Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1271 and Pf6N2E2_1272 are separated by 190 nucleotides Pf6N2E2_1272 and Pf6N2E2_1273 overlap by 4 nucleotides
Pf6N2E2_1271 - Acriflavin resistance plasma membrane protein, at 1,497,668 to 1,500,772
_1271
Pf6N2E2_1272 - Acriflavin resistance plasma membrane protein, at 1,500,963 to 1,504,058
_1272
Pf6N2E2_1273 - Probable RND efflux membrane fusion protein, at 1,504,055 to 1,505,356
_1273
Position (kb)
1500
1501
1502
1503
1504
1505 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1499.964 kb on + strand, within Pf6N2E2_1271 at 1499.965 kb on - strand, within Pf6N2E2_1271 at 1500.184 kb on - strand, within Pf6N2E2_1271 at 1500.248 kb on + strand, within Pf6N2E2_1271 at 1500.248 kb on + strand, within Pf6N2E2_1271 at 1500.249 kb on - strand, within Pf6N2E2_1271 at 1500.249 kb on - strand, within Pf6N2E2_1271 at 1500.266 kb on + strand, within Pf6N2E2_1271 at 1500.310 kb on - strand, within Pf6N2E2_1271 at 1500.310 kb on - strand, within Pf6N2E2_1271 at 1500.385 kb on + strand, within Pf6N2E2_1271 at 1500.387 kb on + strand, within Pf6N2E2_1271 at 1500.387 kb on + strand, within Pf6N2E2_1271 at 1500.388 kb on - strand, within Pf6N2E2_1271 at 1500.388 kb on - strand, within Pf6N2E2_1271 at 1500.397 kb on + strand, within Pf6N2E2_1271 at 1500.508 kb on + strand at 1500.509 kb on - strand at 1500.941 kb on + strand at 1500.942 kb on - strand at 1500.942 kb on - strand at 1500.942 kb on - strand at 1500.942 kb on - strand at 1501.022 kb on - strand at 1501.027 kb on + strand at 1501.028 kb on - strand at 1501.028 kb on - strand at 1501.143 kb on + strand at 1501.143 kb on + strand at 1501.143 kb on + strand at 1501.144 kb on - strand at 1501.144 kb on - strand at 1501.144 kb on - strand at 1501.197 kb on + strand at 1501.210 kb on + strand at 1501.210 kb on + strand at 1501.211 kb on - strand at 1501.446 kb on + strand, within Pf6N2E2_1272 at 1501.446 kb on + strand, within Pf6N2E2_1272 at 1501.447 kb on - strand, within Pf6N2E2_1272 at 1501.448 kb on + strand, within Pf6N2E2_1272 at 1501.448 kb on + strand, within Pf6N2E2_1272 at 1501.448 kb on + strand, within Pf6N2E2_1272 at 1501.448 kb on + strand, within Pf6N2E2_1272 at 1501.448 kb on + strand, within Pf6N2E2_1272 at 1501.448 kb on + strand, within Pf6N2E2_1272 at 1501.449 kb on - strand, within Pf6N2E2_1272 at 1501.449 kb on - strand, within Pf6N2E2_1272 at 1501.449 kb on - strand, within Pf6N2E2_1272 at 1501.450 kb on + strand, within Pf6N2E2_1272 at 1501.450 kb on + strand, within Pf6N2E2_1272 at 1501.450 kb on + strand, within Pf6N2E2_1272 at 1501.450 kb on + strand, within Pf6N2E2_1272 at 1501.450 kb on + strand, within Pf6N2E2_1272 at 