Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_144

Experiment: Choline Lysine 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_142 and Pf6N2E2_143 are separated by 177 nucleotidesPf6N2E2_143 and Pf6N2E2_144 are separated by 83 nucleotidesPf6N2E2_144 and Pf6N2E2_145 are separated by 17 nucleotides Pf6N2E2_142 - Exodeoxyribonuclease III (EC 3.1.11.2), at 193,732 to 194,544 _142 Pf6N2E2_143 - Histone acetyltransferase HPA2 and related acetyltransferases, at 194,722 to 195,372 _143 Pf6N2E2_144 - Uncharacterized protein YtfM precursor, at 195,456 to 197,165 _144 Pf6N2E2_145 - putative exported protein, at 197,183 to 200,836 _145 Position (kb) 195 196 197 198Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 194.574 kb on + strandat 194.574 kb on + strandat 194.575 kb on - strandat 194.575 kb on - strandat 194.575 kb on - strandat 194.641 kb on + strandat 194.641 kb on + strandat 194.641 kb on + strandat 194.642 kb on - strandat 194.670 kb on + strandat 194.671 kb on - strandat 194.677 kb on + strandat 194.757 kb on + strandat 194.757 kb on + strandat 194.757 kb on + strandat 194.758 kb on - strandat 194.773 kb on + strandat 194.801 kb on - strand, within Pf6N2E2_143at 194.801 kb on - strand, within Pf6N2E2_143at 194.801 kb on - strand, within Pf6N2E2_143at 194.801 kb on - strand, within Pf6N2E2_143at 194.833 kb on + strand, within Pf6N2E2_143at 194.833 kb on + strand, within Pf6N2E2_143at 194.834 kb on - strand, within Pf6N2E2_143at 194.834 kb on - strand, within Pf6N2E2_143at 194.834 kb on - strand, within Pf6N2E2_143at 194.834 kb on - strand, within Pf6N2E2_143at 195.074 kb on - strand, within Pf6N2E2_143at 195.074 kb on - strand, within Pf6N2E2_143at 195.119 kb on + strand, within Pf6N2E2_143at 195.119 kb on + strand, within Pf6N2E2_143at 195.119 kb on + strand, within Pf6N2E2_143at 195.119 kb on + strand, within Pf6N2E2_143at 195.119 kb on + strand, within Pf6N2E2_143at 195.120 kb on - strand, within Pf6N2E2_143at 195.120 kb on - strand, within Pf6N2E2_143at 195.120 kb on - strand, within Pf6N2E2_143at 195.192 kb on + strand, within Pf6N2E2_143at 195.192 kb on + strand, within Pf6N2E2_143at 195.192 kb on + strand, within Pf6N2E2_143at 195.192 kb on + strand, within Pf6N2E2_143at 195.192 kb on + strand, within Pf6N2E2_143at 195.192 kb on + strand, within Pf6N2E2_143at 195.193 kb on - strand, within Pf6N2E2_143at 195.193 kb on - strand, within Pf6N2E2_143at 195.193 kb on - strand, within Pf6N2E2_143at 195.254 kb on - strand, within Pf6N2E2_143at 195.254 kb on - strand, within Pf6N2E2_143at 195.254 kb on - strand, within Pf6N2E2_143at 195.254 kb on - strand, within Pf6N2E2_143at 195.265 kb on + strand, within Pf6N2E2_143at 195.266 kb on - strand, within Pf6N2E2_143at 195.268 kb on - strand, within Pf6N2E2_143at 195.281 kb on - strand, within Pf6N2E2_143at 