Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2293 and Pf6N2E2_2294 are separated by 213 nucleotides Pf6N2E2_2294 and Pf6N2E2_2295 are separated by 118 nucleotides
Pf6N2E2_2293 - COG0488: ATPase components of ABC transporters with duplicated ATPase domains, at 2,712,997 to 2,714,907
_2293
Pf6N2E2_2294 - Soluble lytic murein transglycosylase precursor (EC 3.2.1.-), at 2,715,121 to 2,717,049
_2294
Pf6N2E2_2295 - Lipid A export ATP-binding/permease protein MsbA, at 2,717,168 to 2,718,940
_2295
Position (kb)
2715
2716
2717
2718 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2714.188 kb on + strand, within Pf6N2E2_2293 at 2714.189 kb on - strand, within Pf6N2E2_2293 at 2714.204 kb on + strand, within Pf6N2E2_2293 at 2714.204 kb on + strand, within Pf6N2E2_2293 at 2714.660 kb on - strand, within Pf6N2E2_2293 at 2714.863 kb on - strand at 2714.992 kb on - strand at 2715.056 kb on - strand at 2715.060 kb on + strand at 2715.061 kb on - strand at 2715.070 kb on + strand at 2715.106 kb on - strand at 2715.106 kb on - strand at 2715.146 kb on + strand at 2715.147 kb on - strand at 2715.162 kb on - strand at 2715.198 kb on + strand at 2715.217 kb on + strand at 2715.221 kb on - strand at 2715.221 kb on - strand at 2715.265 kb on + strand at 2715.265 kb on + strand at 2715.310 kb on + strand at 2715.310 kb on + strand at 2715.310 kb on + strand at 2715.319 kb on + strand, within Pf6N2E2_2294 at 2715.472 kb on + strand, within Pf6N2E2_2294 at 2715.472 kb on + strand, within Pf6N2E2_2294 at 2715.472 kb on + strand, within Pf6N2E2_2294 at 2715.473 kb on - strand, within Pf6N2E2_2294 at 2715.473 kb on - strand, within Pf6N2E2_2294 at 2715.473 kb on - strand, within Pf6N2E2_2294 at 2715.473 kb on - strand, within Pf6N2E2_2294 at 2715.473 kb on - strand, within Pf6N2E2_2294 at 2715.473 kb on - strand, within Pf6N2E2_2294 at 2715.476 kb on - strand, within Pf6N2E2_2294 at 2715.476 kb on - strand, within Pf6N2E2_2294 at 2715.556 kb on + strand, within Pf6N2E2_2294 at 2715.557 kb on - strand, within Pf6N2E2_2294 at 2715.626 kb on + strand, within Pf6N2E2_2294 at 2715.627 kb on - strand, within Pf6N2E2_2294 at 2715.700 kb on + strand, within Pf6N2E2_2294 at 2715.700 kb on + strand, within Pf6N2E2_2294 at 2715.701 kb on - strand, within Pf6N2E2_2294 at 2716.018 kb on + strand, within Pf6N2E2_2294 at 2716.019 kb on - strand, within Pf6N2E2_2294 at 2716.019 kb on - strand, within Pf6N2E2_2294 at 2716.167 kb on + strand, within Pf6N2E2_2294 at 2716.213 kb on + strand, within Pf6N2E2_2294 at 2716.214 kb on - strand, within Pf6N2E2_2294 at 2716.214 kb on - strand, within Pf6N2E2_2294 at 2716.214 kb on - strand, within Pf6N2E2_2294 at 2716.214 kb on - strand, within Pf6N2E2_2294 at 2716.214 kb on - strand, within Pf6N2E2_2294 at 2716.243 kb on + strand, within Pf6N2E2_2294 at 2716.279 kb on + strand, within Pf6N2E2_2294 at 2716.408 kb on + strand, within Pf6N2E2_2294 at 2716.409 kb on - strand, within Pf6N2E2_2294 at 2716.466 kb on - strand, within Pf6N2E2_2294 at 2716.466 kb on - strand, within Pf6N2E2_2294 at 2716.480 kb on + strand, within Pf6N2E2_2294 at 2716.480 kb on + strand, within Pf6N2E2_2294 at 2716.481 kb on - strand, within Pf6N2E2_2294 at 2716.516 kb on + strand, within Pf6N2E2_2294 at 2716.516 kb on + strand, within Pf6N2E2_2294 at 2716.517 kb on - strand, within Pf6N2E2_2294 at 2716.517 kb on - strand, within Pf6N2E2_2294 at 2716.517 kb on - strand, within Pf6N2E2_2294 at 2716.517 kb on - strand, within Pf6N2E2_2294 at 2716.517 kb on - strand, within Pf6N2E2_2294 at 2716.949 kb on - strand at 2716.949 kb on - strand at 2716.949 kb on - strand at 2716.949 kb on - strand at 2716.970 kb on - strand at 2716.981 kb on + strand at 2716.981 kb on + strand at 2716.982 kb on - strand at 2716.982 kb on - strand at 2716.982 kb on - strand at 2716.982 kb on - strand at 2717.032 kb on + strand at 2717.032 kb on + strand at 2717.032 kb on + strand at 2717.122 kb on + strand at 2717.223 kb on + strand at 2717.223 kb on + strand at 2717.224 kb on - strand at 2717.626 kb on + strand, within Pf6N2E2_2295 at 2717.627 kb on - strand, within Pf6N2E2_2295 at 2717.627 kb on - strand, within Pf6N2E2_2295 at 2717.627 kb on - strand, within Pf6N2E2_2295 at 2717.627 kb on - strand, within Pf6N2E2_2295 at 2717.627 kb on - strand, within Pf6N2E2_2295 at 2717.757 kb on + strand, within Pf6N2E2_2295 at 2717.757 kb on + strand, within Pf6N2E2_2295 at 2717.758 kb on - strand, within Pf6N2E2_2295 at 2717.758 kb on - strand, within Pf6N2E2_2295 at 2717.758 kb on - strand, within Pf6N2E2_2295 at 2717.758 kb on - strand, within Pf6N2E2_2295 at 2717.758 kb on - strand, within Pf6N2E2_2295 at 2717.758 kb on - strand, within Pf6N2E2_2295 at 2717.758 kb on - strand, within Pf6N2E2_2295 at 2717.758 kb on - strand, within Pf6N2E2_2295 at 2717.778 kb on + strand, within Pf6N2E2_2295 at 2717.778 kb on + strand, within Pf6N2E2_2295 at 2717.778 kb on + strand, within Pf6N2E2_2295 at 2717.778 kb on + strand, within Pf6N2E2_2295 at 2717.778 kb on + strand, within Pf6N2E2_2295 at 2717.778 kb on + strand, within Pf6N2E2_2295 at 2717.779 kb on - strand, within Pf6N2E2_2295 at 2717.779 kb on - strand, within Pf6N2E2_2295 at 2717.779 kb on - strand, within Pf6N2E2_2295 at 2717.865 kb on + strand, within Pf6N2E2_2295 at 2717.865 kb on + strand, within Pf6N2E2_2295 at 2717.866 kb on - strand, within Pf6N2E2_2295 at 2717.988 kb on - strand, within Pf6N2E2_2295
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 2,714,188 + Pf6N2E2_2293 0.62 +1.1 2,714,189 - Pf6N2E2_2293 0.62 -0.3 2,714,204 + Pf6N2E2_2293 0.63 -0.1 2,714,204 + Pf6N2E2_2293 0.63 +1.0 2,714,660 - Pf6N2E2_2293 0.87 +1.3 2,714,863 - -0.2 2,714,992 - +1.1 2,715,056 - +0.0 2,715,060 + +1.0 2,715,061 - -1.0 2,715,070 + -0.4 2,715,106 - -0.6 2,715,106 - -3.2 2,715,146 + -0.4 2,715,147 - -0.1 2,715,162 - -1.0 2,715,198 + -0.9 2,715,217 + -0.7 2,715,221 - -0.3 2,715,221 - -0.4 2,715,265 + -1.5 2,715,265 + -0.3 2,715,310 + -0.8 