1501.450 kb on + strand, within Pf6N2E2_1272 at 1501.450 kb on + strand, within Pf6N2E2_1272 at 1501.450 kb on + strand, within Pf6N2E2_1272 at 1501.450 kb on + strand, within Pf6N2E2_1272 at 1501.450 kb on + strand, within Pf6N2E2_1272 at 1501.450 kb on + strand, within Pf6N2E2_1272 at 1501.450 kb on + strand, within Pf6N2E2_1272 at 1501.450 kb on + strand, within Pf6N2E2_1272 at 1501.451 kb on - strand, within Pf6N2E2_1272 at 1501.451 kb on - strand, within Pf6N2E2_1272 at 1501.451 kb on - strand, within Pf6N2E2_1272 at 1501.451 kb on - strand, within Pf6N2E2_1272 at 1501.451 kb on - strand, within Pf6N2E2_1272 at 1501.451 kb on - strand, within Pf6N2E2_1272 at 1501.451 kb on - strand, within Pf6N2E2_1272 at 1501.451 kb on - strand, within Pf6N2E2_1272 at 1501.487 kb on + strand, within Pf6N2E2_1272 at 1501.488 kb on - strand, within Pf6N2E2_1272 at 1501.713 kb on + strand, within Pf6N2E2_1272 at 1501.822 kb on + strand, within Pf6N2E2_1272 at 1501.822 kb on + strand, within Pf6N2E2_1272 at 1501.823 kb on - strand, within Pf6N2E2_1272 at 1501.823 kb on - strand, within Pf6N2E2_1272 at 1501.823 kb on - strand, within Pf6N2E2_1272 at 1501.858 kb on + strand, within Pf6N2E2_1272 at 1501.859 kb on - strand, within Pf6N2E2_1272 at 1501.859 kb on - strand, within Pf6N2E2_1272 at 1502.046 kb on + strand, within Pf6N2E2_1272 at 1502.422 kb on + strand, within Pf6N2E2_1272 at 1502.440 kb on - strand, within Pf6N2E2_1272 at 1502.903 kb on + strand, within Pf6N2E2_1272 at 1502.927 kb on - strand, within Pf6N2E2_1272 at 1503.045 kb on + strand, within Pf6N2E2_1272 at 1503.045 kb on + strand, within Pf6N2E2_1272 at 1503.317 kb on - strand, within Pf6N2E2_1272 at 1503.443 kb on + strand, within Pf6N2E2_1272 at 1503.443 kb on + strand, within Pf6N2E2_1272 at 1503.674 kb on - strand, within Pf6N2E2_1272 at 1503.785 kb on + strand at 1504.255 kb on + strand, within Pf6N2E2_1273 at 1504.256 kb on - strand, within Pf6N2E2_1273 at 1504.324 kb on - strand, within Pf6N2E2_1273 at 1504.815 kb on + strand, within Pf6N2E2_1273 at 1504.816 kb on - strand, within Pf6N2E2_1273 at 1504.901 kb on + strand, within Pf6N2E2_1273 at 1504.972 kb on - strand, within Pf6N2E2_1273
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 1,499,964 + Pf6N2E2_1271 0.74 -1.4 1,499,965 - Pf6N2E2_1271 0.74 -1.1 1,500,184 - Pf6N2E2_1271 0.81 -1.8 1,500,248 + Pf6N2E2_1271 0.83 -1.3 1,500,248 + Pf6N2E2_1271 0.83 -2.5 1,500,249 - Pf6N2E2_1271 0.83 -1.7 1,500,249 - Pf6N2E2_1271 0.83 -1.7 1,500,266 + Pf6N2E2_1271 0.84 -0.2 1,500,310 - Pf6N2E2_1271 0.85 -2.2 1,500,310 - Pf6N2E2_1271 0.85 -2.8 1,500,385 + Pf6N2E2_1271 0.88 -2.5 1,500,387 + Pf6N2E2_1271 0.88 -0.4 1,500,387 + Pf6N2E2_1271 0.88 -1.5 