Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2127 and Pf6N2E2_2128 are separated by 109 nucleotides Pf6N2E2_2128 and Pf6N2E2_2129 are separated by 79 nucleotides Pf6N2E2_2129 and Pf6N2E2_2130 are separated by 228 nucleotides
Pf6N2E2_2127 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 2,531,285 to 2,532,694
_2127
Pf6N2E2_2128 - Short-chain dehydrogenase/reductase SDR, at 2,532,804 to 2,533,550
_2128
Pf6N2E2_2129 - Predicted dye-decolorizing peroxidase (DyP), YfeX-like subgroup, at 2,533,630 to 2,534,508
_2129
Pf6N2E2_2130 - Beta-lactamase (EC 3.5.2.6), at 2,534,737 to 2,535,885
_2130
Position (kb)
2533
2534
2535 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2532.790 kb on + strand at 2532.790 kb on + strand at 2532.791 kb on - strand at 2532.791 kb on - strand at 2532.845 kb on - strand at 2532.979 kb on + strand, within Pf6N2E2_2128 at 2532.979 kb on + strand, within Pf6N2E2_2128 at 2532.979 kb on + strand, within Pf6N2E2_2128 at 2533.090 kb on + strand, within Pf6N2E2_2128 at 2533.090 kb on + strand, within Pf6N2E2_2128 at 2533.090 kb on + strand, within Pf6N2E2_2128 at 2533.091 kb on - strand, within Pf6N2E2_2128 at 2533.212 kb on + strand, within Pf6N2E2_2128 at 2533.212 kb on + strand, within Pf6N2E2_2128 at 2533.213 kb on - strand, within Pf6N2E2_2128 at 2533.453 kb on + strand, within Pf6N2E2_2128 at 2533.454 kb on - strand, within Pf6N2E2_2128 at 2533.454 kb on - strand, within Pf6N2E2_2128 at 2533.545 kb on - strand at 2533.594 kb on + strand at 2533.595 kb on - strand at 2533.685 kb on + strand at 2533.685 kb on + strand at 2533.685 kb on + strand at 2533.686 kb on - strand at 2534.143 kb on - strand, within Pf6N2E2_2129 at 2534.143 kb on - strand, within Pf6N2E2_2129 at 2534.143 kb on - strand, within Pf6N2E2_2129 at 2534.167 kb on + strand, within Pf6N2E2_2129 at 2534.407 kb on + strand, within Pf6N2E2_2129 at 2534.499 kb on - strand at 2534.499 kb on - strand at 2534.501 kb on + strand at 2534.501 kb on + strand at 2534.501 kb on + strand at 2534.502 kb on - strand at 2535.020 kb on - strand, within Pf6N2E2_2130 at 2535.127 kb on + strand, within Pf6N2E2_2130 at 2535.127 kb on + strand, within Pf6N2E2_2130 at 2535.127 kb on + strand, within Pf6N2E2_2130 at 2535.127 kb on + strand, within Pf6N2E2_2130 at 2535.127 kb on + strand, within Pf6N2E2_2130 at 2535.127 kb on + strand, within Pf6N2E2_2130 at 2535.128 kb on - strand, within Pf6N2E2_2130 at 2535.128 kb on - strand, within Pf6N2E2_2130 at 2535.128 kb on - strand, within Pf6N2E2_2130 at 2535.128 kb on - strand, within Pf6N2E2_2130 at 2535.193 kb on + strand, within Pf6N2E2_2130 at 2535.196 kb on + strand, within Pf6N2E2_2130 at 2535.196 kb on + strand, within Pf6N2E2_2130 at 2535.196 kb on + strand, within Pf6N2E2_2130 at 2535.197 kb on - strand, within Pf6N2E2_2130 at 2535.197 kb on - strand, within Pf6N2E2_2130 at 2535.197 kb on - strand, within Pf6N2E2_2130 at 2535.241 kb on + strand, within Pf6N2E2_2130 at 2535.241 kb on + strand, within Pf6N2E2_2130 at 2535.355 kb on + strand, within Pf6N2E2_2130 at 2535.355 kb on + strand, within Pf6N2E2_2130 at 2535.355 kb on + strand, within Pf6N2E2_2130 at 2535.355 kb on + strand, within Pf6N2E2_2130 at 2535.355 kb on + strand, within Pf6N2E2_2130 at 2535.355 kb on + strand, within Pf6N2E2_2130 at 2535.355 kb on + strand, within Pf6N2E2_2130 at 2535.355 kb on + strand, within