Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2507 and Pf6N2E2_2508 are separated by 54 nucleotides Pf6N2E2_2508 and Pf6N2E2_2509 are separated by 182 nucleotides Pf6N2E2_2509 and Pf6N2E2_2510 are separated by 485 nucleotides
Pf6N2E2_2507 - Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-), at 2,960,053 to 2,960,700
_2507
Pf6N2E2_2508 - hypothetical protein, at 2,960,755 to 2,960,886
_2508
Pf6N2E2_2509 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 2,961,069 to 2,961,572
_2509
Pf6N2E2_2510 - Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase, at 2,962,058 to 2,963,188
_2510
Position (kb)
2961
2962 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2960.098 kb on + strand at 2960.185 kb on + strand, within Pf6N2E2_2507 at 2960.186 kb on - strand, within Pf6N2E2_2507 at 2960.186 kb on - strand, within Pf6N2E2_2507 at 2960.191 kb on - strand, within Pf6N2E2_2507 at 2960.252 kb on + strand, within Pf6N2E2_2507 at 2960.253 kb on - strand, within Pf6N2E2_2507 at 2960.276 kb on + strand, within Pf6N2E2_2507 at 2960.277 kb on - strand, within Pf6N2E2_2507 at 2960.279 kb on - strand, within Pf6N2E2_2507 at 2960.279 kb on - strand, within Pf6N2E2_2507 at 2960.363 kb on - strand, within Pf6N2E2_2507 at 2960.363 kb on - strand, within Pf6N2E2_2507 at 2960.363 kb on - strand, within Pf6N2E2_2507 at 2960.452 kb on - strand, within Pf6N2E2_2507 at 2960.452 kb on - strand, within Pf6N2E2_2507 at 2960.520 kb on + strand, within Pf6N2E2_2507 at 2960.521 kb on - strand, within Pf6N2E2_2507 at 2960.621 kb on + strand, within Pf6N2E2_2507 at 2960.660 kb on - strand at 2960.722 kb on + strand at 2960.723 kb on - strand at 2960.732 kb on - strand at 2960.732 kb on - strand at 2960.782 kb on + strand, within Pf6N2E2_2508 at 2960.782 kb on + strand, within Pf6N2E2_2508 at 2960.782 kb on + strand at 2960.782 kb on + strand, within Pf6N2E2_2508 at 2960.783 kb on - strand, within Pf6N2E2_2508 at 2960.783 kb on - strand, within Pf6N2E2_2508 at 2960.783 kb on - strand, within Pf6N2E2_2508 at 2960.783 kb on - strand, within Pf6N2E2_2508 at 2960.783 kb on - strand, within Pf6N2E2_2508 at 2960.783 kb on - strand, within Pf6N2E2_2508 at 2960.959 kb on - strand at 2960.959 kb on - strand at 2960.988 kb on + strand at 2960.999 kb on + strand at 2961.000 kb on - strand at 2961.000 kb on - strand at 2961.023 kb on - strand at 2961.113 kb on + strand at 2961.114 kb on - strand at 2961.217 kb on - strand, within Pf6N2E2_2509 at 2961.342 kb on - strand, within Pf6N2E2_2509 at 2961.632 kb on - strand at 2961.873 kb on - strand at 2961.972 kb on - strand at 2962.002 kb on + strand at 2962.042 kb on - strand at 2962.256 kb on + strand, within Pf6N2E2_2510 at 2962.258 kb on + strand, within Pf6N2E2_2510 at 2962.262 kb on - strand, within Pf6N2E2_2510 at 2962.364 kb on + strand, within Pf6N2E2_2510 at 2962.365 kb on - strand, within Pf6N2E2_2510 at 2962.365 kb on - strand, within Pf6N2E2_2510 at 2962.370 kb on - strand, within Pf6N2E2_2510 at 2962.370 kb on - strand, within Pf6N2E2_2510 at 2962.386 kb on - strand, within Pf6N2E2_2510 at 2962.449 kb on - strand, within Pf6N2E2_2510 at 2962.449 kb on - strand, within Pf6N2E2_2510
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 2,960,098 + -2.0 2,960,185 + Pf6N2E2_2507 0.20 -0.7 2,960,186 - Pf6N2E2_2507 0.21 -0.9 2,960,186 - Pf6N2E2_2507 0.21 -0.8 2,960,191 - Pf6N2E2_2507 0.21 -2.4 2,960,252 + Pf6N2E2_2507 0.31 -2.6 2,960,253 - Pf6N2E2_2507 0.31 -1.0 2,960,276 + Pf6N2E2_2507 0.34 -1.9 2,960,277 - Pf6N2E2_2507 0.35 -2.3 2,960,279 - Pf6N2E2_2507 0.35 -1.5 2,960,279 - Pf6N2E2_2507 0.35 -1.4 2,960,363 - Pf6N2E2_2507 0.48 -1.8 2,960,363 - Pf6N2E2_2507 0.48 -0.1 2,960,363 - Pf6N2E2_2507 0.48 -2.8 2,960,452 - Pf6N2E2_2507 0.62 -1.9 2,960,452 - Pf6N2E2_2507 0.62 -2.5 2,960,520 + Pf6N2E2_2507 0.72 -2.3 2,960,521 - Pf6N2E2_2507 0.72 -1.4 2,960,621 + Pf6N2E2_2507 0.88 -1.0 2,960,660 - -1.4 2,960,722 + +0.5 2,960,723 - -1.2 2,960,732 - -0.3 2,960,732 - -1.9 2,960,782 + Pf6N2E2_2508 0.20 -2.3 2,960,782 + Pf6N2E2_2508 0.20 -2.2 2,960,782 + -0.1 2,960,782 + Pf6N2E2_2508 0.20 -2.8 2,960,783 - Pf6N2E2_2508 0.21 -1.4 2,960,783 - Pf6N2E2_2508 0.21 -2.6 2,960,783 - Pf6N2E2_2508 0.21 -0.8 2,960,783 - Pf6N2E2_2508 0.21 -3.3 2,960,783 - Pf6N2E2_2508 0.21 -1.9 2,960,783 - Pf6N2E2_2508 0.21 -1.6 2,960,959 - -2.4 2,960,959 - +0.1 2,960,988 + -0.6 2,960,999 + -2.2 2,961,000 - -2.2 2,961,000 - -0.4 2,961,023 - -2.2 2,961,113 + -0.2 2,961,114 - +0.3 2,961,217 - Pf6N2E2_2509 0.29 -0.5 2,961,342 - Pf6N2E2_2509 0.54 -1.8 2,961,632 - -1.3 2,961,873 - -1.5 2,961,972 - -0.0 2,962,002 + -2.5 2,962,042 - -0.5 2,962,256 + Pf6N2E2_2510 0.18 -0.9 2,962,258 + Pf6N2E2_2510 0.18 -1.5 2,962,262 - Pf6N2E2_2510 0.18 -0.3 2,962,364 + Pf6N2E2_2510 0.27 -1.3 2,962,365 - Pf6N2E2_2510 0.27 -1.9 2,962,365 - Pf6N2E2_2510 0.27 -2.5 2,962,370 - Pf6N2E2_2510 0.28 -2.0 2,962,370 - Pf6N2E2_2510 0.28 -2.7 2,962,386 - Pf6N2E2_2510 0.29 -2.1 2,962,449 - Pf6N2E2_2510 0.35 -1.5 2,962,449 - Pf6N2E2_2510 0.35 -1.2
Or see this region's nucleotide sequence