1,500,388 - Pf6N2E2_1271 0.88 -1.4 1,500,388 - Pf6N2E2_1271 0.88 -2.1 1,500,397 + Pf6N2E2_1271 0.88 -1.8 1,500,508 + -1.9 1,500,509 - -2.0 1,500,941 + -2.3 1,500,942 - +0.4 1,500,942 - -1.1 1,500,942 - +1.1 1,500,942 - +0.4 1,501,022 - -2.0 1,501,027 + +0.5 1,501,028 - -1.7 1,501,028 - +0.9 1,501,143 + -1.1 1,501,143 + -2.7 1,501,143 + +0.5 1,501,144 - -1.2 1,501,144 - -1.3 1,501,144 - -3.9 1,501,197 + -0.8 1,501,210 + -3.3 1,501,210 + -1.1 1,501,211 - -2.0 1,501,446 + Pf6N2E2_1272 0.16 -0.6 1,501,446 + Pf6N2E2_1272 0.16 -0.2 1,501,447 - Pf6N2E2_1272 0.16 -2.0 1,501,448 + Pf6N2E2_1272 0.16 -0.1 1,501,448 + Pf6N2E2_1272 0.16 -0.3 1,501,448 + Pf6N2E2_1272 0.16 -0.4 1,501,448 + Pf6N2E2_1272 0.16 -1.0 1,501,448 + Pf6N2E2_1272 0.16 -2.2 1,501,448 + Pf6N2E2_1272 0.16 -2.1 1,501,449 - Pf6N2E2_1272 0.16 -1.6 1,501,449 - Pf6N2E2_1272 0.16 +0.2 1,501,449 - Pf6N2E2_1272 0.16 -1.5 1,501,450 + Pf6N2E2_1272 0.16 -1.4 1,501,450 + Pf6N2E2_1272 0.16 -2.5 1,501,450 + Pf6N2E2_1272 0.16 -0.9 1,501,450 + Pf6N2E2_1272 0.16 -0.9 1,501,450 + Pf6N2E2_1272 0.16 -1.6 1,501,450 + Pf6N2E2_1272 0.16 -3.0 1,501,450 + Pf6N2E2_1272 0.16 +0.6 1,501,450 + Pf6N2E2_1272 0.16 -2.3 1,501,450 + Pf6N2E2_1272 0.16 -1.5 1,501,450 + Pf6N2E2_1272 0.16 -0.4 1,501,450 + Pf6N2E2_1272 0.16 -0.3 1,501,450 + Pf6N2E2_1272 0.16 -1.7 1,501,450 + Pf6N2E2_1272 0.16 -1.7 1,501,451 - Pf6N2E2_1272 0.16 +0.4 1,501,451 - Pf6N2E2_1272 0.16 -0.8 1,501,451 - Pf6N2E2_1272 0.16 -0.3 1,501,451 - Pf6N2E2_1272 0.16 -2.1 1,501,451 - Pf6N2E2_1272 0.16 -1.6 1,501,451 - Pf6N2E2_1272 0.16 -1.4 1,501,451 - Pf6N2E2_1272 0.16 -1.3 1,501,451 - Pf6N2E2_1272 0.16 -2.3 1,501,487 + Pf6N2E2_1272 0.17 -2.7 1,501,488 - Pf6N2E2_1272 0.17 -0.2 1,501,713 + Pf6N2E2_1272 0.24 -1.3 1,501,822 + Pf6N2E2_1272 0.28 -0.8 1,501,822 + Pf6N2E2_1272 0.28 -2.2 1,501,823 - Pf6N2E2_1272 0.28 -2.4 1,501,823 - Pf6N2E2_1272 0.28 -1.5 1,501,823 - Pf6N2E2_1272 0.28 -1.7 1,501,858 + Pf6N2E2_1272 0.29 -0.8 1,501,859 - Pf6N2E2_1272 0.29 -1.0 1,501,859 - Pf6N2E2_1272 0.29 -2.2 1,502,046 + Pf6N2E2_1272 0.35 -0.7 1,502,422 + Pf6N2E2_1272 0.47 -1.4 1,502,440 - Pf6N2E2_1272 0.48 -1.6 1,502,903 + Pf6N2E2_1272 0.63 -1.7 1,502,927 - Pf6N2E2_1272 0.63 -0.0 1,503,045 + Pf6N2E2_1272 0.67 -2.1 1,503,045 + Pf6N2E2_1272 0.67 -1.5 1,503,317 - Pf6N2E2_1272 0.76 -2.6 1,503,443 + Pf6N2E2_1272 0.80 -0.7 1,503,443 + Pf6N2E2_1272 0.80 -1.1 1,503,674 - Pf6N2E2_1272 0.88 -0.8 1,503,785 + -3.8 1,504,255 + Pf6N2E2_1273 0.15 -3.4 1,504,256 - Pf6N2E2_1273 0.15 +0.1 1,504,324 - Pf6N2E2_1273 0.21 -1.5 1,504,815 + Pf6N2E2_1273 0.58 -0.6 1,504,816 - Pf6N2E2_1273 0.58 -2.2 1,504,901 + Pf6N2E2_1273 0.65 -2.6 1,504,972 - Pf6N2E2_1273 0.70 -0.4
Or see this region's nucleotide sequence