Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2016 and Pf6N2E2_2017 are separated by 164 nucleotides Pf6N2E2_2017 and Pf6N2E2_2018 are separated by 1 nucleotides Pf6N2E2_2018 and Pf6N2E2_2019 are separated by 26 nucleotides
Pf6N2E2_2016 - hypothetical protein, at 2,401,994 to 2,404,036
_2016
Pf6N2E2_2017 - DNA-binding protein, at 2,404,201 to 2,404,482
_2017
Pf6N2E2_2018 - hypothetical protein, at 2,404,484 to 2,404,630
_2018
Pf6N2E2_2019 - hypothetical protein, at 2,404,657 to 2,408,535
_2019
Position (kb)
2404
2405 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2403.545 kb on + strand, within Pf6N2E2_2016 at 2403.545 kb on + strand, within Pf6N2E2_2016 at 2403.546 kb on - strand, within Pf6N2E2_2016 at 2403.546 kb on - strand, within Pf6N2E2_2016 at 2403.675 kb on - strand, within Pf6N2E2_2016 at 2403.675 kb on - strand, within Pf6N2E2_2016 at 2403.703 kb on + strand, within Pf6N2E2_2016 at 2403.710 kb on + strand, within Pf6N2E2_2016 at 2403.710 kb on + strand, within Pf6N2E2_2016 at 2403.710 kb on + strand, within Pf6N2E2_2016 at 2403.711 kb on - strand, within Pf6N2E2_2016 at 2403.711 kb on - strand, within Pf6N2E2_2016 at 2403.711 kb on - strand, within Pf6N2E2_2016 at 2403.711 kb on - strand, within Pf6N2E2_2016 at 2403.711 kb on - strand, within Pf6N2E2_2016 at 2403.711 kb on - strand, within Pf6N2E2_2016 at 2403.724 kb on + strand, within Pf6N2E2_2016 at 2403.730 kb on + strand, within Pf6N2E2_2016 at 2403.730 kb on + strand, within Pf6N2E2_2016 at 2403.730 kb on + strand, within Pf6N2E2_2016 at 2403.731 kb on - strand, within Pf6N2E2_2016 at 2403.731 kb on - strand, within Pf6N2E2_2016 at 2403.731 kb on - strand, within Pf6N2E2_2016 at 2403.731 kb on - strand, within Pf6N2E2_2016 at 2403.731 kb on - strand, within Pf6N2E2_2016 at 2403.731 kb on - strand, within Pf6N2E2_2016 at 2403.812 kb on - strand, within Pf6N2E2_2016 at 2404.014 kb on + strand at 2404.014 kb on + strand at 2404.014 kb on + strand at 2404.014 kb on + strand at 2404.014 kb on + strand at 2404.014 kb on + strand at 2404.014 kb on + strand at 2404.014 kb on + strand at 2404.015 kb on - strand at 2404.015 kb on - strand at 2404.015 kb on - strand at 2404.015 kb on - strand at 2404.015 kb on - strand at 2404.015 kb on - strand at 2404.041 kb on + strand at 2404.045 kb on + strand at 2404.045 kb on + strand at 2404.046 kb on - strand at 2404.046 kb on - strand at 2404.046 kb on - strand at 2404.064 kb on + strand at 2404.065 kb on - strand at 2404.140 kb on + strand at 2404.140 kb on + strand at 2404.140 kb on + strand at 2404.141 kb on - strand at 2404.141 kb on - strand at 2404.141 kb on - strand at 2404.141 kb on - strand at 2404.141 kb on - strand at 2404.141 kb on - strand at 2404.144 kb on + strand at 2404.144 kb