Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1269 and Pf6N2E2_1270 are separated by 294 nucleotides Pf6N2E2_1270 and Pf6N2E2_1271 overlap by 4 nucleotides
Pf6N2E2_1269 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17), at 1,495,089 to 1,495,901
_1269
Pf6N2E2_1270 - Heavy metal RND efflux outer membrane protein, CzcC family, at 1,496,196 to 1,497,671
_1270
Pf6N2E2_1271 - Acriflavin resistance plasma membrane protein, at 1,497,668 to 1,500,772
_1271
Position (kb)
1496
1497
1498 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1495.222 kb on + strand at 1495.222 kb on + strand, within Pf6N2E2_1269 at 1495.222 kb on + strand, within Pf6N2E2_1269 at 1495.223 kb on - strand, within Pf6N2E2_1269 at 1495.223 kb on - strand, within Pf6N2E2_1269 at 1495.223 kb on - strand, within Pf6N2E2_1269 at 1495.223 kb on - strand, within Pf6N2E2_1269 at 1495.324 kb on + strand, within Pf6N2E2_1269 at 1495.325 kb on - strand, within Pf6N2E2_1269 at 1495.325 kb on - strand, within Pf6N2E2_1269 at 1495.325 kb on - strand, within Pf6N2E2_1269 at 1495.383 kb on - strand, within Pf6N2E2_1269 at 1495.385 kb on - strand, within Pf6N2E2_1269 at 1495.656 kb on + strand, within Pf6N2E2_1269 at 1495.656 kb on + strand, within Pf6N2E2_1269 at 1495.657 kb on - strand, within Pf6N2E2_1269 at 1495.704 kb on + strand, within Pf6N2E2_1269 at 1495.867 kb on - strand at 1495.892 kb on - strand at 1495.892 kb on - strand at 1495.939 kb on + strand at 1495.939 kb on + strand at 1495.939 kb on + strand at 1495.939 kb on + strand at 1495.940 kb on - strand at 1495.940 kb on - strand at 1495.965 kb on - strand at 1496.166 kb on - strand at 1496.169 kb on - strand at 1496.342 kb on - strand at 1496.342 kb on - strand at 1496.342 kb on - strand at 1496.363 kb on - strand, within Pf6N2E2_1270 at 1496.459 kb on - strand, within Pf6N2E2_1270 at 1496.459 kb on - strand, within Pf6N2E2_1270 at 1496.500 kb on + strand, within Pf6N2E2_1270 at 1496.500 kb on + strand, within Pf6N2E2_1270 at 1496.521 kb on + strand, within Pf6N2E2_1270 at 1496.522 kb on - strand, within Pf6N2E2_1270 at 1496.535 kb on - strand, within Pf6N2E2_1270 at 1496.635 kb on + strand, within Pf6N2E2_1270 at 1496.636 kb on - strand, within Pf6N2E2_1270 at 1496.639 kb on + strand, within Pf6N2E2_1270 at 1496.639 kb on + strand, within Pf6N2E2_1270 at 1496.650 kb on + strand at 1496.686 kb on + strand, within Pf6N2E2_1270 at 1496.686 kb on + strand, within Pf6N2E2_1270 at 1497.017 kb on - strand, within Pf6N2E2_1270 at 1497.017 kb on - strand, within Pf6N2E2_1270 at 1497.076 kb on + strand, within Pf6N2E2_1270 at 1497.077 kb on - strand, within Pf6N2E2_1270 at 1497.084 kb on + strand, within Pf6N2E2_1270 at 1497.186 kb on + strand, within Pf6N2E2_1270 at 1497.186 kb on + strand, within Pf6N2E2_1270 at 1497.186 kb on + strand, within Pf6N2E2_1270 at 1497.186 kb on + strand, within Pf6N2E2_1270 at 1497.186 kb on + strand, within Pf6N2E2_1270 at 1497.187 kb on - strand, within