Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07245 and MMJJ_RS07250 are separated by 73 nucleotides MMJJ_RS07250 and MMJJ_RS09365 are separated by 20 nucleotides MMJJ_RS09365 and MMJJ_RS07255 are separated by 204 nucleotides
MMJJ_RS07245: MMJJ_RS07245 - amidohydrolase family protein, at 1,327,152 to 1,328,270
_RS07245
MMJJ_RS07250: MMJJ_RS07250 - thymidylate synthase, at 1,328,344 to 1,328,976
_RS07250
MMJJ_RS09365: MMJJ_RS09365 - hypothetical protein, at 1,328,997 to 1,329,188
_RS09365
MMJJ_RS07255: MMJJ_RS07255 - hypothetical protein, at 1,329,393 to 1,330,451
_RS07255
Position (kb)
1328
1329
1330 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1328.009 kb on - strand, within MMJJ_RS07245 at 1328.013 kb on - strand, within MMJJ_RS07245 at 1328.079 kb on + strand, within MMJJ_RS07245 at 1328.088 kb on + strand, within MMJJ_RS07245 at 1328.121 kb on + strand at 1328.243 kb on + strand at 1328.245 kb on + strand at 1328.289 kb on + strand at 1328.392 kb on + strand at 1328.463 kb on - strand, within MMJJ_RS07250 at 1328.763 kb on + strand, within MMJJ_RS07250 at 1328.975 kb on + strand at 1328.980 kb on - strand at 1329.003 kb on + strand at 1329.012 kb on - strand at 1329.022 kb on + strand, within MMJJ_RS09365 at 1329.040 kb on + strand, within MMJJ_RS09365 at 1329.071 kb on + strand, within MMJJ_RS09365 at 1329.145 kb on + strand, within MMJJ_RS09365 at 1329.176 kb on - strand at 1329.181 kb on + strand at 1329.200 kb on + strand at 1329.209 kb on - strand at 1329.225 kb on + strand at 1329.242 kb on + strand at 1329.244 kb on - strand at 1329.244 kb on - strand at 1329.244 kb on - strand at 1329.245 kb on + strand at 1329.245 kb on + strand at 1329.255 kb on - strand at 1329.255 kb on - strand at 1329.255 kb on - strand at 1329.256 kb on + strand at 1329.260 kb on + strand at 1329.270 kb on - strand at 1329.271 kb on + strand at 1329.271 kb on + strand at 1329.273 kb on - strand at 1329.273 kb on - strand at 1329.274 kb on + strand at 1329.274 kb on + strand at 1329.275 kb on - strand at 1329.275 kb on - strand at 1329.276 kb on + strand at 1329.276 kb on + strand at 1329.284 kb on - strand at 1329.288 kb on + strand at 1329.292 kb on - strand at 1329.307 kb on + strand at 1329.307 kb on + strand at 1329.312 kb on - strand at 1329.317 kb on - strand at 1329.317 kb on - strand at 1329.318 kb on + strand at 1329.323 kb on + strand at 1329.323 kb on + strand at 1329.323 kb on + strand at 1329.323 kb on + strand at 1329.323 kb on + strand at 1329.356 kb on + strand at 1329.357 kb on - strand at 