on + strand at 2404.145 kb on - strand at 2404.145 kb on - strand at 2404.271 kb on + strand, within Pf6N2E2_2017 at 2404.449 kb on + strand, within Pf6N2E2_2017 at 2404.450 kb on - strand, within Pf6N2E2_2017 at 2404.474 kb on + strand at 2404.485 kb on + strand at 2404.485 kb on + strand at 2404.486 kb on - strand at 2404.486 kb on - strand at 2404.570 kb on + strand, within Pf6N2E2_2018 at 2404.571 kb on - strand, within Pf6N2E2_2018 at 2404.630 kb on + strand at 2404.631 kb on - strand at 2404.631 kb on - strand at 2404.654 kb on + strand at 2404.654 kb on + strand at 2404.654 kb on + strand at 2404.654 kb on + strand at 2404.654 kb on + strand at 2404.654 kb on + strand at 2404.655 kb on - strand at 2404.665 kb on + strand at 2404.665 kb on + strand at 2404.665 kb on + strand at 2404.665 kb on + strand at 2404.666 kb on - strand at 2404.695 kb on - strand at 2404.695 kb on - strand at 2404.695 kb on - strand at 2404.701 kb on + strand at 2404.702 kb on - strand at 2404.702 kb on - strand at 2404.732 kb on - strand at 2404.770 kb on + strand at 2404.770 kb on + strand at 2404.770 kb on + strand at 2404.861 kb on + strand at 2404.861 kb on + strand at 2404.861 kb on + strand at 2404.862 kb on - strand at 2404.862 kb on - strand at 2404.862 kb on - strand at 2404.867 kb on + strand at 2404.867 kb on + strand at 2404.868 kb on - strand at 2404.868 kb on - strand at 2404.924 kb on + strand at 2404.924 kb on + strand at 2404.925 kb on - strand at 2404.928 kb on + strand at 2404.928 kb on + strand at 2404.928 kb on + strand at 2404.929 kb on - strand at 2404.929 kb on - strand at 2404.976 kb on + strand at 2404.976 kb on + strand at 2404.977 kb on - strand at 2405.092 kb on - strand, within Pf6N2E2_2019 at 2405.108 kb on + strand, within Pf6N2E2_2019 at 2405.108 kb on + strand, within Pf6N2E2_2019 at 2405.108 kb on + strand, within Pf6N2E2_2019 at 2405.108 kb on + strand, within Pf6N2E2_2019 at 2405.109 kb on - strand, within Pf6N2E2_2019 at 2405.109 kb on - strand, within Pf6N2E2_2019 at 2405.109 kb on - strand, within Pf6N2E2_2019 at 2405.117 kb on + strand, within Pf6N2E2_2019 at 2405.118 kb on - strand, within Pf6N2E2_2019 at 2405.118 kb on - strand, within Pf6N2E2_2019 at 2405.280 kb on - strand, within Pf6N2E2_2019 at 2405.280 kb on - strand, within Pf6N2E2_2019 at 2405.282 kb on + strand, within Pf6N2E2_2019 at 2405.283 kb on - strand, within Pf6N2E2_2019 at 2405.283 kb on - strand, within Pf6N2E2_2019 at 2405.286 kb on + strand, within Pf6N2E2_2019 at 2405.287 kb on - strand, within Pf6N2E2_2019 at 2405.287 kb on - strand, within Pf6N2E2_2019 at 2405.304 kb on + strand, within Pf6N2E2_2019 at 2405.440 kb on + strand, within Pf6N2E2_2019 at 2405.441 kb on - strand, within Pf6N2E2_2019 at 2405.441 