Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07900 and MMJJ_RS07905 are separated by 62 nucleotides MMJJ_RS07905 and MMJJ_RS07910 are separated by 120 nucleotides MMJJ_RS07910 and MMJJ_RS07915 are separated by 22 nucleotides
MMJJ_RS07900: MMJJ_RS07900 - phosphoribosylformylglycinamidine cyclo-ligase, at 1,450,019 to 1,451,068
_RS07900
MMJJ_RS07905: MMJJ_RS07905 - DUF89 domain-containing protein, at 1,451,131 to 1,452,030
_RS07905
MMJJ_RS07910: MMJJ_RS07910 - CBS domain-containing protein, at 1,452,151 to 1,452,990
_RS07910
MMJJ_RS07915: MMJJ_RS07915 - CBS domain-containing protein, at 1,453,013 to 1,453,573
_RS07915
Position (kb)
1451
1452
1453 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1450.184 kb on - strand, within MMJJ_RS07900 at 1450.305 kb on + strand, within MMJJ_RS07900 at 1451.058 kb on - strand at 1451.139 kb on - strand at 1451.139 kb on - strand at 1451.140 kb on + strand at 1451.143 kb on - strand at 1451.179 kb on - strand at 1451.180 kb on + strand at 1451.223 kb on - strand, within MMJJ_RS07905 at 1451.223 kb on - strand, within MMJJ_RS07905 at 1451.224 kb on + strand, within MMJJ_RS07905 at 1451.229 kb on + strand, within MMJJ_RS07905 at 1451.329 kb on - strand, within MMJJ_RS07905 at 1451.398 kb on - strand, within MMJJ_RS07905 at 1451.399 kb on + strand, within MMJJ_RS07905 at 1451.407 kb on - strand, within MMJJ_RS07905 at 1451.558 kb on - strand, within MMJJ_RS07905 at 1451.563 kb on - strand, within MMJJ_RS07905 at 1451.581 kb on + strand, within MMJJ_RS07905 at 1451.617 kb on - strand, within MMJJ_RS07905 at 1451.642 kb on + strand, within MMJJ_RS07905 at 1451.647 kb on + strand, within MMJJ_RS07905 at 1451.647 kb on + strand, within MMJJ_RS07905 at 1451.671 kb on + strand, within MMJJ_RS07905 at 1451.671 kb on + strand, within MMJJ_RS07905 at 1451.781 kb on - strand, within MMJJ_RS07905 at 1451.782 kb on + strand, within MMJJ_RS07905 at 1451.830 kb on + strand, within MMJJ_RS07905 at 1451.849 kb on + strand, within MMJJ_RS07905 at 1451.849 kb on + strand, within MMJJ_RS07905 at 1451.852 kb on - strand, within MMJJ_RS07905 at 1451.853 kb on + strand, within MMJJ_RS07905 at 1451.875 kb on + strand, within MMJJ_RS07905 at 1451.875 kb on + strand, within MMJJ_RS07905 at 1451.900 kb on - strand, within MMJJ_RS07905 at 1451.933 kb on + strand, within MMJJ_RS07905 at 1451.934 kb on - strand, within MMJJ_RS07905 at 1451.935 kb on + strand, within MMJJ_RS07905 at 1451.956 kb on + strand at 1451.966 kb on + strand at 1451.983 kb on - strand at 1451.984 kb on + strand at 1451.984 kb on + strand at 1451.984 kb on + strand at 1451.984 kb on + strand at 1452.022 kb on + strand at 1452.049 kb on - strand at 1452.049 kb on - strand at 1452.049 kb on - strand at 1452.049 kb on - strand at 1452.049 kb on - strand at 1452.050 kb on + strand at 1452.050 kb on + strand at 1452.050 kb on + strand at 1452.053 kb on + strand at 1452.070 kb on + strand at 1452.072 kb on - strand at 1452.073 kb on + strand at 1452.073 kb on + strand at 1452.075 kb on - strand at 1452.075 kb on - strand at 1452.075 kb on - strand at 1452.075 kb on - strand at 1452.076 kb on + strand at 1452.079 kb on - strand at 1452.079 kb on - strand at 1452.080 kb on + strand at 1452.134 kb on + strand at 1452.149 kb on + strand at 1452.152 kb on - strand at 1452.153 kb on + strand at 1452.161 kb on - strand at 1452.172 kb on - strand at 1452.172 kb on - strand at 1452.220 kb on - strand at 1452.238 kb on + strand, within MMJJ_RS07910 at 1452.242 kb on + strand, within MMJJ_RS07910 at 1452.259 kb on + strand, within MMJJ_RS07910 at 1452.277 kb on + strand, within MMJJ_RS07910 at 1452.277 kb on + strand, within MMJJ_RS07910 at 1452.300 kb on - strand, within MMJJ_RS07910 at 1452.301 kb on + strand, within MMJJ_RS07910 at 1452.301 kb on + strand, within MMJJ_RS07910 at 1452.337 kb on + strand, within MMJJ_RS07910 at 1452.520 kb on - strand, within MMJJ_RS07910 at 1452.520 kb on - strand, within MMJJ_RS07910 at 1452.523 kb on - strand, within MMJJ_RS07910 at 1452.547 kb on - strand, within MMJJ_RS07910 at 1452.669 kb on + strand, within MMJJ_RS07910 at 1452.669 kb on + strand, within MMJJ_RS07910 at 1452.669 kb on + strand, within MMJJ_RS07910 at 1452.670 kb on - strand, within MMJJ_RS07910 at 1452.671 kb on + strand, within MMJJ_RS07910 at 1452.679 kb on - strand, within MMJJ_RS07910 at 1452.706 kb on - strand, within MMJJ_RS07910 at 1452.709 kb on - strand, within MMJJ_RS07910 at 1452.710 kb on + strand, within MMJJ_RS07910 at 1452.712 kb on - strand, within MMJJ_RS07910 at 1452.798 kb on + strand, within MMJJ_RS07910 at 1452.802 kb on + strand, within MMJJ_RS07910 at 1452.854 kb on + strand, within MMJJ_RS07910 at 1452.854 kb on + strand, within MMJJ_RS07910 at 1452.883 kb on - strand, within MMJJ_RS07910 at 1452.883 kb on - strand, within MMJJ_RS07910 at 1452.884 kb on + strand, within MMJJ_RS07910 at 1452.884 kb on + strand, within MMJJ_RS07910 at 1452.927 kb on - strand at 1452.939 kb on - strand at 1452.939 kb on - strand at 1452.940 kb on + strand at 1452.940 kb on + strand at 1452.946 kb on - strand at 1453.006 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,450,184 - MMJJ_RS07900 0.16 -2.8 1,450,305 + MMJJ_RS07900 0.27 -1.3 1,451,058 - -1.3 1,451,139 - -1.1 1,451,139 - +0.4 1,451,140 + -0.5 1,451,143 - +1.8 1,451,179 - +0.8 1,451,180 + +1.0 1,451,223 - MMJJ_RS07905 0.10 +0.1 1,451,223 - MMJJ_RS07905 0.10 +0.0 1,451,224 + MMJJ_RS07905 0.10 -1.2 1,451,229 + MMJJ_RS07905 0.11 +0.1 1,451,329 - MMJJ_RS07905 0.22 +0.6 1,451,398 - MMJJ_RS07905 0.30 -0.5 1,451,399 + MMJJ_RS07905 0.30 +0.8 1,451,407 - MMJJ_RS07905 0.31 +1.2 1,451,558 - MMJJ_RS07905 0.47 -0.1 1,451,563 - MMJJ_RS07905 0.48 +2.2 1,451,581 + MMJJ_RS07905 0.50 +1.7 1,451,617 - MMJJ_RS07905 0.54 +1.1 1,451,642 + MMJJ_RS07905 0.57 -0.5 1,451,647 + MMJJ_RS07905 0.57 +1.1 1,451,647 + MMJJ_RS07905 0.57 -0.5 1,451,671 + MMJJ_RS07905 0.60 +0.9 1,451,671 + MMJJ_RS07905 0.60 +0.8 1,451,781 - MMJJ_RS07905 0.72 +4.1 1,451,782 + MMJJ_RS07905 0.72 +0.6 1,451,830 + MMJJ_RS07905 0.78 +1.1 1,451,849 + MMJJ_RS07905 0.80 -0.3 1,451,849 + MMJJ_RS07905 0.80 +0.5 1,451,852 - MMJJ_RS07905 0.80 -0.3 1,451,853 + MMJJ_RS07905 0.80 +1.2 1,451,875 + MMJJ_RS07905 0.83 +0.0 1,451,875 + MMJJ_RS07905 0.83 -0.0 1,451,900 - MMJJ_RS07905 0.85 -0.9 1,451,933 + MMJJ_RS07905 0.89 -0.1 1,451,934 - MMJJ_RS07905 0.89 +1.9 1,451,935 + MMJJ_RS07905 0.89 +1.0 1,451,956 + -2.9 1,451,966 + -1.8 1,451,983 - +0.7 1,451,984 + +1.0 1,451,984 + -0.3 1,451,984 + +0.2 1,451,984 + +1.4 1,452,022 + +1.5 1,452,049 - -0.7 1,452,049 - -0.5 1,452,049 - +0.1 1,452,049 - +1.7 1,452,049 - +0.0 1,452,050 + +0.2 1,452,050 + +1.1 1,452,050 + +0.8 1,452,053 + +0.9 1,452,070 + -0.1 1,452,072 - +0.3 1,452,073 + +1.3 1,452,073 + -2.0 1,452,075 - -2.0 1,452,075 - +0.9 1,452,075 - +0.9 1,452,075 - +0.0 1,452,076 + +0.1 1,452,079 - +1.3 1,452,079 - -0.2 1,452,080 + -0.5 1,452,134 + +0.3 1,452,149 + +0.9 1,452,152 - -0.2 1,452,153 + -0.5 1,452,161 - +0.5 1,452,172 - +0.0 1,452,172 - +0.2 1,452,220 - +1.0 1,452,238 + MMJJ_RS07910 0.10 +0.1 1,452,242 + MMJJ_RS07910 0.11 +0.0 1,452,259 + MMJJ_RS07910 0.13 +1.1 1,452,277 + MMJJ_RS07910 0.15 +0.5 1,452,277 + MMJJ_RS07910 0.15 -1.7 1,452,300 - MMJJ_RS07910 0.18 -0.4 1,452,301 + MMJJ_RS07910 0.18 -0.8 1,452,301 + MMJJ_RS07910 0.18 +1.3 1,452,337 + MMJJ_RS07910 0.22 -1.1 1,452,520 - MMJJ_RS07910 0.44 +1.3 1,452,520 - MMJJ_RS07910 0.44 +1.0 1,452,523 - MMJJ_RS07910 0.44 -1.9 1,452,547 - MMJJ_RS07910 0.47 +1.2 1,452,669 + MMJJ_RS07910 0.62 +0.3 1,452,669 + MMJJ_RS07910 0.62 -0.1 1,452,669 + MMJJ_RS07910 0.62 +1.0 1,452,670 - MMJJ_RS07910 0.62 -0.7 1,452,671 + MMJJ_RS07910 0.62 +0.5 1,452,679 - MMJJ_RS07910 0.63 -1.2 1,452,706 - MMJJ_RS07910 0.66 +1.4 1,452,709 - MMJJ_RS07910 0.66 +0.4 1,452,710 + MMJJ_RS07910 0.67 +0.2 1,452,712 - MMJJ_RS07910 0.67 +0.9 1,452,798 + MMJJ_RS07910 0.77 +0.5 1,452,802 + MMJJ_RS07910 0.78 -1.1 1,452,854 + MMJJ_RS07910 0.84 -0.7 1,452,854 + MMJJ_RS07910 0.84 -0.2 1,452,883 - MMJJ_RS07910 0.87 +0.3 1,452,883 - MMJJ_RS07910 0.87 +0.6 1,452,884 + MMJJ_RS07910 0.87 -0.4 1,452,884 + MMJJ_RS07910 0.87 -0.0 1,452,927 - +0.9 1,452,939 - +1.0 1,452,939 - -0.5 1,452,940 + -0.2 1,452,940 + +1.6 1,452,946 - +0.6 1,453,006 - +1.0
Or see this region's nucleotide sequence