1329.358 kb on + strand at 1329.476 kb on - strand at 1329.476 kb on - strand at 1329.481 kb on - strand at 1329.482 kb on + strand at 1329.482 kb on + strand at 1329.485 kb on - strand at 1329.486 kb on + strand at 1329.511 kb on - strand, within MMJJ_RS07255 at 1329.616 kb on - strand, within MMJJ_RS07255 at 1329.630 kb on - strand, within MMJJ_RS07255 at 1329.630 kb on - strand, within MMJJ_RS07255 at 1329.630 kb on - strand, within MMJJ_RS07255 at 1329.631 kb on + strand, within MMJJ_RS07255 at 1329.634 kb on - strand, within MMJJ_RS07255 at 1329.634 kb on - strand, within MMJJ_RS07255 at 1329.635 kb on + strand, within MMJJ_RS07255 at 1329.639 kb on - strand, within MMJJ_RS07255 at 1329.639 kb on - strand, within MMJJ_RS07255 at 1329.639 kb on - strand, within MMJJ_RS07255 at 1329.639 kb on - strand, within MMJJ_RS07255 at 1329.639 kb on - strand, within MMJJ_RS07255 at 1329.639 kb on - strand, within MMJJ_RS07255 at 1329.639 kb on - strand, within MMJJ_RS07255 at 1329.639 kb on - strand, within MMJJ_RS07255 at 1329.639 kb on - strand, within MMJJ_RS07255 at 1329.639 kb on - strand, within MMJJ_RS07255 at 1329.640 kb on + strand, within MMJJ_RS07255 at 1329.640 kb on + strand, within MMJJ_RS07255 at 1329.647 kb on + strand, within MMJJ_RS07255 at 1329.658 kb on + strand, within MMJJ_RS07255 at 1329.722 kb on - strand, within MMJJ_RS07255 at 1329.764 kb on - strand, within MMJJ_RS07255 at 1329.787 kb on + strand, within MMJJ_RS07255 at 1329.787 kb on + strand, within MMJJ_RS07255 at 1329.809 kb on - strand, within MMJJ_RS07255 at 1329.809 kb on - strand, within MMJJ_RS07255 at 1329.810 kb on + strand, within MMJJ_RS07255 at 1329.821 kb on - strand, within MMJJ_RS07255 at 1329.821 kb on - strand, within MMJJ_RS07255 at 1329.821 kb on - strand, within MMJJ_RS07255 at 1329.824 kb on - strand, within MMJJ_RS07255 at 1329.825 kb on + strand, within MMJJ_RS07255 at 1329.825 kb on + strand, within MMJJ_RS07255 at 1329.845 kb on - strand, within MMJJ_RS07255 at 1329.845 kb on - strand, within MMJJ_RS07255 at 1329.847 kb on - strand, within MMJJ_RS07255 at 1329.847 kb on - strand, within MMJJ_RS07255 at 1329.848 kb on + strand, within MMJJ_RS07255 at 1329.852 kb on - strand, within MMJJ_RS07255 at 1329.852 kb on - strand, within MMJJ_RS07255 at 1329.852 kb on - strand, within MMJJ_RS07255 at 1329.853 kb on + strand, within MMJJ_RS07255 at 1329.856 kb on - strand, within MMJJ_RS07255 at 1329.856 kb on - strand, within MMJJ_RS07255 at 1329.857 kb on + strand, within MMJJ_RS07255 at 1329.857 kb on + strand, within