195.371 kb on - strandat 195.414 kb on + strandat 195.520 kb on + strandat 195.520 kb on + strandat 195.521 kb on - strandat 195.521 kb on - strandat 195.559 kb on + strandat 195.559 kb on + strandat 195.559 kb on + strandat 195.559 kb on + strandat 195.559 kb on + strandat 195.559 kb on + strandat 195.559 kb on + strandat 195.559 kb on + strandat 195.560 kb on - strandat 195.560 kb on - strandat 195.560 kb on - strandat 195.560 kb on - strandat 195.560 kb on - strandat 195.560 kb on - strandat 195.560 kb on - strandat 195.560 kb on - strandat 195.560 kb on - strandat 195.560 kb on - strandat 195.560 kb on - strandat 195.560 kb on - strandat 195.560 kb on - strandat 195.567 kb on + strandat 195.567 kb on + strandat 195.567 kb on + strandat 195.567 kb on + strandat 195.567 kb on + strandat 195.567 kb on + strandat 195.567 kb on + strandat 195.567 kb on + strandat 195.567 kb on + strandat 195.567 kb on + strandat 195.567 kb on + strandat 195.568 kb on - strandat 195.568 kb on - strandat 195.568 kb on - strandat 195.568 kb on - strandat 195.568 kb on - strandat 195.569 kb on + strandat 195.570 kb on - strandat 195.570 kb on - strandat 195.664 kb on - strand, within Pf6N2E2_144at 195.666 kb on + strand, within Pf6N2E2_144at 195.666 kb on + strandat 195.982 kb on - strand, within Pf6N2E2_144at 195.999 kb on + strand, within Pf6N2E2_144at 195.999 kb on + strandat 195.999 kb on + strand, within Pf6N2E2_144at 196.000 kb on - strand, within Pf6N2E2_144at 196.089 kb on + strand, within Pf6N2E2_144at 196.090 kb on - strand, within Pf6N2E2_144at 196.090 kb on - strand, within Pf6N2E2_144at 196.141 kb on - strand, within Pf6N2E2_144at 196.326 kb on + strand, within Pf6N2E2_144at 196.326 kb on + strand, within Pf6N2E2_144at 196.326 kb on + strandat 196.327 kb on - strand, within Pf6N2E2_144at 196.327 kb on - strand, within Pf6N2E2_144at 196.377 kb on + strand, within Pf6N2E2_144at 196.378 kb on - strand, within Pf6N2E2_144at 196.414 kb on + strand, within Pf6N2E2_144at 196.414 kb on + strand, within Pf6N2E2_144at 196.414 kb on + strand, within Pf6N2E2_144at 196.414 kb on + strand, within Pf6N2E2_144at 196.414 kb on + strand, within Pf6N2E2_144at 196.414 kb on + strand, within Pf6N2E2_144at 196.414 kb on + strand, within Pf6N2E2_144at 196.414 kb on + strand, within Pf6N2E2_144at 196.414 kb on + strand, within Pf6N2E2_144at 196.414 kb on + strand, within Pf6N2E2_144at 196.414 kb on + strand, within Pf6N2E2_144at 196.414 kb on + strand, within Pf6N2E2_144at 196.415 kb on - strand, within Pf6N2E2_144at 196.415 kb on - strand, within Pf6N2E2_144at 196.415 kb on - strand, within Pf6N2E2_144at 196.415 kb on - strand, within Pf6N2E2_144at 196.415 kb on - strand, within Pf6N2E2_144at 196.415 kb on - strand, within Pf6N2E2_144at 196.415 kb on - strand, within Pf6N2E2_144at 196.415 kb on - strand, within Pf6N2E2_144at 196.415 kb on - strand, within Pf6N2E2_144at 196.415 kb on - strand, within Pf6N2E2_144at 196.437 kb on + strand, within Pf6N2E2_144at 196.437 kb on + strand, within Pf6N2E2_144at 196.437 kb on + strand, within Pf6N2E2_144at 196.437 kb on + strand, within Pf6N2E2_144at 196.437 kb on + strand, within Pf6N2E2_144at 196.438 kb on - strand, within Pf6N2E2_144at 196.438 kb on - strand, within Pf6N2E2_144at 196.438 kb on - strand, within Pf6N2E2_144at 196.438 kb on - strand, within Pf6N2E2_144at 196.438 kb on - strand, within Pf6N2E2_144at 196.557 kb on + strand, within Pf6N2E2_144at 196.611 kb on + strand, within Pf6N2E2_144at 196.611 kb on + strand, within Pf6N2E2_144at 196.611 kb on + strand, within Pf6N2E2_144at 196.612 kb on - strand, within Pf6N2E2_144at 196.612 kb on - strand, within Pf6N2E2_144at 196.612 kb on - strand, within Pf6N2E2_144at 196.617 kb on + strand, within Pf6N2E2_144at 196.617 kb on + strand, within Pf6N2E2_144at 196.617 kb on + strand, within Pf6N2E2_144at 196.617 kb on + strand, within Pf6N2E2_144at 196.617 kb on + strand, within Pf6N2E2_144at 196.618 kb on - strand, within Pf6N2E2_144at 196.618 kb on - strand, within Pf6N2E2_144at 196.717 kb on + strand, within Pf6N2E2_144at 196.717 kb on + strand, within Pf6N2E2_144at 196.717 kb on + strand, within Pf6N2E2_144at 196.718 kb on - strand, within Pf6N2E2_144at 196.719 kb on + strand, within Pf6N2E2_144at 196.719 kb on + strand, within Pf6N2E2_144at 196.720 kb on - strand, within Pf6N2E2_144at 196.720 kb on - strand, within Pf6N2E2_144at 196.865 kb on - strand, within Pf6N2E2_144at 196.865 kb on - strand, within Pf6N2E2_144at 196.929 kb on + strand, within Pf6N2E2_144at 196.929 kb on + strand, within Pf6N2E2_144at 196.929 kb on + strand, within Pf6N2E2_144at 196.929 kb on + strand, within Pf6N2E2_144at 196.929 kb on + strand, within Pf6N2E2_144at 196.929 kb on + strand, within Pf6N2E2_144at 196.929 kb on + strand, within Pf6N2E2_144at 196.930 kb on - strand, within Pf6N2E2_144at 196.930 kb on - strand, within Pf6N2E2_144at 196.930 kb on - strand, within Pf6N2E2_144at 196.930 kb on - strand, within Pf6N2E2_144at 196.930 kb on - strand, within Pf6N2E2_144at 196.930 kb on - strand, within Pf6N2E2_144at 196.930 kb on - strand, within Pf6N2E2_144at 196.930 kb on - strand, within Pf6N2E2_144at 196.954 kb on - strand, within Pf6N2E2_144at 196.959 kb on + strand, within Pf6N2E2_144at 196.959 kb on + strand, within Pf6N2E2_144at 196.960 kb on - strand, within Pf6N2E2_144at 196.960 kb on - strand, within Pf6N2E2_144at 196.960 kb on - strand, within Pf6N2E2_144at 197.015 kb on + strandat 197.015 kb on + strandat 197.016 kb on - strandat 197.016 kb on - strandat 197.181 kb on + strandat 197.182 kb on - strandat 197.182 kb on - strandat 197.376 kb on + strandat 197.822 kb on + strand, within Pf6N2E2_145at 197.823 kb on - strand, within Pf6N2E2_145at 197.823 kb on - strand, within Pf6N2E2_145