Pf6N2E2_2130 at 2535.356 kb on - strand, within Pf6N2E2_2130 at 2535.356 kb on - strand, within Pf6N2E2_2130 at 2535.356 kb on - strand, within Pf6N2E2_2130 at 2535.356 kb on - strand, within Pf6N2E2_2130 at 2535.356 kb on - strand, within Pf6N2E2_2130 at 2535.356 kb on - strand, within Pf6N2E2_2130 at 2535.356 kb on - strand, within Pf6N2E2_2130 at 2535.356 kb on - strand, within Pf6N2E2_2130 at 2535.356 kb on - strand, within Pf6N2E2_2130 at 2535.356 kb on - strand, within Pf6N2E2_2130 at 2535.356 kb on - strand, within Pf6N2E2_2130 at 2535.356 kb on - strand, within Pf6N2E2_2130 at 2535.457 kb on + strand, within Pf6N2E2_2130 at 2535.457 kb on + strand, within Pf6N2E2_2130 at 2535.458 kb on - strand, within Pf6N2E2_2130 at 2535.458 kb on - strand, within Pf6N2E2_2130
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 2,532,790 + +0.5 2,532,790 + -1.5 2,532,791 - -0.6 2,532,791 - -1.2 2,532,845 - -1.3 2,532,979 + Pf6N2E2_2128 0.23 -1.0 2,532,979 + Pf6N2E2_2128 0.23 -1.5 2,532,979 + Pf6N2E2_2128 0.23 -0.7 2,533,090 + Pf6N2E2_2128 0.38 +1.0 2,533,090 + Pf6N2E2_2128 0.38 -0.6 2,533,090 + Pf6N2E2_2128 0.38 +0.5 2,533,091 - Pf6N2E2_2128 0.38 -2.0 2,533,212 + Pf6N2E2_2128 0.55 -0.5 2,533,212 + Pf6N2E2_2128 0.55 -1.1 2,533,213 - Pf6N2E2_2128 0.55 -0.2 2,533,453 + Pf6N2E2_2128 0.87 -0.6 2,533,454 - Pf6N2E2_2128 0.87 -2.2 2,533,454 - Pf6N2E2_2128 0.87 -1.6 2,533,545 - -2.2 2,533,594 + -1.6 2,533,595 - -2.0 2,533,685 + -2.6 2,533,685 + -4.1 2,533,685 + -2.1 2,533,686 - -2.1 2,534,143 - Pf6N2E2_2129 0.58 -2.6 2,534,143 - Pf6N2E2_2129 0.58 -3.5 2,534,143 - Pf6N2E2_2129 0.58 -4.8 2,534,167 + Pf6N2E2_2129 0.61 -4.1 2,534,407 + Pf6N2E2_2129 0.88 -2.8 2,534,499 - -2.8 2,534,499 - -3.8 2,534,501 + -2.4 2,534,501 + -3.2 2,534,501 + -1.6 2,534,502 - -2.8 2,535,020 - Pf6N2E2_2130 0.25 +0.6 2,535,127 + Pf6N2E2_2130 0.34 -0.2 2,535,127 + Pf6N2E2_2130 0.34 -0.8 2,535,127 + Pf6N2E2_2130 0.34 +0.8 2,535,127 + Pf6N2E2_2130 0.34 +1.1 2,535,127 + Pf6N2E2_2130 0.34 -0.3 2,535,127 + Pf6N2E2_2130 0.34 -0.3 2,535,128 - Pf6N2E2_2130 0.34 -0.2 2,535,128 - Pf6N2E2_2130 0.34 +0.3 2,535,128 - Pf6N2E2_2130 0.34 -1.5 2,535,128 - Pf6N2E2_2130 0.34 +0.3 2,535,193 + Pf6N2E2_2130 0.40 -1.0 2,535,196 + Pf6N2E2_2130 0.40 -0.3 2,535,196 + Pf6N2E2_2130 0.40 -0.5 2,535,196 + Pf6N2E2_2130 0.40 -0.5 2,535,197 - Pf6N2E2_2130 0.40 +0.3 2,535,197 - Pf6N2E2_2130 0.40 +0.7 2,535,197 - Pf6N2E2_2130 0.40 +0.7 2,535,241 + Pf6N2E2_2130 0.44 -1.0 2,535,241 + Pf6N2E2_2130 0.44 +0.2 2,535,355 + Pf6N2E2_2130 0.54 -0.2 2,535,355 + Pf6N2E2_2130 0.54 -1.7 2,535,355 + Pf6N2E2_2130 0.54 +1.5 2,535,355 + Pf6N2E2_2130 0.54 -0.5 2,535,355 + Pf6N2E2_2130 0.54 +0.2 2,535,355 + Pf6N2E2_2130 0.54 -1.0 2,535,355 + Pf6N2E2_2130 0.54 -0.4 2,535,355 + Pf6N2E2_2130 0.54 +0.6 2,535,356 - Pf6N2E2_2130 0.54 -0.8 2,535,356 - Pf6N2E2_2130 0.54 +0.4 2,535,356 - Pf6N2E2_2130 0.54 +0.2 2,535,356 - Pf6N2E2_2130 0.54 -2.7 2,535,356 - Pf6N2E2_2130 0.54 -0.3 2,535,356 - Pf6N2E2_2130 0.54 -0.4 2,535,356 - Pf6N2E2_2130 0.54 +0.8 2,535,356 - Pf6N2E2_2130 0.54 +0.6 2,535,356 - Pf6N2E2_2130 0.54 +0.6 2,535,356 - Pf6N2E2_2130 0.54 +0.1 2,535,356 - Pf6N2E2_2130 0.54 +0.4 2,535,356 - Pf6N2E2_2130 0.54 +1.8 2,535,457 + Pf6N2E2_2130 0.63 -0.6 2,535,457 + Pf6N2E2_2130 0.63 -0.5 2,535,458 - Pf6N2E2_2130 0.63 +0.5 2,535,458 - Pf6N2E2_2130 0.63 +0.2
Or see this region's nucleotide sequence