kb on - strand, within Pf6N2E2_2019 at 2405.526 kb on - strand, within Pf6N2E2_2019 at 2405.567 kb on + strand, within Pf6N2E2_2019 at 2405.571 kb on + strand, within Pf6N2E2_2019 at 2405.571 kb on + strand, within Pf6N2E2_2019 at 2405.571 kb on + strand, within Pf6N2E2_2019 at 2405.572 kb on - strand, within Pf6N2E2_2019 at 2405.572 kb on - strand, within Pf6N2E2_2019 at 2405.572 kb on - strand, within Pf6N2E2_2019 at 2405.572 kb on - strand, within Pf6N2E2_2019 at 2405.572 kb on - strand, within Pf6N2E2_2019 at 2405.572 kb on - strand, within Pf6N2E2_2019 at 2405.572 kb on - strand, within Pf6N2E2_2019 at 2405.573 kb on + strand, within Pf6N2E2_2019 at 2405.573 kb on + strand, within Pf6N2E2_2019 at 2405.573 kb on + strand, within Pf6N2E2_2019 at 2405.573 kb on + strand, within Pf6N2E2_2019 at 2405.574 kb on - strand, within Pf6N2E2_2019 at 2405.609 kb on + strand, within Pf6N2E2_2019 at 2405.609 kb on + strand, within Pf6N2E2_2019 at 2405.609 kb on + strand, within Pf6N2E2_2019 at 2405.610 kb on - strand, within Pf6N2E2_2019 at 2405.613 kb on + strand, within Pf6N2E2_2019 at 2405.613 kb on + strand, within Pf6N2E2_2019 at 2405.614 kb on - strand, within Pf6N2E2_2019
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 2,403,545 + Pf6N2E2_2016 0.76 +1.3 2,403,545 + Pf6N2E2_2016 0.76 +0.0 2,403,546 - Pf6N2E2_2016 0.76 +1.1 2,403,546 - Pf6N2E2_2016 0.76 +0.0 2,403,675 - Pf6N2E2_2016 0.82 -1.7 2,403,675 - Pf6N2E2_2016 0.82 -0.5 2,403,703 + Pf6N2E2_2016 0.84 -0.7 2,403,710 + Pf6N2E2_2016 0.84 -1.7 2,403,710 + Pf6N2E2_2016 0.84 +1.1 2,403,710 + Pf6N2E2_2016 0.84 -1.8 2,403,711 - Pf6N2E2_2016 0.84 -0.8 2,403,711 - Pf6N2E2_2016 0.84 +0.4 2,403,711 - Pf6N2E2_2016 0.84 -0.0 2,403,711 - Pf6N2E2_2016 0.84 +0.5 2,403,711 - Pf6N2E2_2016 0.84 -0.1 2,403,711 - Pf6N2E2_2016 0.84 -0.1 2,403,724 + Pf6N2E2_2016 0.85 +0.5 2,403,730 + Pf6N2E2_2016 0.85 +1.0 2,403,730 + Pf6N2E2_2016 0.85 +0.0 2,403,730 + Pf6N2E2_2016 0.85 +0.6 2,403,731 - Pf6N2E2_2016 0.85 +0.3 2,403,731 - Pf6N2E2_2016 0.85 +0.2 2,403,731 - Pf6N2E2_2016 0.85 +1.0 2,403,731 - Pf6N2E2_2016 0.85 +0.1 2,403,731 - Pf6N2E2_2016 0.85 +0.3 2,403,731 - Pf6N2E2_2016 0.85 +0.2 2,403,812 - Pf6N2E2_2016 0.89 -1.3 2,404,014 + +1.1 2,404,014 + +0.0 2,404,014 + +0.3 2,404,014 + +0.0 2,404,014 + +0.1 2,404,014 + -0.5 2,404,014 + +1.0 2,404,014 + -0.6 2,404,015 - +0.7 2,404,015 - +0.9 2,404,015 - +1.2 2,404,015 - +0.3 2,404,015 - -1.2 2,404,015 - +0.8 2,404,041 + -1.5 2,404,045 + -0.6 2,404,045 + -0.4 2,404,046 - +1.3 2,404,046 - -0.6 2,404,046 - -0.1 2,404,064 + +0.3 2,404,065 - +0.7 2,404,140 + +1.3 2,404,140 + +0.9 2,404,140 + +0.3 2,404,141 - +0.9 2,404,141 - -0.5 2,404,141 - +0.5 2,404,141 - +0.4 2,404,141 - +0.5 2,404,141 - -0.3 2,404,144 + +0.6 2,404,144 + +0.5 2,404,145 - +0.4 2,404,145 - +0.3 2,404,271 + Pf6N2E2_2017 0.25 +0.6 2,404,449 + Pf6N2E2_2017 0.88 +0.8 2,404,450 - Pf6N2E2_2017 0.88 +0.1 2,404,474 + -1.3 2,404,485 + +0.1 2,404,485 + -0.7 2,404,486 - +0.6 2,404,486 - -0.5 2,404,570 + Pf6N2E2_2018 0.59 -0.5 2,404,571 - Pf6N2E2_2018 0.59 -1.3 2,404,630 + -2.3 2,404,631 - +0.8 2,404,631 - +0.6 2,404,654 + +0.3 2,404,654 + +0.0 2,404,654 + -0.2 2,404,654 + +1.3 2,404,654 + -0.2 2,404,654 + +0.4 2,404,655 - -1.1 2,404,665 + +0.2 2,404,665 + -1.1 2,404,665 + -2.0 2,404,665 + -0.5 2,404,666 - +0.4 2,404,695 - +1.3 2,404,695 - -0.2 2,404,695 - -1.3 2,404,701 + +0.5 2,404,702 - -0.2 2,404,702 - -0.9 2,404,732 - +0.3 2,404,770 + +1.6 2,404,770 + -0.0 2,404,770 + -1.1 2,404,861 + +1.5 2,404,861 + +1.0 2,404,861 + -2.8 2,404,862 - +0.0 2,404,862 - -0.1 2,404,862 - +0.4 2,404,867 + -0.1 2,404,867 + +0.8 2,404,868 - +0.3 2,404,868 - +0.2 2,404,924 + +1.4 2,404,924 + -0.5 2,404,925 - -0.4 2,404,928 + +0.4 2,404,928 + +0.1 2,404,928 + +1.1 2,404,929 - -0.3 2,404,929 - +0.4 2,404,976 + +0.8 2,404,976 + +0.8 2,404,977 - -0.4 2,405,092 - Pf6N2E2_2019 0.11 -2.3 2,405,108 + Pf6N2E2_2019 0.12 +0.3 2,405,108 + Pf6N2E2_2019 0.12 +0.9 2,405,108 + Pf6N2E2_2019 0.12 +1.1 2,405,108 + Pf6N2E2_2019 0.12 +0.6 2,405,109 - Pf6N2E2_2019 0.12 -1.1 2,405,109 - Pf6N2E2_2019 0.12 -0.7 2,405,109 - Pf6N2E2_2019 0.12 +0.3 2,405,117 + Pf6N2E2_2019 0.12 +0.3 2,405,118 - Pf6N2E2_2019 0.12 +0.3 2,405,118 - Pf6N2E2_2019 0.12 +1.6 2,405,280 - Pf6N2E2_2019 0.16 -0.3 2,405,280 - Pf6N2E2_2019 0.16 -1.6 2,405,282 + Pf6N2E2_2019 0.16 +0.6 2,405,283 - Pf6N2E2_2019 0.16 -0.4 2,405,283 - Pf6N2E2_2019 0.16 -0.2 2,405,286 + Pf6N2E2_2019 0.16 -0.3 2,405,287 - Pf6N2E2_2019 0.16 +0.7 2,405,287 - Pf6N2E2_2019 0.16 -0.4 2,405,304 + Pf6N2E2_2019 0.17 -1.2 2,405,440 + Pf6N2E2_2019 0.20 +0.3 2,405,441 - Pf6N2E2_2019 0.20 -0.0 2,405,441 - Pf6N2E2_2019 0.20 -0.1 2,405,526 - Pf6N2E2_2019 0.22 +0.9 2,405,567 + Pf6N2E2_2019 0.23 +1.0 2,405,571 + Pf6N2E2_2019 0.24 -2.5 2,405,571 + Pf6N2E2_2019 0.24 -0.3 2,405,571 + Pf6N2E2_2019 0.24 +0.8 2,405,572 - Pf6N2E2_2019 0.24 -1.7 2,405,572 - Pf6N2E2_2019 0.24 -1.4 2,405,572 - Pf6N2E2_2019 0.24 +0.9 2,405,572 - Pf6N2E2_2019 0.24 -0.2 2,405,572 - Pf6N2E2_2019 0.24 +1.2 2,405,572 - Pf6N2E2_2019 0.24 +1.0 2,405,572 - Pf6N2E2_2019 0.24 -0.1 2,405,573 + Pf6N2E2_2019 0.24 -2.0 2,405,573 + Pf6N2E2_2019 0.24 +0.1 2,405,573 + Pf6N2E2_2019 0.24 +0.2 2,405,573 + Pf6N2E2_2019 0.24 +0.9 2,405,574 - Pf6N2E2_2019 0.24 -0.7 2,405,609 + Pf6N2E2_2019 0.25 -0.1 2,405,609 + Pf6N2E2_2019 0.25 -1.1 2,405,609 + Pf6N2E2_2019 0.25 +0.2 2,405,610 - Pf6N2E2_2019 0.25 +0.5 2,405,613 + Pf6N2E2_2019 0.25 -1.1 2,405,613 + Pf6N2E2_2019 0.25 +1.0 2,405,614 - Pf6N2E2_2019 0.25 +0.1
Or see this region's nucleotide sequence