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline Lysine 20 mM
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194,574 + -1.5
194,574 + -1.7
194,575 - -0.1
194,575 - +0.1
194,575 - -0.8
194,641 + -0.8
194,641 + -0.1
194,641 + +0.7
194,642 - +0.7
194,670 + +0.7
194,671 - -0.6
194,677 + -0.0
194,757 + -0.8
194,757 + +0.7
194,757 + +0.2
194,758 - +0.1
194,773 + +1.3
194,801 - Pf6N2E2_143 0.12 -0.7
194,801 - Pf6N2E2_143 0.12 +0.3
194,801 - Pf6N2E2_143 0.12 -0.2
194,801 - Pf6N2E2_143 0.12 -0.2
194,833 + Pf6N2E2_143 0.17 -2.7
194,833 + Pf6N2E2_143 0.17 +0.7
194,834 - Pf6N2E2_143 0.17 -0.4
194,834 - Pf6N2E2_143 0.17 +0.1
194,834 - Pf6N2E2_143 0.17 -0.2
194,834 - Pf6N2E2_143 0.17 -0.5
195,074 - Pf6N2E2_143 0.54 -0.7
195,074 - Pf6N2E2_143 0.54 -0.5
195,119 + Pf6N2E2_143 0.61 +0.6
195,119 + Pf6N2E2_143 0.61 -0.4
195,119 + Pf6N2E2_143 0.61 +0.2
195,119 + Pf6N2E2_143 0.61 -0.4
195,119 + Pf6N2E2_143 0.61 -0.3
195,120 - Pf6N2E2_143 0.61 +0.1
195,120 - Pf6N2E2_143 0.61 +1.3
195,120 - Pf6N2E2_143 0.61 +0.1
195,192 + Pf6N2E2_143 0.72 +0.8
195,192 + Pf6N2E2_143 0.72 -1.2
195,192 + Pf6N2E2_143 0.72 -1.5
195,192 + Pf6N2E2_143 0.72 +0.2
195,192 + Pf6N2E2_143 0.72 +1.6
195,192 + Pf6N2E2_143 0.72 +0.5
195,193 - Pf6N2E2_143 0.72 +0.8
195,193 - Pf6N2E2_143 0.72 +1.2
195,193 - Pf6N2E2_143 0.72 +0.6
195,254 - Pf6N2E2_143 0.82 +1.2
195,254 - Pf6N2E2_143 0.82 +1.1
195,254 - Pf6N2E2_143 0.82 +0.8
195,254 - Pf6N2E2_143 0.82 +0.8
195,265 + Pf6N2E2_143 0.83 -0.8
195,266 - Pf6N2E2_143 0.84 +1.7
195,268 - Pf6N2E2_143 0.84 -0.1
195,281 - Pf6N2E2_143 0.86 -0.5
195,371 - -0.8
195,414 + -0.7
195,520 + -2.4
195,520 + -4.8
195,521 - -2.2
195,521 - -3.1
195,559 + -0.5
195,559 + -1.4
195,559 + -1.9
195,559 + -1.5
195,559 + -3.6
195,559 + -2.4
195,559 + -2.6
195,559 + -3.6
195,560 - -1.4
195,560 - -2.9
195,560 - -0.4
195,560 - -2.6
195,560 - +0.9
195,560 - -1.9
195,560 - -2.9
195,560 - -3.2
195,560 - -2.0
195,560 - -3.0
195,560 - -0.8
195,560 - -3.3
195,560 - -3.6
195,567 + -3.4
195,567 + -0.2
195,567 + -3.9
195,567 + -2.3
195,567 + -3.1
195,567 + -3.0
195,567 + -1.1
195,567 + -3.3
195,567 + -1.7
195,567 + -2.4
195,567 + -1.2
195,568 - -1.0
195,568 - -1.6
195,568 - -1.5
195,568 - -3.2
195,568 - -4.1
195,569 + -3.5
195,570 - -2.3
195,570 - -2.1
195,664 - Pf6N2E2_144 0.12 -1.4
195,666 + Pf6N2E2_144 0.12 -2.8
195,666 + -0.3
195,982 - Pf6N2E2_144 0.31 -0.7
195,999 + Pf6N2E2_144 0.32 -1.9
195,999 + +1.5
195,999 + Pf6N2E2_144 0.32 -1.7
196,000 - Pf6N2E2_144 0.32 -2.1
196,089 + Pf6N2E2_144 0.37 -1.8
196,090 - Pf6N2E2_144 0.37 -2.6
196,090 - Pf6N2E2_144 0.37 -3.9
196,141 - Pf6N2E2_144 0.40 -3.5
196,326 + Pf6N2E2_144 0.51 -2.7
196,326 + Pf6N2E2_144 0.51 -1.8
196,326 + -0.3
196,327 - Pf6N2E2_144 0.51 -3.0
