Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2032 and Pf6N2E2_2033 are separated by 8 nucleotides Pf6N2E2_2033 and Pf6N2E2_2034 are separated by 5 nucleotides Pf6N2E2_2034 and Pf6N2E2_2035 are separated by 67 nucleotides
Pf6N2E2_2032 - Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1), at 2,420,802 to 2,421,824
_2032
Pf6N2E2_2033 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 2,421,833 to 2,423,425
_2033
Pf6N2E2_2034 - Methyltransferase, at 2,423,431 to 2,424,294
_2034
Pf6N2E2_2035 - epoxide hydrolase, at 2,424,362 to 2,425,366
_2035
Position (kb)
2421
2422
2423
2424 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2420.952 kb on + strand, within Pf6N2E2_2032 at 2420.953 kb on - strand, within Pf6N2E2_2032 at 2420.968 kb on - strand, within Pf6N2E2_2032 at 2421.045 kb on + strand, within Pf6N2E2_2032 at 2421.045 kb on + strand, within Pf6N2E2_2032 at 2421.046 kb on - strand, within Pf6N2E2_2032 at 2421.046 kb on - strand, within Pf6N2E2_2032 at 2421.046 kb on - strand, within Pf6N2E2_2032 at 2421.046 kb on - strand, within Pf6N2E2_2032 at 2421.046 kb on - strand, within Pf6N2E2_2032 at 2421.113 kb on + strand, within Pf6N2E2_2032 at 2421.113 kb on + strand, within Pf6N2E2_2032 at 2421.114 kb on - strand, within Pf6N2E2_2032 at 2421.123 kb on + strand, within Pf6N2E2_2032 at 2421.123 kb on + strand, within Pf6N2E2_2032 at 2421.123 kb on + strand, within Pf6N2E2_2032 at 2421.124 kb on - strand, within Pf6N2E2_2032 at 2421.124 kb on - strand, within Pf6N2E2_2032 at 2421.124 kb on - strand, within Pf6N2E2_2032 at 2421.124 kb on - strand, within Pf6N2E2_2032 at 2421.233 kb on + strand, within Pf6N2E2_2032 at 2421.303 kb on + strand, within Pf6N2E2_2032 at 2421.304 kb on - strand, within Pf6N2E2_2032 at 2421.306 kb on + strand, within Pf6N2E2_2032 at 2421.307 kb on - strand, within Pf6N2E2_2032 at 2421.307 kb on - strand, within Pf6N2E2_2032 at 2421.372 kb on + strand, within Pf6N2E2_2032 at 2421.372 kb on + strand, within Pf6N2E2_2032 at 2421.373 kb on - strand, within Pf6N2E2_2032 at 2421.504 kb on + strand, within Pf6N2E2_2032 at 2421.504 kb on + strand, within Pf6N2E2_2032 at 2421.504 kb on + strand, within Pf6N2E2_2032 at 2421.504 kb on + strand, within Pf6N2E2_2032 at 2421.505 kb on - strand, within Pf6N2E2_2032 at 2421.594 kb on + strand, within Pf6N2E2_2032 at 2421.594 kb on + strand, within Pf6N2E2_2032 at 2421.594 kb on + strand, within Pf6N2E2_2032 at 2421.595 kb on - strand, within Pf6N2E2_2032 at 2421.865 kb on - strand at 2422.589 kb on + strand, within Pf6N2E2_2033 at 2422.589 kb on + strand, within Pf6N2E2_2033 at 2422.590 kb on - strand, within Pf6N2E2_2033 at 2422.721 kb on + strand, within Pf6N2E2_2033 at 2422.722 kb on - strand, within Pf6N2E2_2033 at 2422.741 kb on + strand, within Pf6N2E2_2033 at 2422.742 kb on - strand, within Pf6N2E2_2033 at 2423.249 