MMJJ_RS07255 at 1329.857 kb on + strand, within MMJJ_RS07255 at 1329.857 kb on + strand, within MMJJ_RS07255 at 1329.857 kb on + strand, within MMJJ_RS07255 at 1329.864 kb on - strand, within MMJJ_RS07255 at 1329.898 kb on - strand, within MMJJ_RS07255 at 1329.898 kb on - strand, within MMJJ_RS07255 at 1329.899 kb on + strand, within MMJJ_RS07255 at 1329.899 kb on + strand, within MMJJ_RS07255 at 1329.901 kb on - strand, within MMJJ_RS07255 at 1329.912 kb on - strand, within MMJJ_RS07255 at 1329.913 kb on + strand, within MMJJ_RS07255 at 1329.913 kb on + strand, within MMJJ_RS07255 at 1329.968 kb on - strand, within MMJJ_RS07255 at 1329.968 kb on - strand, within MMJJ_RS07255 at 1329.969 kb on + strand, within MMJJ_RS07255 at 1329.970 kb on - strand, within MMJJ_RS07255 at 1329.970 kb on - strand, within MMJJ_RS07255 at 1329.971 kb on + strand, within MMJJ_RS07255 at 1329.971 kb on + strand, within MMJJ_RS07255 at 1330.021 kb on - strand, within MMJJ_RS07255 at 1330.022 kb on + strand, within MMJJ_RS07255 at 1330.076 kb on - strand, within MMJJ_RS07255 at 1330.076 kb on - strand, within MMJJ_RS07255 at 1330.076 kb on - strand, within MMJJ_RS07255 at 1330.076 kb on - strand, within MMJJ_RS07255 at 1330.076 kb on - strand, within MMJJ_RS07255 at 1330.076 kb on - strand, within MMJJ_RS07255 at 1330.077 kb on + strand, within MMJJ_RS07255 at 1330.077 kb on + strand, within MMJJ_RS07255 at 1330.079 kb on - strand, within MMJJ_RS07255 at 1330.080 kb on + strand, within MMJJ_RS07255 at 1330.080 kb on + strand, within MMJJ_RS07255 at 1330.082 kb on - strand, within MMJJ_RS07255 at 1330.082 kb on - strand, within MMJJ_RS07255 at 1330.082 kb on - strand, within MMJJ_RS07255 at 1330.083 kb on + strand, within MMJJ_RS07255 at 1330.083 kb on + strand, within MMJJ_RS07255 at 1330.083 kb on + strand, within MMJJ_RS07255 at 1330.084 kb on - strand, within MMJJ_RS07255 at 1330.121 kb on + strand, within MMJJ_RS07255 at 1330.121 kb on + strand, within MMJJ_RS07255 at 1330.124 kb on - strand, within MMJJ_RS07255 at 1330.124 kb on - strand, within MMJJ_RS07255 at 1330.125 kb on + strand, within MMJJ_RS07255 at 1330.162 kb on - strand, within MMJJ_RS07255 at 1330.162 kb on - strand, within MMJJ_RS07255 at 1330.162 kb on - strand, within MMJJ_RS07255 at 1330.169 kb on - strand, within MMJJ_RS07255 at 1330.170 kb on + strand, within MMJJ_RS07255 at 1330.172 kb on - strand, within MMJJ_RS07255 at 1330.172 kb on - strand, within MMJJ_RS07255 at 1330.173 kb on + strand, within MMJJ_RS07255 at 1330.173 kb on + strand, within MMJJ_RS07255 at 1330.173 kb on + strand, within MMJJ_RS07255
