Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1076 and Pf6N2E2_1077 overlap by 4 nucleotides Pf6N2E2_1077 and Pf6N2E2_1078 are separated by 49 nucleotides
Pf6N2E2_1076 - 4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25), at 1,257,423 to 1,259,501
_1076
Pf6N2E2_1077 - Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141), at 1,259,498 to 1,261,258
_1077
Pf6N2E2_1078 - Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21), at 1,261,308 to 1,262,879
_1078
Position (kb)
1259
1260
1261
1262 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1258.874 kb on - strand, within Pf6N2E2_1076 at 1258.874 kb on - strand, within Pf6N2E2_1076 at 1259.023 kb on + strand, within Pf6N2E2_1076 at 1259.023 kb on + strand, within Pf6N2E2_1076 at 1259.024 kb on - strand, within Pf6N2E2_1076 at 1259.024 kb on - strand, within Pf6N2E2_1076 at 1259.024 kb on - strand, within Pf6N2E2_1076 at 1259.087 kb on - strand, within Pf6N2E2_1076 at 1259.251 kb on + strand, within Pf6N2E2_1076 at 1259.252 kb on - strand, within Pf6N2E2_1076 at 1259.252 kb on - strand, within Pf6N2E2_1076 at 1260.005 kb on - strand, within Pf6N2E2_1077 at 1260.071 kb on + strand, within Pf6N2E2_1077 at 1260.307 kb on - strand, within Pf6N2E2_1077 at 1260.315 kb on + strand, within Pf6N2E2_1077 at 1260.315 kb on + strand, within Pf6N2E2_1077 at 1260.318 kb on + strand, within Pf6N2E2_1077 at 1260.319 kb on - strand, within Pf6N2E2_1077 at 1260.387 kb on + strand, within Pf6N2E2_1077 at 1260.387 kb on + strand, within Pf6N2E2_1077 at 1260.388 kb on - strand, within Pf6N2E2_1077 at 1260.519 kb on - strand, within Pf6N2E2_1077 at 1260.549 kb on + strand, within Pf6N2E2_1077 at 1260.550 kb on - strand, within Pf6N2E2_1077 at 1260.678 kb on + strand, within Pf6N2E2_1077 at 1260.678 kb on + strand, within Pf6N2E2_1077 at 1260.678 kb on + strand, within Pf6N2E2_1077 at 1260.678 kb on + strand, within Pf6N2E2_1077 at 1260.678 kb on + strand, within Pf6N2E2_1077 at 1260.678 kb on + strand, within Pf6N2E2_1077 at 1260.705 kb on + strand, within Pf6N2E2_1077 at 1260.705 kb on + strand, within Pf6N2E2_1077 at 1260.705 kb on + strand, within Pf6N2E2_1077 at 1260.705 kb on + strand, within Pf6N2E2_1077 at 1260.706 kb on - strand, within Pf6N2E2_1077 at 1261.005 kb on + strand, within Pf6N2E2_1077 at 1261.101 kb on + strand at 1261.102 kb on - strand at 1261.304 kb on + strand at 1261.305 kb on - strand at 1261.337 kb on + strand at 1261.337 kb on + strand at 1261.355 kb on - strand at 1261.355 kb on - strand at 1261.366 kb on + strand at 1261.366 kb on + strand at 1261.481 kb on - strand, within Pf6N2E2_1078 at 1261.481 kb on - strand, within Pf6N2E2_1078 at 1261.591 kb on + strand, within Pf6N2E2_1078 at 1261.592 kb on - strand, within Pf6N2E2_1078 at 1261.592 kb on - strand, within Pf6N2E2_1078 at 1262.095 kb on + strand, within Pf6N2E2_1078 at 1262.095 kb on + strand, within Pf6N2E2_1078 at 1262.095 kb on + strand, within Pf6N2E2_1078 at 1262.095 kb on + strand, within Pf6N2E2_1078 at 1262.095 kb on + strand, within Pf6N2E2_1078 at 1262.096 kb on - strand, within Pf6N2E2_1078 at 1262.096 kb on - strand, within Pf6N2E2_1078 at 1262.096 kb on - strand, within Pf6N2E2_1078 at 1262.117 kb on + strand, within Pf6N2E2_1078 at 1262.117 kb on + strand, within Pf6N2E2_1078 at 1262.117 kb on + strand, within Pf6N2E2_1078 at 1262.128 kb on + strand, within Pf6N2E2_1078 at 1262.128 kb on + strand, within Pf6N2E2_1078 at 1262.129 kb on - strand, within Pf6N2E2_1078 at 1262.129 kb on - strand, within Pf6N2E2_1078 at 1262.165 kb on - strand, within Pf6N2E2_1078
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 1,258,874 - Pf6N2E2_1076 0.70 +0.6 1,258,874 - Pf6N2E2_1076 0.70 +0.1 1,259,023 + Pf6N2E2_1076 0.77 +0.7 1,259,023 + Pf6N2E2_1076 0.77 +0.6 1,259,024 - Pf6N2E2_1076 0.77 -0.1 1,259,024 - Pf6N2E2_1076 0.77 -0.9 1,259,024 - Pf6N2E2_1076 0.77 +0.5 1,259,087 - Pf6N2E2_1076 0.80 -2.1 1,259,251 + Pf6N2E2_1076 0.88 +0.8 1,259,252 - Pf6N2E2_1076 0.88 +0.7 1,259,252 - Pf6N2E2_1076 0.88 -0.1 1,260,005 - Pf6N2E2_1077 0.29 -0.3 1,260,071 + Pf6N2E2_1077 0.33 -0.3 1,260,307 - Pf6N2E2_1077 0.46 -0.8 1,260,315 + Pf6N2E2_1077 0.46 +0.4 1,260,315 + Pf6N2E2_1077 0.46 -0.9 1,260,318 + Pf6N2E2_1077 0.47 -2.1 1,260,319 - Pf6N2E2_1077 0.47 -2.6 1,260,387 + Pf6N2E2_1077 0.50 +1.3 1,260,387 + Pf6N2E2_1077 0.50 -0.5 1,260,388 - Pf6N2E2_1077 0.51 -0.6 1,260,519 - Pf6N2E2_1077 0.58 -1.9 1,260,549 + Pf6N2E2_1077 0.60 -0.6 1,260,550 - Pf6N2E2_1077 0.60 +0.8 1,260,678 + Pf6N2E2_1077 0.67 -0.6 1,260,678 + Pf6N2E2_1077 0.67 -0.7 1,260,678 + Pf6N2E2_1077 0.67 +0.4 1,260,678 + Pf6N2E2_1077 0.67 -1.0 1,260,678 + Pf6N2E2_1077 0.67 -1.9 1,260,678 + Pf6N2E2_1077 0.67 -0.2 1,260,705 + Pf6N2E2_1077 0.69 -0.2 1,260,705 + Pf6N2E2_1077 0.69 +0.7 1,260,705 + Pf6N2E2_1077 0.69 -0.8 1,260,705 + Pf6N2E2_1077 0.69 +0.2 1,260,706 - Pf6N2E2_1077 0.69 -1.6 1,261,005 + Pf6N2E2_1077 0.86 +0.3 1,261,101 + -0.4 1,261,102 - +0.6 1,261,304 + +0.5 1,261,305 - +0.0 1,261,337 + +0.6 1,261,337 + -1.3 1,261,355 - +0.1 1,261,355 - +1.2 1,261,366 + -0.2 1,261,366 + +0.5 1,261,481 - Pf6N2E2_1078 0.11 -0.4 1,261,481 - Pf6N2E2_1078 0.11 -0.2 1,261,591 + Pf6N2E2_1078 0.18 -0.3 1,261,592 - Pf6N2E2_1078 0.18 -0.2 1,261,592 - Pf6N2E2_1078 0.18 +2.0 1,262,095 + Pf6N2E2_1078 0.50 +0.0 1,262,095 + Pf6N2E2_1078 0.50 -0.2 1,262,095 + Pf6N2E2_1078 0.50 +0.8 1,262,095 + Pf6N2E2_1078 0.50 -0.9 1,262,095 + Pf6N2E2_1078 0.50 +1.0 1,262,096 - Pf6N2E2_1078 0.50 -0.4 1,262,096 - Pf6N2E2_1078 0.50 +0.7 1,262,096 - Pf6N2E2_1078 0.50 +0.4 1,262,117 + Pf6N2E2_1078 0.51 -0.6 1,262,117 + Pf6N2E2_1078 0.51 +1.0 1,262,117 + Pf6N2E2_1078 0.51 -0.8 1,262,128 + Pf6N2E2_1078 0.52 +1.1 1,262,128 + Pf6N2E2_1078 0.52 -2.2 1,262,129 - Pf6N2E2_1078 0.52 +0.8 1,262,129 - Pf6N2E2_1078 0.52 -0.1 1,262,165 - Pf6N2E2_1078 0.55 +1.0
Or see this region's nucleotide sequence