Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_109 and Pf6N2E2_110 are separated by 188 nucleotides Pf6N2E2_110 and Pf6N2E2_111 overlap by 17 nucleotides Pf6N2E2_111 and Pf6N2E2_112 are separated by 5 nucleotides Pf6N2E2_112 and Pf6N2E2_113 overlap by 4 nucleotides
Pf6N2E2_109 - Hydrolases of the alpha/beta superfamily, at 154,615 to 155,490
_109
Pf6N2E2_110 - Cellobiose phosphotransferase system YdjC-like protein, at 155,679 to 156,461
_110
Pf6N2E2_111 - FIG00954746: hypothetical protein, at 156,445 to 157,620
_111
Pf6N2E2_112 - hypothetical protein, at 157,626 to 158,315
_112
Pf6N2E2_113 - FIG00963512: hypothetical protein, at 158,312 to 160,225
_113
Position (kb) 156
157
158 Strain fitness (log2 ratio) -2
-1
0
1 at 155.448 kb on + strand at 155.448 kb on + strand at 155.448 kb on + strand at 155.448 kb on + strand at 155.448 kb on + strand at 155.448 kb on + strand at 155.449 kb on - strand at 155.449 kb on - strand at 155.449 kb on - strand at 155.449 kb on - strand at 155.545 kb on + strand at 155.546 kb on - strand at 155.546 kb on - strand at 155.591 kb on - strand at 155.640 kb on + strand at 155.662 kb on + strand at 155.769 kb on + strand, within Pf6N2E2_110 at 155.769 kb on + strand, within Pf6N2E2_110 at 155.770 kb on - strand, within Pf6N2E2_110 at 155.860 kb on + strand, within Pf6N2E2_110 at 155.860 kb on + strand, within Pf6N2E2_110 at 155.861 kb on - strand, within Pf6N2E2_110 at 155.861 kb on - strand, within Pf6N2E2_110 at 155.861 kb on - strand, within Pf6N2E2_110 at 155.905 kb on + strand, within Pf6N2E2_110 at 155.906 kb on - strand, within Pf6N2E2_110 at 155.906 kb on - strand, within Pf6N2E2_110 at 156.009 kb on + strand, within Pf6N2E2_110 at 156.009 kb on + strand, within Pf6N2E2_110 at 156.010 kb on - strand, within Pf6N2E2_110 at 156.274 kb on + strand, within Pf6N2E2_110 at 156.374 kb on + strand, within Pf6N2E2_110 at 156.658 kb on + strand, within Pf6N2E2_111 at 156.659 kb on - strand, within Pf6N2E2_111 at 156.800 kb on + strand, within Pf6N2E2_111 at 156.800 kb on + strand, within Pf6N2E2_111 at 156.800 kb on + strand, within Pf6N2E2_111 at 156.800 kb on + strand, within Pf6N2E2_111 at 156.800 kb on + strand, within Pf6N2E2_111 at 156.806 kb on + strand, within Pf6N2E2_111 at 156.806 kb on + strand, within Pf6N2E2_111 at 156.806 kb on + strand, within Pf6N2E2_111 at 156.806 kb on + strand, within Pf6N2E2_111 at 156.807 kb on - strand, within Pf6N2E2_111 at 156.807 kb on - strand, within Pf6N2E2_111 at 156.827 kb on - strand, within Pf6N2E2_111 at 157.148 kb on - strand, within Pf6N2E2_111 at 157.462 kb on + strand, within Pf6N2E2_111 at 157.462 kb on + strand, within Pf6N2E2_111 at 157.462 kb on + strand, within Pf6N2E2_111 at 157.462 kb on + strand, within Pf6N2E2_111 at 157.463 kb on - strand, within Pf6N2E2_111 at 157.559 kb on + strand at 157.664 kb on + strand at 157.665 kb on - strand at 157.694 kb on - strand at 157.736 kb on - strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.778 kb on + strand, within Pf6N2E2_112 at 157.778 kb on + strand, within Pf6N2E2_112 at 157.779 kb on - strand, within Pf6N2E2_112 at 157.806 kb on + strand, within Pf6N2E2_112 at 157.806 kb on + strand, within Pf6N2E2_112 at 157.806 kb on + strand, within Pf6N2E2_112 at 157.807 kb on - strand, within Pf6N2E2_112 at 157.934 kb on - strand, within Pf6N2E2_112 at 157.984 kb on + strand, within