Pf6N2E2_1270 at 1497.187 kb on - strand, within Pf6N2E2_1270 at 1497.187 kb on - strand, within Pf6N2E2_1270 at 1497.187 kb on - strand, within Pf6N2E2_1270 at 1497.223 kb on + strand, within Pf6N2E2_1270 at 1497.223 kb on + strand, within Pf6N2E2_1270 at 1497.223 kb on + strand, within Pf6N2E2_1270 at 1497.223 kb on + strand, within Pf6N2E2_1270 at 1497.223 kb on + strand, within Pf6N2E2_1270 at 1497.223 kb on + strand, within Pf6N2E2_1270 at 1497.520 kb on + strand, within Pf6N2E2_1270 at 1497.520 kb on + strand, within Pf6N2E2_1270 at 1497.521 kb on - strand, within Pf6N2E2_1270 at 1497.745 kb on - strand at 1497.751 kb on - strand at 1497.768 kb on + strand at 1497.768 kb on + strand at 1497.769 kb on - strand at 1498.074 kb on + strand, within Pf6N2E2_1271 at 1498.114 kb on + strand, within Pf6N2E2_1271 at 1498.115 kb on - strand, within Pf6N2E2_1271 at 1498.115 kb on - strand, within Pf6N2E2_1271 at 1498.141 kb on - strand, within Pf6N2E2_1271 at 1498.141 kb on - strand, within Pf6N2E2_1271 at 1498.149 kb on + strand, within Pf6N2E2_1271 at 1498.149 kb on + strand, within Pf6N2E2_1271 at 1498.170 kb on + strand, within Pf6N2E2_1271 at 1498.170 kb on + strand, within Pf6N2E2_1271 at 1498.171 kb on - strand, within Pf6N2E2_1271 at 1498.171 kb on - strand, within Pf6N2E2_1271 at 1498.410 kb on + strand, within Pf6N2E2_1271 at 1498.411 kb on - strand, within Pf6N2E2_1271 at 1498.478 kb on - strand, within Pf6N2E2_1271 at 1498.478 kb on - strand, within Pf6N2E2_1271 at 1498.494 kb on + strand, within Pf6N2E2_1271 at 1498.494 kb on + strand, within Pf6N2E2_1271 at 1498.494 kb on + strand, within Pf6N2E2_1271 at 1498.495 kb on - strand, within Pf6N2E2_1271 at 1498.495 kb on - strand, within Pf6N2E2_1271 at 1498.495 kb on - strand, within Pf6N2E2_1271 at 1498.518 kb on - strand, within Pf6N2E2_1271 at 1498.518 kb on - strand, within Pf6N2E2_1271 at 1498.525 kb on - strand at 1498.542 kb on + strand, within Pf6N2E2_1271 at 1498.542 kb on + strand, within Pf6N2E2_1271 at 1498.542 kb on + strand, within Pf6N2E2_1271 at 1498.543 kb on - strand, within Pf6N2E2_1271 at 1498.543 kb on - strand, within Pf6N2E2_1271 at 1498.543 kb on - strand, within Pf6N2E2_1271 at 1498.543 kb on - strand, within Pf6N2E2_1271 at 1498.543 kb on - strand, within Pf6N2E2_1271 at 1498.570 kb on - strand, within Pf6N2E2_1271 at 1498.570 kb on - strand, within Pf6N2E2_1271
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 1,495,222 + -0.5 1,495,222 + Pf6N2E2_1269 0.16 -0.1 1,495,222 + Pf6N2E2_1269 0.16 -0.0 1,495,223 - Pf6N2E2_1269 0.16 +0.7 1,495,223 - Pf6N2E2_1269 0.16 -0.7 1,495,223 - Pf6N2E2_1269 0.16 +0.5 1,495,223 - Pf6N2E2_1269 0.16 -0.5 1,495,324 + Pf6N2E2_1269 0.29 +0.3 1,495,325 - Pf6N2E2_1269 0.29 +0.5 1,495,325 - Pf6N2E2_1269 0.29 -0.5 1,495,325 - Pf6N2E2_1269 0.29 +1.0 1,495,383 - Pf6N2E2_1269 0.36 -0.1 1,495,385 - Pf6N2E2_1269 0.36 +0.5 1,495,656 + Pf6N2E2_1269 0.70 +0.6 1,495,656 + Pf6N2E2_1269 0.70 -2.5 1,495,657 - Pf6N2E2_1269 0.70 -0.2 1,495,704 + Pf6N2E2_1269 0.76 +0.9 1,495,867 - +0.5 1,495,892 - +0.2 1,495,892 - -1.5 1,495,939 + +0.9 1,495,939 + -0.1 1,495,939 + -0.8 1,495,939 + +0.8 1,495,940 - +1.3 1,495,940 - -1.5 1,495,965 - -2.1 1,496,166 - -0.6 1,496,169 - +1.2 1,496,342 - -1.1 1,496,342 - -1.1 1,496,342 - -1.2 1,496,363 - Pf6N2E2_1270 0.11 +0.0 1,496,459 - Pf6N2E2_1270 0.18 -0.1 1,496,459 - Pf6N2E2_1270 0.18 -1.1 1,496,500 + Pf6N2E2_1270 0.21 -2.0 1,496,500 + Pf6N2E2_1270 0.21 -3.4 1,496,521 + Pf6N2E2_1270 0.22 -0.7 1,496,522 - Pf6N2E2_1270 0.22 -1.0 1,496,535 - Pf6N2E2_1270 0.23 -2.3 1,496,635 + Pf6N2E2_1270 0.30 -1.6 1,496,636 - Pf6N2E2_1270 0.30 -0.9 1,496,639 + Pf6N2E2_1270 0.30 -0.7 1,496,639 + Pf6N2E2_1270 0.30 -1.1 1,496,650 + +0.5 1,496,686 + Pf6N2E2_1270 0.33 -0.5 1,496,686 + Pf6N2E2_1270 0.33 -0.3 1,497,017 - Pf6N2E2_1270 0.56 -1.7 1,497,017 - Pf6N2E2_1270 0.56 -0.7 1,497,076 + Pf6N2E2_1270 0.60 -1.6 1,497,077 - Pf6N2E2_1270 0.60 -0.9 1,497,084 + Pf6N2E2_1270 0.60 -3.3 1,497,186 + Pf6N2E2_1270 0.67 -1.8 1,497,186 + Pf6N2E2_1270 0.67 -1.8 1,497,186 + Pf6N2E2_1270 0.67 -1.3 1,497,186 + Pf6N2E2_1270 0.67 -1.7 1,497,186 + Pf6N2E2_1270 0.67 -1.6 1,497,187 - Pf6N2E2_1270 0.67 -2.3 1,497,187 - Pf6N2E2_1270 0.67 -1.9 1,497,187 - Pf6N2E2_1270 0.67 -1.2 1,497,187 - Pf6N2E2_1270 0.67 -2.2 1,497,223 + Pf6N2E2_1270 0.70 -1.8 1,497,223 + Pf6N2E2_1270 0.70 -1.1 1,497,223 + Pf6N2E2_1270 0.70 -2.9 1,497,223 + Pf6N2E2_1270 0.70 -2.4 1,497,223 + Pf6N2E2_1270 0.70 -0.9 1,497,223 + Pf6N2E2_1270 0.70 -2.2 1,497,520 + Pf6N2E2_1270 0.90 -3.2 1,497,520 + Pf6N2E2_1270 0.90 -1.2 1,497,521 - Pf6N2E2_1270 0.90 -0.1 1,497,745 - -1.5 1,497,751 - -1.6 1,497,768 + -2.0 1,497,768 + -1.5 1,497,769 - -2.5 1,498,074 + Pf6N2E2_1271 0.13 -0.4 1,498,114 + Pf6N2E2_1271 0.14 -1.8 1,498,115 - Pf6N2E2_1271 0.14 -0.5 1,498,115 - Pf6N2E2_1271 0.14 -0.1 1,498,141 - Pf6N2E2_1271 0.15 -1.9 1,498,141 - Pf6N2E2_1271 0.15 -0.8 1,498,149 + Pf6N2E2_1271 0.15 -1.8 1,498,149 + Pf6N2E2_1271 0.15 -0.8 1,498,170 + Pf6N2E2_1271 0.16 -1.8 1,498,170 + Pf6N2E2_1271 0.16 -1.3 1,498,171 - Pf6N2E2_1271 0.16 -0.1 1,498,171 - Pf6N2E2_1271 0.16 -1.5 1,498,410 + Pf6N2E2_1271 0.24 -1.0 1,498,411 - Pf6N2E2_1271 0.24 +0.3 1,498,478 - Pf6N2E2_1271 0.26 -2.4 1,498,478 - Pf6N2E2_1271 0.26 -1.1 1,498,494 + Pf6N2E2_1271 0.27 -1.1 1,498,494 + Pf6N2E2_1271 0.27 -2.6 1,498,494 + Pf6N2E2_1271 0.27 -2.0 1,498,495 - Pf6N2E2_1271 0.27 -1.1 1,498,495 - Pf6N2E2_1271 0.27 -1.1 1,498,495 - Pf6N2E2_1271 0.27 -1.6 1,498,518 - Pf6N2E2_1271 0.27 -3.3 1,498,518 - Pf6N2E2_1271 0.27 -2.2 1,498,525 - -0.7 1,498,542 + Pf6N2E2_1271 0.28 -2.3 1,498,542 + Pf6N2E2_1271 0.28 -3.6 1,498,542 + Pf6N2E2_1271 0.28 -1.4 1,498,543 - Pf6N2E2_1271 0.28 -0.2 1,498,543 - Pf6N2E2_1271 0.28 -1.7 1,498,543 - Pf6N2E2_1271 0.28 +0.1 1,498,543 - Pf6N2E2_1271 0.28 -1.6 1,498,543 - Pf6N2E2_1271 0.28 -2.0 1,498,570 - Pf6N2E2_1271 0.29 -2.4 1,498,570 - Pf6N2E2_1271 0.29 -2.2
Or see this region's nucleotide sequence