Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07820 and MMJJ_RS07825 are separated by 39 nucleotides MMJJ_RS07825 and MMJJ_RS07830 are separated by 17 nucleotides MMJJ_RS07830 and MMJJ_RS07835 are separated by 138 nucleotides MMJJ_RS07835 and MMJJ_RS07840 are separated by 96 nucleotides
MMJJ_RS07820: MMJJ_RS07820 - bifunctional hexulose-6-phosphate synthase/ribonuclease regulator, at 1,432,803 to 1,434,119
_RS07820
MMJJ_RS07825: MMJJ_RS07825 - YciI family protein, at 1,434,159 to 1,434,449
_RS07825
MMJJ_RS07830: MMJJ_RS07830 - DUF1786 domain-containing protein, at 1,434,467 to 1,435,483
_RS07830
MMJJ_RS07835: MMJJ_RS07835 - UPF0489 family protein, at 1,435,622 to 1,436,332
_RS07835
MMJJ_RS07840: MMJJ_RS07840 - Glu-tRNA(Gln) amidotransferase subunit GatD, at 1,436,429 to 1,437,685
_RS07840
Position (kb)
1434
1435
1436 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1433.525 kb on + strand, within MMJJ_RS07820 at 1433.539 kb on - strand, within MMJJ_RS07820 at 1433.556 kb on + strand, within MMJJ_RS07820 at 1433.636 kb on - strand, within MMJJ_RS07820 at 1433.643 kb on - strand, within MMJJ_RS07820 at 1433.669 kb on + strand, within MMJJ_RS07820 at 1433.669 kb on + strand, within MMJJ_RS07820 at 1433.704 kb on - strand, within MMJJ_RS07820 at 1433.704 kb on - strand, within MMJJ_RS07820 at 1433.705 kb on + strand, within MMJJ_RS07820 at 1433.705 kb on + strand, within MMJJ_RS07820 at 1433.708 kb on + strand, within MMJJ_RS07820 at 1433.708 kb on + strand, within MMJJ_RS07820 at 1433.792 kb on + strand, within MMJJ_RS07820 at 1433.792 kb on + strand, within MMJJ_RS07820 at 1433.792 kb on + strand, within MMJJ_RS07820 at 1433.794 kb on - strand, within MMJJ_RS07820 at 1433.794 kb on - strand, within MMJJ_RS07820 at 1433.795 kb on + strand, within MMJJ_RS07820 at 1433.795 kb on + strand, within MMJJ_RS07820 at 1433.858 kb on - strand, within MMJJ_RS07820 at 1433.859 kb on + strand, within MMJJ_RS07820 at 1433.859 kb on + strand, within MMJJ_RS07820 at 1433.859 kb on + strand, within MMJJ_RS07820 at 1433.859 kb on + strand, within MMJJ_RS07820 at 1433.861 kb on + strand, within MMJJ_RS07820 at 1433.899 kb on - strand, within MMJJ_RS07820 at 1433.900 kb on + strand, within MMJJ_RS07820 at 1433.972 kb on - strand, within MMJJ_RS07820 at 1433.977 kb on - strand, within MMJJ_RS07820 at 1434.041 kb on - strand at 1434.042 kb on + strand at 1434.130 kb on - strand at 1434.131 kb on + strand at 1434.229 kb on - strand, within MMJJ_RS07825 at 1434.244 kb on - strand, within MMJJ_RS07825 at 1434.262 kb on + strand, within MMJJ_RS07825 at 1434.291 kb on + strand, within MMJJ_RS07825 at 1434.342 kb on - strand, within MMJJ_RS07825 at 1434.342 kb on - strand, within MMJJ_RS07825 at 1434.362 kb on + strand, within MMJJ_RS07825 at 1434.362 kb on + strand, within MMJJ_RS07825 at 1434.383 kb on - strand, within MMJJ_RS07825 at 1434.384 kb on + strand, within MMJJ_RS07825 at 1434.441 kb on - strand at 1434.442 kb on + strand at 1434.442 kb on + strand at 1434.443 kb on - strand at 1434.443 kb on - strand at 1434.444 kb on + strand at 1434.444 kb on + strand at 1434.497 kb on - strand at 1434.498 kb on + strand at 1434.524 kb on - strand at 1434.547 kb on + strand at 1434.614 kb on + strand, within MMJJ_RS07830 at 1434.647 kb on + strand, within MMJJ_RS07830 at 1434.647 kb on + strand, within MMJJ_RS07830 at 1434.648 kb on - strand, within MMJJ_RS07830 at 1434.648 kb on - strand, within MMJJ_RS07830 at 1434.648 kb on - strand, within MMJJ_RS07830 at 1434.649 kb on + strand, within MMJJ_RS07830 at 1434.649 kb on + strand, within MMJJ_RS07830 at 1434.650 kb on - strand, within MMJJ_RS07830 at 1434.650 kb on - strand, within MMJJ_RS07830 at 1434.650 kb on - strand, within MMJJ_RS07830 at 1434.651 kb on + strand, within MMJJ_RS07830 at 1434.651 kb on + strand, within MMJJ_RS07830 at 1434.651 kb on + strand, within MMJJ_RS07830 at 1434.651 kb on + strand, within MMJJ_RS07830 at 1434.651 kb on + strand, within MMJJ_RS07830 at 1434.651 kb on + strand, within MMJJ_RS07830 at 1434.651 kb on + strand, within MMJJ_RS07830 at 1434.653 kb on + strand, within MMJJ_RS07830 at 1434.701 kb on - strand, within MMJJ_RS07830 at 1434.781 kb on - strand, within MMJJ_RS07830 at 1434.781 kb on - strand, within MMJJ_RS07830 at 1434.843 kb on + strand, within MMJJ_RS07830 at 1434.892 kb on + strand, within MMJJ_RS07830 at 1434.896 kb on + strand, within MMJJ_RS07830 at 1434.914 kb on - strand, within MMJJ_RS07830 at 1434.958 kb on - strand, within MMJJ_RS07830 at 1434.958 kb on - strand, within MMJJ_RS07830 at 1434.958 kb on - strand, within MMJJ_RS07830 at 1435.019 kb on + strand, within MMJJ_RS07830 at 1435.073 kb on - strand, within MMJJ_RS07830 at 1435.073 kb on - strand, within MMJJ_RS07830 at 1435.074 kb on + strand, within MMJJ_RS07830 at 1435.074 kb on + strand, within MMJJ_RS07830 at 1435.158 kb on + strand, within MMJJ_RS07830 at 1435.185 kb on + strand, within MMJJ_RS07830 at 1435.274 kb on - strand, within MMJJ_RS07830 at 1435.392 kb on + strand at 1435.487 kb on + strand at 1435.536 kb on + strand at 1435.541 kb on - strand at 1435.541 kb on - strand at 1435.549 kb on - strand at 1435.550 kb on + strand at 1435.552 kb on + strand at 1435.570 kb on - strand at 1435.570 kb on - strand at 1435.570 kb on - strand at 1435.570 kb on - strand at 1435.571 kb on + strand at 1435.571 kb on + strand at 1435.571 kb on + strand at 1435.571 kb on + strand at 1435.571 kb on + strand at 1435.573 kb on + strand at 1435.584 kb on - strand at 1435.585 kb on + strand at 1435.603 kb on + strand at 1435.621 kb on - strand at 1435.621 kb on - strand at 1435.657 kb on + strand at 1435.820 kb on - strand, within MMJJ_RS07835 at 1435.919 kb on - strand at 1435.925 kb on - strand, within MMJJ_RS07835 at 1435.925 kb on - strand, within MMJJ_RS07835 at 1435.925 kb on - strand, within MMJJ_RS07835 at 1435.925 kb on - strand, within MMJJ_RS07835 at 1435.925 kb on - strand, within MMJJ_RS07835 at 1435.926 kb on + strand, within MMJJ_RS07835 at 1435.926 kb on + strand, within MMJJ_RS07835 at 1435.926 kb on + strand, within MMJJ_RS07835 at 1435.926 kb on + strand, within MMJJ_RS07835 at 1435.926 kb on + strand, within MMJJ_RS07835 at 1435.928 kb on + strand, within MMJJ_RS07835 at 1435.985 kb on - strand, within MMJJ_RS07835 at 1435.997 kb on - strand, within MMJJ_RS07835 at 1436.033 kb on + strand, within MMJJ_RS07835 at 1436.038 kb on + strand, within MMJJ_RS07835 at 1436.042 kb on - strand, within MMJJ_RS07835 at 1436.042 kb on - strand, within MMJJ_RS07835 at 1436.043 kb on + strand, within MMJJ_RS07835 at 1436.047 kb on - strand, within MMJJ_RS07835 at 1436.048 kb on + strand, within MMJJ_RS07835 at 1436.074 kb on + strand, within MMJJ_RS07835 at 1436.250 kb on - strand, within MMJJ_RS07835 at 1436.260 kb on + strand, within MMJJ_RS07835 at 1436.270 kb on - strand at 1436.287 kb on + strand at 1436.290 kb on - strand at 1436.291 kb on + strand at 1436.291 kb on + strand at 1436.291 kb on + strand at 1436.291 kb on + strand at 1436.296 kb on + strand at 1436.316 kb on + strand at 1436.317 kb on - strand at 1436.318 kb on + strand at 1436.318 kb on + strand at 1436.331 kb on + strand at 1436.331 kb on + strand at 1436.334 kb on - strand at 1436.371 kb on + strand at 1436.384 kb on + strand at 1436.384 kb on + strand at 1436.384 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,433,525 + MMJJ_RS07820 0.55 +1.7 1,433,539 - MMJJ_RS07820 0.56 +0.4 1,433,556 + MMJJ_RS07820 0.57 -0.7 1,433,636 - MMJJ_RS07820 0.63 +1.1 1,433,643 - MMJJ_RS07820 0.64 -0.1 1,433,669 + MMJJ_RS07820 0.66 -0.6 1,433,669 + MMJJ_RS07820 0.66 +1.1 1,433,704 - MMJJ_RS07820 0.68 +0.0 1,433,704 - MMJJ_RS07820 0.68 -0.9 1,433,705 + MMJJ_RS07820 0.68 -0.8 1,433,705 + MMJJ_RS07820 0.68 -0.1 1,433,708 + MMJJ_RS07820 0.69 +0.2 1,433,708 + MMJJ_RS07820 0.69 -0.6 1,433,792 + MMJJ_RS07820 0.75 +0.8 1,433,792 + MMJJ_RS07820 0.75 -0.1 1,433,792 + MMJJ_RS07820 0.75 -0.5 1,433,794 - MMJJ_RS07820 0.75 -0.9 1,433,794 - MMJJ_RS07820 0.75 -0.7 1,433,795 + MMJJ_RS07820 0.75 +0.3 1,433,795 + MMJJ_RS07820 0.75 +0.5 1,433,858 - MMJJ_RS07820 0.80 -0.1 1,433,859 + MMJJ_RS07820 0.80 -0.7 1,433,859 + MMJJ_RS07820 0.80 -1.2 1,433,859 + MMJJ_RS07820 0.80 -0.9 1,433,859 + MMJJ_RS07820 0.80 +0.0 1,433,861 + MMJJ_RS07820 0.80 -0.1 1,433,899 - MMJJ_RS07820 0.83 -0.2 1,433,900 + MMJJ_RS07820 0.83 -0.7 1,433,972 - MMJJ_RS07820 0.89 +0.1 1,433,977 - MMJJ_RS07820 0.89 +0.9 1,434,041 - +0.8 1,434,042 + -2.5 1,434,130 - -0.2 1,434,131 + +0.9 1,434,229 - MMJJ_RS07825 0.24 +1.0 1,434,244 - MMJJ_RS07825 0.29 -1.7 1,434,262 + MMJJ_RS07825 0.35 -0.2 1,434,291 + MMJJ_RS07825 0.45 +0.6 1,434,342 - MMJJ_RS07825 0.63 +0.5 1,434,342 - MMJJ_RS07825 0.63 +1.8 1,434,362 + MMJJ_RS07825 0.70 +1.2 1,434,362 + MMJJ_RS07825 0.70 +0.7 1,434,383 - MMJJ_RS07825 0.77 +0.7 1,434,384 + MMJJ_RS07825 0.77 +1.2 1,434,441 - -0.4 1,434,442 + +1.0 1,434,442 + +1.9 1,434,443 - -1.0 1,434,443 - -1.0 1,434,444 + +1.0 1,434,444 + -1.4 1,434,497 - -0.2 1,434,498 + +0.0 1,434,524 - -0.4 1,434,547 + -0.2 1,434,614 + MMJJ_RS07830 0.14 +0.6 1,434,647 + MMJJ_RS07830 0.18 +0.7 1,434,647 + MMJJ_RS07830 0.18 +1.4 1,434,648 - MMJJ_RS07830 0.18 -1.6 1,434,648 - MMJJ_RS07830 0.18 -0.4 1,434,648 - MMJJ_RS07830 0.18 -1.4 1,434,649 + MMJJ_RS07830 0.18 -0.3 1,434,649 + MMJJ_RS07830 0.18 +0.6 1,434,650 - MMJJ_RS07830 0.18 -2.0 1,434,650 - MMJJ_RS07830 0.18 -0.5 1,434,650 - MMJJ_RS07830 0.18 +0.8 1,434,651 + MMJJ_RS07830 0.18 -0.1 1,434,651 + MMJJ_RS07830 0.18 +0.4 1,434,651 + MMJJ_RS07830 0.18 -1.5 1,434,651 + MMJJ_RS07830 0.18 -1.5 1,434,651 + MMJJ_RS07830 0.18 -0.2 1,434,651 + MMJJ_RS07830 0.18 +1.0 1,434,651 + MMJJ_RS07830 0.18 -0.5 1,434,653 + MMJJ_RS07830 0.18 +0.7 1,434,701 - MMJJ_RS07830 0.23 +0.9 1,434,781 - MMJJ_RS07830 0.31 +0.7 1,434,781 - MMJJ_RS07830 0.31 +1.0 1,434,843 + MMJJ_RS07830 0.37 +1.9 1,434,892 + MMJJ_RS07830 0.42 +1.1 1,434,896 + MMJJ_RS07830 0.42 +1.2 1,434,914 - MMJJ_RS07830 0.44 +0.9 1,434,958 - MMJJ_RS07830 0.48 -0.6 1,434,958 - MMJJ_RS07830 0.48 -0.1 1,434,958 - MMJJ_RS07830 0.48 -0.2 1,435,019 + MMJJ_RS07830 0.54 +0.3 1,435,073 - MMJJ_RS07830 0.60 +1.1 1,435,073 - MMJJ_RS07830 0.60 -0.1 1,435,074 + MMJJ_RS07830 0.60 -0.4 1,435,074 + MMJJ_RS07830 0.60 -0.9 1,435,158 + MMJJ_RS07830 0.68 +1.0 1,435,185 + MMJJ_RS07830 0.71 +1.5 1,435,274 - MMJJ_RS07830 0.79 +0.2 1,435,392 + +0.2 1,435,487 + +0.9 1,435,536 + +1.7 1,435,541 - -0.4 1,435,541 - +0.6 1,435,549 - -0.4 1,435,550 + +0.3 1,435,552 + -0.8 1,435,570 - -0.3 1,435,570 - -0.0 1,435,570 - -0.4 1,435,570 - +0.4 1,435,571 + +1.0 1,435,571 + +0.1 1,435,571 + +0.2 1,435,571 + +0.8 1,435,571 + +0.9 1,435,573 + +0.2 1,435,584 - +0.7 1,435,585 + -0.9 1,435,603 + +0.9 1,435,621 - +0.3 1,435,621 - -1.1 1,435,657 + -2.2 1,435,820 - MMJJ_RS07835 0.28 -0.3 1,435,919 - -1.0 1,435,925 - MMJJ_RS07835 0.43 -0.6 1,435,925 - MMJJ_RS07835 0.43 -0.5 1,435,925 - MMJJ_RS07835 0.43 +0.5 1,435,925 - MMJJ_RS07835 0.43 +0.1 1,435,925 - MMJJ_RS07835 0.43 +0.9 1,435,926 + MMJJ_RS07835 0.43 -0.2 1,435,926 + MMJJ_RS07835 0.43 +0.8 1,435,926 + MMJJ_RS07835 0.43 -0.7 1,435,926 + MMJJ_RS07835 0.43 -1.0 1,435,926 + MMJJ_RS07835 0.43 +0.1 1,435,928 + MMJJ_RS07835 0.43 +0.5 1,435,985 - MMJJ_RS07835 0.51 +0.7 1,435,997 - MMJJ_RS07835 0.53 +0.6 1,436,033 + MMJJ_RS07835 0.58 +0.5 1,436,038 + MMJJ_RS07835 0.59 +0.4 1,436,042 - MMJJ_RS07835 0.59 +0.9 1,436,042 - MMJJ_RS07835 0.59 -0.1 1,436,043 + MMJJ_RS07835 0.59 +1.4 1,436,047 - MMJJ_RS07835 0.60 -3.2 1,436,048 + MMJJ_RS07835 0.60 +0.4 1,436,074 + MMJJ_RS07835 0.64 +1.0 1,436,250 - MMJJ_RS07835 0.88 +0.4 1,436,260 + MMJJ_RS07835 0.90 +0.3 1,436,270 - +0.1 1,436,287 + +0.7 1,436,290 - -0.2 1,436,291 + -2.2 1,436,291 + -0.4 1,436,291 + -1.0 1,436,291 + -2.2 1,436,296 + +0.4 1,436,316 + +1.2 1,436,317 - +0.8 1,436,318 + +0.1 1,436,318 + +0.6 1,436,331 + -1.1 1,436,331 + +0.3 1,436,334 - +1.1 1,436,371 + +0.4 1,436,384 + +0.3 1,436,384 + -0.7 1,436,384 + -0.6
Or see this region's nucleotide sequence