Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1310

Experiment: Choline Lysine 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1309 and Pf6N2E2_1310 are separated by 13 nucleotidesPf6N2E2_1310 and Pf6N2E2_1311 are separated by 91 nucleotidesPf6N2E2_1311 and Pf6N2E2_1312 are separated by 52 nucleotides Pf6N2E2_1309 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60), at 1,542,921 to 1,544,381 _1309 Pf6N2E2_1310 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase (EC 1.13.11.15), at 1,544,395 to 1,545,426 _1310 Pf6N2E2_1311 - 5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10), at 1,545,518 to 1,545,910 _1311 Pf6N2E2_1312 - 4-hydroxyphenylacetate symporter, major facilitator superfamily (MFS), at 1,545,963 to 1,547,267 _1312 Position (kb) 1544 1545 1546Strain fitness (log2 ratio) -2 -1 0 1at 1543.501 kb on + strand, within Pf6N2E2_1309at 1543.565 kb on - strand, within Pf6N2E2_1309at 1543.908 kb on + strand, within Pf6N2E2_1309at 1544.136 kb on + strand, within Pf6N2E2_1309at 1544.151 kb on + strand, within Pf6N2E2_1309at 1544.152 kb on - strand, within Pf6N2E2_1309at 1544.152 kb on - strand, within Pf6N2E2_1309at 1544.301 kb on + strandat 1544.362 kb on + strandat 1544.362 kb on + strandat 1544.389 kb on + strandat 1544.390 kb on - strandat 1544.617 kb on + strand, within Pf6N2E2_1310at 1544.617 kb on + strand, within Pf6N2E2_1310at 1544.617 kb on + strand, within Pf6N2E2_1310at 1544.618 kb on - strand, within Pf6N2E2_1310at 1544.618 kb on - strand, within Pf6N2E2_1310at 1544.794 kb on + strand, within Pf6N2E2_1310at 1544.794 kb on + strand, within Pf6N2E2_1310at 1544.795 kb on - strand, within Pf6N2E2_1310at 1544.795 kb on - strand, within Pf6N2E2_1310at 1544.795 kb on - strand, within Pf6N2E2_1310at 1544.837 kb on - strand, within Pf6N2E2_1310at 1544.837 kb on - strand, within Pf6N2E2_1310at 1544.837 kb on - strand, within Pf6N2E2_1310at 1544.842 kb on + strand, within Pf6N2E2_1310at 1544.965 kb on + strand, within Pf6N2E2_1310at 1544.966 kb on - strand, within Pf6N2E2_1310at 1544.966 kb on - strand, within Pf6N2E2_1310at 1545.347 kb on - strandat 1545.450 kb on - strandat 1545.450 kb on - strandat 1545.480 kb on + strandat 1545.703 kb on - strand, within Pf6N2E2_1311at 1545.703 kb on - strand, within Pf6N2E2_1311at 1546.063 kb on - strandat 1546.311 kb on + strand, within Pf6N2E2_1312

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline Lysine 20 mM
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1,543,501 + Pf6N2E2_1309 0.40 +0.5
1,543,565 - Pf6N2E2_1309 0.44 +0.1
1,543,908 + Pf6N2E2_1309 0.68 +0.3
1,544,136 + Pf6N2E2_1309 0.83 -0.4
1,544,151 + Pf6N2E2_1309 0.84 -0.2
1,544,152 - Pf6N2E2_1309 0.84 -1.1
1,544,152 - Pf6N2E2_1309 0.84 +0.2
1,544,301 + -1.5
1,544,362 + +0.6
1,544,362 + +0.9
1,544,389 + +0.7
1,544,390 - -0.2
1,544,617 + Pf6N2E2_1310 0.22 -1.1
1,544,617 + Pf6N2E2_1310 0.22 +0.1
1,544,617 + Pf6N2E2_1310 0.22 -0.4
1,544,618 - Pf6N2E2_1310 0.22 -0.9
1,544,618 - Pf6N2E2_1310 0.22 +0.7
1,544,794 + Pf6N2E2_1310 0.39 +0.7
1,544,794 + Pf6N2E2_1310 0.39 -0.7
1,544,795 - Pf6N2E2_1310 0.39 +1.2
1,544,795 - Pf6N2E2_1310 0.39 -0.3
1,544,795 - Pf6N2E2_1310 0.39 -2.4
1,544,837 - Pf6N2E2_1310 0.43 -0.4
1,544,837 - Pf6N2E2_1310 0.43 +0.0
1,544,837 - Pf6N2E2_1310 0.43 +0.1
1,544,842 + Pf6N2E2_1310 0.43 -0.3
1,544,965 + Pf6N2E2_1310 0.55 +1.1
1,544,966 - Pf6N2E2_1310 0.55 -0.1
1,544,966 - Pf6N2E2_1310 0.55 -1.7
1,545,347 - +0.1
1,545,450 - +0.4
1,545,450 - +1.0
1,545,480 + -1.6
1,545,703 - Pf6N2E2_1311 0.47 -0.8
1,545,703 - Pf6N2E2_1311 0.47 -0.9
1,546,063 - +0.6
1,546,311 + Pf6N2E2_1312 0.27 -1.8

Or see this region's nucleotide sequence