 2966.008 kb on - strand at 2966.057 kb on - strand at 2966.108 kb on - strand, within Pf6N2E2_2514 at 2966.305 kb on - strand, within Pf6N2E2_2514 at 2966.344 kb on - strand, within Pf6N2E2_2514 at 2966.381 kb on - strand, within Pf6N2E2_2514 at 2966.479 kb on + strand, within Pf6N2E2_2514 at 2966.480 kb on - strand, within Pf6N2E2_2514 at 2966.491 kb on - strand, within Pf6N2E2_2514 at 2966.528 kb on + strand, within Pf6N2E2_2514 at 2966.585 kb on + strand, within Pf6N2E2_2514 at 2966.586 kb on - strand, within Pf6N2E2_2514 at 2966.693 kb on - strand, within Pf6N2E2_2514 at 2967.125 kb on + strand at 2967.163 kb on + strand at 2967.163 kb on + strand at 2967.164 kb on - strand at 2967.203 kb on - strand at 2967.252 kb on + strand at 2967.252 kb on + strand at 2967.257 kb on + strand at 2967.327 kb on + strand at 2967.328 kb on - strand at 2967.328 kb on - strand at 2967.328 kb on - strand at 2967.328 kb on - strand at 2967.491 kb on - strand, within Pf6N2E2_2515 at 2967.799 kb on - strand at 2967.830 kb on + strand at 2967.947 kb on - strand at 2967.992 kb on - strand at 2967.992 kb on - strand at 2968.004 kb on + strand at 2968.005 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 2,964,996 - Pf6N2E2_2513 0.37 -2.1 2,965,015 - Pf6N2E2_2513 0.39 -2.6 2,965,196 - Pf6N2E2_2513 0.53 -3.4 2,965,713 - -3.1 2,965,862 - +0.7 2,965,865 - -1.2 2,965,865 - -0.2 2,965,885 - +0.8 2,965,885 - +0.9 2,965,939 - +0.0 2,965,944 - -0.1 2,966,008 - -0.2 2,966,057 - -2.7 2,966,108 - Pf6N2E2_2514 0.13 -2.5 2,966,305 - Pf6N2E2_2514 0.32 +0.6 2,966,344 - Pf6N2E2_2514 0.35 -0.8 2,966,381 - Pf6N2E2_2514 0.39 -1.5 2,966,479 + Pf6N2E2_2514 0.48 -1.2 2,966,480 - Pf6N2E2_2514 0.48 -1.2 2,966,491 - Pf6N2E2_2514 0.49 -1.5 2,966,528 + Pf6N2E2_2514 0.53 -1.5 2,966,585 + Pf6N2E2_2514 0.58 -0.9 2,966,586 - Pf6N2E2_2514 0.58 -1.6 2,966,693 - Pf6N2E2_2514 0.69 -2.7 2,967,125 + -1.6 2,967,163 + -2.0 2,967,163 + -2.8 2,967,164 - -0.7 2,967,203 - +0.2 2,967,252 + -0.8 2,967,252 + -3.4 2,967,257 + -1.9 2,967,327 + -1.6 2,967,328 - +2.2 2,967,328 - +0.4 2,967,328 - -0.1 2,967,328 - +0.3 2,967,491 - Pf6N2E2_2515 0.13 -1.5 2,967,799 - +0.6 2,967,830 + +0.1 2,967,947 - -0.6 2,967,992 - +0.1 2,967,992 - +0.3 2,968,004 + +0.8 2,968,005 - -0.2
Or see this region's nucleotide sequence