Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS09040 and MMJJ_RS09045 are separated by 52 nucleotides MMJJ_RS09045 and MMJJ_RS09050 are separated by 114 nucleotides MMJJ_RS09050 and MMJJ_RS09055 are separated by 87 nucleotides
MMJJ_RS09040: MMJJ_RS09040 - adenylate kinase, at 1,670,469 to 1,671,047
_RS09040
MMJJ_RS09045: MMJJ_RS09045 - EMC3/TMCO1 family protein, at 1,671,100 to 1,671,696
_RS09045
MMJJ_RS09050: MMJJ_RS09050 - hypothetical protein, at 1,671,811 to 1,672,170
_RS09050
MMJJ_RS09055: MMJJ_RS09055 - NFACT family protein, at 1,672,258 to 1,674,300
_RS09055
Position (kb)
1671
1672
1673 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1671.050 kb on - strand at 1671.056 kb on - strand at 1671.513 kb on + strand, within MMJJ_RS09045 at 1671.672 kb on - strand at 1671.673 kb on + strand at 1671.673 kb on + strand at 1671.681 kb on + strand at 1671.713 kb on + strand at 1671.717 kb on - strand at 1671.717 kb on - strand at 1671.717 kb on - strand at 1671.718 kb on + strand at 1671.718 kb on + strand at 1671.728 kb on - strand at 1671.755 kb on + strand at 1671.760 kb on + strand at 1671.767 kb on + strand at 1671.788 kb on + strand at 1671.788 kb on + strand at 1671.819 kb on + strand at 1671.867 kb on - strand, within MMJJ_RS09050 at 1671.945 kb on - strand, within MMJJ_RS09050 at 1671.991 kb on + strand, within MMJJ_RS09050 at 1672.052 kb on - strand, within MMJJ_RS09050 at 1672.053 kb on + strand, within MMJJ_RS09050 at 1672.138 kb on - strand at 1672.139 kb on + strand at 1672.162 kb on + strand at 1672.187 kb on + strand at 1672.197 kb on - strand at 1672.305 kb on - strand at 1672.388 kb on + strand at 1672.390 kb on - strand at 1672.437 kb on - strand at 1672.437 kb on - strand at 1672.437 kb on - strand at 1672.437 kb on - strand at 1672.441 kb on - strand at 1672.442 kb on + strand at 1672.547 kb on - strand, within MMJJ_RS09055 at 1672.548 kb on + strand, within MMJJ_RS09055 at 1672.684 kb on - strand, within MMJJ_RS09055 at 1672.707 kb on - strand, within MMJJ_RS09055 at 1672.707 kb on - strand, within MMJJ_RS09055 at 1672.717 kb on + strand, within MMJJ_RS09055 at 1672.750 kb on - strand, within MMJJ_RS09055 at 1672.750 kb on - strand, within MMJJ_RS09055 at 1672.750 kb on - strand, within MMJJ_RS09055 at 1672.750 kb on - strand, within MMJJ_RS09055 at 1672.751 kb on + strand, within MMJJ_RS09055 at 1672.769 kb on - strand, within MMJJ_RS09055 at 1672.820 kb on + strand, within MMJJ_RS09055 at 1672.942 kb on - strand, within MMJJ_RS09055 at 1672.973 kb on + strand, within MMJJ_RS09055 at 1673.029 kb on - strand, within MMJJ_RS09055 at 1673.030 kb on + strand, within MMJJ_RS09055 at 1673.030 kb on + strand, within MMJJ_RS09055 at 1673.031 kb on - strand, within MMJJ_RS09055 at 1673.031 kb on - strand, within MMJJ_RS09055
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,671,050 - -3.0 1,671,056 - -0.7 1,671,513 + MMJJ_RS09045 0.69 -1.7 1,671,672 - -2.7 1,671,673 + -1.1 1,671,673 + -0.7 1,671,681 + -0.5 1,671,713 + -0.2 1,671,717 - +1.2 1,671,717 - +0.8 1,671,717 - +1.2 1,671,718 + +0.1 1,671,718 + +1.3 1,671,728 - -1.0 1,671,755 + -0.4 1,671,760 + +1.2 1,671,767 + +0.5 1,671,788 + -0.0 1,671,788 + -0.8 1,671,819 + -0.6 1,671,867 - MMJJ_RS09050 0.16 +0.9 1,671,945 - MMJJ_RS09050 0.37 +1.0 1,671,991 + MMJJ_RS09050 0.50 +1.6 1,672,052 - MMJJ_RS09050 0.67 +0.4 1,672,053 + MMJJ_RS09050 0.67 +0.2 1,672,138 - +0.6 1,672,139 + -0.0 1,672,162 + +1.1 1,672,187 + +0.2 1,672,197 - +0.8 1,672,305 - -2.7 1,672,388 + -0.6 1,672,390 - -2.5 1,672,437 - -0.0 1,672,437 - -3.0 1,672,437 - -0.3 1,672,437 - -0.7 1,672,441 - -2.0 1,672,442 + -1.7 1,672,547 - MMJJ_RS09055 0.14 -3.4 1,672,548 + MMJJ_RS09055 0.14 -2.4 1,672,684 - MMJJ_RS09055 0.21 -0.7 1,672,707 - MMJJ_RS09055 0.22 -1.8 1,672,707 - MMJJ_RS09055 0.22 -2.0 1,672,717 + MMJJ_RS09055 0.22 -1.5 1,672,750 - MMJJ_RS09055 0.24 -4.1 1,672,750 - MMJJ_RS09055 0.24 -1.3 1,672,750 - MMJJ_RS09055 0.24 -2.3 1,672,750 - MMJJ_RS09055 0.24 -0.9 1,672,751 + MMJJ_RS09055 0.24 -1.8 1,672,769 - MMJJ_RS09055 0.25 -2.8 1,672,820 + MMJJ_RS09055 0.28 -3.6 1,672,942 - MMJJ_RS09055 0.33 +1.3 1,672,973 + MMJJ_RS09055 0.35 -3.9 1,673,029 - MMJJ_RS09055 0.38 -2.4 1,673,030 + MMJJ_RS09055 0.38 -0.2 1,673,030 + MMJJ_RS09055 0.38 -1.0 1,673,031 - MMJJ_RS09055 0.38 -1.7 1,673,031 - MMJJ_RS09055 0.38 +1.0
Or see this region's nucleotide sequence