Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1214 and Pf6N2E2_1215 are separated by 168 nucleotides Pf6N2E2_1215 and Pf6N2E2_1216 are separated by 51 nucleotides Pf6N2E2_1216 and Pf6N2E2_1217 overlap by 4 nucleotides
Pf6N2E2_1214 - C-terminal domain of CinA type S; Protein Implicated in DNA repair function with RecA and MutS, at 1,419,804 to 1,420,304
_1214
Pf6N2E2_1215 - probable membrane protein YPO2362, at 1,420,473 to 1,421,084
_1215
Pf6N2E2_1216 - ATP-dependent DNA ligase (EC 6.5.1.1) clustered with Ku protein, LigD, at 1,421,136 to 1,423,772
_1216
Pf6N2E2_1217 - Gll2069 protein, at 1,423,769 to 1,424,920
_1217
Position (kb)
1421
1422
1423
1424 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1420.157 kb on - strand, within Pf6N2E2_1214 at 1420.276 kb on + strand at 1420.277 kb on - strand at 1420.277 kb on - strand at 1420.277 kb on - strand at 1420.528 kb on + strand at 1420.528 kb on + strand at 1420.528 kb on + strand at 1420.529 kb on - strand at 1420.529 kb on - strand at 1420.529 kb on - strand at 1420.529 kb on - strand at 1420.560 kb on + strand, within Pf6N2E2_1215 at 1420.601 kb on - strand, within Pf6N2E2_1215 at 1420.702 kb on - strand, within Pf6N2E2_1215 at 1420.702 kb on - strand, within Pf6N2E2_1215 at 1420.810 kb on - strand, within Pf6N2E2_1215 at 1421.046 kb on + strand at 1421.181 kb on - strand at 1421.383 kb on + strand at 1421.578 kb on - strand, within Pf6N2E2_1216 at 1421.938 kb on + strand, within Pf6N2E2_1216 at 1421.938 kb on + strand, within Pf6N2E2_1216 at 1421.939 kb on - strand, within Pf6N2E2_1216 at 1422.361 kb on + strand, within Pf6N2E2_1216 at 1422.361 kb on + strand, within Pf6N2E2_1216 at 1422.565 kb on + strand, within Pf6N2E2_1216 at 1422.565 kb on + strand, within Pf6N2E2_1216 at 1422.565 kb on + strand, within Pf6N2E2_1216 at 1422.565 kb on + strand, within Pf6N2E2_1216 at 1422.565 kb on + strand, within Pf6N2E2_1216 at 1422.565 kb on + strand, within Pf6N2E2_1216 at 1422.565 kb on + strand, within Pf6N2E2_1216 at 1422.565 kb on + strand, within Pf6N2E2_1216 at 1422.566 kb on - strand, within Pf6N2E2_1216 at 1422.566 kb on - strand, within Pf6N2E2_1216 at 1422.566 kb on - strand, within Pf6N2E2_1216 at 1422.566 kb on - strand, within Pf6N2E2_1216 at 1422.566 kb on - strand, within Pf6N2E2_1216 at 1422.757 kb on + strand, within Pf6N2E2_1216 at 1422.775 kb on + strand, within Pf6N2E2_1216 at 1422.775 kb on + strand, within Pf6N2E2_1216 at 1422.775 kb on + strand, within Pf6N2E2_1216 at 1422.776 kb on - strand, within Pf6N2E2_1216 at 1423.021 kb on + strand, within Pf6N2E2_1216 at 1423.022 kb on - strand, within Pf6N2E2_1216 at 1423.022 kb on - strand, within Pf6N2E2_1216 at 1423.022 kb on - strand, within Pf6N2E2_1216 at 1423.258 kb on + strand, within Pf6N2E2_1216 at 1423.507 kb on + strand, within Pf6N2E2_1216 at 1423.747 kb on + strand at 1423.747 kb on + strand at 1423.747 kb on + strand at 1423.748 kb on - strand at 1423.748 