Pf6N2E2_112 at 158.038 kb on + strand, within Pf6N2E2_112 at 158.038 kb on + strand, within Pf6N2E2_112 at 158.039 kb on - strand, within Pf6N2E2_112 at 158.053 kb on + strand, within Pf6N2E2_112 at 158.171 kb on + strand, within Pf6N2E2_112 at 158.212 kb on - strand, within Pf6N2E2_112 at 158.212 kb on - strand, within Pf6N2E2_112 at 158.236 kb on + strand, within Pf6N2E2_112 at 158.237 kb on - strand, within Pf6N2E2_112 at 158.461 kb on - strand at 158.576 kb on + strand, within Pf6N2E2_113 at 158.576 kb on + strand, within Pf6N2E2_113 at 158.577 kb on - strand, within Pf6N2E2_113
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 155,448 + +0.8 155,448 + -0.5 155,448 + -0.5 155,448 + +0.0 155,448 + -1.0 155,448 + +0.2 155,449 - +1.2 155,449 - -0.2 155,449 - +0.1 155,449 - +1.0 155,545 + -0.2 155,546 - -0.6 155,546 - -2.0 155,591 - -0.6 155,640 + +0.8 155,662 + -0.2 155,769 + Pf6N2E2_110 0.11 -0.1 155,769 + Pf6N2E2_110 0.11 -0.4 155,770 - Pf6N2E2_110 0.12 +0.3 155,860 + Pf6N2E2_110 0.23 +0.3 155,860 + Pf6N2E2_110 0.23 +1.0 155,861 - Pf6N2E2_110 0.23 -0.6 155,861 - Pf6N2E2_110 0.23 -0.7 155,861 - Pf6N2E2_110 0.23 -0.1 155,905 + Pf6N2E2_110 0.29 +0.1 155,906 - Pf6N2E2_110 0.29 -0.5 155,906 - Pf6N2E2_110 0.29 +0.8 156,009 + Pf6N2E2_110 0.42 -0.1 156,009 + Pf6N2E2_110 0.42 +0.5 156,010 - Pf6N2E2_110 0.42 +0.2 156,274 + Pf6N2E2_110 0.76 -0.1 156,374 + Pf6N2E2_110 0.89 -0.2 156,658 + Pf6N2E2_111 0.18 +0.2 156,659 - Pf6N2E2_111 0.18 -0.4 156,800 + Pf6N2E2_111 0.30 -1.2 156,800 + Pf6N2E2_111 0.30 -1.3 156,800 + Pf6N2E2_111 0.30 +0.0 156,800 + Pf6N2E2_111 0.30 +0.4 156,800 + Pf6N2E2_111 0.30 +0.5 156,806 + Pf6N2E2_111 0.31 -0.4 156,806 + Pf6N2E2_111 0.31 -0.8 156,806 + Pf6N2E2_111 0.31 +0.7 156,806 + Pf6N2E2_111 0.31 +0.0 156,807 - Pf6N2E2_111 0.31 -0.9 156,807 - Pf6N2E2_111 0.31 +0.4 156,827 - Pf6N2E2_111 0.32 -2.2 157,148 - Pf6N2E2_111 0.60 +0.1 157,462 + Pf6N2E2_111 0.86 -0.9 157,462 + Pf6N2E2_111 0.86 -1.3 157,462 + Pf6N2E2_111 0.86 -1.6 157,462 + Pf6N2E2_111 0.86 +0.4 157,463 - Pf6N2E2_111 0.87 +0.0 157,559 + +0.7 157,664 + -0.8 157,665 - +0.1 157,694 - -0.0 157,736 - Pf6N2E2_112 0.16 -0.8 157,776 + Pf6N2E2_112 0.22 +1.1 157,776 + Pf6N2E2_112 0.22 -2.1 157,776 + Pf6N2E2_112 0.22 +1.3 157,776 + Pf6N2E2_112 0.22 +0.4 157,776 + Pf6N2E2_112 0.22 +0.5 157,776 + Pf6N2E2_112 0.22 +0.6 157,776 + Pf6N2E2_112 0.22 +0.2 157,776 + Pf6N2E2_112 0.22 +0.2 157,777 - Pf6N2E2_112 0.22 +0.8 157,777 - Pf6N2E2_112 0.22 -0.6 157,777 - Pf6N2E2_112 0.22 -0.4 157,777 - Pf6N2E2_112 0.22 -0.3 157,777 - Pf6N2E2_112 0.22 -0.3 157,777 - Pf6N2E2_112 0.22 -0.0 157,777 - Pf6N2E2_112 0.22 -0.5 157,777 - Pf6N2E2_112 0.22 +0.8 157,778 + Pf6N2E2_112 0.22 -1.1 157,778 + Pf6N2E2_112 0.22 -0.8 157,779 - Pf6N2E2_112 0.22 -0.1 157,806 + Pf6N2E2_112 0.26 +0.5 157,806 + Pf6N2E2_112 0.26 -0.1 157,806 + Pf6N2E2_112 0.26 -0.1 157,807 - Pf6N2E2_112 0.26 -0.0 157,934 - Pf6N2E2_112 0.45 +0.2 157,984 + Pf6N2E2_112 0.52 +1.1 158,038 + Pf6N2E2_112 0.60 -0.0 158,038 + Pf6N2E2_112 0.60 +0.5 158,039 - Pf6N2E2_112 0.60 +0.3 158,053 + Pf6N2E2_112 0.62 +0.1 158,171 + Pf6N2E2_112 0.79 -0.8 158,212 - Pf6N2E2_112 0.85 -0.0 158,212 - Pf6N2E2_112 0.85 +0.3 158,236 + Pf6N2E2_112 0.88 -0.2 158,237 - Pf6N2E2_112 0.89 -1.3 158,461 - -0.1 158,576 + Pf6N2E2_113 0.14 -0.6 158,576 + Pf6N2E2_113 0.14 +0.3 158,577 - Pf6N2E2_113 0.14 -1.0
Or see this region's nucleotide sequence