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,328,009 - MMJJ_RS07245 0.77 -1.1 1,328,013 - MMJJ_RS07245 0.77 -1.6 1,328,079 + MMJJ_RS07245 0.83 -0.1 1,328,088 + MMJJ_RS07245 0.84 -1.4 1,328,121 + -0.1 1,328,243 + -3.1 1,328,245 + -2.4 1,328,289 + +1.2 1,328,392 + +1.5 1,328,463 - MMJJ_RS07250 0.19 -1.7 1,328,763 + MMJJ_RS07250 0.66 +0.3 1,328,975 + +0.5 1,328,980 - -0.5 1,329,003 + -1.5 1,329,012 - +0.1 1,329,022 + MMJJ_RS09365 0.13 +1.8 1,329,040 + MMJJ_RS09365 0.22 -0.7 1,329,071 + MMJJ_RS09365 0.39 +0.7 1,329,145 + MMJJ_RS09365 0.77 +1.2 1,329,176 - +1.7 1,329,181 + -0.1 1,329,200 + +0.1 1,329,209 - +0.6 1,329,225 + +0.3 1,329,242 + -0.3 1,329,244 - +1.3 1,329,244 - -0.8 1,329,244 - -2.1 1,329,245 + -2.0 1,329,245 + +0.9 1,329,255 - -0.2 1,329,255 - -0.3 1,329,255 - -0.3 1,329,256 + -0.5 1,329,260 + +1.4 1,329,270 - +0.7 1,329,271 + -0.9 1,329,271 + +0.8 1,329,273 - +1.2 1,329,273 - +0.3 1,329,274 + +1.7 1,329,274 + +0.2 1,329,275 - -0.4 1,329,275 - +0.8 1,329,276 + +0.9 1,329,276 + -1.5 1,329,284 - -0.4 1,329,288 + -0.7 1,329,292 - -0.9 1,329,307 + +0.6 1,329,307 + +1.0 1,329,312 - +0.6 1,329,317 - -0.1 1,329,317 - +0.8 1,329,318 + -0.5 1,329,323 + +1.2 1,329,323 + -0.3 1,329,323 + +1.0 1,329,323 + +0.5 1,329,323 + +0.3 1,329,356 + -0.0 1,329,357 - +1.2 1,329,358 + +0.6 1,329,476 - +0.1 1,329,476 - +1.2 1,329,481 - +1.0 1,329,482 + +0.2 1,329,482 + -0.5 1,329,485 - -0.1 1,329,486 + +0.4 1,329,511 - MMJJ_RS07255 0.11 +0.1 1,329,616 - MMJJ_RS07255 0.21 -0.1 1,329,630 - MMJJ_RS07255 0.22 -1.6 1,329,630 - MMJJ_RS07255 0.22 +0.4 1,329,630 - MMJJ_RS07255 0.22 +0.5 1,329,631 + MMJJ_RS07255 0.22 +0.5 1,329,634 - MMJJ_RS07255 0.23 -0.4 1,329,634 - MMJJ_RS07255 0.23 -0.6 1,329,635 + MMJJ_RS07255 0.23 -1.3 1,329,639 - MMJJ_RS07255 0.23 +1.8 1,329,639 - MMJJ_RS07255 0.23 +0.2 1,329,639 - MMJJ_RS07255 0.23 +1.9 1,329,639 - MMJJ_RS07255 0.23 -0.4 1,329,639 - MMJJ_RS07255 0.23 +0.3 1,329,639 - MMJJ_RS07255 0.23 +0.8 1,329,639 - MMJJ_RS07255 0.23 -1.1 1,329,639 - MMJJ_RS07255 0.23 -0.3 1,329,639 - MMJJ_RS07255 0.23 -0.7 1,329,639 - MMJJ_RS07255 0.23 +1.1 1,329,640 + MMJJ_RS07255 0.23 +1.4 1,329,640 + MMJJ_RS07255 0.23 -0.6 1,329,647 + MMJJ_RS07255 0.24 +0.9 1,329,658 + MMJJ_RS07255 0.25 +0.3 1,329,722 - MMJJ_RS07255 0.31 +0.6 1,329,764 - MMJJ_RS07255 0.35 -0.1 1,329,787 + MMJJ_RS07255 0.37 -0.0 1,329,787 + MMJJ_RS07255 0.37 -0.7 1,329,809 - MMJJ_RS07255 0.39 +0.3 1,329,809 - MMJJ_RS07255 0.39 +0.9 1,329,810 + MMJJ_RS07255 0.39 +1.1 1,329,821 - MMJJ_RS07255 0.40 +0.4 