Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2033

Experiment: Choline Lysine 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2032 and Pf6N2E2_2033 are separated by 8 nucleotidesPf6N2E2_2033 and Pf6N2E2_2034 are separated by 5 nucleotidesPf6N2E2_2034 and Pf6N2E2_2035 are separated by 67 nucleotides Pf6N2E2_2032 - Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1), at 2,420,802 to 2,421,824 _2032 Pf6N2E2_2033 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 2,421,833 to 2,423,425 _2033 Pf6N2E2_2034 - Methyltransferase, at 2,423,431 to 2,424,294 _2034 Pf6N2E2_2035 - epoxide hydrolase, at 2,424,362 to 2,425,366 _2035 Position (kb) 2421 2422 2423 2424Strain fitness (log2 ratio) -3 -2 -1 0 1at 2420.952 kb on + strand, within Pf6N2E2_2032at 2420.953 kb on - strand, within Pf6N2E2_2032at 2420.968 kb on - strand, within Pf6N2E2_2032at 2421.045 kb on + strand, within Pf6N2E2_2032at 2421.045 kb on + strand, within Pf6N2E2_2032at 2421.046 kb on - strand, within Pf6N2E2_2032at 2421.046 kb on - strand, within Pf6N2E2_2032at 2421.046 kb on - strand, within Pf6N2E2_2032at 2421.046 kb on - strand, within Pf6N2E2_2032at 2421.046 kb on - strand, within Pf6N2E2_2032at 2421.113 kb on + strand, within Pf6N2E2_2032at 2421.113 kb on + strand, within Pf6N2E2_2032at 2421.114 kb on - strand, within Pf6N2E2_2032at 2421.123 kb on + strand, within Pf6N2E2_2032at 2421.123 kb on + strand, within Pf6N2E2_2032at 2421.123 kb on + strand, within Pf6N2E2_2032at 2421.124 kb on - strand, within Pf6N2E2_2032at 2421.124 kb on - strand, within Pf6N2E2_2032at 2421.124 kb on - strand, within Pf6N2E2_2032at 2421.124 kb on - strand, within Pf6N2E2_2032at 2421.233 kb on + strand, within Pf6N2E2_2032at 2421.303 kb on + strand, within Pf6N2E2_2032at 2421.304 kb on - strand, within Pf6N2E2_2032at 2421.306 kb on + strand, within Pf6N2E2_2032at 2421.307 kb on - strand, within Pf6N2E2_2032at 2421.307 kb on - strand, within Pf6N2E2_2032at 2421.372 kb on + strand, within Pf6N2E2_2032at 2421.372 kb on + strand, within Pf6N2E2_2032at 2421.373 kb on - strand, within Pf6N2E2_2032at 2421.504 kb on + strand, within Pf6N2E2_2032at 2421.504 kb on + strand, within Pf6N2E2_2032at 2421.504 kb on + strand, within Pf6N2E2_2032at 2421.504 kb on + strand, within Pf6N2E2_2032at 2421.505 kb on - strand, within Pf6N2E2_2032at 2421.594 kb on + strand, within Pf6N2E2_2032at 2421.594 kb on + strand, within Pf6N2E2_2032at 2421.594 kb on + strand, within Pf6N2E2_2032at 2421.595 kb on - strand, within Pf6N2E2_2032at 2421.865 kb on - strandat 2422.589 kb on + strand, within Pf6N2E2_2033at 2422.589 kb on + strand, within Pf6N2E2_2033at 2422.590 kb on - strand, within Pf6N2E2_2033at 2422.721 kb on + strand, within Pf6N2E2_2033at 2422.722 kb on - strand, within Pf6N2E2_2033at 2422.741 kb on + strand, within Pf6N2E2_2033at 2422.742 kb on - strand, within Pf6N2E2_2033at 2423.249 kb on + strand, within Pf6N2E2_2033at 2423.261 kb on + strand, within Pf6N2E2_2033at 2423.261 kb on + strand, within Pf6N2E2_2033at 2423.262 kb on - strand, within Pf6N2E2_2033at 2423.262 kb on - strand, within Pf6N2E2_2033at 2423.387 kb on + strandat 2423.387 kb on + strandat 2423.387 kb on + strandat 2423.388 kb on - strandat 2423.388 kb on - strandat 2423.389 kb on + strandat 2423.390 kb on - strandat 2423.567 kb on - strand, within Pf6N2E2_2034at 2423.567 kb on - strand, within Pf6N2E2_2034at 2423.889 kb on + strand, within Pf6N2E2_2034at 2424.013 kb on - strand, within Pf6N2E2_2034at 2424.275 kb on + strandat 2424.364 kb on - strandat 2424.364 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline Lysine 20 mM
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2,420,952 + Pf6N2E2_2032 0.15 -1.3
2,420,953 - Pf6N2E2_2032 0.15 -0.0
2,420,968 - Pf6N2E2_2032 0.16 -1.6
2,421,045 + Pf6N2E2_2032 0.24 +0.2
2,421,045 + Pf6N2E2_2032 0.24 -0.0
2,421,046 - Pf6N2E2_2032 0.24 -0.2
2,421,046 - Pf6N2E2_2032 0.24 -0.1
2,421,046 - Pf6N2E2_2032 0.24 -0.9
2,421,046 - Pf6N2E2_2032 0.24 -1.1
2,421,046 - Pf6N2E2_2032 0.24 -0.5
2,421,113 + Pf6N2E2_2032 0.30 -2.0
2,421,113 + Pf6N2E2_2032 0.30 +0.3
2,421,114 - Pf6N2E2_2032 0.30 -0.9
2,421,123 + Pf6N2E2_2032 0.31 +0.3
2,421,123 + Pf6N2E2_2032 0.31 +0.6
2,421,123 + Pf6N2E2_2032 0.31 -1.8
2,421,124 - Pf6N2E2_2032 0.31 -0.7
2,421,124 - Pf6N2E2_2032 0.31 -0.5
2,421,124 - Pf6N2E2_2032 0.31 -2.9
2,421,124 - Pf6N2E2_2032 0.31 -2.9
2,421,233 + Pf6N2E2_2032 0.42 -1.1
2,421,303 + Pf6N2E2_2032 0.49 -1.0
2,421,304 - Pf6N2E2_2032 0.49 +0.2
2,421,306 + Pf6N2E2_2032 0.49 -0.2
2,421,307 - Pf6N2E2_2032 0.49 -0.7
2,421,307 - Pf6N2E2_2032 0.49 -0.9
2,421,372 + Pf6N2E2_2032 0.56 -0.2
2,421,372 + Pf6N2E2_2032 0.56 -1.3
2,421,373 - Pf6N2E2_2032 0.56 -0.5
2,421,504 + Pf6N2E2_2032 0.69 +0.1
2,421,504 + Pf6N2E2_2032 0.69 -0.5
2,421,504 + Pf6N2E2_2032 0.69 -2.0
2,421,504 + Pf6N2E2_2032 0.69 +0.6
2,421,505 - Pf6N2E2_2032 0.69 -0.1
2,421,594 + Pf6N2E2_2032 0.77 -0.6
2,421,594 + Pf6N2E2_2032 0.77 -0.5
2,421,594 + Pf6N2E2_2032 0.77 +0.1
2,421,595 - Pf6N2E2_2032 0.78 -0.6
2,421,865 - -0.5
2,422,589 + Pf6N2E2_2033 0.47 -0.3
2,422,589 + Pf6N2E2_2033 0.47 -1.1
2,422,590 - Pf6N2E2_2033 0.48 -0.5
2,422,721 + Pf6N2E2_2033 0.56 -2.2
2,422,722 - Pf6N2E2_2033 0.56 +0.1
2,422,741 + Pf6N2E2_2033 0.57 -1.1
2,422,742 - Pf6N2E2_2033 0.57 -0.3
2,423,249 + Pf6N2E2_2033 0.89 -2.4
2,423,261 + Pf6N2E2_2033 0.90 -1.5
2,423,261 + Pf6N2E2_2033 0.90 -0.7
2,423,262 - Pf6N2E2_2033 0.90 -0.9
2,423,262 - Pf6N2E2_2033 0.90 -1.2
2,423,387 + -0.4
2,423,387 + -1.1
2,423,387 + -0.3
2,423,388 - -0.3
2,423,388 - -1.4
2,423,389 + -1.3
2,423,390 - -0.7
2,423,567 - Pf6N2E2_2034 0.16 -2.0
2,423,567 - Pf6N2E2_2034 0.16 -0.3
2,423,889 + Pf6N2E2_2034 0.53 +0.0
2,424,013 - Pf6N2E2_2034 0.67 +0.2
2,424,275 + +0.3
2,424,364 - -1.5
2,424,364 - -2.0

Or see this region's nucleotide sequence