kb on - strand at 1423.748 kb on - strand at 1423.748 kb on - strand at 1423.942 kb on + strand, within Pf6N2E2_1217 at 1423.943 kb on - strand, within Pf6N2E2_1217 at 1423.961 kb on + strand, within Pf6N2E2_1217 at 1424.136 kb on + strand, within Pf6N2E2_1217 at 1424.137 kb on - strand, within Pf6N2E2_1217 at 1424.137 kb on - strand, within Pf6N2E2_1217 at 1424.277 kb on - strand, within Pf6N2E2_1217 at 1424.337 kb on + strand, within Pf6N2E2_1217 at 1424.433 kb on + strand, within Pf6N2E2_1217 at 1424.433 kb on + strand, within Pf6N2E2_1217 at 1424.549 kb on - strand, within Pf6N2E2_1217 at 1424.590 kb on - strand, within Pf6N2E2_1217 at 1424.590 kb on - strand, within Pf6N2E2_1217 at 1424.590 kb on - strand, within Pf6N2E2_1217
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 1,420,157 - Pf6N2E2_1214 0.70 +0.2 1,420,276 + +0.6 1,420,277 - +0.7 1,420,277 - +0.9 1,420,277 - -0.1 1,420,528 + -0.8 1,420,528 + -0.6 1,420,528 + +0.2 1,420,529 - +1.1 1,420,529 - +0.5 1,420,529 - +0.2 1,420,529 - -0.2 1,420,560 + Pf6N2E2_1215 0.14 +0.4 1,420,601 - Pf6N2E2_1215 0.21 +0.3 1,420,702 - Pf6N2E2_1215 0.37 +0.3 1,420,702 - Pf6N2E2_1215 0.37 +0.1 1,420,810 - Pf6N2E2_1215 0.55 -0.3 1,421,046 + -0.9 1,421,181 - -0.3 1,421,383 + -0.3 1,421,578 - Pf6N2E2_1216 0.17 -0.6 1,421,938 + Pf6N2E2_1216 0.30 +0.7 1,421,938 + Pf6N2E2_1216 0.30 +0.7 1,421,939 - Pf6N2E2_1216 0.30 -0.0 1,422,361 + Pf6N2E2_1216 0.46 +1.0 1,422,361 + Pf6N2E2_1216 0.46 +0.6 1,422,565 + Pf6N2E2_1216 0.54 +0.8 1,422,565 + Pf6N2E2_1216 0.54 +1.0 1,422,565 + Pf6N2E2_1216 0.54 +1.0 1,422,565 + Pf6N2E2_1216 0.54 +0.0 1,422,565 + Pf6N2E2_1216 0.54 -0.4 1,422,565 + Pf6N2E2_1216 0.54 +0.9 1,422,565 + Pf6N2E2_1216 0.54 +0.5 1,422,565 + Pf6N2E2_1216 0.54 +0.2 1,422,566 - Pf6N2E2_1216 0.54 +0.9 1,422,566 - Pf6N2E2_1216 0.54 -0.3 1,422,566 - Pf6N2E2_1216 0.54 -1.9 1,422,566 - Pf6N2E2_1216 0.54 +0.2 1,422,566 - Pf6N2E2_1216 0.54 +1.3 1,422,757 + Pf6N2E2_1216 0.61 +0.7 1,422,775 + Pf6N2E2_1216 0.62 -3.0 1,422,775 + Pf6N2E2_1216 0.62 +1.1 1,422,775 + Pf6N2E2_1216 0.62 -0.2 1,422,776 - Pf6N2E2_1216 0.62 +1.0 1,423,021 + Pf6N2E2_1216 0.71 -0.2 1,423,022 - Pf6N2E2_1216 0.72 +0.3 1,423,022 - Pf6N2E2_1216 0.72 -0.4 1,423,022 - Pf6N2E2_1216 0.72 -0.1 1,423,258 + Pf6N2E2_1216 0.80 -0.2 1,423,507 + Pf6N2E2_1216 0.90 +1.3 1,423,747 + +0.0 1,423,747 + +0.6 1,423,747 + -1.3 1,423,748 - -0.0 1,423,748 - +0.2 1,423,748 - +0.1 1,423,748 - -0.5 1,423,942 + Pf6N2E2_1217 0.15 -0.7 1,423,943 - Pf6N2E2_1217 0.15 -0.3 1,423,961 + Pf6N2E2_1217 0.17 +0.3 1,424,136 + Pf6N2E2_1217 0.32 +0.1 1,424,137 - Pf6N2E2_1217 0.32 +0.4 1,424,137 - Pf6N2E2_1217 0.32 -1.6 1,424,277 - Pf6N2E2_1217 0.44 -0.5 1,424,337 + Pf6N2E2_1217 0.49 -0.5 1,424,433 + Pf6N2E2_1217 0.58 -1.5 1,424,433 + Pf6N2E2_1217 0.58 +0.5 1,424,549 - Pf6N2E2_1217 0.68 -1.0 1,424,590 - Pf6N2E2_1217 0.71 +0.2 1,424,590 - Pf6N2E2_1217 0.71 -0.5 1,424,590 - Pf6N2E2_1217 0.71 +0.9
Or see this region's nucleotide sequence