kb on + strand, within Pf6N2E2_2033 at 2423.261 kb on + strand, within Pf6N2E2_2033 at 2423.261 kb on + strand, within Pf6N2E2_2033 at 2423.262 kb on - strand, within Pf6N2E2_2033 at 2423.262 kb on - strand, within Pf6N2E2_2033 at 2423.387 kb on + strand at 2423.387 kb on + strand at 2423.387 kb on + strand at 2423.388 kb on - strand at 2423.388 kb on - strand at 2423.389 kb on + strand at 2423.390 kb on - strand at 2423.567 kb on - strand, within Pf6N2E2_2034 at 2423.567 kb on - strand, within Pf6N2E2_2034 at 2423.889 kb on + strand, within Pf6N2E2_2034 at 2424.013 kb on - strand, within Pf6N2E2_2034 at 2424.275 kb on + strand at 2424.364 kb on - strand at 2424.364 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 2,420,952 + Pf6N2E2_2032 0.15 -1.3 2,420,953 - Pf6N2E2_2032 0.15 -0.0 2,420,968 - Pf6N2E2_2032 0.16 -1.6 2,421,045 + Pf6N2E2_2032 0.24 +0.2 2,421,045 + Pf6N2E2_2032 0.24 -0.0 2,421,046 - Pf6N2E2_2032 0.24 -0.2 2,421,046 - Pf6N2E2_2032 0.24 -0.1 2,421,046 - Pf6N2E2_2032 0.24 -0.9 2,421,046 - Pf6N2E2_2032 0.24 -1.1 2,421,046 - Pf6N2E2_2032 0.24 -0.5 2,421,113 + Pf6N2E2_2032 0.30 -2.0 2,421,113 + Pf6N2E2_2032 0.30 +0.3 2,421,114 - Pf6N2E2_2032 0.30 -0.9 2,421,123 + Pf6N2E2_2032 0.31 +0.3 2,421,123 + Pf6N2E2_2032 0.31 +0.6 2,421,123 + Pf6N2E2_2032 0.31 -1.8 2,421,124 - Pf6N2E2_2032 0.31 -0.7 2,421,124 - Pf6N2E2_2032 0.31 -0.5 2,421,124 - Pf6N2E2_2032 0.31 -2.9 2,421,124 - Pf6N2E2_2032 0.31 -2.9 2,421,233 + Pf6N2E2_2032 0.42 -1.1 2,421,303 + Pf6N2E2_2032 0.49 -1.0 2,421,304 - Pf6N2E2_2032 0.49 +0.2 2,421,306 + Pf6N2E2_2032 0.49 -0.2 2,421,307 - Pf6N2E2_2032 0.49 -0.7 2,421,307 - Pf6N2E2_2032 0.49 -0.9 2,421,372 + Pf6N2E2_2032 0.56 -0.2 2,421,372 + Pf6N2E2_2032 0.56 -1.3 2,421,373 - Pf6N2E2_2032 0.56 -0.5 2,421,504 + Pf6N2E2_2032 0.69 +0.1 2,421,504 + Pf6N2E2_2032 0.69 -0.5 2,421,504 + Pf6N2E2_2032 0.69 -2.0 2,421,504 + Pf6N2E2_2032 0.69 +0.6 2,421,505 - Pf6N2E2_2032 0.69 -0.1 2,421,594 + Pf6N2E2_2032 0.77 -0.6 2,421,594 + Pf6N2E2_2032 0.77 -0.5 2,421,594 + Pf6N2E2_2032 0.77 +0.1 2,421,595 - Pf6N2E2_2032 0.78 -0.6 2,421,865 - -0.5 2,422,589 + Pf6N2E2_2033 0.47 -0.3 2,422,589 + Pf6N2E2_2033 0.47 -1.1 2,422,590 - Pf6N2E2_2033 0.48 -0.5 2,422,721 + Pf6N2E2_2033 0.56 -2.2 2,422,722 - Pf6N2E2_2033 0.56 +0.1 2,422,741 + Pf6N2E2_2033 0.57 -1.1 2,422,742 - Pf6N2E2_2033 0.57 -0.3 2,423,249 + Pf6N2E2_2033 0.89 -2.4 2,423,261 + Pf6N2E2_2033 0.90 -1.5 2,423,261 + Pf6N2E2_2033 0.90 -0.7 2,423,262 - Pf6N2E2_2033 0.90 -0.9 2,423,262 - Pf6N2E2_2033 0.90 -1.2 2,423,387 + -0.4 2,423,387 + -1.1 2,423,387 + -0.3 2,423,388 - -0.3 2,423,388 - -1.4 2,423,389 + -1.3 2,423,390 - -0.7 2,423,567 - Pf6N2E2_2034 0.16 -2.0 2,423,567 - Pf6N2E2_2034 0.16 -0.3 2,423,889 + Pf6N2E2_2034 0.53 +0.0 2,424,013 - Pf6N2E2_2034 0.67 +0.2 2,424,275 + +0.3 2,424,364 - -1.5 2,424,364 - -2.0
Or see this region's nucleotide sequence