Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1588 and Pf6N2E2_1589 overlap by 11 nucleotides Pf6N2E2_1589 and Pf6N2E2_1590 are separated by 3 nucleotides
Pf6N2E2_1588 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 1,857,902 to 1,859,422
_1588
Pf6N2E2_1589 - Phosphoserine phosphatase (EC 3.1.3.3), at 1,859,412 to 1,860,023
_1589
Pf6N2E2_1590 - pentachlorophenol 4-monooxygenase, at 1,860,027 to 1,861,790
_1590
Position (kb)
1859
1860
1861 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1858.479 kb on - strand, within Pf6N2E2_1588 at 1858.730 kb on - strand, within Pf6N2E2_1588 at 1858.788 kb on - strand, within Pf6N2E2_1588 at 1858.842 kb on + strand, within Pf6N2E2_1588 at 1858.916 kb on - strand, within Pf6N2E2_1588 at 1859.023 kb on - strand, within Pf6N2E2_1588 at 1859.023 kb on - strand, within Pf6N2E2_1588 at 1859.049 kb on - strand, within Pf6N2E2_1588 at 1859.252 kb on + strand, within Pf6N2E2_1588 at 1859.253 kb on - strand, within Pf6N2E2_1588 at 1859.351 kb on + strand at 1859.389 kb on + strand at 1859.390 kb on - strand at 1859.504 kb on + strand, within Pf6N2E2_1589 at 1859.619 kb on + strand, within Pf6N2E2_1589 at 1859.664 kb on + strand, within Pf6N2E2_1589 at 1859.665 kb on - strand, within Pf6N2E2_1589 at 1859.665 kb on - strand, within Pf6N2E2_1589 at 1859.665 kb on - strand, within Pf6N2E2_1589 at 1859.779 kb on - strand, within Pf6N2E2_1589 at 1859.779 kb on - strand, within Pf6N2E2_1589 at 1859.814 kb on + strand, within Pf6N2E2_1589 at 1859.815 kb on - strand, within Pf6N2E2_1589 at 1859.885 kb on - strand, within Pf6N2E2_1589 at 1859.946 kb on + strand, within Pf6N2E2_1589 at 1859.946 kb on + strand, within Pf6N2E2_1589 at 1859.946 kb on + strand, within Pf6N2E2_1589 at 1859.947 kb on - strand, within Pf6N2E2_1589 at 1859.947 kb on - strand, within Pf6N2E2_1589 at 1859.947 kb on - strand, within Pf6N2E2_1589 at 1860.006 kb on + strand at 1860.006 kb on + strand at 1860.007 kb on - strand at 1860.019 kb on - strand at 1860.327 kb on + strand, within Pf6N2E2_1590 at 1860.340 kb on - strand, within Pf6N2E2_1590 at 1860.606 kb on + strand, within Pf6N2E2_1590 at 1860.697 kb on - strand, within Pf6N2E2_1590 at 1860.697 kb on - strand, within Pf6N2E2_1590 at 1860.697 kb on - strand, within Pf6N2E2_1590 at 1860.697 kb on - strand, within Pf6N2E2_1590 at 1860.756 kb on + strand, within Pf6N2E2_1590 at 1860.757 kb on - strand, within Pf6N2E2_1590 at 1860.941 kb on + strand, within Pf6N2E2_1590 at 1860.993 kb on + strand, within Pf6N2E2_1590 at 1860.993 kb on + strand, within Pf6N2E2_1590 at 1860.993 kb on + strand, within Pf6N2E2_1590 at 1860.994 kb on - strand, within Pf6N2E2_1590 at 1860.996 kb on - strand, within Pf6N2E2_1590 at 1861.017 kb on + strand, within Pf6N2E2_1590 at 1861.018 kb on - strand, within Pf6N2E2_1590 at 1861.018 kb on - strand, within Pf6N2E2_1590 at 1861.018 kb on - strand, within Pf6N2E2_1590 at 1861.018 kb on - strand, within Pf6N2E2_1590 at 1861.018 kb on - strand, within Pf6N2E2_1590
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 1,858,479 - Pf6N2E2_1588 0.38 -1.2 1,858,730 - Pf6N2E2_1588 0.54 -0.4 1,858,788 - Pf6N2E2_1588 0.58 -2.0 1,858,842 + Pf6N2E2_1588 0.62 -1.3 1,858,916 - Pf6N2E2_1588 0.67 +0.5 1,859,023 - Pf6N2E2_1588 0.74 +1.3 1,859,023 - Pf6N2E2_1588 0.74 +0.1 1,859,049 - Pf6N2E2_1588 0.75 +1.5 1,859,252 + Pf6N2E2_1588 0.89 +0.4 1,859,253 - Pf6N2E2_1588 0.89 +0.9 1,859,351 + -0.2 1,859,389 + -1.0 1,859,390 - +0.9 1,859,504 + Pf6N2E2_1589 0.15 -3.0 1,859,619 + Pf6N2E2_1589 0.34 -2.7 1,859,664 + Pf6N2E2_1589 0.41 +0.4 1,859,665 - Pf6N2E2_1589 0.41 +0.3 1,859,665 - Pf6N2E2_1589 0.41 -0.7 1,859,665 - Pf6N2E2_1589 0.41 +0.5 1,859,779 - Pf6N2E2_1589 0.60 -0.1 1,859,779 - Pf6N2E2_1589 0.60 -1.0 1,859,814 + Pf6N2E2_1589 0.66 +1.2 1,859,815 - Pf6N2E2_1589 0.66 +0.7 1,859,885 - Pf6N2E2_1589 0.77 -2.1 1,859,946 + Pf6N2E2_1589 0.87 -1.2 1,859,946 + Pf6N2E2_1589 0.87 -0.1 1,859,946 + Pf6N2E2_1589 0.87 +0.0 1,859,947 - Pf6N2E2_1589 0.87 +1.4 1,859,947 - Pf6N2E2_1589 0.87 +0.0 1,859,947 - Pf6N2E2_1589 0.87 -1.1 1,860,006 + +0.5 1,860,006 + +0.2 1,860,007 - +1.8 1,860,019 - -0.9 1,860,327 + Pf6N2E2_1590 0.17 -0.3 1,860,340 - Pf6N2E2_1590 0.18 +0.3 1,860,606 + Pf6N2E2_1590 0.33 +0.9 1,860,697 - Pf6N2E2_1590 0.38 +1.6 1,860,697 - Pf6N2E2_1590 0.38 +0.4 1,860,697 - Pf6N2E2_1590 0.38 -1.1 1,860,697 - Pf6N2E2_1590 0.38 +0.3 1,860,756 + Pf6N2E2_1590 0.41 +0.3 1,860,757 - Pf6N2E2_1590 0.41 -0.2 1,860,941 + Pf6N2E2_1590 0.52 -0.2 1,860,993 + Pf6N2E2_1590 0.55 -0.6 1,860,993 + Pf6N2E2_1590 0.55 -0.1 1,860,993 + Pf6N2E2_1590 0.55 +0.4 1,860,994 - Pf6N2E2_1590 0.55 +1.6 1,860,996 - Pf6N2E2_1590 0.55 +0.5 1,861,017 + Pf6N2E2_1590 0.56 +0.9 1,861,018 - Pf6N2E2_1590 0.56 +0.4 1,861,018 - Pf6N2E2_1590 0.56 +1.5 1,861,018 - Pf6N2E2_1590 0.56 +1.3 1,861,018 - Pf6N2E2_1590 0.56 +0.7 1,861,018 - Pf6N2E2_1590 0.56 -0.1
Or see this region's nucleotide sequence