Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1309 and Pf6N2E2_1310 are separated by 13 nucleotides Pf6N2E2_1310 and Pf6N2E2_1311 are separated by 91 nucleotides Pf6N2E2_1311 and Pf6N2E2_1312 are separated by 52 nucleotides
Pf6N2E2_1309 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60), at 1,542,921 to 1,544,381
_1309
Pf6N2E2_1310 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase (EC 1.13.11.15), at 1,544,395 to 1,545,426
_1310
Pf6N2E2_1311 - 5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10), at 1,545,518 to 1,545,910
_1311
Pf6N2E2_1312 - 4-hydroxyphenylacetate symporter, major facilitator superfamily (MFS), at 1,545,963 to 1,547,267
_1312
Position (kb)
1544
1545
1546 Strain fitness (log2 ratio)
-2
-1
0
1 at 1543.501 kb on + strand, within Pf6N2E2_1309 at 1543.565 kb on - strand, within Pf6N2E2_1309 at 1543.908 kb on + strand, within Pf6N2E2_1309 at 1544.136 kb on + strand, within Pf6N2E2_1309 at 1544.151 kb on + strand, within Pf6N2E2_1309 at 1544.152 kb on - strand, within Pf6N2E2_1309 at 1544.152 kb on - strand, within Pf6N2E2_1309 at 1544.301 kb on + strand at 1544.362 kb on + strand at 1544.362 kb on + strand at 1544.389 kb on + strand at 1544.390 kb on - strand at 1544.617 kb on + strand, within Pf6N2E2_1310 at 1544.617 kb on + strand, within Pf6N2E2_1310 at 1544.617 kb on + strand, within Pf6N2E2_1310 at 1544.618 kb on - strand, within Pf6N2E2_1310 at 1544.618 kb on - strand, within Pf6N2E2_1310 at 1544.794 kb on + strand, within Pf6N2E2_1310 at 1544.794 kb on + strand, within Pf6N2E2_1310 at 1544.795 kb on - strand, within Pf6N2E2_1310 at 1544.795 kb on - strand, within Pf6N2E2_1310 at 1544.795 kb on - strand, within Pf6N2E2_1310 at 1544.837 kb on - strand, within Pf6N2E2_1310 at 1544.837 kb on - strand, within Pf6N2E2_1310 at 1544.837 kb on - strand, within Pf6N2E2_1310 at 1544.842 kb on + strand, within Pf6N2E2_1310 at 1544.965 kb on + strand, within Pf6N2E2_1310 at 1544.966 kb on - strand, within Pf6N2E2_1310 at 1544.966 kb on - strand, within Pf6N2E2_1310 at 1545.347 kb on - strand at 1545.450 kb on - strand at 1545.450 kb on - strand at 1545.480 kb on + strand at 1545.703 kb on - strand, within Pf6N2E2_1311 at 1545.703 kb on - strand, within Pf6N2E2_1311 at 1546.063 kb on - strand at 1546.311 kb on + strand, within Pf6N2E2_1312
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 1,543,501 + Pf6N2E2_1309 0.40 +0.5 1,543,565 - Pf6N2E2_1309 0.44 +0.1 1,543,908 + Pf6N2E2_1309 0.68 +0.3 1,544,136 + Pf6N2E2_1309 0.83 -0.4 1,544,151 + Pf6N2E2_1309 0.84 -0.2 1,544,152 - Pf6N2E2_1309 0.84 -1.1 1,544,152 - Pf6N2E2_1309 0.84 +0.2 1,544,301 + -1.5 1,544,362 + +0.6 1,544,362 + +0.9 1,544,389 + +0.7 1,544,390 - -0.2 1,544,617 + Pf6N2E2_1310 0.22 -1.1 1,544,617 + Pf6N2E2_1310 0.22 +0.1 1,544,617 + Pf6N2E2_1310 0.22 -0.4 1,544,618 - Pf6N2E2_1310 0.22 -0.9 1,544,618 - Pf6N2E2_1310 0.22 +0.7 1,544,794 + Pf6N2E2_1310 0.39 +0.7 1,544,794 + Pf6N2E2_1310 0.39 -0.7 1,544,795 - Pf6N2E2_1310 0.39 +1.2 1,544,795 - Pf6N2E2_1310 0.39 -0.3 1,544,795 - Pf6N2E2_1310 0.39 -2.4 1,544,837 - Pf6N2E2_1310 0.43 -0.4 1,544,837 - Pf6N2E2_1310 0.43 +0.0 1,544,837 - Pf6N2E2_1310 0.43 +0.1 1,544,842 + Pf6N2E2_1310 0.43 -0.3 1,544,965 + Pf6N2E2_1310 0.55 +1.1 1,544,966 - Pf6N2E2_1310 0.55 -0.1 1,544,966 - Pf6N2E2_1310 0.55 -1.7 1,545,347 - +0.1 1,545,450 - +0.4 1,545,450 - +1.0 1,545,480 + -1.6 1,545,703 - Pf6N2E2_1311 0.47 -0.8 1,545,703 - Pf6N2E2_1311 0.47 -0.9 1,546,063 - +0.6 1,546,311 + Pf6N2E2_1312 0.27 -1.8
Or see this region's nucleotide sequence