196,327 - Pf6N2E2_144 0.51 -1.0
196,377 + Pf6N2E2_144 0.54 -1.2
196,378 - Pf6N2E2_144 0.54 -1.7
196,414 + Pf6N2E2_144 0.56 -0.7
196,414 + Pf6N2E2_144 0.56 -2.3
196,414 + Pf6N2E2_144 0.56 -2.4
196,414 + Pf6N2E2_144 0.56 -3.7
196,414 + Pf6N2E2_144 0.56 -2.0
196,414 + Pf6N2E2_144 0.56 -0.2
196,414 + Pf6N2E2_144 0.56 -2.6
196,414 + Pf6N2E2_144 0.56 -1.7
196,414 + Pf6N2E2_144 0.56 -4.3
196,414 + Pf6N2E2_144 0.56 -3.7
196,414 + Pf6N2E2_144 0.56 -1.6
196,414 + Pf6N2E2_144 0.56 -1.9
196,415 - Pf6N2E2_144 0.56 -2.4
196,415 - Pf6N2E2_144 0.56 -3.7
196,415 - Pf6N2E2_144 0.56 -4.1
196,415 - Pf6N2E2_144 0.56 -1.9
196,415 - Pf6N2E2_144 0.56 -1.8
196,415 - Pf6N2E2_144 0.56 -0.8
196,415 - Pf6N2E2_144 0.56 -1.5
196,415 - Pf6N2E2_144 0.56 -3.2
196,415 - Pf6N2E2_144 0.56 -3.4
196,415 - Pf6N2E2_144 0.56 -2.8
196,437 + Pf6N2E2_144 0.57 -0.3
196,437 + Pf6N2E2_144 0.57 -0.7
196,437 + Pf6N2E2_144 0.57 -1.0
196,437 + Pf6N2E2_144 0.57 -1.0
196,437 + Pf6N2E2_144 0.57 -0.1
196,438 - Pf6N2E2_144 0.57 -2.7
196,438 - Pf6N2E2_144 0.57 -2.5
196,438 - Pf6N2E2_144 0.57 -1.3
196,438 - Pf6N2E2_144 0.57 -1.7
196,438 - Pf6N2E2_144 0.57 -1.8
196,557 + Pf6N2E2_144 0.64 -3.1
196,611 + Pf6N2E2_144 0.68 -2.5
196,611 + Pf6N2E2_144 0.68 -0.4
196,611 + Pf6N2E2_144 0.68 -2.2
196,612 - Pf6N2E2_144 0.68 -3.2
196,612 - Pf6N2E2_144 0.68 -1.5
196,612 - Pf6N2E2_144 0.68 -1.9
196,617 + Pf6N2E2_144 0.68 -2.3
196,617 + Pf6N2E2_144 0.68 -0.3
196,617 + Pf6N2E2_144 0.68 -2.0
196,617 + Pf6N2E2_144 0.68 -1.8
196,617 + Pf6N2E2_144 0.68 -2.6
196,618 - Pf6N2E2_144 0.68 -1.6
196,618 - Pf6N2E2_144 0.68 -1.5
196,717 + Pf6N2E2_144 0.74 -1.6
196,717 + Pf6N2E2_144 0.74 -1.3
196,717 + Pf6N2E2_144 0.74 -2.8
196,718 - Pf6N2E2_144 0.74 -1.6
196,719 + Pf6N2E2_144 0.74 -1.2
196,719 + Pf6N2E2_144 0.74 -0.8
196,720 - Pf6N2E2_144 0.74 -3.3
196,720 - Pf6N2E2_144 0.74 -2.1
196,865 - Pf6N2E2_144 0.82 -1.1
196,865 - Pf6N2E2_144 0.82 -1.3
196,929 + Pf6N2E2_144 0.86 -2.6
196,929 + Pf6N2E2_144 0.86 -1.9
196,929 + Pf6N2E2_144 0.86 -4.3
196,929 + Pf6N2E2_144 0.86 -3.0
196,929 + Pf6N2E2_144 0.86 -2.4
196,929 + Pf6N2E2_144 0.86 -3.3
196,929 + Pf6N2E2_144 0.86 -2.2
196,930 - Pf6N2E2_144 0.86 -2.7
196,930 - Pf6N2E2_144 0.86 -0.6
196,930 - Pf6N2E2_144 0.86 -1.1
196,930 - Pf6N2E2_144 0.86 -4.0
196,930 - Pf6N2E2_144 0.86 -1.4
196,930 - Pf6N2E2_144 0.86 -2.0
196,930 - Pf6N2E2_144 0.86 -1.6
196,930 - Pf6N2E2_144 0.86 -3.2
196,954 - Pf6N2E2_144 0.88 -2.3
196,959 + Pf6N2E2_144 0.88 -3.3
196,959 + Pf6N2E2_144 0.88 -3.2
196,960 - Pf6N2E2_144 0.88 -2.3
196,960 - Pf6N2E2_144 0.88 -3.7
196,960 - Pf6N2E2_144 0.88 -3.9
197,015 + -2.3
197,015 + -1.4
197,016 - -1.1
197,016 - -2.3
197,181 + +0.4
197,182 - -0.3
197,182 - -4.3
197,376 + -1.1
197,822 + Pf6N2E2_145 0.17 -1.8
197,823 - Pf6N2E2_145 0.18 -1.1
197,823 - Pf6N2E2_145 0.18 -0.8

Or see this region's nucleotide sequence