2,715,310 + -0.0 2,715,310 + -0.3 2,715,319 + Pf6N2E2_2294 0.10 +0.1 2,715,472 + Pf6N2E2_2294 0.18 -1.2 2,715,472 + Pf6N2E2_2294 0.18 -0.1 2,715,472 + Pf6N2E2_2294 0.18 -0.8 2,715,473 - Pf6N2E2_2294 0.18 -1.0 2,715,473 - Pf6N2E2_2294 0.18 -0.9 2,715,473 - Pf6N2E2_2294 0.18 -2.2 2,715,473 - Pf6N2E2_2294 0.18 -2.4 2,715,473 - Pf6N2E2_2294 0.18 -0.6 2,715,473 - Pf6N2E2_2294 0.18 +0.4 2,715,476 - Pf6N2E2_2294 0.18 +1.5 2,715,476 - Pf6N2E2_2294 0.18 -0.2 2,715,556 + Pf6N2E2_2294 0.23 -0.7 2,715,557 - Pf6N2E2_2294 0.23 -0.2 2,715,626 + Pf6N2E2_2294 0.26 +0.2 2,715,627 - Pf6N2E2_2294 0.26 -1.7 2,715,700 + Pf6N2E2_2294 0.30 -0.7 2,715,700 + Pf6N2E2_2294 0.30 -1.7 2,715,701 - Pf6N2E2_2294 0.30 -1.7 2,716,018 + Pf6N2E2_2294 0.47 -1.2 2,716,019 - Pf6N2E2_2294 0.47 -2.6 2,716,019 - Pf6N2E2_2294 0.47 -0.2 2,716,167 + Pf6N2E2_2294 0.54 -1.9 2,716,213 + Pf6N2E2_2294 0.57 +0.1 2,716,214 - Pf6N2E2_2294 0.57 +0.1 2,716,214 - Pf6N2E2_2294 0.57 -2.6 2,716,214 - Pf6N2E2_2294 0.57 +0.1 2,716,214 - Pf6N2E2_2294 0.57 -3.0 2,716,214 - Pf6N2E2_2294 0.57 -0.6 2,716,243 + Pf6N2E2_2294 0.58 +0.5 2,716,279 + Pf6N2E2_2294 0.60 -2.1 2,716,408 + Pf6N2E2_2294 0.67 -0.4 2,716,409 - Pf6N2E2_2294 0.67 -2.2 2,716,466 - Pf6N2E2_2294 0.70 -0.2 2,716,466 - Pf6N2E2_2294 0.70 -0.7 2,716,480 + Pf6N2E2_2294 0.70 -0.6 2,716,480 + Pf6N2E2_2294 0.70 -1.3 2,716,481 - Pf6N2E2_2294 0.71 -0.7 2,716,516 + Pf6N2E2_2294 0.72 -0.2 2,716,516 + Pf6N2E2_2294 0.72 -0.3 2,716,517 - Pf6N2E2_2294 0.72 -1.5 2,716,517 - Pf6N2E2_2294 0.72 -1.2 2,716,517 - Pf6N2E2_2294 0.72 -2.2 2,716,517 - Pf6N2E2_2294 0.72 -1.5 2,716,517 - Pf6N2E2_2294 0.72 +0.1 2,716,949 - -0.8 2,716,949 - +0.2 2,716,949 - +0.0 2,716,949 - -2.3 2,716,970 - -1.1 2,716,981 + -0.8 2,716,981 + -0.6 2,716,982 - -0.1 2,716,982 - -1.2 2,716,982 - -0.4 2,716,982 - -0.4 2,717,032 + +0.6 2,717,032 + +1.3 2,717,032 + -1.1 2,717,122 + -0.1 2,717,223 + +0.5 2,717,223 + +0.1 2,717,224 - -0.5 2,717,626 + Pf6N2E2_2295 0.26 +0.0 2,717,627 - Pf6N2E2_2295 0.26 +1.0 2,717,627 - Pf6N2E2_2295 0.26 -0.1 2,717,627 - Pf6N2E2_2295 0.26 -0.3 2,717,627 - Pf6N2E2_2295 0.26 -0.1 2,717,627 - Pf6N2E2_2295 0.26 +0.5 2,717,757 + Pf6N2E2_2295 0.33 +0.2 2,717,757 + Pf6N2E2_2295 0.33 +0.7 2,717,758 - Pf6N2E2_2295 0.33 +0.2 2,717,758 - Pf6N2E2_2295 0.33 +1.2 2,717,758 - Pf6N2E2_2295 0.33 +0.2 2,717,758 - Pf6N2E2_2295 0.33 +0.6 2,717,758 - Pf6N2E2_2295 0.33 -0.5 2,717,758 - Pf6N2E2_2295 0.33 +0.5 2,717,758 - Pf6N2E2_2295 0.33 +1.0 2,717,758 - Pf6N2E2_2295 0.33 +1.1 2,717,778 + Pf6N2E2_2295 0.34 +0.1 2,717,778 + Pf6N2E2_2295 0.34 +0.2 2,717,778 + Pf6N2E2_2295 0.34 +0.5 2,717,778 + Pf6N2E2_2295 0.34 +2.2 2,717,778 + Pf6N2E2_2295 0.34 +1.5 2,717,778 + Pf6N2E2_2295 0.34 +1.0 2,717,779 - Pf6N2E2_2295 0.34 -1.3 2,717,779 - Pf6N2E2_2295 0.34 +0.9 2,717,779 - Pf6N2E2_2295 0.34 +0.3 2,717,865 + Pf6N2E2_2295 0.39 +1.6 2,717,865 + Pf6N2E2_2295 0.39 +0.4 2,717,866 - Pf6N2E2_2295 0.39 -0.3 2,717,988 - Pf6N2E2_2295 0.46 -0.1
Or see this region's nucleotide sequence