1,329,821 - MMJJ_RS07255 0.40 +0.8 1,329,821 - MMJJ_RS07255 0.40 -0.5 1,329,824 - MMJJ_RS07255 0.41 +0.6 1,329,825 + MMJJ_RS07255 0.41 +1.0 1,329,825 + MMJJ_RS07255 0.41 -0.4 1,329,845 - MMJJ_RS07255 0.43 +0.6 1,329,845 - MMJJ_RS07255 0.43 -0.9 1,329,847 - MMJJ_RS07255 0.43 +0.4 1,329,847 - MMJJ_RS07255 0.43 +0.4 1,329,848 + MMJJ_RS07255 0.43 +0.7 1,329,852 - MMJJ_RS07255 0.43 +0.4 1,329,852 - MMJJ_RS07255 0.43 +0.8 1,329,852 - MMJJ_RS07255 0.43 +0.1 1,329,853 + MMJJ_RS07255 0.43 +1.0 1,329,856 - MMJJ_RS07255 0.44 -0.4 1,329,856 - MMJJ_RS07255 0.44 +0.9 1,329,857 + MMJJ_RS07255 0.44 -0.4 1,329,857 + MMJJ_RS07255 0.44 +1.3 1,329,857 + MMJJ_RS07255 0.44 -1.1 1,329,857 + MMJJ_RS07255 0.44 -0.7 1,329,857 + MMJJ_RS07255 0.44 -0.7 1,329,864 - MMJJ_RS07255 0.44 -0.1 1,329,898 - MMJJ_RS07255 0.48 -0.8 1,329,898 - MMJJ_RS07255 0.48 -0.1 1,329,899 + MMJJ_RS07255 0.48 +0.4 1,329,899 + MMJJ_RS07255 0.48 -1.1 1,329,901 - MMJJ_RS07255 0.48 -0.3 1,329,912 - MMJJ_RS07255 0.49 -0.5 1,329,913 + MMJJ_RS07255 0.49 +0.2 1,329,913 + MMJJ_RS07255 0.49 -0.1 1,329,968 - MMJJ_RS07255 0.54 -0.3 1,329,968 - MMJJ_RS07255 0.54 +0.1 1,329,969 + MMJJ_RS07255 0.54 +1.5 1,329,970 - MMJJ_RS07255 0.54 +0.8 1,329,970 - MMJJ_RS07255 0.54 -0.4 1,329,971 + MMJJ_RS07255 0.55 +0.6 1,329,971 + MMJJ_RS07255 0.55 -1.5 1,330,021 - MMJJ_RS07255 0.59 +0.2 1,330,022 + MMJJ_RS07255 0.59 -0.1 1,330,076 - MMJJ_RS07255 0.64 +0.3 1,330,076 - MMJJ_RS07255 0.64 +0.7 1,330,076 - MMJJ_RS07255 0.64 -0.5 1,330,076 - MMJJ_RS07255 0.64 +1.3 1,330,076 - MMJJ_RS07255 0.64 +1.2 1,330,076 - MMJJ_RS07255 0.64 +1.0 1,330,077 + MMJJ_RS07255 0.65 -1.3 1,330,077 + MMJJ_RS07255 0.65 +0.4 1,330,079 - MMJJ_RS07255 0.65 +0.6 1,330,080 + MMJJ_RS07255 0.65 -0.1 1,330,080 + MMJJ_RS07255 0.65 -0.7 1,330,082 - MMJJ_RS07255 0.65 -0.7 1,330,082 - MMJJ_RS07255 0.65 -0.9 1,330,082 - MMJJ_RS07255 0.65 +0.2 1,330,083 + MMJJ_RS07255 0.65 +0.7 1,330,083 + MMJJ_RS07255 0.65 -0.1 1,330,083 + MMJJ_RS07255 0.65 +0.7 1,330,084 - MMJJ_RS07255 0.65 +1.5 1,330,121 + MMJJ_RS07255 0.69 -0.6 1,330,121 + MMJJ_RS07255 0.69 -0.9 1,330,124 - MMJJ_RS07255 0.69 -0.4 1,330,124 - MMJJ_RS07255 0.69 -0.1 1,330,125 + MMJJ_RS07255 0.69 +0.3 1,330,162 - MMJJ_RS07255 0.73 -1.2 1,330,162 - MMJJ_RS07255 0.73 -1.2 1,330,162 - MMJJ_RS07255 0.73 -0.7 1,330,169 - MMJJ_RS07255 0.73 -1.0 1,330,170 + MMJJ_RS07255 0.73 +1.4 1,330,172 - MMJJ_RS07255 0.74 -1.9 1,330,172 - MMJJ_RS07255 0.74 +0.1 1,330,173 + MMJJ_RS07255 0.74 +0.7 1,330,173 + MMJJ_RS07255 0.74 +0.8 1,330,173 + MMJJ_RS07255 0.74 -0.0
Or see this region's nucleotide sequence